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The map label for this gene is aroE

Identifier: 227080293

GI number: 227080293

Start: 51667

End: 52503

Strand: Direct

Name: aroE

Synonym: VCM66_0056

Alternate gene names: 227080293

Gene position: 51667-52503 (Clockwise)

Preceding gene: 227080292

Following gene: 227080294

Centisome position: 1.79

GC content: 50.3

Gene sequence:

>837_bases
ATGGCTTCTCAAATCGATCAGTATGCCGTTTTTGGTAACCCCATTAATCACAGTAAATCCCCTTTTATTCATACGCTGTT
TGCACGACAAACTCAGCAGTCCATGATCTATACCGCACAGTGCGTGCCAGTGGATGGGTTTACTGAGGCGGCGAAACATT
TTTTTGCGCAAGGTGGACGAGGTTGTAATGTTACCGTGCCATTTAAAGAGGAGGCGTACCGCTTTGCCGATCGCTTAACG
GAGCGCGCGCGTTTAGCCGGTGCGGTAAACACGTTAAAAAAGCTGGATGATGGCGAAATACTCGGAGACAACACCGATGG
TGAAGGCTTAGTGCAAGATCTGCTCGCGCAGCAAGTGCTACTGAAAGGCGCGACGATTTTGTTGATTGGTGCCGGTGGTG
CGGCGCGTGGCGTGCTCAAGCCGTTATTAGATCAGCAGCCCGCCTCTATTACCGTCACGAATCGTACCTTTGCGAAAGCG
GAGCAGTTGGCTGAGCTTGTGGCCGCTTATGGTGAGGTAAAGGCGCAAGCGTTCGAACAGCTTAAACAAAGTTATGATGT
GATCATCAACTCGACCTCTGCCAGTCTCGATGGCGAATTGCCCGCGATTGATCCGGTGATCTTTTCATCTCGCTCCGTCT
GCTATGACATGATGTATGGTAAAGGCTATACCGTGTTTAATCAATGGGCGCGACAACATGGCTGCGCGCAAGCGATCGAT
GGCTTAGGCATGTTAGTGGGACAAGCGGCTGAAAGTTTTATGCTATGGCGTGGTTTACGTCCGGGCACGAAACAAATTTT
GCGTGAACTGCGTAAAAATCTAGAAGGTGCGCTATGA

Upstream 100 bases:

>100_bases
GCCCAGTTGAGCGAGTTTTTAGTTCCGCGTGAATGGTGACACTTGAAGTTCTGAGTGATAGGGTCGCGCAAGTGACCCTT
TTGATTGTTTAGGCAAGGAT

Downstream 100 bases:

>100_bases
ACCAATCCATCTTGTTTCCCGATATACAGCATTGGGATGAGGCAAAACAAGCCGTTGTATTTTTTGCTCAGCAAAACGGA
GCATTAATCGAATGTCTTGT

Product: shikimate 5-dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MASQIDQYAVFGNPINHSKSPFIHTLFARQTQQSMIYTAQCVPVDGFTEAAKHFFAQGGRGCNVTVPFKEEAYRFADRLT
ERARLAGAVNTLKKLDDGEILGDNTDGEGLVQDLLAQQVLLKGATILLIGAGGAARGVLKPLLDQQPASITVTNRTFAKA
EQLAELVAAYGEVKAQAFEQLKQSYDVIINSTSASLDGELPAIDPVIFSSRSVCYDMMYGKGYTVFNQWARQHGCAQAID
GLGMLVGQAAESFMLWRGLRPGTKQILRELRKNLEGAL

Sequences:

>Translated_278_residues
MASQIDQYAVFGNPINHSKSPFIHTLFARQTQQSMIYTAQCVPVDGFTEAAKHFFAQGGRGCNVTVPFKEEAYRFADRLT
ERARLAGAVNTLKKLDDGEILGDNTDGEGLVQDLLAQQVLLKGATILLIGAGGAARGVLKPLLDQQPASITVTNRTFAKA
EQLAELVAAYGEVKAQAFEQLKQSYDVIINSTSASLDGELPAIDPVIFSSRSVCYDMMYGKGYTVFNQWARQHGCAQAID
GLGMLVGQAAESFMLWRGLRPGTKQILRELRKNLEGAL
>Mature_277_residues
ASQIDQYAVFGNPINHSKSPFIHTLFARQTQQSMIYTAQCVPVDGFTEAAKHFFAQGGRGCNVTVPFKEEAYRFADRLTE
RARLAGAVNTLKKLDDGEILGDNTDGEGLVQDLLAQQVLLKGATILLIGAGGAARGVLKPLLDQQPASITVTNRTFAKAE
QLAELVAAYGEVKAQAFEQLKQSYDVIINSTSASLDGELPAIDPVIFSSRSVCYDMMYGKGYTVFNQWARQHGCAQAIDG
LGMLVGQAAESFMLWRGLRPGTKQILRELRKNLEGAL

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; fourth step. [C]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family

Homologues:

Organism=Escherichia coli, GI1789675, Length=271, Percent_Identity=50.1845018450184, Blast_Score=273, Evalue=1e-74,
Organism=Escherichia coli, GI1787983, Length=259, Percent_Identity=32.4324324324324, Blast_Score=103, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6320332, Length=283, Percent_Identity=26.5017667844523, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROE_VIBC3 (A5F4C3)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F4C3
- SMR:   A5F4C3
- STRING:   A5F4C3
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A2464
- eggNOG:   COG0169
- HOGENOM:   HBG553408
- OMA:   DQYVVFG
- ProtClustDB:   PRK00258
- BioCyc:   VCHO345073:VC0395_A2464-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00222
- InterPro:   IPR016040
- InterPro:   IPR011342
- InterPro:   IPR013708
- InterPro:   IPR022893
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR00507

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N

EC number: =1.1.1.25

Molecular weight: Translated: 30250; Mature: 30119

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: ACT_SITE 69-69

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASQIDQYAVFGNPINHSKSPFIHTLFARQTQQSMIYTAQCVPVDGFTEAAKHFFAQGGR
CCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHCCCC
GCNVTVPFKEEAYRFADRLTERARLAGAVNTLKKLDDGEILGDNTDGEGLVQDLLAQQVL
CCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHH
LKGATILLIGAGGAARGVLKPLLDQQPASITVTNRTFAKAEQLAELVAAYGEVKAQAFEQ
HCCCEEEEEECCCHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
LKQSYDVIINSTSASLDGELPAIDPVIFSSRSVCYDMMYGKGYTVFNQWARQHGCAQAID
HHHHHHEEEECCCCCCCCCCCCCCHHHHCCCHHHHHHHHCCCHHHHHHHHHHCCHHHHHH
GLGMLVGQAAESFMLWRGLRPGTKQILRELRKNLEGAL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ASQIDQYAVFGNPINHSKSPFIHTLFARQTQQSMIYTAQCVPVDGFTEAAKHFFAQGGR
CCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHCCCC
GCNVTVPFKEEAYRFADRLTERARLAGAVNTLKKLDDGEILGDNTDGEGLVQDLLAQQVL
CCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHH
LKGATILLIGAGGAARGVLKPLLDQQPASITVTNRTFAKAEQLAELVAAYGEVKAQAFEQ
HCCCEEEEEECCCHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
LKQSYDVIINSTSASLDGELPAIDPVIFSSRSVCYDMMYGKGYTVFNQWARQHGCAQAID
HHHHHHEEEECCCCCCCCCCCCCCHHHHCCCHHHHHHHHCCCHHHHHHHHHHCCHHHHHH
GLGMLVGQAAESFMLWRGLRPGTKQILRELRKNLEGAL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA