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The map label for this gene is glnA [H]

Identifier: 226951019

GI number: 226951019

Start: 4076741

End: 4078639

Strand: Direct

Name: glnA [H]

Synonym: CLM_4056

Alternate gene names: 226951019

Gene position: 4076741-4078639 (Clockwise)

Preceding gene: 226951013

Following gene: 226951021

Centisome position: 98.11

GC content: 30.17

Gene sequence:

>1899_bases
ATGTTAGATGATTTAGTATATGTTATTCCTAAAGAAATGCATTCCGAAGAACAACTAAAAGAATTTTTAGTTTCCCATCC
TGAAATAAAATTTGTGTCTTTAGTTTGTATAGATTTGGCTGGTAATGATACTGACGAAAAAATTCCTATAAAATTATTTT
TAGAAGACATGAGTAAATTCATAAAAGGCTGTGTTCAAACAGACGGATCCTCTGTTGTCCTTCCAGGTATTGCAACTCTT
AATAATGCTAAAGTTGATATGATCGTGGACATGGATGTTAACTGGTTTATAGATTATAACTATGAACTTATAGACTGTAA
AACCGAAAAACCAACCGGTACTCTTAGAATTCCTTGCTTTCTTTACCACGAAAATAAACCCGTGGATTCCAGATATATAT
TAAAAAAATCAATAGAATATTTTAAAACAACTCTATTAAATTTAATAAAGACTAATCCTGAATCTATATCTTCTTTCAAT
ATAAAATATGAAGATATAGAAAAAGTAGATGTAACTTGTGCTACAGAACTAGAATTTTGGGTGAAAACTCCAAATGACAA
TGCAGAAGTAGATGAATTGTCTACCTCCCAAGTTCTTCATGAACAATACTGGAATAGAACTAAAGGAAGCGTTAGAACTG
CTTTAGAGGAATCTTTATTACTTATGGAATATCACGGATTAGAGCCTGAAATGGGTCATAAGGAAGTTGGCGGAGTAAAA
GCTAAATTAACTAGTTCCGGTGGATTTACTCATATAATGGAACAGCTAGAAGTTGACTGGAAATATTCAACGGCCCTTCA
AGCAGGAGATAATGAATTATTAGTAAAAAGCATAATAAAAGAAACCTTTAGAAATAACAAATTAGATGTTACATTTTTTG
CAAAACCTATAGATGAAGTTGCAGGCAGCGGAAAACATGTTCATTTAGGGGTTGCGCTTAAATTAAAAAATGGAAAAAGA
ATCAATCTTTTCTCTACAGATAAAAACCACTACCTAAGTTCTTTTGGTTATGCTTCTATAATGGGCATACTAAAAAATTA
TGAAGTGATAAATCCTTTTGTTTCATCAACTAACGATTCTTTAAGAAGGCTAAAACCAGGCTTTGAAGCACCTGTATGTA
TAGTAACATCTTTAGGCCATACTGTACAATTACCATCAAGAAATAGAACAATCCTAGTTGGGGTTATAAGAGACTTAGAT
AATTCTTTAGCTACAAGATTTGAGTTAAGATCACCAAATCCTCGTAGCAATACTTATCTAACAGTAGCTTCTTTATGTAT
GTCTATGATTGATGGAATAAAATATGCTATAGAAAATAATAAATGTGAAGATGATCTTTTAAGAGAGCTTTCTAAAACTG
CTGAGGAAGATACTAGCTATCTTGAAAAGGGAAGACAATATAGAAGTGAAGAAGACGTATTTGAACACTACAGCGAAGAA
GAAAGAAGTAAAATCTTTGGCACAGCACCTTCTAATGTATATGAAAATATATGCTCTTTAGATAAATATATGGATAAAAT
AGAAGTATTAAAAGCTGGTGAGGTATTCACCTCTAAAATTATTAATAGCTACAAACTAGCTGTTATTGAAAGATGGTTAG
TTGAAATAACAAATAGAATAATTCCAAATTATGTAGAAGAAATCAGAAGCTTTTCTCAATTACATGAAGTAAACAAAGCT
TCTGATTTAGATATTCATAACTGGTCAAAAATCAATGAACTTAGACAATGTCTAATGAAAGATACCTACTCTGATAAATC
TTTATTTGGTCAAATAAGAGAAGCTAAAGAAAATAACGATTATGAAGCTATTTCTAATTTACAATTAGAAATAGATAAAA
AAATGAAATTACTTAGAAAACTTTATTCCGATTACAAAAAAAATCTTTTAGATATATAA

Upstream 100 bases:

>100_bases
GACATATAAAAATATTAATTATAATATATTGCATATATTATATACAATAAAATTATATAATGTTAAATTTATTGTATTTA
ACAATTGGGGGAGGTAAATT

Downstream 100 bases:

>100_bases
AATAAAAGCACACCTAGTATCAGGTGTGCTTTTATCTTATAATATTAAAATTCTTTTAATGGAAAAGACTTTATTACCAT
TTCCTTTTTATTATTTATAG

Product: putative glutamine synthetase

Products: NA

Alternate protein names: GS; Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 632; Mature: 632

Protein sequence:

>632_residues
MLDDLVYVIPKEMHSEEQLKEFLVSHPEIKFVSLVCIDLAGNDTDEKIPIKLFLEDMSKFIKGCVQTDGSSVVLPGIATL
NNAKVDMIVDMDVNWFIDYNYELIDCKTEKPTGTLRIPCFLYHENKPVDSRYILKKSIEYFKTTLLNLIKTNPESISSFN
IKYEDIEKVDVTCATELEFWVKTPNDNAEVDELSTSQVLHEQYWNRTKGSVRTALEESLLLMEYHGLEPEMGHKEVGGVK
AKLTSSGGFTHIMEQLEVDWKYSTALQAGDNELLVKSIIKETFRNNKLDVTFFAKPIDEVAGSGKHVHLGVALKLKNGKR
INLFSTDKNHYLSSFGYASIMGILKNYEVINPFVSSTNDSLRRLKPGFEAPVCIVTSLGHTVQLPSRNRTILVGVIRDLD
NSLATRFELRSPNPRSNTYLTVASLCMSMIDGIKYAIENNKCEDDLLRELSKTAEEDTSYLEKGRQYRSEEDVFEHYSEE
ERSKIFGTAPSNVYENICSLDKYMDKIEVLKAGEVFTSKIINSYKLAVIERWLVEITNRIIPNYVEEIRSFSQLHEVNKA
SDLDIHNWSKINELRQCLMKDTYSDKSLFGQIREAKENNDYEAISNLQLEIDKKMKLLRKLYSDYKKNLLDI

Sequences:

>Translated_632_residues
MLDDLVYVIPKEMHSEEQLKEFLVSHPEIKFVSLVCIDLAGNDTDEKIPIKLFLEDMSKFIKGCVQTDGSSVVLPGIATL
NNAKVDMIVDMDVNWFIDYNYELIDCKTEKPTGTLRIPCFLYHENKPVDSRYILKKSIEYFKTTLLNLIKTNPESISSFN
IKYEDIEKVDVTCATELEFWVKTPNDNAEVDELSTSQVLHEQYWNRTKGSVRTALEESLLLMEYHGLEPEMGHKEVGGVK
AKLTSSGGFTHIMEQLEVDWKYSTALQAGDNELLVKSIIKETFRNNKLDVTFFAKPIDEVAGSGKHVHLGVALKLKNGKR
INLFSTDKNHYLSSFGYASIMGILKNYEVINPFVSSTNDSLRRLKPGFEAPVCIVTSLGHTVQLPSRNRTILVGVIRDLD
NSLATRFELRSPNPRSNTYLTVASLCMSMIDGIKYAIENNKCEDDLLRELSKTAEEDTSYLEKGRQYRSEEDVFEHYSEE
ERSKIFGTAPSNVYENICSLDKYMDKIEVLKAGEVFTSKIINSYKLAVIERWLVEITNRIIPNYVEEIRSFSQLHEVNKA
SDLDIHNWSKINELRQCLMKDTYSDKSLFGQIREAKENNDYEAISNLQLEIDKKMKLLRKLYSDYKKNLLDI
>Mature_632_residues
MLDDLVYVIPKEMHSEEQLKEFLVSHPEIKFVSLVCIDLAGNDTDEKIPIKLFLEDMSKFIKGCVQTDGSSVVLPGIATL
NNAKVDMIVDMDVNWFIDYNYELIDCKTEKPTGTLRIPCFLYHENKPVDSRYILKKSIEYFKTTLLNLIKTNPESISSFN
IKYEDIEKVDVTCATELEFWVKTPNDNAEVDELSTSQVLHEQYWNRTKGSVRTALEESLLLMEYHGLEPEMGHKEVGGVK
AKLTSSGGFTHIMEQLEVDWKYSTALQAGDNELLVKSIIKETFRNNKLDVTFFAKPIDEVAGSGKHVHLGVALKLKNGKR
INLFSTDKNHYLSSFGYASIMGILKNYEVINPFVSSTNDSLRRLKPGFEAPVCIVTSLGHTVQLPSRNRTILVGVIRDLD
NSLATRFELRSPNPRSNTYLTVASLCMSMIDGIKYAIENNKCEDDLLRELSKTAEEDTSYLEKGRQYRSEEDVFEHYSEE
ERSKIFGTAPSNVYENICSLDKYMDKIEVLKAGEVFTSKIINSYKLAVIERWLVEITNRIIPNYVEEIRSFSQLHEVNKA
SDLDIHNWSKINELRQCLMKDTYSDKSLFGQIREAKENNDYEAISNLQLEIDKKMKLLRKLYSDYKKNLLDI

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=527, Percent_Identity=26.7552182163188, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 72464; Mature: 72464

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDDLVYVIPKEMHSEEQLKEFLVSHPEIKFVSLVCIDLAGNDTDEKIPIKLFLEDMSKF
CCCCCEEECCHHCCCHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHH
IKGCVQTDGSSVVLPGIATLNNAKVDMIVDMDVNWFIDYNYELIDCKTEKPTGTLRIPCF
HHHHHCCCCCEEEECCCEECCCCCEEEEEECCCEEEEECCEEEEEEECCCCCCEEEEEEE
LYHENKPVDSRYILKKSIEYFKTTLLNLIKTNPESISSFNIKYEDIEKVDVTCATELEFW
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHCHHHCCEEEEEEEEEE
VKTPNDNAEVDELSTSQVLHEQYWNRTKGSVRTALEESLLLMEYHGLEPEMGHKEVGGVK
EECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCEE
AKLTSSGGFTHIMEQLEVDWKYSTALQAGDNELLVKSIIKETFRNNKLDVTFFAKPIDEV
EEEECCCCHHHHHHHHHCCCEEHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHH
AGSGKHVHLGVALKLKNGKRINLFSTDKNHYLSSFGYASIMGILKNYEVINPFVSSTNDS
CCCCCEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHH
LRRLKPGFEAPVCIVTSLGHTVQLPSRNRTILVGVIRDLDNSLATRFELRSPNPRSNTYL
HHHCCCCCCCCEEEEECCCCEEECCCCCCEEEEEEHHHHHHHHHHEEEECCCCCCCCCCH
TVASLCMSMIDGIKYAIENNKCEDDLLRELSKTAEEDTSYLEKGRQYRSEEDVFEHYSEE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHH
ERSKIFGTAPSNVYENICSLDKYMDKIEVLKAGEVFTSKIINSYKLAVIERWLVEITNRI
HHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPNYVEEIRSFSQLHEVNKASDLDIHNWSKINELRQCLMKDTYSDKSLFGQIREAKENND
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
YEAISNLQLEIDKKMKLLRKLYSDYKKNLLDI
HHHHHCCCEEHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLDDLVYVIPKEMHSEEQLKEFLVSHPEIKFVSLVCIDLAGNDTDEKIPIKLFLEDMSKF
CCCCCEEECCHHCCCHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHH
IKGCVQTDGSSVVLPGIATLNNAKVDMIVDMDVNWFIDYNYELIDCKTEKPTGTLRIPCF
HHHHHCCCCCEEEECCCEECCCCCEEEEEECCCEEEEECCEEEEEEECCCCCCEEEEEEE
LYHENKPVDSRYILKKSIEYFKTTLLNLIKTNPESISSFNIKYEDIEKVDVTCATELEFW
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHCHHHCCEEEEEEEEEE
VKTPNDNAEVDELSTSQVLHEQYWNRTKGSVRTALEESLLLMEYHGLEPEMGHKEVGGVK
EECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCEE
AKLTSSGGFTHIMEQLEVDWKYSTALQAGDNELLVKSIIKETFRNNKLDVTFFAKPIDEV
EEEECCCCHHHHHHHHHCCCEEHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHH
AGSGKHVHLGVALKLKNGKRINLFSTDKNHYLSSFGYASIMGILKNYEVINPFVSSTNDS
CCCCCEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHH
LRRLKPGFEAPVCIVTSLGHTVQLPSRNRTILVGVIRDLDNSLATRFELRSPNPRSNTYL
HHHCCCCCCCCEEEEECCCCEEECCCCCCEEEEEEHHHHHHHHHHEEEECCCCCCCCCCH
TVASLCMSMIDGIKYAIENNKCEDDLLRELSKTAEEDTSYLEKGRQYRSEEDVFEHYSEE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHH
ERSKIFGTAPSNVYENICSLDKYMDKIEVLKAGEVFTSKIINSYKLAVIERWLVEITNRI
HHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPNYVEEIRSFSQLHEVNKASDLDIHNWSKINELRQCLMKDTYSDKSLFGQIREAKENND
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
YEAISNLQLEIDKKMKLLRKLYSDYKKNLLDI
HHHHHCCCEEHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]