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The map label for this gene is mdeA [H]

Identifier: 226949174

GI number: 226949174

Start: 2149619

End: 2150899

Strand: Reverse

Name: mdeA [H]

Synonym: CLM_2092

Alternate gene names: 226949174

Gene position: 2150899-2149619 (Counterclockwise)

Preceding gene: 226949175

Following gene: 226949173

Centisome position: 51.76

GC content: 32.55

Gene sequence:

>1281_bases
ATGAGTATTAAAAATTGGAACACAGAAACCATATGTATTCAAGGAGGCTATACTCCAAAGTCAGGGGAACCAAGAATTCT
TCCTATTATTCAAAGCACCACTTACAAATATGATGACCCAGATAAAGTAGCTAACTTATTTGACCTTAAAGAGGAAGGAC
ATATGTATACAAGAATAAGTAATCCGACAGTGGCTGCTTTCGAAGAAAAAATAGCACAGTTAGAAGGTGGAGTAGGGGCT
GTAGCAGTTTCCTCTGGGCAAAGTGCTACCACATTAGCAGTTTTAAATATTTGTAGTGCAGGAGATCATATACTAGCCTC
CTCAAATTTATATGGAGGTACATTTACCCTTATTTCATCTACTTTAAAGAAATTAGGAATAGAAACTACTTTCGTTTCAC
CGGAAGCATCAGAAGAAGAAATATTAAAACTTGCCAAAGATAATACCAAAATGGTATTAGGAGAAACTATTGGTAACCCC
TCAGTAAATATTTTAGACTTTCATAAGTTTTCAAATGTGGCTAGAAAAATAGAGGTACCTTTTTTAGTAGATAATACATT
AATGACTCCTTATCTTTGTAAGCCCTTTGAATATGGAGCCGATATAGTAATTCATTCCGCTACTAAATATATAGATGGTC
ATGCAACCAGTGTAGGAGGAGTTGTTATAGATGGAGGAAATTTTAACTGGAGAAATGGAAAGTTTCCTTCTTTAGTAGAT
AAAGATCCTACCTATCATGGTATAAGTTACACAGAAGAATTTAAAAATTTAGCTTATATAACTAAATTAAGGGTAAATCT
TTTAAGAGATTTAGGAACTTGTTTAAGTCCCTTTAACGCTTTCTTATTTAATTTAGGATTAGAAACATTACACCTTAGAA
TGGAGAGACATAGTGAAAATGCTTTAAAATTAGCACAATTTTTACAGGAGCATGACAAGATAACTTGGGTAAAGTATCCT
TTATTAAAGGGTGATAAAAGTTATAAAAATGCTAAAAAATATTTAAATAGTGGAGCTAGTGGTATGTTAACTTTCGGTAT
AAAAGGAGGAACAGATCAGGCTAAAGAATTTATTAAAAGTCTAAAATTAGCGAGCTTAGTAATACACATAGGAGATGCAA
GAACTAGTGTACTTCATCCCTCTAGCACAACCCATAGACAACTATCCTATGAAGAACAAATTGCATCTGGGGTAACAGAA
GACTTAATAAGAGTATCTGTAGGTATAGAATACATTGGAGATATAATAAAAGATTTTAAAGAAGCATTGAGTAAAATATA
A

Upstream 100 bases:

>100_bases
TTGTAACTGTTAATAAAGTCATTTCTCACGAAATGGCTTTTTTTAATACAAAAATTTAATTCTAGATTAAAAATATTTTA
AATCATAAGGGGGACAAGAA

Downstream 100 bases:

>100_bases
GTAAAGCGGTTTAAAGCTTTAGATAAAGGAGGTAGAACTATGGTTTCAATTGCTATTTTGGGTAATGGAGTGGTAGGTTC
AGGATTAGTAGAATTAATAG

Product: O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase family protein

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MSIKNWNTETICIQGGYTPKSGEPRILPIIQSTTYKYDDPDKVANLFDLKEEGHMYTRISNPTVAAFEEKIAQLEGGVGA
VAVSSGQSATTLAVLNICSAGDHILASSNLYGGTFTLISSTLKKLGIETTFVSPEASEEEILKLAKDNTKMVLGETIGNP
SVNILDFHKFSNVARKIEVPFLVDNTLMTPYLCKPFEYGADIVIHSATKYIDGHATSVGGVVIDGGNFNWRNGKFPSLVD
KDPTYHGISYTEEFKNLAYITKLRVNLLRDLGTCLSPFNAFLFNLGLETLHLRMERHSENALKLAQFLQEHDKITWVKYP
LLKGDKSYKNAKKYLNSGASGMLTFGIKGGTDQAKEFIKSLKLASLVIHIGDARTSVLHPSSTTHRQLSYEEQIASGVTE
DLIRVSVGIEYIGDIIKDFKEALSKI

Sequences:

>Translated_426_residues
MSIKNWNTETICIQGGYTPKSGEPRILPIIQSTTYKYDDPDKVANLFDLKEEGHMYTRISNPTVAAFEEKIAQLEGGVGA
VAVSSGQSATTLAVLNICSAGDHILASSNLYGGTFTLISSTLKKLGIETTFVSPEASEEEILKLAKDNTKMVLGETIGNP
SVNILDFHKFSNVARKIEVPFLVDNTLMTPYLCKPFEYGADIVIHSATKYIDGHATSVGGVVIDGGNFNWRNGKFPSLVD
KDPTYHGISYTEEFKNLAYITKLRVNLLRDLGTCLSPFNAFLFNLGLETLHLRMERHSENALKLAQFLQEHDKITWVKYP
LLKGDKSYKNAKKYLNSGASGMLTFGIKGGTDQAKEFIKSLKLASLVIHIGDARTSVLHPSSTTHRQLSYEEQIASGVTE
DLIRVSVGIEYIGDIIKDFKEALSKI
>Mature_425_residues
SIKNWNTETICIQGGYTPKSGEPRILPIIQSTTYKYDDPDKVANLFDLKEEGHMYTRISNPTVAAFEEKIAQLEGGVGAV
AVSSGQSATTLAVLNICSAGDHILASSNLYGGTFTLISSTLKKLGIETTFVSPEASEEEILKLAKDNTKMVLGETIGNPS
VNILDFHKFSNVARKIEVPFLVDNTLMTPYLCKPFEYGADIVIHSATKYIDGHATSVGGVVIDGGNFNWRNGKFPSLVDK
DPTYHGISYTEEFKNLAYITKLRVNLLRDLGTCLSPFNAFLFNLGLETLHLRMERHSENALKLAQFLQEHDKITWVKYPL
LKGDKSYKNAKKYLNSGASGMLTFGIKGGTDQAKEFIKSLKLASLVIHIGDARTSVLHPSSTTHRQLSYEEQIASGVTED
LIRVSVGIEYIGDIIKDFKEALSKI

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=403, Percent_Identity=32.0099255583127, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI299473759, Length=403, Percent_Identity=30.0248138957816, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI25453487, Length=401, Percent_Identity=28.927680798005, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI1790375, Length=396, Percent_Identity=30.050505050505, Blast_Score=178, Evalue=5e-46,
Organism=Escherichia coli, GI1789383, Length=377, Percent_Identity=25.1989389920424, Blast_Score=130, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17538055, Length=406, Percent_Identity=34.9753694581281, Blast_Score=215, Evalue=4e-56,
Organism=Caenorhabditis elegans, GI17560056, Length=388, Percent_Identity=35.3092783505155, Blast_Score=212, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI71980708, Length=413, Percent_Identity=32.2033898305085, Blast_Score=208, Evalue=4e-54,
Organism=Saccharomyces cerevisiae, GI6323333, Length=415, Percent_Identity=46.5060240963855, Blast_Score=340, Evalue=3e-94,
Organism=Saccharomyces cerevisiae, GI6319307, Length=406, Percent_Identity=33.9901477832512, Blast_Score=207, Evalue=4e-54,
Organism=Saccharomyces cerevisiae, GI6321254, Length=440, Percent_Identity=27.0454545454545, Blast_Score=123, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6321170, Length=325, Percent_Identity=27.3846153846154, Blast_Score=112, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6321904, Length=409, Percent_Identity=27.1393643031785, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19922554, Length=421, Percent_Identity=33.729216152019, Blast_Score=199, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46928; Mature: 46797

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKNWNTETICIQGGYTPKSGEPRILPIIQSTTYKYDDPDKVANLFDLKEEGHMYTRIS
CCCCCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEC
NPTVAAFEEKIAQLEGGVGAVAVSSGQSATTLAVLNICSAGDHILASSNLYGGTFTLISS
CCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEECCCCCCHHHHHHHH
TLKKLGIETTFVSPEASEEEILKLAKDNTKMVLGETIGNPSVNILDFHKFSNVARKIEVP
HHHHCCCEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCCCEEEEHHHHHHHHHHEECC
FLVDNTLMTPYLCKPFEYGADIVIHSATKYIDGHATSVGGVVIDGGNFNWRNGKFPSLVD
EEECCCCCCCHHCCCCCCCCCEEEECCHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCC
KDPTYHGISYTEEFKNLAYITKLRVNLLRDLGTCLSPFNAFLFNLGLETLHLRMERHSEN
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCH
ALKLAQFLQEHDKITWVKYPLLKGDKSYKNAKKYLNSGASGMLTFGIKGGTDQAKEFIKS
HHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHH
LKLASLVIHIGDARTSVLHPSSTTHRQLSYEEQIASGVTEDLIRVSVGIEYIGDIIKDFK
HHHHHHEEEECCCHHEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
EALSKI
HHHHCC
>Mature Secondary Structure 
SIKNWNTETICIQGGYTPKSGEPRILPIIQSTTYKYDDPDKVANLFDLKEEGHMYTRIS
CCCCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEC
NPTVAAFEEKIAQLEGGVGAVAVSSGQSATTLAVLNICSAGDHILASSNLYGGTFTLISS
CCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEECCCCCCHHHHHHHH
TLKKLGIETTFVSPEASEEEILKLAKDNTKMVLGETIGNPSVNILDFHKFSNVARKIEVP
HHHHCCCEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCCCEEEEHHHHHHHHHHEECC
FLVDNTLMTPYLCKPFEYGADIVIHSATKYIDGHATSVGGVVIDGGNFNWRNGKFPSLVD
EEECCCCCCCHHCCCCCCCCCEEEECCHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCC
KDPTYHGISYTEEFKNLAYITKLRVNLLRDLGTCLSPFNAFLFNLGLETLHLRMERHSEN
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCH
ALKLAQFLQEHDKITWVKYPLLKGDKSYKNAKKYLNSGASGMLTFGIKGGTDQAKEFIKS
HHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHH
LKLASLVIHIGDARTSVLHPSSTTHRQLSYEEQIASGVTEDLIRVSVGIEYIGDIIKDFK
HHHHHHEEEECCCHHEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
EALSKI
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]