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The map label for this gene is hisZ [H]

Identifier: 226948896

GI number: 226948896

Start: 1837543

End: 1838811

Strand: Direct

Name: hisZ [H]

Synonym: CLM_1806

Alternate gene names: 226948896

Gene position: 1837543-1838811 (Clockwise)

Preceding gene: 226948895

Following gene: 226948897

Centisome position: 44.22

GC content: 26.0

Gene sequence:

>1269_bases
ATGGAAAATTGGAATAAGTATATACCGGAAGGTATGAAAGATATTTTGTTTAAAGAGAGCAATATAAAGTTAAATATAGA
GGATCAATTAAGAAAAATTTATAAATATAGTGGATTCTCTGAAATAATATCTCCTACCATAGAATTTTATGATGTATTTA
ATTCTAATATTCAAGCTATACCTCAAGAAAAAATGTATAAACTCTTTGATAATTTAGGTAGAATTCTTGTTTTAAGACCA
GATATGACTACTCCGATAGGGAGAATTACAGGCACTAAAATGAAGGATTGTACATATCCACTTAAACTTTGTTATACAGC
AAATATATTTAGAGTTAATGAAAAATTAAATGGAAAGCGAGGAGAAATAACTCAATCAGGAATTGAAATAATTGGTACTA
ATGGAATAAAATCAGATGTGGATAGTATAGTTACAGCTATAAATGCTCTTTTAAGCGTAGGTCTTAAAAATTTCAAAATA
GAACTAGGAGAGGCTGGATTTTTTCAGGCTCTAACAGAAAACATGGAAATAAAAGAGGAAAATTTAAAAAAATTAAAAGA
AATAATAAGAAATAAGAACTATGTGGCTCTTAAAAAATTTTTAGATGAAATAAGTTCAAAGTATTCAAAGGAGGATTTTG
AACTTATAAAAAATCTGCCTAAATTATTTGGAGATATTAAAATTATAGAAAAGGCTAAGGCTTTAACTAAAAATAAAAAA
GCTTTAAAATCCTTAGATGACATACATAACATATATAAATCCATAGAAAATATAGGACTTGAAGCATATATATCCATTGA
TCTTGGAATGGTTCAAAACATAGATTACTATACAGGGGTTATATTTAAAGGTTATGTAGAAGAAGTAGGAGATTCTATAT
TAAGTGGAGGACGATATGATAATTTAATTCAACATTTTGGAATAGAACTACCTGCTACAGGATTTGCTATTAATGTAGAT
GATATAATGATTGCTTTAAAGAAACAAAATACAATGTCTATGGATAAAGACAAGAAGGTTTTAATATTTTACAAAGAGGA
GTTTTTAAGAAAAGCATATGATTTTATGCAAGAGCTAAAAATGAAAAAAATTATATGTGAATTAAGTCTTTTAGATGATG
ATAAAGAGATTTTATTATACTCAAAGAAGAAAGGTATAGATTTTATTATAGGGTTTATGGGAGAAGAAAAACTATTTGTT
AAGGATTTAAAATCAGATAAAATAGCTTTTCTAAAGAAAGATGAGATTGAAAATTTGTTAATGTTGTAA

Upstream 100 bases:

>100_bases
GAAAAATTGTATTGACAGTAGTAAAAAGAAATGATAATATATTTTTAATTTATCACTTTATCCAAATAAACTAATAAAGC
GGTAAAGCGAGGGAGATAGA

Downstream 100 bases:

>100_bases
TTAATAAGGAAATGGGTGATTACATTGAAAAATGTAAAAATAGCGTTAACCAAAGGTAGACTTGAAAAGAAAGCTATTGA
AATATTTAAAACTATTAATA

Product: ATP phosphoribosyltransferase regulatory subunit

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MENWNKYIPEGMKDILFKESNIKLNIEDQLRKIYKYSGFSEIISPTIEFYDVFNSNIQAIPQEKMYKLFDNLGRILVLRP
DMTTPIGRITGTKMKDCTYPLKLCYTANIFRVNEKLNGKRGEITQSGIEIIGTNGIKSDVDSIVTAINALLSVGLKNFKI
ELGEAGFFQALTENMEIKEENLKKLKEIIRNKNYVALKKFLDEISSKYSKEDFELIKNLPKLFGDIKIIEKAKALTKNKK
ALKSLDDIHNIYKSIENIGLEAYISIDLGMVQNIDYYTGVIFKGYVEEVGDSILSGGRYDNLIQHFGIELPATGFAINVD
DIMIALKKQNTMSMDKDKKVLIFYKEEFLRKAYDFMQELKMKKIICELSLLDDDKEILLYSKKKGIDFIIGFMGEEKLFV
KDLKSDKIAFLKKDEIENLLML

Sequences:

>Translated_422_residues
MENWNKYIPEGMKDILFKESNIKLNIEDQLRKIYKYSGFSEIISPTIEFYDVFNSNIQAIPQEKMYKLFDNLGRILVLRP
DMTTPIGRITGTKMKDCTYPLKLCYTANIFRVNEKLNGKRGEITQSGIEIIGTNGIKSDVDSIVTAINALLSVGLKNFKI
ELGEAGFFQALTENMEIKEENLKKLKEIIRNKNYVALKKFLDEISSKYSKEDFELIKNLPKLFGDIKIIEKAKALTKNKK
ALKSLDDIHNIYKSIENIGLEAYISIDLGMVQNIDYYTGVIFKGYVEEVGDSILSGGRYDNLIQHFGIELPATGFAINVD
DIMIALKKQNTMSMDKDKKVLIFYKEEFLRKAYDFMQELKMKKIICELSLLDDDKEILLYSKKKGIDFIIGFMGEEKLFV
KDLKSDKIAFLKKDEIENLLML
>Mature_422_residues
MENWNKYIPEGMKDILFKESNIKLNIEDQLRKIYKYSGFSEIISPTIEFYDVFNSNIQAIPQEKMYKLFDNLGRILVLRP
DMTTPIGRITGTKMKDCTYPLKLCYTANIFRVNEKLNGKRGEITQSGIEIIGTNGIKSDVDSIVTAINALLSVGLKNFKI
ELGEAGFFQALTENMEIKEENLKKLKEIIRNKNYVALKKFLDEISSKYSKEDFELIKNLPKLFGDIKIIEKAKALTKNKK
ALKSLDDIHNIYKSIENIGLEAYISIDLGMVQNIDYYTGVIFKGYVEEVGDSILSGGRYDNLIQHFGIELPATGFAINVD
DIMIALKKQNTMSMDKDKKVLIFYKEEFLRKAYDFMQELKMKKIICELSLLDDDKEILLYSKKKGIDFIIGFMGEEKLFV
KDLKSDKIAFLKKDEIENLLML

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine [H]

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily [H]

Homologues:

Organism=Homo sapiens, GI15029520, Length=419, Percent_Identity=22.673031026253, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI6996014, Length=346, Percent_Identity=21.9653179190751, Blast_Score=82, Evalue=9e-16,
Organism=Escherichia coli, GI1788861, Length=331, Percent_Identity=25.6797583081571, Blast_Score=84, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6325290, Length=377, Percent_Identity=24.9336870026525, Blast_Score=101, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24643061, Length=391, Percent_Identity=23.0179028132992, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24643059, Length=409, Percent_Identity=22.0048899755501, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004517
- InterPro:   IPR004516 [H]

Pfam domain/function: PF00587 tRNA-synt_2b [H]

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 48581; Mature: 48581

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENWNKYIPEGMKDILFKESNIKLNIEDQLRKIYKYSGFSEIISPTIEFYDVFNSNIQAI
CCCCHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCC
PQEKMYKLFDNLGRILVLRPDMTTPIGRITGTKMKDCTYPLKLCYTANIFRVNEKLNGKR
CHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHEEECEEEECHHCCCCC
GEITQSGIEIIGTNGIKSDVDSIVTAINALLSVGLKNFKIELGEAGFFQALTENMEIKEE
CCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCHH
NLKKLKEIIRNKNYVALKKFLDEISSKYSKEDFELIKNLPKLFGDIKIIEKAKALTKNKK
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
ALKSLDDIHNIYKSIENIGLEAYISIDLGMVQNIDYYTGVIFKGYVEEVGDSILSGGRYD
HHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHH
NLIQHFGIELPATGFAINVDDIMIALKKQNTMSMDKDKKVLIFYKEEFLRKAYDFMQELK
HHHHHCCCCCCCCCEEEEHHHHEEEEECCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHH
MKKIICELSLLDDDKEILLYSKKKGIDFIIGFMGEEKLFVKDLKSDKIAFLKKDEIENLL
HHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCHHHHHHCCCCCEEEEEHHHHHHHH
ML
CC
>Mature Secondary Structure
MENWNKYIPEGMKDILFKESNIKLNIEDQLRKIYKYSGFSEIISPTIEFYDVFNSNIQAI
CCCCHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCC
PQEKMYKLFDNLGRILVLRPDMTTPIGRITGTKMKDCTYPLKLCYTANIFRVNEKLNGKR
CHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHEEECEEEECHHCCCCC
GEITQSGIEIIGTNGIKSDVDSIVTAINALLSVGLKNFKIELGEAGFFQALTENMEIKEE
CCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCHH
NLKKLKEIIRNKNYVALKKFLDEISSKYSKEDFELIKNLPKLFGDIKIIEKAKALTKNKK
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
ALKSLDDIHNIYKSIENIGLEAYISIDLGMVQNIDYYTGVIFKGYVEEVGDSILSGGRYD
HHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHH
NLIQHFGIELPATGFAINVDDIMIALKKQNTMSMDKDKKVLIFYKEEFLRKAYDFMQELK
HHHHHCCCCCCCCCEEEEHHHHEEEEECCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHH
MKKIICELSLLDDDKEILLYSKKKGIDFIIGFMGEEKLFVKDLKSDKIAFLKKDEIENLL
HHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCHHHHHHCCCCCEEEEEHHHHHHHH
ML
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA