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The map label for this gene is cobJ [H]

Identifier: 226948223

GI number: 226948223

Start: 1141062

End: 1141793

Strand: Direct

Name: cobJ [H]

Synonym: CLM_1100

Alternate gene names: 226948223

Gene position: 1141062-1141793 (Clockwise)

Preceding gene: 226948222

Following gene: 226948224

Centisome position: 27.46

GC content: 31.69

Gene sequence:

>732_bases
ATGGGAAAACTTTATGTAGTTGGTATAGGACCAGGAAGTTTAGAGCATATAACAGTAAAAGCTAAAAAGGTATTAGAAGA
AAGTGATATAATAATAGGCTATTCAAAATACATAGATTATGTAAAACCTCTTATAGAAGGAAAAGAAGTACATACTACAG
GAATGAAAAAAGAAGAAGAAAGATGCCAAAGGGCATTAGATCTAAGTAAGGATAAGGTTGTTTCAGTAATAAGTACAGGT
GATGCTGGAATCTATGGTATGGCAGGGCTTATATTAGAAATGAATAAGGATAAGAACTTGGAAATCCAAATAATTCCAGG
GGTAACAGCCTCCACATCAGCAGCTTCTATAATAGGAGCACCACTAATGCATGATAACTGTAATATAAGTTTAAGCGATT
TAATGACACCTTATGAACTTATAAAAAAGAGAGTTAAGTTAGCAGCAGAAGGAGATTTTGTTATATCCTTATACAACCCT
AAAAGTAAAGGAAGACCTTATTATTTAAAAGAATGTATAGATATAATAAAGGAATATAGAAGCAAGGATACCCCTATTGC
AGTAGTAAAAAATGCCCTAAGAGAAGGTGAAGAAATACACCTGTATACTTTAGAAAATTTCACAGATGATTTTGCAGATA
TGTTATCTACAGTGATAATAGGGAACAGTAGTAGTTTTATGAAAGAGGGTAAGTTTATAACTAAAAGGGGTTATAAAGTA
CATAAAGATTAA

Upstream 100 bases:

>100_bases
TAACAAATGTTAATATTCGCAATGAAATAAATCAGATAAAAATAATAGAGGGGAGGATTTTAGTGAGAGGTTGTTAGTTT
AAATCTCACTAAATATAAAC

Downstream 100 bases:

>100_bases
AAATTTTAATTCATCAGCATTAGCATTTTGGTGGGACTAATTCAGATCATTCTTATATGGCAAATTAGCAACAGAGCTAT
TAGCATGTTATAGACATAAA

Product: precorrin-3B C(17)-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 4

Alternate protein names: Cobalt-precorrin-3 methylase; Cobalt-precorrin-3 methyltransferase [H]

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MGKLYVVGIGPGSLEHITVKAKKVLEESDIIIGYSKYIDYVKPLIEGKEVHTTGMKKEEERCQRALDLSKDKVVSVISTG
DAGIYGMAGLILEMNKDKNLEIQIIPGVTASTSAASIIGAPLMHDNCNISLSDLMTPYELIKKRVKLAAEGDFVISLYNP
KSKGRPYYLKECIDIIKEYRSKDTPIAVVKNALREGEEIHLYTLENFTDDFADMLSTVIIGNSSSFMKEGKFITKRGYKV
HKD

Sequences:

>Translated_243_residues
MGKLYVVGIGPGSLEHITVKAKKVLEESDIIIGYSKYIDYVKPLIEGKEVHTTGMKKEEERCQRALDLSKDKVVSVISTG
DAGIYGMAGLILEMNKDKNLEIQIIPGVTASTSAASIIGAPLMHDNCNISLSDLMTPYELIKKRVKLAAEGDFVISLYNP
KSKGRPYYLKECIDIIKEYRSKDTPIAVVKNALREGEEIHLYTLENFTDDFADMLSTVIIGNSSSFMKEGKFITKRGYKV
HKD
>Mature_242_residues
GKLYVVGIGPGSLEHITVKAKKVLEESDIIIGYSKYIDYVKPLIEGKEVHTTGMKKEEERCQRALDLSKDKVVSVISTGD
AGIYGMAGLILEMNKDKNLEIQIIPGVTASTSAASIIGAPLMHDNCNISLSDLMTPYELIKKRVKLAAEGDFVISLYNPK
SKGRPYYLKECIDIIKEYRSKDTPIAVVKNALREGEEIHLYTLENFTDDFADMLSTVIIGNSSSFMKEGKFITKRGYKVH
KD

Specific function: Methyltransferase that catalyzes the methylation of C-17 in cobalt-precorrin-3B to form cobalt-precorrin-4 [H]

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006363 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.131

Molecular weight: Translated: 27017; Mature: 26886

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKLYVVGIGPGSLEHITVKAKKVLEESDIIIGYSKYIDYVKPLIEGKEVHTTGMKKEEE
CCEEEEEEECCCCCEEEEHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCEEECCCCCCHHH
RCQRALDLSKDKVVSVISTGDAGIYGMAGLILEMNKDKNLEIQIIPGVTASTSAASIIGA
HHHHHHCCCCCCEEEEEECCCCCHHHHEEEEEEECCCCCEEEEEECCCCCCCCHHHHHCC
PLMHDNCNISLSDLMTPYELIKKRVKLAAEGDFVISLYNPKSKGRPYYLKECIDIIKEYR
CEECCCCCEEHHHHCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHC
SKDTPIAVVKNALREGEEIHLYTLENFTDDFADMLSTVIIGNSSSFMKEGKFITKRGYKV
CCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHEECCCHHHHHCCCEEECCCEEE
HKD
CCC
>Mature Secondary Structure 
GKLYVVGIGPGSLEHITVKAKKVLEESDIIIGYSKYIDYVKPLIEGKEVHTTGMKKEEE
CEEEEEEECCCCCEEEEHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCEEECCCCCCHHH
RCQRALDLSKDKVVSVISTGDAGIYGMAGLILEMNKDKNLEIQIIPGVTASTSAASIIGA
HHHHHHCCCCCCEEEEEECCCCCHHHHEEEEEEECCCCCEEEEEECCCCCCCCHHHHHCC
PLMHDNCNISLSDLMTPYELIKKRVKLAAEGDFVISLYNPKSKGRPYYLKECIDIIKEYR
CEECCCCCEEHHHHCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHC
SKDTPIAVVKNALREGEEIHLYTLENFTDDFADMLSTVIIGNSSSFMKEGKFITKRGYKV
CCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHEECCCHHHHHCCCEEECCCEEE
HKD
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 3B

Specific reaction: S-adenosyl-L-methionine + precorrin-3B = S-adenosyl-L-homocysteine + precorrin-4

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]