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The map label for this gene is lpdA [H]

Identifier: 226947960

GI number: 226947960

Start: 882106

End: 883494

Strand: Direct

Name: lpdA [H]

Synonym: CLM_0819

Alternate gene names: 226947960

Gene position: 882106-883494 (Clockwise)

Preceding gene: 226947959

Following gene: 226947961

Centisome position: 21.23

GC content: 31.32

Gene sequence:

>1389_bases
TTGAAGGATATTATAGTTATAGGTGGAGGTCCTGGTGGATATGTAGCGGCCATAAGAGCTGCACATTTAGGTGCTGATGT
AGCAGTTGTCGAGATAGATAGCTTTGGTGGAACATGCCTTAATAGAGGCTGCATACCAACAAAGACTTTATATAGAACAG
CAGAAATAATGAATATATTAAAACATATAGAAGACTTTGGAATAGAGGCTGAAAACTATAATCTAAATGTAGAGAAGGTT
CAGGAAAGAAAAAATAATGTAATAAAAGAATTAGTAGGTGGAGTTGAGAAATTACTAAAAGGAAATAATGTTGAAATTAT
AAAAGGTAAGGCTTTCTTAAAAGATAAAAATACTGTTTTAGTAGAAACGAAAGATGGGCAAGTTATATTAGAAGGAAAAA
ACATAATTATAGCAACGGGCTCAAATGCTGAAATGCCAGATATAAAAGGCATAGAGAATAAAAAAATAATAATAAGTGAT
GATATTTTAGAATTTGATAGAATTCCTAAACATTTAGTAGTTTCAGGTGGCGGTGTTGTTGGAATGGAATTTGCTAATAT
CTTTAAAGCTATGGGAAGTGAAGTAACAGTAATAGTCGCTAGGGATTCTATATTATATGATATTGATAGAGAAATAAGTA
AAAGGTATAAAGTTATAGCGAAAAAGTCAGGAATAAATATATTAACATCAACTAAAATATTGGAGTTTGCTGAGGATGAT
AATAATGTAACTATAAAATGTGAAAGTAAAAAGGGTGAGTTTGAGTTAAATTCTGATATGGTTCTTATGGCTAAAGGTAG
AAGAGGAAACTTTACAGGTATGAACTTAGAAGAACTAGGGATCGAACATGATAAAAAGAAAATAATTGTAGATGATAATT
ATAAAACTAATATAGATGGAATATACGCAATTGGAGATGTAAATGGAATTTGTCTTTTAGCTCATGCAGCATCACATCAA
GGAATAGAGGTTGTAGAGCATATAATGGAAAATAAAGAATGCCATAAATCTGTGATTCCAAACTGTATATTTACATTCCC
TGAAATAGCCACTGCGGGCATGACGGAGGAAGAAATTAAGGAAAAAGGTATAGAGTATATAAAAAATAAATTCCTATTTG
GAGCAAATGGAAAAGCCTTGGCATTAGGTGAAGGTGAAGGTGTTGTAAAAGTTATATGTGAAAAGGAAAGTAAAAAGATT
TTAGGTATTCATATAATGGGTCCACATGCTTCAGATTTAATACATGAAGGTGTGGTAGCAATTGAAAAGGGTATGACAGT
AAATGATTTTAAAGAAGTAGTACATGCTCACCCAACATTAGGAGAAGCATTCTATGAGGCAATAATGGGATTAAATAAGG
AAGCTATTCATAGTATAAATAAAAAATAA

Upstream 100 bases:

>100_bases
GAGAGGTTCAGAAGAGTTAAGATTTAAGAAAAGTAAAAATACAATTCTTATTTCTTAGCTCTTAAATTTTAAAATTGATT
AAATCATAGGGGGGATAATT

Downstream 100 bases:

>100_bases
AAATCTTAATATTTAATAAGTTGAAATCTAATAAAAAAATCCTATAATGTGCAACTAACATTAATATGAACAAAGTCAGA
GAAGTATTGACTTACTATTA

Product: dihydrolipoyl dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MKDIIVIGGGPGGYVAAIRAAHLGADVAVVEIDSFGGTCLNRGCIPTKTLYRTAEIMNILKHIEDFGIEAENYNLNVEKV
QERKNNVIKELVGGVEKLLKGNNVEIIKGKAFLKDKNTVLVETKDGQVILEGKNIIIATGSNAEMPDIKGIENKKIIISD
DILEFDRIPKHLVVSGGGVVGMEFANIFKAMGSEVTVIVARDSILYDIDREISKRYKVIAKKSGINILTSTKILEFAEDD
NNVTIKCESKKGEFELNSDMVLMAKGRRGNFTGMNLEELGIEHDKKKIIVDDNYKTNIDGIYAIGDVNGICLLAHAASHQ
GIEVVEHIMENKECHKSVIPNCIFTFPEIATAGMTEEEIKEKGIEYIKNKFLFGANGKALALGEGEGVVKVICEKESKKI
LGIHIMGPHASDLIHEGVVAIEKGMTVNDFKEVVHAHPTLGEAFYEAIMGLNKEAIHSINKK

Sequences:

>Translated_462_residues
MKDIIVIGGGPGGYVAAIRAAHLGADVAVVEIDSFGGTCLNRGCIPTKTLYRTAEIMNILKHIEDFGIEAENYNLNVEKV
QERKNNVIKELVGGVEKLLKGNNVEIIKGKAFLKDKNTVLVETKDGQVILEGKNIIIATGSNAEMPDIKGIENKKIIISD
DILEFDRIPKHLVVSGGGVVGMEFANIFKAMGSEVTVIVARDSILYDIDREISKRYKVIAKKSGINILTSTKILEFAEDD
NNVTIKCESKKGEFELNSDMVLMAKGRRGNFTGMNLEELGIEHDKKKIIVDDNYKTNIDGIYAIGDVNGICLLAHAASHQ
GIEVVEHIMENKECHKSVIPNCIFTFPEIATAGMTEEEIKEKGIEYIKNKFLFGANGKALALGEGEGVVKVICEKESKKI
LGIHIMGPHASDLIHEGVVAIEKGMTVNDFKEVVHAHPTLGEAFYEAIMGLNKEAIHSINKK
>Mature_462_residues
MKDIIVIGGGPGGYVAAIRAAHLGADVAVVEIDSFGGTCLNRGCIPTKTLYRTAEIMNILKHIEDFGIEAENYNLNVEKV
QERKNNVIKELVGGVEKLLKGNNVEIIKGKAFLKDKNTVLVETKDGQVILEGKNIIIATGSNAEMPDIKGIENKKIIISD
DILEFDRIPKHLVVSGGGVVGMEFANIFKAMGSEVTVIVARDSILYDIDREISKRYKVIAKKSGINILTSTKILEFAEDD
NNVTIKCESKKGEFELNSDMVLMAKGRRGNFTGMNLEELGIEHDKKKIIVDDNYKTNIDGIYAIGDVNGICLLAHAASHQ
GIEVVEHIMENKECHKSVIPNCIFTFPEIATAGMTEEEIKEKGIEYIKNKFLFGANGKALALGEGEGVVKVICEKESKKI
LGIHIMGPHASDLIHEGVVAIEKGMTVNDFKEVVHAHPTLGEAFYEAIMGLNKEAIHSINKK

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=458, Percent_Identity=38.646288209607, Blast_Score=291, Evalue=1e-78,
Organism=Homo sapiens, GI50301238, Length=455, Percent_Identity=32.0879120879121, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI22035672, Length=454, Percent_Identity=28.1938325991189, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI291045266, Length=457, Percent_Identity=26.2582056892779, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI33519430, Length=458, Percent_Identity=25.764192139738, Blast_Score=118, Evalue=2e-26,
Organism=Homo sapiens, GI33519428, Length=458, Percent_Identity=25.764192139738, Blast_Score=118, Evalue=2e-26,
Organism=Homo sapiens, GI33519426, Length=458, Percent_Identity=25.764192139738, Blast_Score=118, Evalue=2e-26,
Organism=Homo sapiens, GI148277071, Length=458, Percent_Identity=25.764192139738, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI148277065, Length=458, Percent_Identity=25.764192139738, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI291045268, Length=451, Percent_Identity=25.2771618625277, Blast_Score=112, Evalue=8e-25,
Organism=Escherichia coli, GI1786307, Length=446, Percent_Identity=35.8744394618834, Blast_Score=265, Evalue=7e-72,
Organism=Escherichia coli, GI87082354, Length=467, Percent_Identity=31.0492505353319, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1789915, Length=434, Percent_Identity=30.8755760368664, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI87081717, Length=448, Percent_Identity=27.6785714285714, Blast_Score=163, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI32565766, Length=456, Percent_Identity=37.9385964912281, Blast_Score=297, Evalue=8e-81,
Organism=Caenorhabditis elegans, GI17557007, Length=475, Percent_Identity=28.6315789473684, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI71983429, Length=451, Percent_Identity=28.3813747228381, Blast_Score=154, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI71983419, Length=451, Percent_Identity=28.3813747228381, Blast_Score=154, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI71982272, Length=483, Percent_Identity=26.2939958592132, Blast_Score=109, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6321091, Length=475, Percent_Identity=38.3157894736842, Blast_Score=286, Evalue=6e-78,
Organism=Saccharomyces cerevisiae, GI6325240, Length=469, Percent_Identity=30.2771855010661, Blast_Score=206, Evalue=7e-54,
Organism=Saccharomyces cerevisiae, GI6325166, Length=458, Percent_Identity=29.2576419213974, Blast_Score=171, Evalue=2e-43,
Organism=Drosophila melanogaster, GI21358499, Length=460, Percent_Identity=38.9130434782609, Blast_Score=295, Evalue=7e-80,
Organism=Drosophila melanogaster, GI24640549, Length=476, Percent_Identity=29.2016806722689, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24640553, Length=476, Percent_Identity=29.2016806722689, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24640551, Length=476, Percent_Identity=29.2016806722689, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI17737741, Length=479, Percent_Identity=27.7661795407098, Blast_Score=148, Evalue=6e-36,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50552; Mature: 50552

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDIIVIGGGPGGYVAAIRAAHLGADVAVVEIDSFGGTCLNRGCIPTKTLYRTAEIMNIL
CCCEEEEECCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHCCCCCCHHHHHHHHHHHHHH
KHIEDFGIEAENYNLNVEKVQERKNNVIKELVGGVEKLLKGNNVEIIKGKAFLKDKNTVL
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEECCCEEE
VETKDGQVILEGKNIIIATGSNAEMPDIKGIENKKIIISDDILEFDRIPKHLVVSGGGVV
EEECCCEEEEECCEEEEEECCCCCCCCCCCCCCCEEEEECCHHHHHCCCCEEEEECCCEE
GMEFANIFKAMGSEVTVIVARDSILYDIDREISKRYKVIAKKSGINILTSTKILEFAEDD
HHHHHHHHHHCCCCEEEEEECCCEEEHHHHHHHHHHHHHEECCCCEEEEECEEEEEECCC
NNVTIKCESKKGEFELNSDMVLMAKGRRGNFTGMNLEELGIEHDKKKIIVDDNYKTNIDG
CCEEEEECCCCCCEEECCCEEEEEECCCCCCCCCCHHHHCCCCCCCEEEECCCCCCCCCE
IYAIGDVNGICLLAHAASHQGIEVVEHIMENKECHKSVIPNCIFTFPEIATAGMTEEEIK
EEEEECCCCEEEEEECCCCCCHHHHHHHHCCHHHHHHHCCHHHHCCHHHHHCCCCHHHHH
EKGIEYIKNKFLFGANGKALALGEGEGVVKVICEKESKKILGIHIMGPHASDLIHEGVVA
HHHHHHHHHCEEECCCCCEEEEECCCCEEEEEEECCCCEEEEEEEECCCHHHHHHHCHHH
IEKGMTVNDFKEVVHAHPTLGEAFYEAIMGLNKEAIHSINKK
HCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHCCCC
>Mature Secondary Structure
MKDIIVIGGGPGGYVAAIRAAHLGADVAVVEIDSFGGTCLNRGCIPTKTLYRTAEIMNIL
CCCEEEEECCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHCCCCCCHHHHHHHHHHHHHH
KHIEDFGIEAENYNLNVEKVQERKNNVIKELVGGVEKLLKGNNVEIIKGKAFLKDKNTVL
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEECCCEEE
VETKDGQVILEGKNIIIATGSNAEMPDIKGIENKKIIISDDILEFDRIPKHLVVSGGGVV
EEECCCEEEEECCEEEEEECCCCCCCCCCCCCCCEEEEECCHHHHHCCCCEEEEECCCEE
GMEFANIFKAMGSEVTVIVARDSILYDIDREISKRYKVIAKKSGINILTSTKILEFAEDD
HHHHHHHHHHCCCCEEEEEECCCEEEHHHHHHHHHHHHHEECCCCEEEEECEEEEEECCC
NNVTIKCESKKGEFELNSDMVLMAKGRRGNFTGMNLEELGIEHDKKKIIVDDNYKTNIDG
CCEEEEECCCCCCEEECCCEEEEEECCCCCCCCCCHHHHCCCCCCCEEEECCCCCCCCCE
IYAIGDVNGICLLAHAASHQGIEVVEHIMENKECHKSVIPNCIFTFPEIATAGMTEEEIK
EEEEECCCCEEEEEECCCCCCHHHHHHHHCCHHHHHHHCCHHHHCCHHHHHCCCCHHHHH
EKGIEYIKNKFLFGANGKALALGEGEGVVKVICEKESKKILGIHIMGPHASDLIHEGVVA
HHHHHHHHHCEEECCCCCEEEEECCCCEEEEEEECCCCEEEEEEEECCCHHHHHHHCHHH
IEKGMTVNDFKEVVHAHPTLGEAFYEAIMGLNKEAIHSINKK
HCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2200674; 2253629; 6896188; 8805537 [H]