Click here to switch to the map view.

The map label for this gene is lplA [H]

Identifier: 226947961

GI number: 226947961

Start: 883643

End: 884620

Strand: Direct

Name: lplA [H]

Synonym: CLM_0820

Alternate gene names: 226947961

Gene position: 883643-884620 (Clockwise)

Preceding gene: 226947960

Following gene: 226947962

Centisome position: 21.27

GC content: 28.53

Gene sequence:

>978_bases
ATGAAATATATTGATAATTCAAATACTGATCCATATTTTAATTTAGCAGCTGAAGAGTATTTTTTAAGACATAAAGATGA
TGAATACTTCATTTTATGGAGAGACGAGCCCTGCGTTATAGTTGGAAAGAATCAAAATACATTATCAGAAATAGATATGG
ATTATATAGAAACTAATAAGGTTAAAGTAGTAAGAAGACAAACAGGCGGCGGCTCTGTATTTCATGATTTAGGAAACTTA
AACTATACTTTCATAGTTAAAGATGACGGGAAAAGTTTTAATGATTTTGAAAGATTCTGCAAACCTATAATAGGAGTTTT
GGACACATTAGGTGTTAAAGCTGAATTCGCAGGACGAAATGATTTGTTGATAGATGGTAAAAAGATTTCTGGAACAGCTC
AATGTAAGTACAAAGATAGAGTGATGCATCATGGAACTCTTTTGTTTTCATCAGATATAGTAAATTTAAGCGGTGCTCTA
AAACCTAAAAAGATAAAATTTCAAGACAAAGCGGTTAAGTCAGTTATAAGCAGGATAACTAATATTTCCGAACATTTAAA
TTCAGAAATTGATGTTTTAGCCTTTAAAAATAAGATATTTGATTATATTTTAAAAAGTGAAAAAGATGCAAAAGTTATGC
CATTAGCTCAAGATGAAATAGATGAAATAGAGAAAATTAAAAAAGATAAATATGAAACTTGGGCTTGGAACTTTGGAAGT
TCTCCTAAGTATGATTTTTACAATGAAGGAAAGTTTACAGGTGGAACTATAGAGCTAAATCTAAAAGTAGAAAAAGGTGT
AATTAAAGATATAAAAATATTTGGAGATTTCTTTGGTGTTAAGGATGTAAAAGAAATAGAAGATTTATTAAAGGGTATCG
AACACAAAAAAGGAAAAATAGAGAGTATAATAAAAAATATTAACATAGGTGAATATTTTGCTAGAATAACTGTGGAAGAA
TTTTCAAGCTTATTCTAA

Upstream 100 bases:

>100_bases
GTGCAACTAACATTAATATGAACAAAGTCAGAGAAGTATTGACTTACTATTATGTTGTTTTAAAGGTTAAAGGATAGCAA
GATAGATGGGAGGAAAAATT

Downstream 100 bases:

>100_bases
TATAATAAAAAGGTATAAAAGAATCTATTTTTAAAGTGGTTAGCGTTGTTAACTATTTGAAAATGGATTCTTTTTTGCAT
TTAATAGTTAGAAAAGTTTA

Product: putative lipoate-protein ligase A

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MKYIDNSNTDPYFNLAAEEYFLRHKDDEYFILWRDEPCVIVGKNQNTLSEIDMDYIETNKVKVVRRQTGGGSVFHDLGNL
NYTFIVKDDGKSFNDFERFCKPIIGVLDTLGVKAEFAGRNDLLIDGKKISGTAQCKYKDRVMHHGTLLFSSDIVNLSGAL
KPKKIKFQDKAVKSVISRITNISEHLNSEIDVLAFKNKIFDYILKSEKDAKVMPLAQDEIDEIEKIKKDKYETWAWNFGS
SPKYDFYNEGKFTGGTIELNLKVEKGVIKDIKIFGDFFGVKDVKEIEDLLKGIEHKKGKIESIIKNINIGEYFARITVEE
FSSLF

Sequences:

>Translated_325_residues
MKYIDNSNTDPYFNLAAEEYFLRHKDDEYFILWRDEPCVIVGKNQNTLSEIDMDYIETNKVKVVRRQTGGGSVFHDLGNL
NYTFIVKDDGKSFNDFERFCKPIIGVLDTLGVKAEFAGRNDLLIDGKKISGTAQCKYKDRVMHHGTLLFSSDIVNLSGAL
KPKKIKFQDKAVKSVISRITNISEHLNSEIDVLAFKNKIFDYILKSEKDAKVMPLAQDEIDEIEKIKKDKYETWAWNFGS
SPKYDFYNEGKFTGGTIELNLKVEKGVIKDIKIFGDFFGVKDVKEIEDLLKGIEHKKGKIESIIKNINIGEYFARITVEE
FSSLF
>Mature_325_residues
MKYIDNSNTDPYFNLAAEEYFLRHKDDEYFILWRDEPCVIVGKNQNTLSEIDMDYIETNKVKVVRRQTGGGSVFHDLGNL
NYTFIVKDDGKSFNDFERFCKPIIGVLDTLGVKAEFAGRNDLLIDGKKISGTAQCKYKDRVMHHGTLLFSSDIVNLSGAL
KPKKIKFQDKAVKSVISRITNISEHLNSEIDVLAFKNKIFDYILKSEKDAKVMPLAQDEIDEIEKIKKDKYETWAWNFGS
SPKYDFYNEGKFTGGTIELNLKVEKGVIKDIKIFGDFFGVKDVKEIEDLLKGIEHKKGKIESIIKNINIGEYFARITVEE
FSSLF

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=244, Percent_Identity=30.7377049180328, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI21729884, Length=244, Percent_Identity=30.7377049180328, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI21729882, Length=244, Percent_Identity=30.7377049180328, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI21729880, Length=244, Percent_Identity=30.7377049180328, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI21729878, Length=244, Percent_Identity=30.7377049180328, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1790846, Length=310, Percent_Identity=31.9354838709677, Blast_Score=173, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI71981909, Length=255, Percent_Identity=30.1960784313725, Blast_Score=111, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6322415, Length=284, Percent_Identity=30.9859154929577, Blast_Score=117, Evalue=3e-27,
Organism=Drosophila melanogaster, GI28573464, Length=295, Percent_Identity=27.1186440677966, Blast_Score=121, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 37299; Mature: 37299

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYIDNSNTDPYFNLAAEEYFLRHKDDEYFILWRDEPCVIVGKNQNTLSEIDMDYIETNK
CCCCCCCCCCCEEEHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCHHHHCHHHHCCCC
VKVVRRQTGGGSVFHDLGNLNYTFIVKDDGKSFNDFERFCKPIIGVLDTLGVKAEFAGRN
EEEEEEECCCCHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCC
DLLIDGKKISGTAQCKYKDRVMHHGTLLFSSDIVNLSGALKPKKIKFQDKAVKSVISRIT
CEEEECCCCCCEEEEEHHHHHHHCCEEEEECCCEECCCCCCCCEEEHHHHHHHHHHHHHH
NISEHLNSEIDVLAFKNKIFDYILKSEKDAKVMPLAQDEIDEIEKIKKDKYETWAWNFGS
HHHHHHCCCEEEEHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHCCCCEEEEECCC
SPKYDFYNEGKFTGGTIELNLKVEKGVIKDIKIFGDFFGVKDVKEIEDLLKGIEHKKGKI
CCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHH
ESIIKNINIGEYFARITVEEFSSLF
HHHHHHCCHHHHHHHEEHHHHHCCC
>Mature Secondary Structure
MKYIDNSNTDPYFNLAAEEYFLRHKDDEYFILWRDEPCVIVGKNQNTLSEIDMDYIETNK
CCCCCCCCCCCEEEHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCHHHHCHHHHCCCC
VKVVRRQTGGGSVFHDLGNLNYTFIVKDDGKSFNDFERFCKPIIGVLDTLGVKAEFAGRN
EEEEEEECCCCHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCC
DLLIDGKKISGTAQCKYKDRVMHHGTLLFSSDIVNLSGALKPKKIKFQDKAVKSVISRIT
CEEEECCCCCCEEEEEHHHHHHHCCEEEEECCCEECCCCCCCCEEEHHHHHHHHHHHHHH
NISEHLNSEIDVLAFKNKIFDYILKSEKDAKVMPLAQDEIDEIEKIKKDKYETWAWNFGS
HHHHHHCCCEEEEHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHCCCCEEEEECCC
SPKYDFYNEGKFTGGTIELNLKVEKGVIKDIKIFGDFFGVKDVKEIEDLLKGIEHKKGKI
CCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHH
ESIIKNINIGEYFARITVEEFSSLF
HHHHHHCCHHHHHHHEEHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]