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The map label for this gene is cspR [H]

Identifier: 226947438

GI number: 226947438

Start: 251783

End: 252250

Strand: Direct

Name: cspR [H]

Synonym: CLM_0267

Alternate gene names: 226947438

Gene position: 251783-252250 (Clockwise)

Preceding gene: 226947437

Following gene: 226947439

Centisome position: 6.06

GC content: 29.7

Gene sequence:

>468_bases
TTGAGTTTAAATATAGTATTATTTCAACCAGAGATACCACAAAATACTGGTAATATAGCAAGAACATGTGTGCTTACAAA
TTCTAGATTGCATCTAATAAAACCTTTAGGATTTAGTTTAGATGAAAAGCACTTAAGGAGAGCAGGATTAGATTATTGGC
AATATTTAGATTTATCCATATATGAATCCTATGAGGAGTTAAGAGAGAAATACAAAGATTCAAATTTTTATTTTTCAACT
ACTCATGGTAATAATTTTTATACAGATGTTAAATATAGCGATGGAGATTTCATTGTTTTTGGAAGAGAATCTTCAGGACT
ACCAGATGAAATAAGAGATAGTGATCCTAGTAAGTGTATAAGAGTTCCAATGATTAATACAACTACTAGGTCTTTAAATT
TGTCTAATACTGTAGCTGTAGTTACCTATGAAGCTTTAAGACAAGTTGGTTTCCCAGATATGAAATAG

Upstream 100 bases:

>100_bases
ATTCTTTACTAAAGCTTTATAATAGAGTAATATAGTTATTGTAATAACCCTTGTATACTTATTAATTTAAATATATATTA
TAAAAATGGGAGGAAATATT

Downstream 100 bases:

>100_bases
GAGGAGCTTAACATGGAGAAGATAAAAATTATAACAGATAGCACTGCTGATTTACCTAAGAACATAATAGAAAAATATGA
TATAGAGGTAATACCTTTAT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 155; Mature: 154

Protein sequence:

>155_residues
MSLNIVLFQPEIPQNTGNIARTCVLTNSRLHLIKPLGFSLDEKHLRRAGLDYWQYLDLSIYESYEELREKYKDSNFYFST
THGNNFYTDVKYSDGDFIVFGRESSGLPDEIRDSDPSKCIRVPMINTTTRSLNLSNTVAVVTYEALRQVGFPDMK

Sequences:

>Translated_155_residues
MSLNIVLFQPEIPQNTGNIARTCVLTNSRLHLIKPLGFSLDEKHLRRAGLDYWQYLDLSIYESYEELREKYKDSNFYFST
THGNNFYTDVKYSDGDFIVFGRESSGLPDEIRDSDPSKCIRVPMINTTTRSLNLSNTVAVVTYEALRQVGFPDMK
>Mature_154_residues
SLNIVLFQPEIPQNTGNIARTCVLTNSRLHLIKPLGFSLDEKHLRRAGLDYWQYLDLSIYESYEELREKYKDSNFYFSTT
HGNNFYTDVKYSDGDFIVFGRESSGLPDEIRDSDPSKCIRVPMINTTTRSLNLSNTVAVVTYEALRQVGFPDMK

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=151, Percent_Identity=48.3443708609272, Blast_Score=151, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 17875; Mature: 17744

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLNIVLFQPEIPQNTGNIARTCVLTNSRLHLIKPLGFSLDEKHLRRAGLDYWQYLDLSI
CCEEEEEECCCCCCCCCCEEEEEEEECCCEEEEECCCCCCCHHHHHHCCCCHHHHHHHHH
YESYEELREKYKDSNFYFSTTHGNNFYTDVKYSDGDFIVFGRESSGLPDEIRDSDPSKCI
HHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCEEEEECCCCCCCHHHCCCCCCCEE
RVPMINTTTRSLNLSNTVAVVTYEALRQVGFPDMK
EECEECCCCCEEECCCEEEEEEHHHHHHCCCCCCC
>Mature Secondary Structure 
SLNIVLFQPEIPQNTGNIARTCVLTNSRLHLIKPLGFSLDEKHLRRAGLDYWQYLDLSI
CEEEEEECCCCCCCCCCEEEEEEEECCCEEEEECCCCCCCHHHHHHCCCCHHHHHHHHH
YESYEELREKYKDSNFYFSTTHGNNFYTDVKYSDGDFIVFGRESSGLPDEIRDSDPSKCI
HHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCEEEEECCCCCCCHHHCCCCCCCEE
RVPMINTTTRSLNLSNTVAVVTYEALRQVGFPDMK
EECEECCCCCEEECCCEEEEEEHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]