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The map label for this gene is splB [H]

Identifier: 226947437

GI number: 226947437

Start: 250648

End: 251655

Strand: Direct

Name: splB [H]

Synonym: CLM_0266

Alternate gene names: 226947437

Gene position: 250648-251655 (Clockwise)

Preceding gene: 226947436

Following gene: 226947438

Centisome position: 6.03

GC content: 28.27

Gene sequence:

>1008_bases
ATGTTTATTCCCAAAACAGTTATATTTGAGGAAAAAGCTTTAGAATATGTTAAAGGTAAAGAAATTTTAAAAGAATTAAA
AAATAAAAATATAGAAATAAAATATTCCAAGACAGGAAGAGTAACGGGGCAGAGAGATAAAACTCCAAATGAAATGTATT
CTGAAGGTAAAGATACATTAGTAATAGGCATAAGAAAGTCTTTAGATTTTCAAAGCTGTAAGCCATCAGCTCATTACCAA
TTGCCTTTAGTAAGCGGATGTATGGGAATGTGTGAATATTGTTATTTAAATACTCAAATGGGAAAAAGACCTTACACAAA
AGTGTATGTAAATACAGAAGAGATATTAAATAAGGCTGAAAAATATTCAAGAGAAAGATTACCTGAAATTACAGTTTTTG
AAGGAGCGGCTACATCAGATCCTATACCAGTAGAACCGTATACACATTCCTTAAAGGATACTATAGAATTCTTTGGGCAA
AAACATAATATGAAATTTAGATTTGTAACTAAATTTACAGATGTAAATAGCTTAGTTCATTTAAATCATAACAGAAATAC
TACCATTAGATTTAGTATAAATACAGATAATATAATAAGAAATTTTGAACATAGAACACCCCTGGTTATAGATAGGTTGA
AGGCAGCCGAAAAGATAGCTAAAGCTGGGTATAGTTTAGGATTTATTATAGCACCAGTGTTTATATATAGGGGTTGGGAA
AAAGAGTATTTAGATTTATTAAAAAACATAAATATTATGTTTAAAGGTGTTCCCGTAGAATTTGAAGTTATATCCCATAG
ATTTACCAAAAGGGCTAAGGATAACATATTAAGTGTGTTTTCAAACACAACTTTGCCTATGGTAGAAGAGAACAGAAAAT
TTAAATATGGTCAGTTTGGATATGGAAAATATATATATAAAGATGAAGAGTTACAGAATATAAAAAAATTTTTTAGAGAT
AGTATAACAAAATTTTTTGGAGAAGATAACATTAACTACATTATATAG

Upstream 100 bases:

>100_bases
TGAAATATTTTCTTTTACCATTTTATTCATGTAGATATTATATAATATTATTACATATACTTTAGAAATAAATTGCATAC
TATAACTAAGGAGTGATAGT

Downstream 100 bases:

>100_bases
AGAATTAACATTTTGTTAATTCTTTATATTCTTTACTAAAGCTTTATAATAGAGTAATATAGTTATTGTAATAACCCTTG
TATACTTATTAATTTAAATA

Product: spore photoproduct lyase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MFIPKTVIFEEKALEYVKGKEILKELKNKNIEIKYSKTGRVTGQRDKTPNEMYSEGKDTLVIGIRKSLDFQSCKPSAHYQ
LPLVSGCMGMCEYCYLNTQMGKRPYTKVYVNTEEILNKAEKYSRERLPEITVFEGAATSDPIPVEPYTHSLKDTIEFFGQ
KHNMKFRFVTKFTDVNSLVHLNHNRNTTIRFSINTDNIIRNFEHRTPLVIDRLKAAEKIAKAGYSLGFIIAPVFIYRGWE
KEYLDLLKNINIMFKGVPVEFEVISHRFTKRAKDNILSVFSNTTLPMVEENRKFKYGQFGYGKYIYKDEELQNIKKFFRD
SITKFFGEDNINYII

Sequences:

>Translated_335_residues
MFIPKTVIFEEKALEYVKGKEILKELKNKNIEIKYSKTGRVTGQRDKTPNEMYSEGKDTLVIGIRKSLDFQSCKPSAHYQ
LPLVSGCMGMCEYCYLNTQMGKRPYTKVYVNTEEILNKAEKYSRERLPEITVFEGAATSDPIPVEPYTHSLKDTIEFFGQ
KHNMKFRFVTKFTDVNSLVHLNHNRNTTIRFSINTDNIIRNFEHRTPLVIDRLKAAEKIAKAGYSLGFIIAPVFIYRGWE
KEYLDLLKNINIMFKGVPVEFEVISHRFTKRAKDNILSVFSNTTLPMVEENRKFKYGQFGYGKYIYKDEELQNIKKFFRD
SITKFFGEDNINYII
>Mature_335_residues
MFIPKTVIFEEKALEYVKGKEILKELKNKNIEIKYSKTGRVTGQRDKTPNEMYSEGKDTLVIGIRKSLDFQSCKPSAHYQ
LPLVSGCMGMCEYCYLNTQMGKRPYTKVYVNTEEILNKAEKYSRERLPEITVFEGAATSDPIPVEPYTHSLKDTIEFFGQ
KHNMKFRFVTKFTDVNSLVHLNHNRNTTIRFSINTDNIIRNFEHRTPLVIDRLKAAEKIAKAGYSLGFIIAPVFIYRGWE
KEYLDLLKNINIMFKGVPVEFEVISHRFTKRAKDNILSVFSNTTLPMVEENRKFKYGQFGYGKYIYKDEELQNIKKFFRD
SITKFFGEDNINYII

Specific function: Involved in repair of UV radiation-induced DNA damage during spore germination. Can repair thymine dimer 5-thyminyl-5,6- dihydrothymine (known as spore photoproduct (SP)) by in situ monomerization of SP to two thymines [H]

COG id: COG1533

COG function: function code L; DNA repair photolyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. SPL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =4.1.99.14 [H]

Molecular weight: Translated: 39194; Mature: 39194

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFIPKTVIFEEKALEYVKGKEILKELKNKNIEIKYSKTGRVTGQRDKTPNEMYSEGKDTL
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHCCCCEE
VIGIRKSLDFQSCKPSAHYQLPLVSGCMGMCEYCYLNTQMGKRPYTKVYVNTEEILNKAE
EEEEECCCCCCCCCCCCCEECHHHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHHH
KYSRERLPEITVFEGAATSDPIPVEPYTHSLKDTIEFFGQKHNMKFRFVTKFTDVNSLVH
HHHHHHCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCEEE
LNHNRNTTIRFSINTDNIIRNFEHRTPLVIDRLKAAEKIAKAGYSLGFIIAPVFIYRGWE
EECCCCEEEEEEECCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
KEYLDLLKNINIMFKGVPVEFEVISHRFTKRAKDNILSVFSNTTLPMVEENRKFKYGQFG
HHHHHHHHCCCEEEECCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEECCCCC
YGKYIYKDEELQNIKKFFRDSITKFFGEDNINYII
CCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEC
>Mature Secondary Structure
MFIPKTVIFEEKALEYVKGKEILKELKNKNIEIKYSKTGRVTGQRDKTPNEMYSEGKDTL
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHCCCCEE
VIGIRKSLDFQSCKPSAHYQLPLVSGCMGMCEYCYLNTQMGKRPYTKVYVNTEEILNKAE
EEEEECCCCCCCCCCCCCEECHHHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHHH
KYSRERLPEITVFEGAATSDPIPVEPYTHSLKDTIEFFGQKHNMKFRFVTKFTDVNSLVH
HHHHHHCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCEEE
LNHNRNTTIRFSINTDNIIRNFEHRTPLVIDRLKAAEKIAKAGYSLGFIIAPVFIYRGWE
EECCCCEEEEEEECCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
KEYLDLLKNINIMFKGVPVEFEVISHRFTKRAKDNILSVFSNTTLPMVEENRKFKYGQFG
HHHHHHHHCCCEEEECCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEECCCCC
YGKYIYKDEELQNIKKFFRDSITKFFGEDNINYII
CCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]