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The map label for this gene is sleB [H]

Identifier: 226947425

GI number: 226947425

Start: 237646

End: 238341

Strand: Direct

Name: sleB [H]

Synonym: CLM_0254

Alternate gene names: 226947425

Gene position: 237646-238341 (Clockwise)

Preceding gene: 226947424

Following gene: 226947426

Centisome position: 5.72

GC content: 35.06

Gene sequence:

>696_bases
ATGAGAAAGAATATATATTTAAAAAGAGCAATAATATATGTTTTAGCTATAATGCTAGCTTATGGCAGTACTACTCTTTA
TATATTACCTTATACAAATGCTACTAAAGCTGTAGCATATTATTATGGGCATAGAGGAGACATTATTTCACAGGTTCAAA
GAAAACTTAAAGCCTGGGGGTATTATAATGGAGGAGTAGATGGAATATTTGGCCATCAAACCTATACAGCCGTAAGATAT
TTTCAATCTAAAAATGGTTTGACGGTAGATGGAATAATTGGAGATAAAACTTTAGCTGCACTTGGAATAAAAACAGGAGC
ATCAGGATCAAGTTCAAGCTCCAATAACCAAGATGTTATGCTTTTAGCTAGACTTATAAATGGAGAGGCTAGAGGAGAAC
CTTATGAAGGTCAAGTAGCAGTAGGAGCAGTAGTTTTAAACAGAACAAGAGATCCTAAATTTCCTAATACTATAGCTGGG
GTTATATATGAACCACTTGCATTTACTGCCATAGCAGACGGACAAATAAATGCACAAATGCAGCAAACCTCTATAAATGC
AGCTAGAGATGCTTTAAATGGATGGGATCCTTCAGATGGGGCTACGTATTATTTTAATCCAGCAACTGCTACGAGTTCTT
GGATTTGGTCAAGACCGCTAATAAAAGTAATAGGTAAACATAGATTTTGTAGATAA

Upstream 100 bases:

>100_bases
TAAATTTATTTTATTTTATAAAAGAGTATTTTTTATAATTTTATGCACAAACTAATTTTAAAATAAATAGAAAAACTATT
GATTTTGTGGGAGGAGTATT

Downstream 100 bases:

>100_bases
GTAGTTGACAAATTCAAAAAATATCATATAATTATATTATAGAATTCATACTAGAATAGTCGGAAATAAAAACGTTGAAA
AAGGAAAGTAGCATAATTAA

Product: spore-cortex-lytic enzyme

Products: NA

Alternate protein names: SCLE; Germination-specific amidase [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MRKNIYLKRAIIYVLAIMLAYGSTTLYILPYTNATKAVAYYYGHRGDIISQVQRKLKAWGYYNGGVDGIFGHQTYTAVRY
FQSKNGLTVDGIIGDKTLAALGIKTGASGSSSSSNNQDVMLLARLINGEARGEPYEGQVAVGAVVLNRTRDPKFPNTIAG
VIYEPLAFTAIADGQINAQMQQTSINAARDALNGWDPSDGATYYFNPATATSSWIWSRPLIKVIGKHRFCR

Sequences:

>Translated_231_residues
MRKNIYLKRAIIYVLAIMLAYGSTTLYILPYTNATKAVAYYYGHRGDIISQVQRKLKAWGYYNGGVDGIFGHQTYTAVRY
FQSKNGLTVDGIIGDKTLAALGIKTGASGSSSSSNNQDVMLLARLINGEARGEPYEGQVAVGAVVLNRTRDPKFPNTIAG
VIYEPLAFTAIADGQINAQMQQTSINAARDALNGWDPSDGATYYFNPATATSSWIWSRPLIKVIGKHRFCR
>Mature_231_residues
MRKNIYLKRAIIYVLAIMLAYGSTTLYILPYTNATKAVAYYYGHRGDIISQVQRKLKAWGYYNGGVDGIFGHQTYTAVRY
FQSKNGLTVDGIIGDKTLAALGIKTGASGSSSSSNNQDVMLLARLINGEARGEPYEGQVAVGAVVLNRTRDPKFPNTIAG
VIYEPLAFTAIADGQINAQMQQTSINAARDALNGWDPSDGATYYFNPATATSSWIWSRPLIKVIGKHRFCR

Specific function: Probable N-acetylmuramyl-L-alanine amidase. Required for spore cortex hydrolysis during germination. May form a complex with some hydrophobic spore component, leading to a stabilization of the enzyme in a spore-bound form [H]

COG id: COG3773

COG function: function code M; Cell wall hydrolyses involved in spore germination

Gene ontology:

Cell location: Forespore. Note=Expressed in the forespore and then transported across the inner forespore membrane and deposited on the outside of the cortex [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sleB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011105
- InterPro:   IPR002477
- InterPro:   IPR014224 [H]

Pfam domain/function: PF07486 Hydrolase_2; PF01471 PG_binding_1 [H]

EC number: 3.5.1.28

Molecular weight: Translated: 25305; Mature: 25305

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKNIYLKRAIIYVLAIMLAYGSTTLYILPYTNATKAVAYYYGHRGDIISQVQRKLKAWG
CCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHCC
YYNGGVDGIFGHQTYTAVRYFQSKNGLTVDGIIGDKTLAALGIKTGASGSSSSSNNQDVM
CCCCCCCCCCCCHHHHHHHHEECCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCHHH
LLARLINGEARGEPYEGQVAVGAVVLNRTRDPKFPNTIAGVIYEPLAFTAIADGQINAQM
HHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHEEEEECCCCCCHH
QQTSINAARDALNGWDPSDGATYYFNPATATSSWIWSRPLIKVIGKHRFCR
HHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCHHHHCHHHHHHHHCCCCC
>Mature Secondary Structure
MRKNIYLKRAIIYVLAIMLAYGSTTLYILPYTNATKAVAYYYGHRGDIISQVQRKLKAWG
CCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHCC
YYNGGVDGIFGHQTYTAVRYFQSKNGLTVDGIIGDKTLAALGIKTGASGSSSSSNNQDVM
CCCCCCCCCCCCHHHHHHHHEECCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCHHH
LLARLINGEARGEPYEGQVAVGAVVLNRTRDPKFPNTIAGVIYEPLAFTAIADGQINAQM
HHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHEEEEECCCCCCHH
QQTSINAARDALNGWDPSDGATYYFNPATATSSWIWSRPLIKVIGKHRFCR
HHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCHHHHCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8752358; 8081503; 10197998 [H]