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The map label for this gene is cysK [H]

Identifier: 226947426

GI number: 226947426

Start: 238705

End: 239619

Strand: Direct

Name: cysK [H]

Synonym: CLM_0255

Alternate gene names: 226947426

Gene position: 238705-239619 (Clockwise)

Preceding gene: 226947425

Following gene: 226947427

Centisome position: 5.74

GC content: 32.9

Gene sequence:

>915_bases
ATGTTGTATGAAAGTTCCATAGATCTAATAGGAAATACACCTATGTTTAAATTAAATAATATGCAGGAAGAAAATATGGC
AGATGTTTATGTAAAATTAGAAAAATTTAATCCTGGTGGTAGTATAAAAGATAGAGCAGCTTTAGGAATGATTGAAGAGG
CAGAAAAAATGGGGAAAATAAAACCAGGAGATATAATAGTAGAACCTACTAGTGGAAACACAGGTATAGGACTTGCTATG
GTTGGAAGATTAAAAGGTTATAAAGTAATTATTGTTATGCCGGATTCTATGAGTATAGAGAGAAGAAACATGATAAAAGC
TTACGGAGCAGAATTGGTTCTTACAGAAGGAAATAAAGGAATGACAGGTGCTATAGAAAAGGCAGAAGAATTAGCTAGAG
ATAAAAAGGGCTACTTTATTCCTCAACAATTTTCAAATAGAGCTAATTCAAAAAAACATTATGAAACTACCGCTGTTGAG
ATATTAAAAGATGTAGAGGATTTAGATGCTTTTGTAGCTTCAGTAGGTACAGGAGGTACTATAGCGGGTATTGGAAGAAG
ATTAAAAGAATTTAATAAAAATATAAAAGTTGTAGCTGTGGAACCTTATAACTCTCCAGTAATATCAGGAGGAAAAGCCG
CACCACACAAAATACAAGGTATAGGCGCAGGATTTATACCAGATGTATATGAGAAAGATGTAGTGGATGATGTAATGACT
ATAACAGATGAGGAATCTTATGAATATGCAAGAAGATTTGGTGCAGAAGAAGGAATATTAGTAGGTATATCTTCTGGCGC
AAATATAGCAGCAGCTATAAAAATAGCTAAAAAATTAGGAAAAGGTAAAAAGGTTGTAACTGTAGCTCCAGATGGTGGAG
AAAAATATATATCCACAGGGTTATATGATAAATAA

Upstream 100 bases:

>100_bases
CTAACTAGAGTGGAACCACGGAGAATATACTTCGTCTCTATATTTTTATAGAGATGAAGTTTTTTTATATTAAGTTTATT
TTAAAAAAGGGGAGATTGAT

Downstream 100 bases:

>100_bases
AAGAGGTGAATTATATGAAAAATCCTTTTAAAGTTTTAATATACGATTTAAAAAATGCTAAGGAGAAAGATCCAGCAGCA
AGAAACATTTTAGAAGTTTT

Product: cysteine synthase A

Products: NA

Alternate protein names: CSase; O-acetylserine (thiol)-lyase; OAS-TL; O-acetylserine sulfhydrylase [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MLYESSIDLIGNTPMFKLNNMQEENMADVYVKLEKFNPGGSIKDRAALGMIEEAEKMGKIKPGDIIVEPTSGNTGIGLAM
VGRLKGYKVIIVMPDSMSIERRNMIKAYGAELVLTEGNKGMTGAIEKAEELARDKKGYFIPQQFSNRANSKKHYETTAVE
ILKDVEDLDAFVASVGTGGTIAGIGRRLKEFNKNIKVVAVEPYNSPVISGGKAAPHKIQGIGAGFIPDVYEKDVVDDVMT
ITDEESYEYARRFGAEEGILVGISSGANIAAAIKIAKKLGKGKKVVTVAPDGGEKYISTGLYDK

Sequences:

>Translated_304_residues
MLYESSIDLIGNTPMFKLNNMQEENMADVYVKLEKFNPGGSIKDRAALGMIEEAEKMGKIKPGDIIVEPTSGNTGIGLAM
VGRLKGYKVIIVMPDSMSIERRNMIKAYGAELVLTEGNKGMTGAIEKAEELARDKKGYFIPQQFSNRANSKKHYETTAVE
ILKDVEDLDAFVASVGTGGTIAGIGRRLKEFNKNIKVVAVEPYNSPVISGGKAAPHKIQGIGAGFIPDVYEKDVVDDVMT
ITDEESYEYARRFGAEEGILVGISSGANIAAAIKIAKKLGKGKKVVTVAPDGGEKYISTGLYDK
>Mature_304_residues
MLYESSIDLIGNTPMFKLNNMQEENMADVYVKLEKFNPGGSIKDRAALGMIEEAEKMGKIKPGDIIVEPTSGNTGIGLAM
VGRLKGYKVIIVMPDSMSIERRNMIKAYGAELVLTEGNKGMTGAIEKAEELARDKKGYFIPQQFSNRANSKKHYETTAVE
ILKDVEDLDAFVASVGTGGTIAGIGRRLKEFNKNIKVVAVEPYNSPVISGGKAAPHKIQGIGAGFIPDVYEKDVVDDVMT
ITDEESYEYARRFGAEEGILVGISSGANIAAAIKIAKKLGKGKKVVTVAPDGGEKYISTGLYDK

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=306, Percent_Identity=41.1764705882353, Blast_Score=234, Evalue=6e-62,
Organism=Homo sapiens, GI295821200, Length=306, Percent_Identity=41.1764705882353, Blast_Score=234, Evalue=6e-62,
Organism=Homo sapiens, GI4557415, Length=306, Percent_Identity=41.1764705882353, Blast_Score=234, Evalue=6e-62,
Organism=Homo sapiens, GI11345492, Length=281, Percent_Identity=26.3345195729537, Blast_Score=82, Evalue=6e-16,
Organism=Escherichia coli, GI1788754, Length=312, Percent_Identity=48.3974358974359, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI2367138, Length=294, Percent_Identity=42.5170068027211, Blast_Score=236, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI17535051, Length=305, Percent_Identity=46.5573770491803, Blast_Score=278, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17562970, Length=305, Percent_Identity=45.5737704918033, Blast_Score=263, Evalue=7e-71,
Organism=Caenorhabditis elegans, GI32566674, Length=300, Percent_Identity=45.3333333333333, Blast_Score=258, Evalue=3e-69,
Organism=Caenorhabditis elegans, GI115535073, Length=302, Percent_Identity=49.0066225165563, Blast_Score=253, Evalue=8e-68,
Organism=Caenorhabditis elegans, GI17561720, Length=306, Percent_Identity=44.7712418300654, Blast_Score=234, Evalue=6e-62,
Organism=Caenorhabditis elegans, GI32566672, Length=212, Percent_Identity=43.8679245283019, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17534315, Length=320, Percent_Identity=38.75, Blast_Score=174, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI25147552, Length=319, Percent_Identity=37.3040752351097, Blast_Score=164, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI17561716, Length=119, Percent_Identity=42.8571428571429, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17539800, Length=342, Percent_Identity=27.1929824561404, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI71996324, Length=330, Percent_Identity=26.0606060606061, Blast_Score=74, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6321594, Length=312, Percent_Identity=38.7820512820513, Blast_Score=209, Evalue=5e-55,
Organism=Saccharomyces cerevisiae, GI6321449, Length=322, Percent_Identity=33.2298136645963, Blast_Score=147, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24643623, Length=308, Percent_Identity=40.2597402597403, Blast_Score=213, Evalue=1e-55,
Organism=Drosophila melanogaster, GI20129101, Length=308, Percent_Identity=40.2597402597403, Blast_Score=213, Evalue=1e-55,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 32823; Mature: 32823

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00165 DEHYDRATASE_SER_THR ; PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYESSIDLIGNTPMFKLNNMQEENMADVYVKLEKFNPGGSIKDRAALGMIEEAEKMGKI
CCCCCCCCCCCCCCEEEECCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCC
KPGDIIVEPTSGNTGIGLAMVGRLKGYKVIIVMPDSMSIERRNMIKAYGAELVLTEGNKG
CCCCEEEECCCCCCCCHHEEEECCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCC
MTGAIEKAEELARDKKGYFIPQQFSNRANSKKHYETTAVEILKDVEDLDAFVASVGTGGT
CCHHHHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
IAGIGRRLKEFNKNIKVVAVEPYNSPVISGGKAAPHKIQGIGAGFIPDVYEKDVVDDVMT
HHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHH
ITDEESYEYARRFGAEEGILVGISSGANIAAAIKIAKKLGKGKKVVTVAPDGGEKYISTG
HCCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHCCC
LYDK
CCCC
>Mature Secondary Structure
MLYESSIDLIGNTPMFKLNNMQEENMADVYVKLEKFNPGGSIKDRAALGMIEEAEKMGKI
CCCCCCCCCCCCCCEEEECCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCC
KPGDIIVEPTSGNTGIGLAMVGRLKGYKVIIVMPDSMSIERRNMIKAYGAELVLTEGNKG
CCCCEEEECCCCCCCCHHEEEECCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCC
MTGAIEKAEELARDKKGYFIPQQFSNRANSKKHYETTAVEILKDVEDLDAFVASVGTGGT
CCHHHHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
IAGIGRRLKEFNKNIKVVAVEPYNSPVISGGKAAPHKIQGIGAGFIPDVYEKDVVDDVMT
HHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHH
ITDEESYEYARRFGAEEGILVGISSGANIAAAIKIAKKLGKGKKVVTVAPDGGEKYISTG
HCCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHCCC
LYDK
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA