Definition Clostridium botulinum A2 str. Kyoto chromosome, complete genome.
Accession NC_012563
Length 4,155,278

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The map label for this gene is 226947424

Identifier: 226947424

GI number: 226947424

Start: 236152

End: 237417

Strand: Direct

Name: 226947424

Synonym: CLM_0253

Alternate gene names: NA

Gene position: 236152-237417 (Clockwise)

Preceding gene: 226947422

Following gene: 226947425

Centisome position: 5.68

GC content: 27.41

Gene sequence:

>1266_bases
ATGAAGAAGCTACAAAGTTTTTTCTTAAGTAAAATTATTAATAAAAAAGTTTATGATGAGTATGGTGATTATATAGGTAA
ACTAATTGATATTTATGTGACTTCTGAGGATGATTATCCAAGAGCTATAGCCTATAAGATAAAAAAGGGAAGAGAAGTAG
TAAATTATGAGTTTAAAAACATTTCTTTTTATGATGATGAAGGCAAAGCCATTATAAAAGTAATAGGAACTAGAGATATA
ATATTAAGAAAATATTCCTATTTATTGTCACAACATCTTTTAAACAAACAAATAATTGATATAAATGGTAAAAAGATGGT
TAAAGTGCACGACTTAAGAATTGGAGAAATCGCAGGAGAGTATAAAGTAATAGCTGTAGATACAGGTGCGCTAGCATTAA
GTAGAAGAGTAGGAATGGAAAATTTATTAAGGAGTATTTATAAAATATTTAATAAAAAAATAGAGGATACTTTAATAATG
TGGGATAGTGTGGAATCCTTAGAAATGGTAAATAATAATTTGAAATTATCTGTGTCTTATCAAAAACTCTCAACATTACA
TCCAGCAGATTTAGCAGATATTATAGAAGATATGAATGATAGTTATAGAAAATTAGTTTTTGAAAGTTTAGATGATGATT
TAGCTGCGGATACTTTTGAAGAGATAGACTTAGAAGTAAAAACAGAAATGTTAGAAGAAATGACATCCTCCAAAAAAGCC
GAAATCATATATAATATGTCAAAAGATGAAGCTGCAGATATATTGGCACAAATGAAAGAGGAAGAAGTAGAGCAAATTCT
TTCTATTATGGAGGAAAGAGATGCTAAGGATATAAGAAAACTTATGGATTATAAAGAAGAAACTGCAGGAAGCATAATGA
ATAAAGATTTTATATCGTTTAATGTGAACATAACTGCAGAAGAAACTATAGATTTATTAAGAGAAATAAAACCTAAAGAT
GAAGAGGCTTATTATATATATATCGTAGATGAAAAAGAACAATTAAAAGGGGCTATATCTCTTACAGATTTAATAATATG
TACTCCAGGAGCTAAATTAAAAGATATAATGGATAAAGATATAGTAAAAGTTAAAGATACAGACCATATAAATGAAGCTG
TAGAATTGGCAATAAAATATGATTTATTATCTATTCCTGTAATTGAGGGAGAAAATAAATTATGTGGAACTATATCTATA
GATGATATTATAGAGGATGTATTTGCACCTATGTGGAGAAAGAAAGTTAAAATAGTTGCGGTTTAA

Upstream 100 bases:

>100_bases
AGAAAAATTATATTATTTTGTAGGGGAATATTTATTTAAGCGAGAAATACTTATTTGTAGCTTAGGATATTCCTTTACAG
CTGTGAAAGGAGGAATATTT

Downstream 100 bases:

>100_bases
ATTATATTTATTAAAAAACATGGTGCCCTAAAATAGATTTAATTTTAATATAATAAATATAAAAAACACATGTAATAAAC
TTATCCATAAGTTTATTACA

Product: PRC-barrel domain/MgtE domain/CBS domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MKKLQSFFLSKIINKKVYDEYGDYIGKLIDIYVTSEDDYPRAIAYKIKKGREVVNYEFKNISFYDDEGKAIIKVIGTRDI
ILRKYSYLLSQHLLNKQIIDINGKKMVKVHDLRIGEIAGEYKVIAVDTGALALSRRVGMENLLRSIYKIFNKKIEDTLIM
WDSVESLEMVNNNLKLSVSYQKLSTLHPADLADIIEDMNDSYRKLVFESLDDDLAADTFEEIDLEVKTEMLEEMTSSKKA
EIIYNMSKDEAADILAQMKEEEVEQILSIMEERDAKDIRKLMDYKEETAGSIMNKDFISFNVNITAEETIDLLREIKPKD
EEAYYIYIVDEKEQLKGAISLTDLIICTPGAKLKDIMDKDIVKVKDTDHINEAVELAIKYDLLSIPVIEGENKLCGTISI
DDIIEDVFAPMWRKKVKIVAV

Sequences:

>Translated_421_residues
MKKLQSFFLSKIINKKVYDEYGDYIGKLIDIYVTSEDDYPRAIAYKIKKGREVVNYEFKNISFYDDEGKAIIKVIGTRDI
ILRKYSYLLSQHLLNKQIIDINGKKMVKVHDLRIGEIAGEYKVIAVDTGALALSRRVGMENLLRSIYKIFNKKIEDTLIM
WDSVESLEMVNNNLKLSVSYQKLSTLHPADLADIIEDMNDSYRKLVFESLDDDLAADTFEEIDLEVKTEMLEEMTSSKKA
EIIYNMSKDEAADILAQMKEEEVEQILSIMEERDAKDIRKLMDYKEETAGSIMNKDFISFNVNITAEETIDLLREIKPKD
EEAYYIYIVDEKEQLKGAISLTDLIICTPGAKLKDIMDKDIVKVKDTDHINEAVELAIKYDLLSIPVIEGENKLCGTISI
DDIIEDVFAPMWRKKVKIVAV
>Mature_421_residues
MKKLQSFFLSKIINKKVYDEYGDYIGKLIDIYVTSEDDYPRAIAYKIKKGREVVNYEFKNISFYDDEGKAIIKVIGTRDI
ILRKYSYLLSQHLLNKQIIDINGKKMVKVHDLRIGEIAGEYKVIAVDTGALALSRRVGMENLLRSIYKIFNKKIEDTLIM
WDSVESLEMVNNNLKLSVSYQKLSTLHPADLADIIEDMNDSYRKLVFESLDDDLAADTFEEIDLEVKTEMLEEMTSSKKA
EIIYNMSKDEAADILAQMKEEEVEQILSIMEERDAKDIRKLMDYKEETAGSIMNKDFISFNVNITAEETIDLLREIKPKD
EEAYYIYIVDEKEQLKGAISLTDLIICTPGAKLKDIMDKDIVKVKDTDHINEAVELAIKYDLLSIPVIEGENKLCGTISI
DDIIEDVFAPMWRKKVKIVAV

Specific function: Acts as a magnesium transporter [H]

COG id: COG2239

COG function: function code P; Mg/Co/Ni transporter MgtE (contains CBS domain)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 2 CBS domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000644
- InterPro:   IPR006668
- InterPro:   IPR006667
- InterPro:   IPR006669 [H]

Pfam domain/function: PF00571 CBS; PF01769 MgtE; PF03448 MgtE_N [H]

EC number: NA

Molecular weight: Translated: 48450; Mature: 48450

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLQSFFLSKIINKKVYDEYGDYIGKLIDIYVTSEDDYPRAIAYKIKKGREVVNYEFKN
CCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHCCCEEEEEEECC
ISFYDDEGKAIIKVIGTRDIILRKYSYLLSQHLLNKQIIDINGKKMVKVHDLRIGEIAGE
EEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEEECEEEECCCCC
YKVIAVDTGALALSRRVGMENLLRSIYKIFNKKIEDTLIMWDSVESLEMVNNNLKLSVSY
EEEEEEECCHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHCCCEEEEEEH
QKLSTLHPADLADIIEDMNDSYRKLVFESLDDDLAADTFEEIDLEVKTEMLEEMTSSKKA
HHHCCCCCCHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCE
EIIYNMSKDEAADILAQMKEEEVEQILSIMEERDAKDIRKLMDYKEETAGSIMNKDFISF
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEE
NVNITAEETIDLLREIKPKDEEAYYIYIVDEKEQLKGAISLTDLIICTPGAKLKDIMDKD
EEEEEHHHHHHHHHHCCCCCCCEEEEEEECCHHHHCCCEEEEEEEEECCCCHHHHHHHCC
IVKVKDTDHINEAVELAIKYDLLSIPVIEGENKLCGTISIDDIIEDVFAPMWRKKVKIVA
CEEECCCHHHHHHHHHHHEEEEEEEEEECCCCCEEEECCHHHHHHHHHHHHHHCCEEEEE
V
C
>Mature Secondary Structure
MKKLQSFFLSKIINKKVYDEYGDYIGKLIDIYVTSEDDYPRAIAYKIKKGREVVNYEFKN
CCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHCCCEEEEEEECC
ISFYDDEGKAIIKVIGTRDIILRKYSYLLSQHLLNKQIIDINGKKMVKVHDLRIGEIAGE
EEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEEECEEEECCCCC
YKVIAVDTGALALSRRVGMENLLRSIYKIFNKKIEDTLIMWDSVESLEMVNNNLKLSVSY
EEEEEEECCHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHCCCEEEEEEH
QKLSTLHPADLADIIEDMNDSYRKLVFESLDDDLAADTFEEIDLEVKTEMLEEMTSSKKA
HHHCCCCCCHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCE
EIIYNMSKDEAADILAQMKEEEVEQILSIMEERDAKDIRKLMDYKEETAGSIMNKDFISF
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEE
NVNITAEETIDLLREIKPKDEEAYYIYIVDEKEQLKGAISLTDLIICTPGAKLKDIMDKD
EEEEEHHHHHHHHHHCCCCCCCEEEEEEECCHHHHCCCEEEEEEEEECCCCHHHHHHHCC
IVKVKDTDHINEAVELAIKYDLLSIPVIEGENKLCGTISIDDIIEDVFAPMWRKKVKIVA
CEEECCCHHHHHHHHHHHEEEEEEEEEECCCCCEEEECCHHHHHHHHHHHHHHCCEEEEE
V
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA