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The map label for this gene is actII-2 [H]

Identifier: 226225394

GI number: 226225394

Start: 2901465

End: 2902877

Strand: Direct

Name: actII-2 [H]

Synonym: Lm4b_02817

Alternate gene names: 226225394

Gene position: 2901465-2902877 (Clockwise)

Preceding gene: 226225391

Following gene: 226225400

Centisome position: 99.61

GC content: 40.2

Gene sequence:

>1413_bases
ATGGAACAGACGAATAAACGTAATACTTTAGCACTTATTTCAATTATGCTTGGAGCTTTTATTTCATTATTAGATACAAC
AATTGTCAACGTTGCCTTACCTGATATCACAACTGCTCTACATGCCACGAGTGAAACCATCGAATGGGTAATTTCAGGCT
ATGCGCTTGCTTTTGGACTTGTACTTATTTTAGCTGGTCGACTCGGGGATAAATTTGGCCGAAAAAATATCTACATTATC
GGGATTACGCTATTTCTAATTATGAGTGTGACAGCTGGTTTTGCAGATTCCGAAAATAGTCTAATTATTTCCCGGGTAAT
ACAGGGACTTGCTGCCGGATTATTTTTCCCGCAAATCAATGCCACAATTATGGATATGTATTCTGGAAAAAGCCTTGGGA
AAATCTTCGGAATTCTTGGATCTGTTATTGGTGTGGGAACTGCCATCGGACCTCTTACAGGAGGTCTTTTAATTGAACTT
TTCGGAACTACCAATGGTTGGCGTGCAGTTTTTTTCGTTAACGTACCTTTTGTGTTAGTAACGCTAGTCCTAGCAATGCT
TTATCTTCCCAAAAGAACTGTCTCCACGAAAAAAATTAGTTTTGATTTGCCTGGAGTTGGGCTACTAACCATTGCACTTT
TACTATTACTTTTTCCACTTATCTCAAACGGCGCAAATGACTTCAAACCAGTGGATTACTTTTTAATGGCGCTATCTATT
CCACTCTTTATCATTCTTTACAAATGGAGCGTCTACCAAGAAAAGAAAGGAAATCAGCCATTAATTGCTCCCAACCTACT
TAAAAATAATCAATTTGTTTCAGGTATGCTTTTATCGCTTGTATACTTTGCCGCTTTTACTAGTATTTTCTTCGTCCTAT
CGCTAACTTGGCAAACTGGTTTCAATCGTTCGGCTATTTTATCCGGGCTTGCAATTAGTCCTTTTGCGTTAGGTAGTGTG
TTAGCTGCCTCTAATAGTTACCGACTAATCCCTATATTAGGTAGAAAGCTCTTAATGCTTGGAGTTGCGCTTGTTATCAT
CGGGCTTGGCACCGTGTCTATTGTTTTCCATTTGAACGACGGCGCTTTTTCTGCTTGGCTCTTATTCTTACCTCTTTTCA
TCGCTGGTATCGGCAGTGGTTTGACTATTGCACCATTGAATAGTTTCACCCTTTCCACAGTAACTGGTGTGGATCGCGGC
GGAGCTAGTGGTATGTTTAATACAGCGCAGCGAATTGGATCGTCCTTTGGGATTGCTGTTGTTGGCTCAGTCTTCTTCCG
CACACTTGGAAATACTGCCGCTACTTCAAAAGCAAGCGCCTTTTCCGACAGTTTGCAAATGAGTATGTACGTCAATATTG
TTCTGCTCGTTGTTTGTTTCCTACTTGTATTCCGGTTACCGAAAAATATTTAA

Upstream 100 bases:

>100_bases
AAAATGTTTCACGTGAAACATTCGCTCCTTCTTTATCATCAGAGTTTTTAGGTTTATAATGGAAAATATCTCAAAAAGCT
TCAAAAAAGGAGAATGATTC

Downstream 100 bases:

>100_bases
AAAAGAGACATGACTTTGCGTTAAAGTCATGTCTCTTTTCTTATGCTAAATAGAAAACTTCTTTTAAATCTAATGCAACC
GGGCTGTTATTAGGATTTGA

Product: transmembrane efflux protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MEQTNKRNTLALISIMLGAFISLLDTTIVNVALPDITTALHATSETIEWVISGYALAFGLVLILAGRLGDKFGRKNIYII
GITLFLIMSVTAGFADSENSLIISRVIQGLAAGLFFPQINATIMDMYSGKSLGKIFGILGSVIGVGTAIGPLTGGLLIEL
FGTTNGWRAVFFVNVPFVLVTLVLAMLYLPKRTVSTKKISFDLPGVGLLTIALLLLLFPLISNGANDFKPVDYFLMALSI
PLFIILYKWSVYQEKKGNQPLIAPNLLKNNQFVSGMLLSLVYFAAFTSIFFVLSLTWQTGFNRSAILSGLAISPFALGSV
LAASNSYRLIPILGRKLLMLGVALVIIGLGTVSIVFHLNDGAFSAWLLFLPLFIAGIGSGLTIAPLNSFTLSTVTGVDRG
GASGMFNTAQRIGSSFGIAVVGSVFFRTLGNTAATSKASAFSDSLQMSMYVNIVLLVVCFLLVFRLPKNI

Sequences:

>Translated_470_residues
MEQTNKRNTLALISIMLGAFISLLDTTIVNVALPDITTALHATSETIEWVISGYALAFGLVLILAGRLGDKFGRKNIYII
GITLFLIMSVTAGFADSENSLIISRVIQGLAAGLFFPQINATIMDMYSGKSLGKIFGILGSVIGVGTAIGPLTGGLLIEL
FGTTNGWRAVFFVNVPFVLVTLVLAMLYLPKRTVSTKKISFDLPGVGLLTIALLLLLFPLISNGANDFKPVDYFLMALSI
PLFIILYKWSVYQEKKGNQPLIAPNLLKNNQFVSGMLLSLVYFAAFTSIFFVLSLTWQTGFNRSAILSGLAISPFALGSV
LAASNSYRLIPILGRKLLMLGVALVIIGLGTVSIVFHLNDGAFSAWLLFLPLFIAGIGSGLTIAPLNSFTLSTVTGVDRG
GASGMFNTAQRIGSSFGIAVVGSVFFRTLGNTAATSKASAFSDSLQMSMYVNIVLLVVCFLLVFRLPKNI
>Mature_470_residues
MEQTNKRNTLALISIMLGAFISLLDTTIVNVALPDITTALHATSETIEWVISGYALAFGLVLILAGRLGDKFGRKNIYII
GITLFLIMSVTAGFADSENSLIISRVIQGLAAGLFFPQINATIMDMYSGKSLGKIFGILGSVIGVGTAIGPLTGGLLIEL
FGTTNGWRAVFFVNVPFVLVTLVLAMLYLPKRTVSTKKISFDLPGVGLLTIALLLLLFPLISNGANDFKPVDYFLMALSI
PLFIILYKWSVYQEKKGNQPLIAPNLLKNNQFVSGMLLSLVYFAAFTSIFFVLSLTWQTGFNRSAILSGLAISPFALGSV
LAASNSYRLIPILGRKLLMLGVALVIIGLGTVSIVFHLNDGAFSAWLLFLPLFIAGIGSGLTIAPLNSFTLSTVTGVDRG
GASGMFNTAQRIGSSFGIAVVGSVFFRTLGNTAATSKASAFSDSLQMSMYVNIVLLVVCFLLVFRLPKNI

Specific function: Promotes the efflux of actinorhodin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=448, Percent_Identity=27.4553571428571, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI87081983, Length=414, Percent_Identity=29.7101449275362, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1788710, Length=446, Percent_Identity=23.542600896861, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1789042, Length=457, Percent_Identity=25.601750547046, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1788392, Length=458, Percent_Identity=23.5807860262009, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790146, Length=174, Percent_Identity=25.8620689655172, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI48994889, Length=171, Percent_Identity=27.4853801169591, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323735, Length=175, Percent_Identity=29.7142857142857, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323520, Length=301, Percent_Identity=22.9235880398671, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322958, Length=410, Percent_Identity=22.6829268292683, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50276; Mature: 50276

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQTNKRNTLALISIMLGAFISLLDTTIVNVALPDITTALHATSETIEWVISGYALAFGL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VLILAGRLGDKFGRKNIYIIGITLFLIMSVTAGFADSENSLIISRVIQGLAAGLFFPQIN
HHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
ATIMDMYSGKSLGKIFGILGSVIGVGTAIGPLTGGLLIELFGTTNGWRAVFFVNVPFVLV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCEEEEEECCHHHHH
TLVLAMLYLPKRTVSTKKISFDLPGVGLLTIALLLLLFPLISNGANDFKPVDYFLMALSI
HHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
PLFIILYKWSVYQEKKGNQPLIAPNLLKNNQFVSGMLLSLVYFAAFTSIFFVLSLTWQTG
HHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
FNRSAILSGLAISPFALGSVLAASNSYRLIPILGRKLLMLGVALVIIGLGTVSIVFHLND
CCHHHHHCCCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCCEEEEEEECC
GAFSAWLLFLPLFIAGIGSGLTIAPLNSFTLSTVTGVDRGGASGMFNTAQRIGSSFGIAV
CHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHCCCCCCCCCCHHHHHHHCCCCCCHHH
VGSVFFRTLGNTAATSKASAFSDSLQMSMYVNIVLLVVCFLLVFRLPKNI
HHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEQTNKRNTLALISIMLGAFISLLDTTIVNVALPDITTALHATSETIEWVISGYALAFGL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VLILAGRLGDKFGRKNIYIIGITLFLIMSVTAGFADSENSLIISRVIQGLAAGLFFPQIN
HHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
ATIMDMYSGKSLGKIFGILGSVIGVGTAIGPLTGGLLIELFGTTNGWRAVFFVNVPFVLV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCEEEEEECCHHHHH
TLVLAMLYLPKRTVSTKKISFDLPGVGLLTIALLLLLFPLISNGANDFKPVDYFLMALSI
HHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
PLFIILYKWSVYQEKKGNQPLIAPNLLKNNQFVSGMLLSLVYFAAFTSIFFVLSLTWQTG
HHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
FNRSAILSGLAISPFALGSVLAASNSYRLIPILGRKLLMLGVALVIIGLGTVSIVFHLND
CCHHHHHCCCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCCEEEEEEECC
GAFSAWLLFLPLFIAGIGSGLTIAPLNSFTLSTVTGVDRGGASGMFNTAQRIGSSFGIAV
CHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHCCCCCCCCCCHHHHHHHCCCCCCHHH
VGSVFFRTLGNTAATSKASAFSDSLQMSMYVNIVLLVVCFLLVFRLPKNI
HHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1878971; 12000953 [H]