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The map label for this gene is ycjW [H]

Identifier: 226225391

GI number: 226225391

Start: 2897478

End: 2898503

Strand: Direct

Name: ycjW [H]

Synonym: Lm4b_02814

Alternate gene names: 226225391

Gene position: 2897478-2898503 (Clockwise)

Preceding gene: 226225379

Following gene: 226225394

Centisome position: 99.48

GC content: 37.82

Gene sequence:

>1026_bases
TTGGCATCAACCATATATGATATAGCAAAACATGCGGGAGTTTCAAAATCAACGGTATCTAGAGTACTAAACAATCAAGC
TAACATTTCCGAAGAATCACGAAAAAAAGTACTAGAAGCGATTGACGAACTAAACTACCAGCCAAGCAAACTAGCGCGCG
CACTTACCTCTTCGGGCTTTGATGCTATCATGGTTATTTCAAATCGCTCTACTACAACCACAACCGGAAACCCATTTTTT
TCCGAAATAATTCAATCGATTTCCACTCAAGCTGAGTTGGAAAATTTCGACCTAATTCTTCAAACTGCTAAAAACAGCGA
AGACGAACTTAAAAAATGCCTCTCCAAAATTCAAGAAAAAATGATAAAAGGCATTATTATGCTAAGTTCTCCCGCTGATG
AAGATTTTTTTCACCAATTAGACCCATATAATATCCCCATCGTCGTTACCGGAAAAGTAGAAGGTCATTACAAAAATATA
TACTCTGTTGACACCGACAATTTTGGCGACAGTTATGCTCTAACAAAACATTTAATCAAGCAAGGTCATCAAAAAATCGC
TTGTATCCATGCTCCACTCGATTATCACGTGTCGATAGATCGTCTTGCTGGTTTCCGCAGCTGCCTGTTTGACCACCAAT
TAGATTTACGTAACGACTGGATTATTGATAGCGGTTATAGTATAGAAGATAGCTACAAAGCAGCACTACGCCTAATGGAA
GGCCCAGATAAACCAACCGCAGTTTTCGCAACCGACGATTTAAAGGTCCTTAGCATTTACAAAATGGCCGCCGATAAAAA
CTTACAAATCCCTGCAGATTTTTCCGTCATCGGCTATAATGATAAAGTAGCGTCATCTTTCCTATCACCACCACTTACTT
CTATCGACATTCCAATCAATAAACTAGGAAAAAAAGCGACTAACTTATTATTCCGCCTAATTCATCAAGATAAAAATGTA
CCAAAAACAACTATTATTAAAACCGAAATGATTGAACGTGAATCCATTCAGAAAATAAACGTCTAA

Upstream 100 bases:

>100_bases
AACATCTAGTTGGACATTTACTAGAAAATTGAGTAATATAAACATGGATGATAATGACCGGAACCGATACCGGAAACAAT
AAAGAGAAAGGAAGATTACC

Downstream 100 bases:

>100_bases
ACTTATTTTCTGAGTTTACTGGTCCTGTAGCTCAGTTGGGAGAGTATCACCTTGACATGGTGGGGGTCGCTGGTTCGAGA
CCAGTCGGGACCATTTATAA

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MASTIYDIAKHAGVSKSTVSRVLNNQANISEESRKKVLEAIDELNYQPSKLARALTSSGFDAIMVISNRSTTTTTGNPFF
SEIIQSISTQAELENFDLILQTAKNSEDELKKCLSKIQEKMIKGIIMLSSPADEDFFHQLDPYNIPIVVTGKVEGHYKNI
YSVDTDNFGDSYALTKHLIKQGHQKIACIHAPLDYHVSIDRLAGFRSCLFDHQLDLRNDWIIDSGYSIEDSYKAALRLME
GPDKPTAVFATDDLKVLSIYKMAADKNLQIPADFSVIGYNDKVASSFLSPPLTSIDIPINKLGKKATNLLFRLIHQDKNV
PKTTIIKTEMIERESIQKINV

Sequences:

>Translated_341_residues
MASTIYDIAKHAGVSKSTVSRVLNNQANISEESRKKVLEAIDELNYQPSKLARALTSSGFDAIMVISNRSTTTTTGNPFF
SEIIQSISTQAELENFDLILQTAKNSEDELKKCLSKIQEKMIKGIIMLSSPADEDFFHQLDPYNIPIVVTGKVEGHYKNI
YSVDTDNFGDSYALTKHLIKQGHQKIACIHAPLDYHVSIDRLAGFRSCLFDHQLDLRNDWIIDSGYSIEDSYKAALRLME
GPDKPTAVFATDDLKVLSIYKMAADKNLQIPADFSVIGYNDKVASSFLSPPLTSIDIPINKLGKKATNLLFRLIHQDKNV
PKTTIIKTEMIERESIQKINV
>Mature_340_residues
ASTIYDIAKHAGVSKSTVSRVLNNQANISEESRKKVLEAIDELNYQPSKLARALTSSGFDAIMVISNRSTTTTTGNPFFS
EIIQSISTQAELENFDLILQTAKNSEDELKKCLSKIQEKMIKGIIMLSSPADEDFFHQLDPYNIPIVVTGKVEGHYKNIY
SVDTDNFGDSYALTKHLIKQGHQKIACIHAPLDYHVSIDRLAGFRSCLFDHQLDLRNDWIIDSGYSIEDSYKAALRLMEG
PDKPTAVFATDDLKVLSIYKMAADKNLQIPADFSVIGYNDKVASSFLSPPLTSIDIPINKLGKKATNLLFRLIHQDKNVP
KTTIIKTEMIERESIQKINV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787580, Length=330, Percent_Identity=60.3030303030303, Blast_Score=418, Evalue=1e-118,
Organism=Escherichia coli, GI1787948, Length=335, Percent_Identity=30.7462686567164, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1790194, Length=320, Percent_Identity=31.25, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1790369, Length=340, Percent_Identity=28.2352941176471, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1789068, Length=341, Percent_Identity=28.1524926686217, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1788474, Length=311, Percent_Identity=27.9742765273312, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1786540, Length=341, Percent_Identity=27.2727272727273, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1789202, Length=300, Percent_Identity=27.3333333333333, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1787906, Length=338, Percent_Identity=21.301775147929, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI48994940, Length=324, Percent_Identity=24.3827160493827, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786268, Length=337, Percent_Identity=22.5519287833828, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1790689, Length=334, Percent_Identity=23.0538922155689, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38134; Mature: 38003

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASTIYDIAKHAGVSKSTVSRVLNNQANISEESRKKVLEAIDELNYQPSKLARALTSSGF
CCCHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCC
DAIMVISNRSTTTTTGNPFFSEIIQSISTQAELENFDLILQTAKNSEDELKKCLSKIQEK
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHH
MIKGIIMLSSPADEDFFHQLDPYNIPIVVTGKVEGHYKNIYSVDTDNFGDSYALTKHLIK
HHHCEEEECCCCCHHHHHHCCCCCCCEEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHH
QGHQKIACIHAPLDYHVSIDRLAGFRSCLFDHQLDLRNDWIIDSGYSIEDSYKAALRLME
CCCCEEEEEECCCCCEEEHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHC
GPDKPTAVFATDDLKVLSIYKMAADKNLQIPADFSVIGYNDKVASSFLSPPLTSIDIPIN
CCCCCEEEEEECCCEEHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHCCCCCCEECCCHH
KLGKKATNLLFRLIHQDKNVPKTTIIKTEMIERESIQKINV
HHHHHHHHHHHHHHHHCCCCCHHEEHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ASTIYDIAKHAGVSKSTVSRVLNNQANISEESRKKVLEAIDELNYQPSKLARALTSSGF
CCHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCC
DAIMVISNRSTTTTTGNPFFSEIIQSISTQAELENFDLILQTAKNSEDELKKCLSKIQEK
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHH
MIKGIIMLSSPADEDFFHQLDPYNIPIVVTGKVEGHYKNIYSVDTDNFGDSYALTKHLIK
HHHCEEEECCCCCHHHHHHCCCCCCCEEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHH
QGHQKIACIHAPLDYHVSIDRLAGFRSCLFDHQLDLRNDWIIDSGYSIEDSYKAALRLME
CCCCEEEEEECCCCCEEEHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHC
GPDKPTAVFATDDLKVLSIYKMAADKNLQIPADFSVIGYNDKVASSFLSPPLTSIDIPIN
CCCCCEEEEEECCCEEHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHCCCCCCEECCCHH
KLGKKATNLLFRLIHQDKNVPKTTIIKTEMIERESIQKINV
HHHHHHHHHHHHHHHHCCCCCHHEEHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]