Click here to switch to the map view.

The map label for this gene is recQ [H]

Identifier: 226225309

GI number: 226225309

Start: 2808540

End: 2810312

Strand: Reverse

Name: recQ [H]

Synonym: Lm4b_02732

Alternate gene names: 226225309

Gene position: 2810312-2808540 (Counterclockwise)

Preceding gene: 226225310

Following gene: 226225308

Centisome position: 96.49

GC content: 41.4

Gene sequence:

>1773_bases
ATGATAGAGCAAGCAAGAGCTATTTTACAGCAAAACTTTGGTTATCAAGATTTTCGGAATGGACAAGTTGACGTCATTTC
GAAACTTTGCGCGGGAGAAGATACGCTAGCAATCATGCCAACGGGCGGCGGGAAGTCACTTTGTTACCAAATCCCAGCAC
TTCTTTTTGACGGTTTGACCATTGTTGTTTCTCCACTAATTTCACTTATGAAGGATCAGGTAGATGCGCTAGTCTCAGAG
GGGATTGCAGCTACTTTTATTAATAGTACACTGACTAATAGAGAAATAGATATTCGACTGGATGCTGCATTTTCAGGTGA
GTTAAAGATGCTTTACATTGCTCCAGAACGCATTGAAACGCCAGGTTTTCAGCGTTTAATTGAGCAGGTACCTATTTCAT
TATTTGCGATAGATGAGGCGCACTGTATTTCGCAGTGGGGTCATGATTTTCGCCCTAGCTACCTGACGCTGTGCGATAGT
TTGGATAAAATGACCAGACGTCCGCTCGTTATCGCGCTTACTGCGACAGCGACGCAGGCTGTTTCAGACGATATTTGCCG
ATTGCTAAAAATAGGAGCAGATTCAGTCGTTAAAACTGGATTTTCCAGAGATAATTTGGCTTTTCAAGTAGTGAAAGGGC
AAGATAAAGATAAGTATTTGATTGATTATTTAACAAAAAATGTAACGGAATCCGGAATTATTTATGCTTCGACTCGGAAA
GAAGTAGAGCGGCTGCACACTTTCTTGCTAAAAAAAGGTGTTGAATCTGGTATGTATCATGGCGGAATGACCGATTTAGC
ACGGAAAGACTGGCAAGAGAAGTTTCTATATGATGATATTCGAGTGATTGTGGCGACGAACGCGTTTGGAATGGGAATTA
ATAAATCTAATGTGCGTTTTGTTATTCATTATAATATTCCGCGCAATATTGAAGCGTACTATCAAGAGGCGGGTCGTGCT
GGGCGAGACGGGGTTCCAAGTGATTGTATCTTGTTGTTTTCCCCGCAAGATAGCCGCATCCAGCAGTTTTTAATTGAACA
ATCCGAAATGGACGATGAGCGCAAACAAAATGAATTCGCCAAGCTACGCCAAATGACAGGGTATGGTTATACGGAAATTT
GTTTACAGAAATACATCGTGCAGTATTTTGGAGACGACGAGGAAAACTGCGAGAAATGTAGTAATTGCTTAGATACAAGG
GAAGCCACGGACGTTACAATTTTAGCGCAACAAGTTTTTTCTTGTATAAAAAGAATGGGTGAGCGTTTTGGGAAAGTATT
AATTGCCAAGGTTTTGACAGGTTCTGCTGACCAGAAAGTGAAAGATTGGCGCTTTGACGAACTCAGTACTTATGGATTGA
TGAAAGATGCTTCCCAAAAAGATGTGTTACAACTAATTGATTACCTTACAGCAGAAAAATATTTACAACCAACTGATAGC
CAATTTCCTTCCTTAAAACTGACGGATAGAGCCGTTTCGGTTTTACGAGGTGAGCTAAAAGTGGAAAGAAAACAAGCGAA
ACGGGCTGAAAAAGTTAAAATAGATGTAAATAGTGACCTTTTCGAGAAGTTGCGGGAAGTGCGGCGGGAACTGGCAGCAA
AACACAAGGTGCCGCCATATATCATTTTTTCAGATGAAACGTTACGTGAAATGTGTGCATATATGCCGCAAACGGAAGAT
GCACTGCTTGAAGTCAAAGGCATTGGTGCGATGAAACGCGATAAATACGGTGCAGAATTCTTGGCTGTACTGCAACAAGA
GGCATCGAAATAA

Upstream 100 bases:

>100_bases
AATAAGAAAGCTGCGAGAAAACACGAATTTCTCCAAAAAGCTTCAATTAAAGTCATAAAACCGCTATACTGTTTCTATAG
AGGAGGCGTGAAAAAAGATA

Downstream 100 bases:

>100_bases
AATAGAAGAGAGGGTTTTGACAGATGACAAAAACATTAGTACTGGCAGAGAAACCGTCTGTCGGTAAAGATATCGGCCGG
GTACTCGGGGCTAAACAAGG

Product: ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 590; Mature: 590

Protein sequence:

>590_residues
MIEQARAILQQNFGYQDFRNGQVDVISKLCAGEDTLAIMPTGGGKSLCYQIPALLFDGLTIVVSPLISLMKDQVDALVSE
GIAATFINSTLTNREIDIRLDAAFSGELKMLYIAPERIETPGFQRLIEQVPISLFAIDEAHCISQWGHDFRPSYLTLCDS
LDKMTRRPLVIALTATATQAVSDDICRLLKIGADSVVKTGFSRDNLAFQVVKGQDKDKYLIDYLTKNVTESGIIYASTRK
EVERLHTFLLKKGVESGMYHGGMTDLARKDWQEKFLYDDIRVIVATNAFGMGINKSNVRFVIHYNIPRNIEAYYQEAGRA
GRDGVPSDCILLFSPQDSRIQQFLIEQSEMDDERKQNEFAKLRQMTGYGYTEICLQKYIVQYFGDDEENCEKCSNCLDTR
EATDVTILAQQVFSCIKRMGERFGKVLIAKVLTGSADQKVKDWRFDELSTYGLMKDASQKDVLQLIDYLTAEKYLQPTDS
QFPSLKLTDRAVSVLRGELKVERKQAKRAEKVKIDVNSDLFEKLREVRRELAAKHKVPPYIIFSDETLREMCAYMPQTED
ALLEVKGIGAMKRDKYGAEFLAVLQQEASK

Sequences:

>Translated_590_residues
MIEQARAILQQNFGYQDFRNGQVDVISKLCAGEDTLAIMPTGGGKSLCYQIPALLFDGLTIVVSPLISLMKDQVDALVSE
GIAATFINSTLTNREIDIRLDAAFSGELKMLYIAPERIETPGFQRLIEQVPISLFAIDEAHCISQWGHDFRPSYLTLCDS
LDKMTRRPLVIALTATATQAVSDDICRLLKIGADSVVKTGFSRDNLAFQVVKGQDKDKYLIDYLTKNVTESGIIYASTRK
EVERLHTFLLKKGVESGMYHGGMTDLARKDWQEKFLYDDIRVIVATNAFGMGINKSNVRFVIHYNIPRNIEAYYQEAGRA
GRDGVPSDCILLFSPQDSRIQQFLIEQSEMDDERKQNEFAKLRQMTGYGYTEICLQKYIVQYFGDDEENCEKCSNCLDTR
EATDVTILAQQVFSCIKRMGERFGKVLIAKVLTGSADQKVKDWRFDELSTYGLMKDASQKDVLQLIDYLTAEKYLQPTDS
QFPSLKLTDRAVSVLRGELKVERKQAKRAEKVKIDVNSDLFEKLREVRRELAAKHKVPPYIIFSDETLREMCAYMPQTED
ALLEVKGIGAMKRDKYGAEFLAVLQQEASK
>Mature_590_residues
MIEQARAILQQNFGYQDFRNGQVDVISKLCAGEDTLAIMPTGGGKSLCYQIPALLFDGLTIVVSPLISLMKDQVDALVSE
GIAATFINSTLTNREIDIRLDAAFSGELKMLYIAPERIETPGFQRLIEQVPISLFAIDEAHCISQWGHDFRPSYLTLCDS
LDKMTRRPLVIALTATATQAVSDDICRLLKIGADSVVKTGFSRDNLAFQVVKGQDKDKYLIDYLTKNVTESGIIYASTRK
EVERLHTFLLKKGVESGMYHGGMTDLARKDWQEKFLYDDIRVIVATNAFGMGINKSNVRFVIHYNIPRNIEAYYQEAGRA
GRDGVPSDCILLFSPQDSRIQQFLIEQSEMDDERKQNEFAKLRQMTGYGYTEICLQKYIVQYFGDDEENCEKCSNCLDTR
EATDVTILAQQVFSCIKRMGERFGKVLIAKVLTGSADQKVKDWRFDELSTYGLMKDASQKDVLQLIDYLTAEKYLQPTDS
QFPSLKLTDRAVSVLRGELKVERKQAKRAEKVKIDVNSDLFEKLREVRRELAAKHKVPPYIIFSDETLREMCAYMPQTED
ALLEVKGIGAMKRDKYGAEFLAVLQQEASK

Specific function: Probable DNA helicase. Required for DNA repair and intramolecular recombination; probably has overlapping function with recS (AC P50729). It probably acts to help generate ss-DNA from ds-DNA breaks [H]

COG id: COG0514

COG function: function code L; Superfamily II DNA helicase

Gene ontology:

Cell location: Cytoplasm, nucleoid. Note=Localized throughout the nucleoid in the presence or absence of DNA double- strand breaks [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HRDC domain [H]

Homologues:

Organism=Homo sapiens, GI4557365, Length=630, Percent_Identity=34.1269841269841, Blast_Score=315, Evalue=9e-86,
Organism=Homo sapiens, GI110735439, Length=490, Percent_Identity=35.5102040816327, Blast_Score=267, Evalue=2e-71,
Organism=Homo sapiens, GI169218196, Length=490, Percent_Identity=35.5102040816327, Blast_Score=267, Evalue=2e-71,
Organism=Homo sapiens, GI148612816, Length=422, Percent_Identity=37.6777251184834, Blast_Score=252, Evalue=8e-67,
Organism=Homo sapiens, GI14591902, Length=411, Percent_Identity=36.7396593673966, Blast_Score=252, Evalue=8e-67,
Organism=Homo sapiens, GI14591904, Length=411, Percent_Identity=36.7396593673966, Blast_Score=252, Evalue=8e-67,
Organism=Homo sapiens, GI51242947, Length=387, Percent_Identity=38.7596899224806, Blast_Score=245, Evalue=9e-65,
Organism=Homo sapiens, GI51242943, Length=387, Percent_Identity=38.7596899224806, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI284005309, Length=357, Percent_Identity=36.4145658263305, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI224593278, Length=333, Percent_Identity=22.8228228228228, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI21071032, Length=116, Percent_Identity=34.4827586206897, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI48994977, Length=593, Percent_Identity=40.6408094435076, Blast_Score=459, Evalue=1e-130,
Organism=Escherichia coli, GI87082221, Length=341, Percent_Identity=20.8211143695015, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI25145561, Length=645, Percent_Identity=31.3178294573643, Blast_Score=294, Evalue=9e-80,
Organism=Caenorhabditis elegans, GI32564293, Length=474, Percent_Identity=36.7088607594937, Blast_Score=281, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI71987997, Length=440, Percent_Identity=35.4545454545455, Blast_Score=264, Evalue=9e-71,
Organism=Caenorhabditis elegans, GI71987993, Length=440, Percent_Identity=35.4545454545455, Blast_Score=263, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI17552866, Length=419, Percent_Identity=36.2768496420048, Blast_Score=245, Evalue=5e-65,
Organism=Saccharomyces cerevisiae, GI6323844, Length=582, Percent_Identity=32.9896907216495, Blast_Score=290, Evalue=4e-79,
Organism=Saccharomyces cerevisiae, GI6324778, Length=244, Percent_Identity=26.6393442622951, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324217, Length=339, Percent_Identity=24.4837758112094, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320224, Length=337, Percent_Identity=21.9584569732938, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24646066, Length=636, Percent_Identity=34.2767295597484, Blast_Score=307, Evalue=1e-83,
Organism=Drosophila melanogaster, GI24664226, Length=431, Percent_Identity=36.1948955916473, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI62472181, Length=431, Percent_Identity=35.9628770301624, Blast_Score=254, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24664230, Length=431, Percent_Identity=35.9628770301624, Blast_Score=254, Evalue=1e-67,
Organism=Drosophila melanogaster, GI21358123, Length=353, Percent_Identity=39.3767705382436, Blast_Score=234, Evalue=2e-61,
Organism=Drosophila melanogaster, GI17977678, Length=327, Percent_Identity=26.2996941896024, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356161, Length=359, Percent_Identity=24.5125348189415, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004589
- InterPro:   IPR006293
- InterPro:   IPR002121
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR010997
- InterPro:   IPR018982 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C; PF00570 HRDC; PF09382 RQC [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 66806; Mature: 66806

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS50967 HRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEQARAILQQNFGYQDFRNGQVDVISKLCAGEDTLAIMPTGGGKSLCYQIPALLFDGLT
CCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCHH
IVVSPLISLMKDQVDALVSEGIAATFINSTLTNREIDIRLDAAFSGELKMLYIAPERIET
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEEEEEECCCEEEEEECCHHCCC
PGFQRLIEQVPISLFAIDEAHCISQWGHDFRPSYLTLCDSLDKMTRRPLVIALTATATQA
CHHHHHHHHCCCEEEEECHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEEEEEECHHHHH
VSDDICRLLKIGADSVVKTGFSRDNLAFQVVKGQDKDKYLIDYLTKNVTESGIIYASTRK
HHHHHHHHHHHCCHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCEEEEHHHH
EVERLHTFLLKKGVESGMYHGGMTDLARKDWQEKFLYDDIRVIVATNAFGMGINKSNVRF
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCEEE
VIHYNIPRNIEAYYQEAGRAGRDGVPSDCILLFSPQDSRIQQFLIEQSEMDDERKQNEFA
EEEECCCCCHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHH
KLRQMTGYGYTEICLQKYIVQYFGDDEENCEKCSNCLDTREATDVTILAQQVFSCIKRMG
HHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
ERFGKVLIAKVLTGSADQKVKDWRFDELSTYGLMKDASQKDVLQLIDYLTAEKYLQPTDS
HHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCC
QFPSLKLTDRAVSVLRGELKVERKQAKRAEKVKIDVNSDLFEKLREVRRELAAKHKVPPY
CCCCEEEHHHHHHHHHHHHHHHHHHHCCHHEEEEECCHHHHHHHHHHHHHHHHHCCCCCE
IIFSDETLREMCAYMPQTEDALLEVKGIGAMKRDKYGAEFLAVLQQEASK
EEECCHHHHHHHHHCCCCCHHHEEECCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIEQARAILQQNFGYQDFRNGQVDVISKLCAGEDTLAIMPTGGGKSLCYQIPALLFDGLT
CCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCHH
IVVSPLISLMKDQVDALVSEGIAATFINSTLTNREIDIRLDAAFSGELKMLYIAPERIET
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEEEEEECCCEEEEEECCHHCCC
PGFQRLIEQVPISLFAIDEAHCISQWGHDFRPSYLTLCDSLDKMTRRPLVIALTATATQA
CHHHHHHHHCCCEEEEECHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEEEEEECHHHHH
VSDDICRLLKIGADSVVKTGFSRDNLAFQVVKGQDKDKYLIDYLTKNVTESGIIYASTRK
HHHHHHHHHHHCCHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCEEEEHHHH
EVERLHTFLLKKGVESGMYHGGMTDLARKDWQEKFLYDDIRVIVATNAFGMGINKSNVRF
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCEEE
VIHYNIPRNIEAYYQEAGRAGRDGVPSDCILLFSPQDSRIQQFLIEQSEMDDERKQNEFA
EEEECCCCCHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHH
KLRQMTGYGYTEICLQKYIVQYFGDDEENCEKCSNCLDTREATDVTILAQQVFSCIKRMG
HHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
ERFGKVLIAKVLTGSADQKVKDWRFDELSTYGLMKDASQKDVLQLIDYLTAEKYLQPTDS
HHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCC
QFPSLKLTDRAVSVLRGELKVERKQAKRAEKVKIDVNSDLFEKLREVRRELAAKHKVPPY
CCCCEEEHHHHHHHHHHHHHHHHHHHCCHHEEEEECCHHHHHHHHHHHHHHHHHCCCCCE
IIFSDETLREMCAYMPQTEDALLEVKGIGAMKRDKYGAEFLAVLQQEASK
EEECCHHHHHHHHHCCCCCHHHEEECCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 9642195 [H]