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The map label for this gene is mrpA [H]

Identifier: 226224930

GI number: 226224930

Start: 2426906

End: 2429314

Strand: Direct

Name: mrpA [H]

Synonym: Lm4b_02350

Alternate gene names: 226224930

Gene position: 2426906-2429314 (Clockwise)

Preceding gene: 226224929

Following gene: 226224931

Centisome position: 83.32

GC content: 39.89

Gene sequence:

>2409_bases
TTGTCATTTCTTCATCTAGCAATTGTTCTGCCATTTATTGTGGCACTGCTAATTCCACTTTTTTACCGGTGGACCAAACA
AGTTCATACTGGCTGGTTAGTACTTCCAATTCCAGTGATTTTGTTTATTTACTTCTTAACATTTATCCCAAAAACGATGG
ACGGGGCAACGATTGTTTCAATGCCTTGGATTCCGCGACTTGGGATTAATTTTACGGTAGTAGTAGATGGGCTTAGTTTG
CTGTTTGCGCTACTTATTACGGGGATTGGTTCACTCGTTACCTTTTACTCTATTTATTATTTAGGGAAAAAGAAAGAACG
ACTTAGCAATTTCTATACATTTTTATTTATTTTTATGACGGCAATGCTCGGGGTTGTCTTGAGTGATAACTTGATTGTGT
TGTATCTTTTCTGGGAGCTTACTTCCATTAGTTCGTTCTTATTGATTGGGTATTGGTATCATCGTGAGCGTTCACGGTAC
GGGGCTCGTAAATCGATGATGATTACAGTCTTCGGCGGACTGATGATGCTTGGCGGATTTATTTTGCTTCATATTATGAG
CGATTCTTTTTCCATTCGGGAGATTATTAGTAACGCGGATGTAATTAGTAATAATAGTTTATTCATTCCTGCGATGATTC
TTATCTTGCTCGGGGCATTTACGAAATCTGCGCAAGTTCCATTCCATATTTGGTTGCCAGATGCGATGGAAGCTCCTACT
CCGGTCAGTGCCTACCTCCACTCTGCTACGATGGTAAAAGCTGGGATTTATATTGTCGCTCGGTTCACGCCACTATTTGC
AAGTTCGGGTGTATGGTTTTGGACGGTATCACTCGTTGGGATTACGACCCTTTTCTGGGGTTCACTTAATGCCACGAAGA
AAAATGATTTAAAAGCAATTTTGGCTTATTCGACGATAAGTCAGCTTGGTCTCATTATGGCGCTTCTTGGTGTCGGTGCT
GCCTCCCTTCACTTTGACACGCTTAGTGATGATATTTATGTGATGGCGATTGTCGCAGCTGTGTTCCATCTATTTAATCA
CGCGACATTTAAAGGTAGTTTGTTTATGATGGTTGGGATTGTCGATCATGAAACAGGGACGCGGGATATTAGGCGACTTG
GTGGCTTAATGCGAATTATGCCGATTACGGCGACGATTGCGTTTATCGGAACGTTCGCAATGGCCGGTATTCCACCGTTT
AATGGATTTCTAAGTAAAGAGATGTTTTTTGAAAGCATGGTAAATATTACGCAATTGCAATTGTTTGATGCAAGTACTTG
GGGCGTTCTGCTCCCTGTTGTAGCTTGGGTTGCTAGTGTGTTTACGTTTGTTTATAGTATGATTATTTTCTTTAAAACAT
TTACTGGAAAAGTGAAACCGTACTTACTTCCGAAAAAACCGCATGAAGCGCCATTCGGACTACTTTTACCACCGATTATT
TTATCGATTTTCGTGGTTGGTATTGGTCTCTTCCCTAATTTAATTGCGGAGCCGATTTTGGAGCCTGCTGTAAGAGCAAT
TGTTCCTGGGCTTTCTACGGATTTCTCGATTCATATTAGTTTGTGGCACGGCTTTACGCCTGCTCTGTTAATGACGTTCG
GTGTTGTTGCAGTGGGGATTGTCTTGTTTCTTACACATAAATACTGGAAACCTTGGATTACGACTAAGGTTCCAAAAGCA
CTTCGAATTGGGAAAACGTATGATAATGGGATGTATTATTTAGAACAAGGCTCGTATCGTATGACAATGTTTATTATGAC
TGGTTGGCTTCGGACTTACTTGAATTACATGTTATCGGCTTTTATTTTAATGATGGCTTCGGTCATGATTTTCACACAAG
CACTTGATTTCAATTTCTCAAGTATGACGAAAGTGACAGTTGTTGATTTTGTTTTAGCGGCAGTGATTTTAGTGACGTTG
GTTGGGATTGTCTTTTCCAAATCGCGGATTACTTCGATTATTTTACTTGGAGCAATGGGATACACGATTAGTATTTTCTT
TGTTATCTCTAGAGCACCAGACCTTGCTTTAACACAGCTTATTATTGAAACTATTTCCGTTGTGCTTTATCTACTTGTGT
TTTATCATCTTCCTCAGTTTAGTAATATTGAGGAAAAGCCGAAATGGTTATCAATGAAGACATTTTTGAGTATCGGGGTT
GGTGTAATTATTACCCTTGTCTCTCTTTCAGCGTATAATACGACATTTTATGATTCGATTTCGAAGTACTATGTGGACAA
TGCTTATGTGGAAGCGGCGGGACGAAATATTGTAAATGTTATCTTAGTTGACTTCCGTGGTTTTGATACGATGTTTGAGA
CAGCTGTGCTTTCGATTGCGGCGATTGGTATTTACGCGATGATTAAATTACGACTGACGAAACGAGGTGAGAATGATGAA
AACGAATGA

Upstream 100 bases:

>100_bases
TAAATGTAAGTACTTGCTTAAAAAATAATGATTTCCTGTTTTTATTTTCACATTTCCAGGATGAGTTTCCGTATAAAAAT
TCTTCGAGGAGGAGCCGATT

Downstream 100 bases:

>100_bases
TGTTCTACTGAGAAATGTAACGAAAGTAGTTGCTTTCATTATTTTCTTATTCTCACTCCATTTATTCTTTGCAGGACATT
ATAATCCTGGTGGCGGGTTT

Product: monovalent cation/H+ antiporter subunit A

Products: NAD+; ubiquinol [C]

Alternate protein names: Mrp complex subunit A; Multiple resistance and pH homeostasis protein A [H]

Number of amino acids: Translated: 802; Mature: 801

Protein sequence:

>802_residues
MSFLHLAIVLPFIVALLIPLFYRWTKQVHTGWLVLPIPVILFIYFLTFIPKTMDGATIVSMPWIPRLGINFTVVVDGLSL
LFALLITGIGSLVTFYSIYYLGKKKERLSNFYTFLFIFMTAMLGVVLSDNLIVLYLFWELTSISSFLLIGYWYHRERSRY
GARKSMMITVFGGLMMLGGFILLHIMSDSFSIREIISNADVISNNSLFIPAMILILLGAFTKSAQVPFHIWLPDAMEAPT
PVSAYLHSATMVKAGIYIVARFTPLFASSGVWFWTVSLVGITTLFWGSLNATKKNDLKAILAYSTISQLGLIMALLGVGA
ASLHFDTLSDDIYVMAIVAAVFHLFNHATFKGSLFMMVGIVDHETGTRDIRRLGGLMRIMPITATIAFIGTFAMAGIPPF
NGFLSKEMFFESMVNITQLQLFDASTWGVLLPVVAWVASVFTFVYSMIIFFKTFTGKVKPYLLPKKPHEAPFGLLLPPII
LSIFVVGIGLFPNLIAEPILEPAVRAIVPGLSTDFSIHISLWHGFTPALLMTFGVVAVGIVLFLTHKYWKPWITTKVPKA
LRIGKTYDNGMYYLEQGSYRMTMFIMTGWLRTYLNYMLSAFILMMASVMIFTQALDFNFSSMTKVTVVDFVLAAVILVTL
VGIVFSKSRITSIILLGAMGYTISIFFVISRAPDLALTQLIIETISVVLYLLVFYHLPQFSNIEEKPKWLSMKTFLSIGV
GVIITLVSLSAYNTTFYDSISKYYVDNAYVEAAGRNIVNVILVDFRGFDTMFETAVLSIAAIGIYAMIKLRLTKRGENDE
NE

Sequences:

>Translated_802_residues
MSFLHLAIVLPFIVALLIPLFYRWTKQVHTGWLVLPIPVILFIYFLTFIPKTMDGATIVSMPWIPRLGINFTVVVDGLSL
LFALLITGIGSLVTFYSIYYLGKKKERLSNFYTFLFIFMTAMLGVVLSDNLIVLYLFWELTSISSFLLIGYWYHRERSRY
GARKSMMITVFGGLMMLGGFILLHIMSDSFSIREIISNADVISNNSLFIPAMILILLGAFTKSAQVPFHIWLPDAMEAPT
PVSAYLHSATMVKAGIYIVARFTPLFASSGVWFWTVSLVGITTLFWGSLNATKKNDLKAILAYSTISQLGLIMALLGVGA
ASLHFDTLSDDIYVMAIVAAVFHLFNHATFKGSLFMMVGIVDHETGTRDIRRLGGLMRIMPITATIAFIGTFAMAGIPPF
NGFLSKEMFFESMVNITQLQLFDASTWGVLLPVVAWVASVFTFVYSMIIFFKTFTGKVKPYLLPKKPHEAPFGLLLPPII
LSIFVVGIGLFPNLIAEPILEPAVRAIVPGLSTDFSIHISLWHGFTPALLMTFGVVAVGIVLFLTHKYWKPWITTKVPKA
LRIGKTYDNGMYYLEQGSYRMTMFIMTGWLRTYLNYMLSAFILMMASVMIFTQALDFNFSSMTKVTVVDFVLAAVILVTL
VGIVFSKSRITSIILLGAMGYTISIFFVISRAPDLALTQLIIETISVVLYLLVFYHLPQFSNIEEKPKWLSMKTFLSIGV
GVIITLVSLSAYNTTFYDSISKYYVDNAYVEAAGRNIVNVILVDFRGFDTMFETAVLSIAAIGIYAMIKLRLTKRGENDE
NE
>Mature_801_residues
SFLHLAIVLPFIVALLIPLFYRWTKQVHTGWLVLPIPVILFIYFLTFIPKTMDGATIVSMPWIPRLGINFTVVVDGLSLL
FALLITGIGSLVTFYSIYYLGKKKERLSNFYTFLFIFMTAMLGVVLSDNLIVLYLFWELTSISSFLLIGYWYHRERSRYG
ARKSMMITVFGGLMMLGGFILLHIMSDSFSIREIISNADVISNNSLFIPAMILILLGAFTKSAQVPFHIWLPDAMEAPTP
VSAYLHSATMVKAGIYIVARFTPLFASSGVWFWTVSLVGITTLFWGSLNATKKNDLKAILAYSTISQLGLIMALLGVGAA
SLHFDTLSDDIYVMAIVAAVFHLFNHATFKGSLFMMVGIVDHETGTRDIRRLGGLMRIMPITATIAFIGTFAMAGIPPFN
GFLSKEMFFESMVNITQLQLFDASTWGVLLPVVAWVASVFTFVYSMIIFFKTFTGKVKPYLLPKKPHEAPFGLLLPPIIL
SIFVVGIGLFPNLIAEPILEPAVRAIVPGLSTDFSIHISLWHGFTPALLMTFGVVAVGIVLFLTHKYWKPWITTKVPKAL
RIGKTYDNGMYYLEQGSYRMTMFIMTGWLRTYLNYMLSAFILMMASVMIFTQALDFNFSSMTKVTVVDFVLAAVILVTLV
GIVFSKSRITSIILLGAMGYTISIFFVISRAPDLALTQLIIETISVVLYLLVFYHLPQFSNIEEKPKWLSMKTFLSIGVG
VIITLVSLSAYNTTFYDSISKYYVDNAYVEAAGRNIVNVILVDFRGFDTMFETAVLSIAAIGIYAMIKLRLTKRGENDEN
E

Specific function: Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual N

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit A family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=368, Percent_Identity=35.3260869565217, Blast_Score=190, Evalue=4e-48,
Organism=Escherichia coli, GI1788614, Length=562, Percent_Identity=31.4946619217082, Blast_Score=206, Evalue=7e-54,
Organism=Escherichia coli, GI1788829, Length=416, Percent_Identity=33.6538461538462, Blast_Score=179, Evalue=8e-46,
Organism=Escherichia coli, GI1788831, Length=352, Percent_Identity=33.2386363636364, Blast_Score=144, Evalue=3e-35,
Organism=Escherichia coli, GI1788827, Length=446, Percent_Identity=27.1300448430493, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI1788613, Length=423, Percent_Identity=26.9503546099291, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI2367154, Length=288, Percent_Identity=29.1666666666667, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI145693160, Length=342, Percent_Identity=26.3157894736842, Blast_Score=87, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005663
- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 89757; Mature: 89626

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFLHLAIVLPFIVALLIPLFYRWTKQVHTGWLVLPIPVILFIYFLTFIPKTMDGATIVS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHCCCCCCCCEEEE
MPWIPRLGINFTVVVDGLSLLFALLITGIGSLVTFYSIYYLGKKKERLSNFYTFLFIFMT
CCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
AMLGVVLSDNLIVLYLFWELTSISSFLLIGYWYHRERSRYGARKSMMITVFGGLMMLGGF
HHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
ILLHIMSDSFSIREIISNADVISNNSLFIPAMILILLGAFTKSAQVPFHIWLPDAMEAPT
HHHHHHCCCHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCC
PVSAYLHSATMVKAGIYIVARFTPLFASSGVWFWTVSLVGITTLFWGSLNATKKNDLKAI
CHHHHHHHHHHHHHCEEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
LAYSTISQLGLIMALLGVGAASLHFDTLSDDIYVMAIVAAVFHLFNHATFKGSLFMMVGI
HHHHHHHHHHHHHHHHHCCHHHEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEE
VDHETGTRDIRRLGGLMRIMPITATIAFIGTFAMAGIPPFNGFLSKEMFFESMVNITQLQ
EECCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHEE
LFDASTWGVLLPVVAWVASVFTFVYSMIIFFKTFTGKVKPYLLPKKPHEAPFGLLLPPII
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCHHHHHHH
LSIFVVGIGLFPNLIAEPILEPAVRAIVPGLSTDFSIHISLWHGFTPALLMTFGVVAVGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHH
VLFLTHKYWKPWITTKVPKALRIGKTYDNGMYYLEQGSYRMTMFIMTGWLRTYLNYMLSA
HHHHHHHHCCCCCCCCCCHHHHCCCEECCCEEEEECCCEEEEHHHHHHHHHHHHHHHHHH
FILMMASVMIFTQALDFNFSSMTKVTVVDFVLAAVILVTLVGIVFSKSRITSIILLGAMG
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YTISIFFVISRAPDLALTQLIIETISVVLYLLVFYHLPQFSNIEEKPKWLSMKTFLSIGV
HHHEEHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
GVIITLVSLSAYNTTFYDSISKYYVDNAYVEAAGRNIVNVILVDFRGFDTMFETAVLSIA
HHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHH
AIGIYAMIKLRLTKRGENDENE
HHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SFLHLAIVLPFIVALLIPLFYRWTKQVHTGWLVLPIPVILFIYFLTFIPKTMDGATIVS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHCCCCCCCCEEEE
MPWIPRLGINFTVVVDGLSLLFALLITGIGSLVTFYSIYYLGKKKERLSNFYTFLFIFMT
CCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
AMLGVVLSDNLIVLYLFWELTSISSFLLIGYWYHRERSRYGARKSMMITVFGGLMMLGGF
HHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
ILLHIMSDSFSIREIISNADVISNNSLFIPAMILILLGAFTKSAQVPFHIWLPDAMEAPT
HHHHHHCCCHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCC
PVSAYLHSATMVKAGIYIVARFTPLFASSGVWFWTVSLVGITTLFWGSLNATKKNDLKAI
CHHHHHHHHHHHHHCEEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
LAYSTISQLGLIMALLGVGAASLHFDTLSDDIYVMAIVAAVFHLFNHATFKGSLFMMVGI
HHHHHHHHHHHHHHHHHCCHHHEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEE
VDHETGTRDIRRLGGLMRIMPITATIAFIGTFAMAGIPPFNGFLSKEMFFESMVNITQLQ
EECCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHEE
LFDASTWGVLLPVVAWVASVFTFVYSMIIFFKTFTGKVKPYLLPKKPHEAPFGLLLPPII
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCHHHHHHH
LSIFVVGIGLFPNLIAEPILEPAVRAIVPGLSTDFSIHISLWHGFTPALLMTFGVVAVGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHH
VLFLTHKYWKPWITTKVPKALRIGKTYDNGMYYLEQGSYRMTMFIMTGWLRTYLNYMLSA
HHHHHHHHCCCCCCCCCCHHHHCCCEECCCEEEEECCCEEEEHHHHHHHHHHHHHHHHHH
FILMMASVMIFTQALDFNFSSMTKVTVVDFVLAAVILVTLVGIVFSKSRITSIILLGAMG
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YTISIFFVISRAPDLALTQLIIETISVVLYLLVFYHLPQFSNIEEKPKWLSMKTFLSIGV
HHHEEHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
GVIITLVSLSAYNTTFYDSISKYYVDNAYVEAAGRNIVNVILVDFRGFDTMFETAVLSIA
HHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHH
AIGIYAMIKLRLTKRGENDENE
HHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 10198001; 11356194 [H]