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The map label for this gene is gadC [H]

Identifier: 226224913

GI number: 226224913

Start: 2402027

End: 2403550

Strand: Direct

Name: gadC [H]

Synonym: Lm4b_02332

Alternate gene names: 226224913

Gene position: 2402027-2403550 (Clockwise)

Preceding gene: 226224897

Following gene: 226224914

Centisome position: 82.47

GC content: 40.94

Gene sequence:

>1524_bases
ATGTCTAAACAAGCAAAGTCTTTAACCTTATTCGGATTTTTCGCCATTACGGCTTCGATGGTTATGACAGTTTATGAATA
TCCAACATTTGCCACTTCCGGCTTTCATCTCGTATTTTTCTTGCTACTTGGCGGATTTTTATGGTTCTTACCTGTCGCTT
TATGTGCAGCAGAAATGGCGACAGTTGATGGCTGGCAAGAAGGCGGTATTTTTTCTTGGGTGGGAAATACACTCGGGGAA
CGTTTTGGTTTTGCCGCAATCTTCTTTCAGTGGTTTCAAATAACGGTCGGATTCGTGACAATGATTTACTTTATTCTTGG
CGCGCTATCGTATGTATTTGATTTTCCCGCGCTCGAATCGAACCCTTTTATTAAATTTATCGGTGTTTTAATCATTTTCT
GGGGACTTACTTTTTCTCAACTAGGTGGTACGAAAAACACTGCTAAAATCGCGAAATTTGGTTTTATTATCGGTATTCTC
ATCCCAGCAATCATTTTATTTATCCTAGGTATTGCTTATGTTGCTGGCGGAAATCCCCTTCATGTCACTTTTTCCAAAGA
GGCGTTTATCCCGGATTTCTCAAAAGCTTCAACATTAGTCATCTTCGTGTCATTTATACTCGCTTACATGGGTGTTGAAG
CTTCCGCGAGCCACGTAAATGAAATGACCAATCCAAAACGCGATTATCCACTAGCGATGATTATGCTCGTTATTTTAGCC
ATCGCACTGAATACAATCGGCGGCCTAACCATTTCCGCTGTGTTACCTTTAAAAGATTTATCCCTTAGCTCCGGTGTCGT
CCAAACATTCCAAGCATTAATTCTGCACTTTGGTTCCGGCTTAGGATGGGCAGTAAAACTGATTGCCATCATGATTGCAC
TTGGCGTCATGGGTGAAGTCAGTGCCTGGGTAGTCGGCCCCTCACGCGGAATGTATACTGCAGCAGAACAAGGCTTACTT
CCAGAAAAAATGAAAAAAGTAAATAAACACGGTGTCCCTGTTCCACTTATTATGGTTCAAGGCGTCGTCGTTACCATTTG
GGCAGCTATTCTCACATTCGGCGGCGGCGGAAATAATTTATCATTCCTAACTGCCATTTCCTTAACCGTAGTCATTTATT
TAGTCGGCTATTTACTCTTTTTCATCGGCTACTTAGTACTTATTTTCAAAAAATCAAGCTTAAAACGCACCTATCAAATC
CCTGGTGGCAAAATTGTTAAAGTGATTGTAGCGCTAATCGGCTTAGTAACATCCGTTTTCGCTTTATGTATCTCTTTTGT
TCCACCAGCATCCATCGCCGCAAAAAGCGACACGACATACATGGTTATCTTATCAATCAGCTTTATCGTCACCGTACTAA
TTCCATTTATCATCTACGCTGTGCACGATAAAAAAGACATCTCACCGAAAGATATTACACATTTAGAATCAAGCGACATC
AATAAATTCACCCACCCAAGAGCTCGTGGCGAACACATTATTAACCCCGACGAAAAACATATTATGAATCAAGACAAATT
ATAG

Upstream 100 bases:

>100_bases
CCAAAAAAATCACATTTACCATTAATTAAGCATCATTCTAATTACTTATAGCTTCGTTTAGTTGTATTTTCGCAAACAAA
AAATTGGGAGGTACTACACA

Downstream 100 bases:

>100_bases
GAGGAAAAAATCATGTTATATAGTAAAGAAAATAAAGAAAGTTATTTAGAACCAGTTTTCGGATCAAGTGCAGAGGATCG
TGATATTCCTAAATACACCC

Product: hypothetical protein

Products: L-glutamate [Cytoplasm]; 4-aminobutyrate [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MSKQAKSLTLFGFFAITASMVMTVYEYPTFATSGFHLVFFLLLGGFLWFLPVALCAAEMATVDGWQEGGIFSWVGNTLGE
RFGFAAIFFQWFQITVGFVTMIYFILGALSYVFDFPALESNPFIKFIGVLIIFWGLTFSQLGGTKNTAKIAKFGFIIGIL
IPAIILFILGIAYVAGGNPLHVTFSKEAFIPDFSKASTLVIFVSFILAYMGVEASASHVNEMTNPKRDYPLAMIMLVILA
IALNTIGGLTISAVLPLKDLSLSSGVVQTFQALILHFGSGLGWAVKLIAIMIALGVMGEVSAWVVGPSRGMYTAAEQGLL
PEKMKKVNKHGVPVPLIMVQGVVVTIWAAILTFGGGGNNLSFLTAISLTVVIYLVGYLLFFIGYLVLIFKKSSLKRTYQI
PGGKIVKVIVALIGLVTSVFALCISFVPPASIAAKSDTTYMVILSISFIVTVLIPFIIYAVHDKKDISPKDITHLESSDI
NKFTHPRARGEHIINPDEKHIMNQDKL

Sequences:

>Translated_507_residues
MSKQAKSLTLFGFFAITASMVMTVYEYPTFATSGFHLVFFLLLGGFLWFLPVALCAAEMATVDGWQEGGIFSWVGNTLGE
RFGFAAIFFQWFQITVGFVTMIYFILGALSYVFDFPALESNPFIKFIGVLIIFWGLTFSQLGGTKNTAKIAKFGFIIGIL
IPAIILFILGIAYVAGGNPLHVTFSKEAFIPDFSKASTLVIFVSFILAYMGVEASASHVNEMTNPKRDYPLAMIMLVILA
IALNTIGGLTISAVLPLKDLSLSSGVVQTFQALILHFGSGLGWAVKLIAIMIALGVMGEVSAWVVGPSRGMYTAAEQGLL
PEKMKKVNKHGVPVPLIMVQGVVVTIWAAILTFGGGGNNLSFLTAISLTVVIYLVGYLLFFIGYLVLIFKKSSLKRTYQI
PGGKIVKVIVALIGLVTSVFALCISFVPPASIAAKSDTTYMVILSISFIVTVLIPFIIYAVHDKKDISPKDITHLESSDI
NKFTHPRARGEHIINPDEKHIMNQDKL
>Mature_506_residues
SKQAKSLTLFGFFAITASMVMTVYEYPTFATSGFHLVFFLLLGGFLWFLPVALCAAEMATVDGWQEGGIFSWVGNTLGER
FGFAAIFFQWFQITVGFVTMIYFILGALSYVFDFPALESNPFIKFIGVLIIFWGLTFSQLGGTKNTAKIAKFGFIIGILI
PAIILFILGIAYVAGGNPLHVTFSKEAFIPDFSKASTLVIFVSFILAYMGVEASASHVNEMTNPKRDYPLAMIMLVILAI
ALNTIGGLTISAVLPLKDLSLSSGVVQTFQALILHFGSGLGWAVKLIAIMIALGVMGEVSAWVVGPSRGMYTAAEQGLLP
EKMKKVNKHGVPVPLIMVQGVVVTIWAAILTFGGGGNNLSFLTAISLTVVIYLVGYLLFFIGYLVLIFKKSSLKRTYQIP
GGKIVKVIVALIGLVTSVFALCISFVPPASIAAKSDTTYMVILSISFIVTVLIPFIIYAVHDKKDISPKDITHLESSDIN
KFTHPRARGEHIINPDEKHIMNQDKL

Specific function: Involved in glutamate-dependent acid resistance. May be involved in antiport of glutamate and glutamate-gamma- aminobutyrate (GABA) [H]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Glutamate:GABA antiporter (GGA) (TC 2.A.3.7) family [H]

Homologues:

Organism=Escherichia coli, GI1787768, Length=505, Percent_Identity=56.039603960396, Blast_Score=568, Evalue=1e-163,
Organism=Escherichia coli, GI48994921, Length=384, Percent_Identity=22.3958333333333, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI87082380, Length=485, Percent_Identity=23.0927835051546, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI87081798, Length=438, Percent_Identity=21.0045662100457, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI2367353, Length=299, Percent_Identity=22.4080267558528, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004759 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 55169; Mature: 55037

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCCCCCCCCCCCCHHHHCCCCCC
>Mature Secondary Structure 
SKQAKSLTLFGFFAITASMVMTVYEYPTFATSGFHLVFFLLLGGFLWFLPVALCAAEMA
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVDGWQEGGIFSWVGNTLGERFGFAAIFFQWFQITVGFVTMIYFILGALSYVFDFPALES
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NPFIKFIGVLIIFWGLTFSQLGGTKNTAKIAKFGFIIGILIPAIILFILGIAYVAGGNPL
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
HVTFSKEAFIPDFSKASTLVIFVSFILAYMGVEASASHVNEMTNPKRDYPLAMIMLVILA
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHH
IALNTIGGLTISAVLPLKDLSLSSGVVQTFQALILHFGSGLGWAVKLIAIMIALGVMGEV
HHHHHHCCCHHHHCCCHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
SAWVVGPSRGMYTAAEQGLLPEKMKKVNKHGVPVPLIMVQGVVVTIWAAILTFGGGGNNL
CEEEECCCCCCHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCH
SFLTAISLTVVIYLVGYLLFFIGYLVLIFKKSSLKRTYQIPGGKIVKVIVALIGLVTSVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHH
ALCISFVPPASIAAKSDTTYMVILSISFIVTVLIPFIIYAVHDKKDISPKDITHLESSDI
HHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCC
NKFTHPRARGEHIINPDEKHIMNQDKL
HHHCCCCCCCCCCCCCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-aminobutyrate [Cytoplasm]; L-glutamate [Periplasm] [C]

Specific reaction: 4-aminobutyrate [Cytoplasm] + L-glutamate [Periplasm] = L-glutamate [Cytoplasm] + 4-aminobutyrate [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11337471; 10411264 [H]