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The map label for this gene is gadB [H]

Identifier: 226224914

GI number: 226224914

Start: 2403563

End: 2404957

Strand: Direct

Name: gadB [H]

Synonym: Lm4b_02334

Alternate gene names: 226224914

Gene position: 2403563-2404957 (Clockwise)

Preceding gene: 226224913

Following gene: 226224920

Centisome position: 82.52

GC content: 42.65

Gene sequence:

>1395_bases
ATGTTATATAGTAAAGAAAATAAAGAAAGTTATTTAGAACCAGTTTTCGGATCAAGTGCAGAGGATCGTGATATTCCTAA
ATACACCCTCGGAAAAGAACCACTCGAACCTCGGATTGCCTATCGTTTAGTAAAAGACGAACTTTTAGATGAAGGTTCTG
CCCGTCAAAACTTAGCTACTTTTTGCCAAACATATATGGAAGACGAAGCAACCAAATTAATGTCCGAAACACTAGAAAAA
AATGCAATCGATAAATCTGAATACCCAAGAACAGCTGAACTTGAAAATCGTTGCGTCAATATTATCGCTGACTTATGGCA
TGCACCTAAGGACCAAAAATTCATGGGAACTTCCACAATCGGCTCTAGTGAAGCGTGTATGCTGGGCGGAATGGCAATGA
AATTTGCTTGGAGAAAACGTGCGGAAAAACTTGGCTTAGATATTTATGCGCAAAAACCAAACCTCGTTATCTCCTCTGGC
TACCAAGTGTGCTGGGAAAAATTCTGTGTCTACTGGGATATCGATATGCGCGTTGTTCCAATGGATAAAGATCACATGCA
ACTGAATACCGACCAAGTCCTTGATTACGTCGACGAATATACAATCGGCGTTGTCGGCATCCTCGGCATCACTTACACAG
GCCGCTACGATGACATCTACGCACTTAACGAAAAATTAGAAGAATACAATAGCAAAACAGATTATAAAGTCTACATTCAC
GTTGACGCAGCTAGTGGTGGCTTCTTCACTCCATTTGTCGAACCCGATATTATCTGGGACTTCCGCTTGAAAAACGTTAT
CTCCATTAATACTTCTGGTCACAAATACGGCCTCGTTTATCCTGGTATCGGTTGGGTCCTTTGGAAAGATGAAAGCTACC
TACCAGAAGAACTTATTTTTAAAGTCAGCTACCTTGGCGGAGAAATGCCAACCATGCAAATCAATTTCTCCCGCAGTGCG
AGCCACATCATCGGTCAATACTACAATTTCTTACGCTACGGCTTCGAAGGCTACCGCACCATTCATCAAAAAACAAGCGA
TGTAGCACAATACCTTGCCCATGCAGTCGAACAAACCGGCTACTTTGACATTTATAACGACGGCTCCCATTTACCAATCG
TCTGCTATAAATTAAAAGACGACGCTAACGTAAAATGGACATTATATGATTTAGCGGACCGCCTCCAAATGCGCGGCTGG
CAAGTTCCCGCATACCCACTACCAAAAAACTTAGAAAACATCATCATCCAACGTTACGTCTGCCGCGCTGACCTCGGTTT
CAACATGGCAGAAGAATTCATCCAAGATTTCCAAGCCTCTATTCAAGAACTAAACAACGCTCACATCCTCTTCCATGATA
CCCAACAATCCGGCGTCCACGGCTTCACACATTAA

Upstream 100 bases:

>100_bases
CATCAATAAATTCACCCACCCAAGAGCTCGTGGCGAACACATTATTAACCCCGACGAAAAACATATTATGAATCAAGACA
AATTATAGGAGGAAAAAATC

Downstream 100 bases:

>100_bases
TAAAAAGGCTAGCCTCTCAAATAGGCTAGCCTTTTTTCATGTTCTTTTATTCTAAGCGACTGAATCGCCCTTTCTACATC
TACAAGATCTCTGTCAAAAA

Product: glutamate decarboxylase

Products: NA

Alternate protein names: GAD-beta [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MLYSKENKESYLEPVFGSSAEDRDIPKYTLGKEPLEPRIAYRLVKDELLDEGSARQNLATFCQTYMEDEATKLMSETLEK
NAIDKSEYPRTAELENRCVNIIADLWHAPKDQKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIYAQKPNLVISSG
YQVCWEKFCVYWDIDMRVVPMDKDHMQLNTDQVLDYVDEYTIGVVGILGITYTGRYDDIYALNEKLEEYNSKTDYKVYIH
VDAASGGFFTPFVEPDIIWDFRLKNVISINTSGHKYGLVYPGIGWVLWKDESYLPEELIFKVSYLGGEMPTMQINFSRSA
SHIIGQYYNFLRYGFEGYRTIHQKTSDVAQYLAHAVEQTGYFDIYNDGSHLPIVCYKLKDDANVKWTLYDLADRLQMRGW
QVPAYPLPKNLENIIIQRYVCRADLGFNMAEEFIQDFQASIQELNNAHILFHDTQQSGVHGFTH

Sequences:

>Translated_464_residues
MLYSKENKESYLEPVFGSSAEDRDIPKYTLGKEPLEPRIAYRLVKDELLDEGSARQNLATFCQTYMEDEATKLMSETLEK
NAIDKSEYPRTAELENRCVNIIADLWHAPKDQKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIYAQKPNLVISSG
YQVCWEKFCVYWDIDMRVVPMDKDHMQLNTDQVLDYVDEYTIGVVGILGITYTGRYDDIYALNEKLEEYNSKTDYKVYIH
VDAASGGFFTPFVEPDIIWDFRLKNVISINTSGHKYGLVYPGIGWVLWKDESYLPEELIFKVSYLGGEMPTMQINFSRSA
SHIIGQYYNFLRYGFEGYRTIHQKTSDVAQYLAHAVEQTGYFDIYNDGSHLPIVCYKLKDDANVKWTLYDLADRLQMRGW
QVPAYPLPKNLENIIIQRYVCRADLGFNMAEEFIQDFQASIQELNNAHILFHDTQQSGVHGFTH
>Mature_464_residues
MLYSKENKESYLEPVFGSSAEDRDIPKYTLGKEPLEPRIAYRLVKDELLDEGSARQNLATFCQTYMEDEATKLMSETLEK
NAIDKSEYPRTAELENRCVNIIADLWHAPKDQKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIYAQKPNLVISSG
YQVCWEKFCVYWDIDMRVVPMDKDHMQLNTDQVLDYVDEYTIGVVGILGITYTGRYDDIYALNEKLEEYNSKTDYKVYIH
VDAASGGFFTPFVEPDIIWDFRLKNVISINTSGHKYGLVYPGIGWVLWKDESYLPEELIFKVSYLGGEMPTMQINFSRSA
SHIIGQYYNFLRYGFEGYRTIHQKTSDVAQYLAHAVEQTGYFDIYNDGSHLPIVCYKLKDDANVKWTLYDLADRLQMRGW
QVPAYPLPKNLENIIIQRYVCRADLGFNMAEEFIQDFQASIQELNNAHILFHDTQQSGVHGFTH

Specific function: Converts internalized glutamate to GABA and increases the internal pH. Involved in glutamate-dependent acid resistance in gastric fluid [H]

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family [H]

Homologues:

Organism=Homo sapiens, GI31982936, Length=369, Percent_Identity=21.9512195121951, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1789934, Length=460, Percent_Identity=45, Blast_Score=421, Evalue=1e-119,
Organism=Escherichia coli, GI1787769, Length=460, Percent_Identity=45, Blast_Score=420, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17557272, Length=448, Percent_Identity=23.6607142857143, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI25148342, Length=368, Percent_Identity=22.0108695652174, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17543922, Length=385, Percent_Identity=21.2987012987013, Blast_Score=88, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323905, Length=465, Percent_Identity=37.6344086021505, Blast_Score=306, Evalue=5e-84,
Organism=Saccharomyces cerevisiae, GI6320500, Length=373, Percent_Identity=20.3753351206434, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21355963, Length=384, Percent_Identity=24.21875, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24654344, Length=384, Percent_Identity=24.21875, Blast_Score=105, Evalue=9e-23,

Paralogues:

None

Copy number: 3820 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010107
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421 [H]

Pfam domain/function: PF00282 Pyridoxal_deC [H]

EC number: =4.1.1.15 [H]

Molecular weight: Translated: 53586; Mature: 53586

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYSKENKESYLEPVFGSSAEDRDIPKYTLGKEPLEPRIAYRLVKDELLDEGSARQNLAT
CCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHH
FCQTYMEDEATKLMSETLEKNAIDKSEYPRTAELENRCVNIIADLWHAPKDQKFMGTSTI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCC
GSSEACMLGGMAMKFAWRKRAEKLGLDIYAQKPNLVISSGYQVCWEKFCVYWDIDMRVVP
CCCCHHHHCCHHHHHHHHHHHHHCCCEEEECCCCEEEECCHHHHHHCEEEEEECCEEEEE
MDKDHMQLNTDQVLDYVDEYTIGVVGILGITYTGRYDDIYALNEKLEEYNSKTDYKVYIH
CCCCCCEECHHHHHHHHHHHHHHHHEEHHEEECCCCCCHHHHHHHHHHHCCCCCEEEEEE
VDAASGGFFTPFVEPDIIWDFRLKNVISINTSGHKYGLVYPGIGWVLWKDESYLPEELIF
EEECCCCEECCCCCCCEEEEEEECEEEEECCCCCEEEEEECCCEEEEECCCCCCCHHHHH
KVSYLGGEMPTMQINFSRSASHIIGQYYNFLRYGFEGYRTIHQKTSDVAQYLAHAVEQTG
HHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
YFDIYNDGSHLPIVCYKLKDDANVKWTLYDLADRLQMRGWQVPAYPLPKNLENIIIQRYV
CEEEECCCCCCCEEEEEECCCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
CRADLGFNMAEEFIQDFQASIQELNNAHILFHDTQQSGVHGFTH
HHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MLYSKENKESYLEPVFGSSAEDRDIPKYTLGKEPLEPRIAYRLVKDELLDEGSARQNLAT
CCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHH
FCQTYMEDEATKLMSETLEKNAIDKSEYPRTAELENRCVNIIADLWHAPKDQKFMGTSTI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCC
GSSEACMLGGMAMKFAWRKRAEKLGLDIYAQKPNLVISSGYQVCWEKFCVYWDIDMRVVP
CCCCHHHHCCHHHHHHHHHHHHHCCCEEEECCCCEEEECCHHHHHHCEEEEEECCEEEEE
MDKDHMQLNTDQVLDYVDEYTIGVVGILGITYTGRYDDIYALNEKLEEYNSKTDYKVYIH
CCCCCCEECHHHHHHHHHHHHHHHHEEHHEEECCCCCCHHHHHHHHHHHCCCCCEEEEEE
VDAASGGFFTPFVEPDIIWDFRLKNVISINTSGHKYGLVYPGIGWVLWKDESYLPEELIF
EEECCCCEECCCCCCCEEEEEEECEEEEECCCCCEEEEEECCCEEEEECCCCCCCHHHHH
KVSYLGGEMPTMQINFSRSASHIIGQYYNFLRYGFEGYRTIHQKTSDVAQYLAHAVEQTG
HHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
YFDIYNDGSHLPIVCYKLKDDANVKWTLYDLADRLQMRGWQVPAYPLPKNLENIIIQRYV
CEEEECCCCCCCEEEEEECCCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
CRADLGFNMAEEFIQDFQASIQELNNAHILFHDTQQSGVHGFTH
HHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]