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The map label for this gene is flbD [H]

Identifier: 226224780

GI number: 226224780

Start: 2256089

End: 2257456

Strand: Reverse

Name: flbD [H]

Synonym: Lm4b_02198

Alternate gene names: 226224780

Gene position: 2257456-2256089 (Counterclockwise)

Preceding gene: 226224781

Following gene: 226224779

Centisome position: 77.5

GC content: 38.74

Gene sequence:

>1368_bases
ATGTTTGACTTAATGGATAACTTGCTGGGAGTACAGTCATTTGGTGAATTAAATAAGGACATGCCAACGACACTTTTTGT
CACGGATGCAGATGGTAATATTTTAATTTCTAATAAATTCACTGCGCTCACAGTTGGAATGTCGCTCGAAGAATTGCTTC
GTTGTAATGTAAGAGACCTAGTGGAAGACGGTGTATACAATGATTCTGTCACACTAGAAGCAATCCGCACAAAACAAAAG
AAAACAAAAGTGATCAATACGAAGAAAGGTTTTAGCATCCGCTCGACTTCTACGCCAATACTTTATCCTGATGGCACGGT
GCATTTAGTTGTTACTATGTCAGATGAAACGCAACCAGATAGTTTTAAAACATGGCGTGGTGAGGCAATTTCGGGGAATA
AAGAGTCGCTTCTTCTTGAGGATTATAAAGAAAATGATGGCACTGTGATAGTAGCAGAGAGCGTCGCGATGAAGCAGATA
GTTCGTGTGTGCAACCAGATCGCTCCGTTTGACAGTAAAGTCTTATTATACGGGGAGTCCGGTACTGGTAAAGAAGTCTT
GTCGCGCTATATTCATGAAAAAAGTGAACAAGCTGCTGGACCTTTTATCTCGATTAACTGTGCGGCAATCCCAAAAGCAT
TATTTGAATCTGAGCTTTTTGGACATGAAAAAGGTTCATTTACCGGTGCGGATATTGAAAAACCCGGGATGCTTGAACTA
GCTGATGGTGGCACATTATTTTTAGATGAAATTTCTGAAATGCCACTAGAGCTACAAGCGAAAATGCTCCGAGTGCTTGA
AACTGGAGAGGTAAGAAGGCTTGGTTCAACAACCGAAACGAAACGCCGCTTTAGACTGATTTCAGCGACCAATCGAAACC
TTGGGGAAATGGTTGAAAAAGGAACATTTAGGCGTGATTTATATTATCGTATTAATGTTGTGCCGGTTCACATTCCAGCA
CTCAGAGAGCGACCGCAAGATATTATTGGTCTTGCACGTCAGTTCATACAAAAATTCAATCAAAAATACCAAAAAGATTT
TCAGTTAAGTGGCGATAAAACGAAAGAATTACTTTCTCATAATTGGCCAGGAAATGTTCGTGAACTTAGAAACCAGATTG
AGCGATTAGTTGTTATGTCTGGAAATAAAGAAGTGACTGTTGCAGAAATAGATGATTTCGCACTAGACTTACATTTTAAA
GAACAAACAAAAAAAGACTCACTTTATTTGAAAGATTATTTGCAAGACGTGGAAAAACATTTTATATTGCGAGTGCTCGA
AGAAAGTAATGGAAACGTGACAAAAGCTGCTAGTACACTTGGAATTCATCGTTCTGTTTTATATCGAAAACTAAAAACAC
TTCATTAA

Upstream 100 bases:

>100_bases
CGCAAAAAAACGAGTACTTCTAGGAGATAGCTTATGTTATAATATATTGTGTAATGATGAGCAAATAACTAGGAAACTAG
TCCAAAAGGATGAGCTTTTA

Downstream 100 bases:

>100_bases
ATAGAAGGCCTAATCGGATTTTGCGATTAGGCCTTTTTTGTTGCGTTTTCGCGACTTCTGTTTGTAAAAACCATCATTTT
GACGTCTCTTTTTTGCGACA

Product: sigma-54-dependent transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MFDLMDNLLGVQSFGELNKDMPTTLFVTDADGNILISNKFTALTVGMSLEELLRCNVRDLVEDGVYNDSVTLEAIRTKQK
KTKVINTKKGFSIRSTSTPILYPDGTVHLVVTMSDETQPDSFKTWRGEAISGNKESLLLEDYKENDGTVIVAESVAMKQI
VRVCNQIAPFDSKVLLYGESGTGKEVLSRYIHEKSEQAAGPFISINCAAIPKALFESELFGHEKGSFTGADIEKPGMLEL
ADGGTLFLDEISEMPLELQAKMLRVLETGEVRRLGSTTETKRRFRLISATNRNLGEMVEKGTFRRDLYYRINVVPVHIPA
LRERPQDIIGLARQFIQKFNQKYQKDFQLSGDKTKELLSHNWPGNVRELRNQIERLVVMSGNKEVTVAEIDDFALDLHFK
EQTKKDSLYLKDYLQDVEKHFILRVLEESNGNVTKAASTLGIHRSVLYRKLKTLH

Sequences:

>Translated_455_residues
MFDLMDNLLGVQSFGELNKDMPTTLFVTDADGNILISNKFTALTVGMSLEELLRCNVRDLVEDGVYNDSVTLEAIRTKQK
KTKVINTKKGFSIRSTSTPILYPDGTVHLVVTMSDETQPDSFKTWRGEAISGNKESLLLEDYKENDGTVIVAESVAMKQI
VRVCNQIAPFDSKVLLYGESGTGKEVLSRYIHEKSEQAAGPFISINCAAIPKALFESELFGHEKGSFTGADIEKPGMLEL
ADGGTLFLDEISEMPLELQAKMLRVLETGEVRRLGSTTETKRRFRLISATNRNLGEMVEKGTFRRDLYYRINVVPVHIPA
LRERPQDIIGLARQFIQKFNQKYQKDFQLSGDKTKELLSHNWPGNVRELRNQIERLVVMSGNKEVTVAEIDDFALDLHFK
EQTKKDSLYLKDYLQDVEKHFILRVLEESNGNVTKAASTLGIHRSVLYRKLKTLH
>Mature_455_residues
MFDLMDNLLGVQSFGELNKDMPTTLFVTDADGNILISNKFTALTVGMSLEELLRCNVRDLVEDGVYNDSVTLEAIRTKQK
KTKVINTKKGFSIRSTSTPILYPDGTVHLVVTMSDETQPDSFKTWRGEAISGNKESLLLEDYKENDGTVIVAESVAMKQI
VRVCNQIAPFDSKVLLYGESGTGKEVLSRYIHEKSEQAAGPFISINCAAIPKALFESELFGHEKGSFTGADIEKPGMLEL
ADGGTLFLDEISEMPLELQAKMLRVLETGEVRRLGSTTETKRRFRLISATNRNLGEMVEKGTFRRDLYYRINVVPVHIPA
LRERPQDIIGLARQFIQKFNQKYQKDFQLSGDKTKELLSHNWPGNVRELRNQIERLVVMSGNKEVTVAEIDDFALDLHFK
EQTKKDSLYLKDYLQDVEKHFILRVLEESNGNVTKAASTLGIHRSVLYRKLKTLH

Specific function: Activation of sigma-54-dependent flagellar gene promoters and strong negative autoregulatory effects on its own promoter. The synthesis and function of flbD in C.crescentus is controlled by an internal cell-cycle clock [H]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=308, Percent_Identity=45.7792207792208, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI87082117, Length=360, Percent_Identity=40.2777777777778, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI1790437, Length=303, Percent_Identity=42.2442244224422, Blast_Score=240, Evalue=2e-64,
Organism=Escherichia coli, GI1789087, Length=310, Percent_Identity=43.5483870967742, Blast_Score=239, Evalue=3e-64,
Organism=Escherichia coli, GI1790299, Length=349, Percent_Identity=39.2550143266476, Blast_Score=238, Evalue=7e-64,
Organism=Escherichia coli, GI1789233, Length=319, Percent_Identity=42.3197492163009, Blast_Score=231, Evalue=5e-62,
Organism=Escherichia coli, GI1788905, Length=308, Percent_Identity=39.9350649350649, Blast_Score=227, Evalue=9e-61,
Organism=Escherichia coli, GI87081872, Length=319, Percent_Identity=38.5579937304075, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1786524, Length=324, Percent_Identity=37.3456790123457, Blast_Score=205, Evalue=6e-54,
Organism=Escherichia coli, GI87082152, Length=234, Percent_Identity=44.4444444444444, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI1787583, Length=307, Percent_Identity=36.4820846905537, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI87081858, Length=295, Percent_Identity=31.5254237288136, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI1789828, Length=252, Percent_Identity=32.9365079365079, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 51419; Mature: 51419

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDLMDNLLGVQSFGELNKDMPTTLFVTDADGNILISNKFTALTVGMSLEELLRCNVRDL
CHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEECCCEEEEECCCHHHHHCCCHHHH
VEDGVYNDSVTLEAIRTKQKKTKVINTKKGFSIRSTSTPILYPDGTVHLVVTMSDETQPD
HHCCCCCCCEEHHHHHHHHHHHHEEECCCCCEEECCCCCEEECCCEEEEEEEECCCCCCC
SFKTWRGEAISGNKESLLLEDYKENDGTVIVAESVAMKQIVRVCNQIAPFDSKVLLYGES
CCCCCCCCCCCCCCCCEEEEECCCCCCEEEEECHHHHHHHHHHHHHHCCCCCEEEEEECC
GTGKEVLSRYIHEKSEQAAGPFISINCAAIPKALFESELFGHEKGSFTGADIEKPGMLEL
CCHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEE
ADGGTLFLDEISEMPLELQAKMLRVLETGEVRRLGSTTETKRRFRLISATNRNLGEMVEK
CCCCEEEEHHHHCCCHHHHHHHHHHHHCCCHHHCCCCHHHHHHEEEEECCCCCHHHHHHC
GTFRRDLYYRINVVPVHIPALRERPQDIIGLARQFIQKFNQKYQKDFQLSGDKTKELLSH
CCCCEEEEEEEEEEEEECCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHC
NWPGNVRELRNQIERLVVMSGNKEVTVAEIDDFALDLHFKEQTKKDSLYLKDYLQDVEKH
CCCCCHHHHHHHHHEEEEECCCCEEEEEEECCEEEEEEEHHCCCCCHHHHHHHHHHHHHH
FILRVLEESNGNVTKAASTLGIHRSVLYRKLKTLH
HHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFDLMDNLLGVQSFGELNKDMPTTLFVTDADGNILISNKFTALTVGMSLEELLRCNVRDL
CHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEECCCEEEEECCCHHHHHCCCHHHH
VEDGVYNDSVTLEAIRTKQKKTKVINTKKGFSIRSTSTPILYPDGTVHLVVTMSDETQPD
HHCCCCCCCEEHHHHHHHHHHHHEEECCCCCEEECCCCCEEECCCEEEEEEEECCCCCCC
SFKTWRGEAISGNKESLLLEDYKENDGTVIVAESVAMKQIVRVCNQIAPFDSKVLLYGES
CCCCCCCCCCCCCCCCEEEEECCCCCCEEEEECHHHHHHHHHHHHHHCCCCCEEEEEECC
GTGKEVLSRYIHEKSEQAAGPFISINCAAIPKALFESELFGHEKGSFTGADIEKPGMLEL
CCHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEE
ADGGTLFLDEISEMPLELQAKMLRVLETGEVRRLGSTTETKRRFRLISATNRNLGEMVEK
CCCCEEEEHHHHCCCHHHHHHHHHHHHCCCHHHCCCCHHHHHHEEEEECCCCCHHHHHHC
GTFRRDLYYRINVVPVHIPALRERPQDIIGLARQFIQKFNQKYQKDFQLSGDKTKELLSH
CCCCEEEEEEEEEEEEECCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHC
NWPGNVRELRNQIERLVVMSGNKEVTVAEIDDFALDLHFKEQTKKDSLYLKDYLQDVEKH
CCCCCHHHHHHHHHEEEEECCCCEEEEEEECCEEEEEEEHHCCCCCHHHHHHHHHHHHHH
FILRVLEESNGNVTKAASTLGIHRSVLYRKLKTLH
HHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2315326; 11259647 [H]