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The map label for this gene is eutD

Identifier: 226224708

GI number: 226224708

Start: 2182457

End: 2183434

Strand: Reverse

Name: eutD

Synonym: Lm4b_02124

Alternate gene names: 226224708

Gene position: 2183434-2182457 (Counterclockwise)

Preceding gene: 226224712

Following gene: 226224705

Centisome position: 74.96

GC content: 41.0

Gene sequence:

>978_bases
ATGAGTGATTTATTTACTACAATCAAAGGACAAGTTACTGGGAAAAACGTGCGCATCGTACTACCTGAAGGAACAGATGA
ACGTATTGTTGGAGCAGCTGCACGTCTGCAAAAAGAAAATATCGTAAAACCAATTTTGCTTGGTAACAAAGCTGAAATTG
AAGCAAAAGCAAAAGAAATTGGCGTTTCTGTTGAAGGAATCGCTATTCACGAACCTGCGACCGATCCACTTTTTGATGAA
TTAGTGGCTGCATTTGTAGAACGTCGTAAAGGGAAAGCAACAGAAGAAGCTGCACGTAAAATGCTCGTTGATCCAAACTA
TTTTGGAACAATGCTTGTATACACTGGAAAAGCAGAAGGTCTTGTAAGTGGCGCGGCTCACTCTACTGGTGACACTGTTC
GCCCAGCACTACAAATTATTAAAACAAAACCAGGCGTAAGTAAAGTTGCCGGCGCAATGATTATGGTTCGCGGTGAAGAA
CGCTATTTATTCAGTGATGTAGCTATCAACATTGCACCAGTTGCAGCAGACTTAGCGGAAAATGCGATTGTAAGTGCGGA
AACAGCAGAAATCTTCGGCATTGACCCGCGTGTTGCAATGTTAAGTTTCTCTACAAAAGGTTCTGCAAAATCCGATGAAA
CGGAAAAAGTCGTAGAAGCAACTGCTCTTGCAAAAGAAAAAGCACCTGAATTAACGCTTGATGGTGAATTCCAATTTGAC
GCAGCATTTGTTCCAACGGTTGCTGAGAAAAAAGCGCCAGGATCCGTTATTAAAGGGGACGCAAATGTATTTATCTTCCC
AAGCCTAGAAGCAGGAAACATTGGTTACAAAATTGCGCAACGTTTAGGTAACTTTGAAGCAGTTGGCCCAATTTTACAAG
GCTTAAATGCACCAGTTAATGATTTATCTCGTGGTTGTAATACAGATGACGTTTATAACTTAACGTTAATTACAGCAGCG
CAAGCAGTGAATAAATAA

Upstream 100 bases:

>100_bases
AGTGCTGGAGCTGTTTTTAATCACTTTTTTTCTGTCAAGTTGTTCTTTTTTATAGTAAAATGGTAACTATAATATGTATA
TTAATGGAGGCAATTAACAC

Downstream 100 bases:

>100_bases
TATGCAAAAGCAGTAGACTAAATTGTCTACTGCTTTTTTTATACCATTTTTTCGAGGAAGTAATTCATCCAGCGATTGTC
GTTAGTAAGTTCAGGATGGA

Product: phosphotransacetylase

Products: NA

Alternate protein names: Phosphotransacetylase; Vegetative protein 43; VEG43 [H]

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MSDLFTTIKGQVTGKNVRIVLPEGTDERIVGAAARLQKENIVKPILLGNKAEIEAKAKEIGVSVEGIAIHEPATDPLFDE
LVAAFVERRKGKATEEAARKMLVDPNYFGTMLVYTGKAEGLVSGAAHSTGDTVRPALQIIKTKPGVSKVAGAMIMVRGEE
RYLFSDVAINIAPVAADLAENAIVSAETAEIFGIDPRVAMLSFSTKGSAKSDETEKVVEATALAKEKAPELTLDGEFQFD
AAFVPTVAEKKAPGSVIKGDANVFIFPSLEAGNIGYKIAQRLGNFEAVGPILQGLNAPVNDLSRGCNTDDVYNLTLITAA
QAVNK

Sequences:

>Translated_325_residues
MSDLFTTIKGQVTGKNVRIVLPEGTDERIVGAAARLQKENIVKPILLGNKAEIEAKAKEIGVSVEGIAIHEPATDPLFDE
LVAAFVERRKGKATEEAARKMLVDPNYFGTMLVYTGKAEGLVSGAAHSTGDTVRPALQIIKTKPGVSKVAGAMIMVRGEE
RYLFSDVAINIAPVAADLAENAIVSAETAEIFGIDPRVAMLSFSTKGSAKSDETEKVVEATALAKEKAPELTLDGEFQFD
AAFVPTVAEKKAPGSVIKGDANVFIFPSLEAGNIGYKIAQRLGNFEAVGPILQGLNAPVNDLSRGCNTDDVYNLTLITAA
QAVNK
>Mature_324_residues
SDLFTTIKGQVTGKNVRIVLPEGTDERIVGAAARLQKENIVKPILLGNKAEIEAKAKEIGVSVEGIAIHEPATDPLFDEL
VAAFVERRKGKATEEAARKMLVDPNYFGTMLVYTGKAEGLVSGAAHSTGDTVRPALQIIKTKPGVSKVAGAMIMVRGEER
YLFSDVAINIAPVAADLAENAIVSAETAEIFGIDPRVAMLSFSTKGSAKSDETEKVVEATALAKEKAPELTLDGEFQFDA
AFVPTVAEKKAPGSVIKGDANVFIFPSLEAGNIGYKIAQRLGNFEAVGPILQGLNAPVNDLSRGCNTDDVYNLTLITAAQ
AVNK

Specific function: Conversion of acetate to acetyl-CoA; second step. [C]

COG id: COG0280

COG function: function code C; Phosphotransacetylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788635, Length=310, Percent_Identity=47.4193548387097, Blast_Score=258, Evalue=3e-70,
Organism=Escherichia coli, GI1788800, Length=308, Percent_Identity=43.1818181818182, Blast_Score=248, Evalue=3e-67,
Organism=Escherichia coli, GI1788806, Length=313, Percent_Identity=31.629392971246, Blast_Score=163, Evalue=2e-41,

Paralogues:

None

Copy number: 1420 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1250 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012147
- InterPro:   IPR004614
- InterPro:   IPR002505 [H]

Pfam domain/function: PF01515 PTA_PTB [H]

EC number: =2.3.1.8 [H]

Molecular weight: Translated: 34414; Mature: 34283

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLFTTIKGQVTGKNVRIVLPEGTDERIVGAAARLQKENIVKPILLGNKAEIEAKAKEI
CCCHHEEHCCEEECCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHH
GVSVEGIAIHEPATDPLFDELVAAFVERRKGKATEEAARKMLVDPNYFGTMLVYTGKAEG
CCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEEEECCCCC
LVSGAAHSTGDTVRPALQIIKTKPGVSKVAGAMIMVRGEERYLFSDVAINIAPVAADLAE
EEECCCCCCCCHHHHHHHHHHCCCCHHHHCCEEEEEECCCEEEEEEEEEEEEHHHHHHHH
NAIVSAETAEIFGIDPRVAMLSFSTKGSAKSDETEKVVEATALAKEKAPELTLDGEFQFD
CCEEECCCCEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCEEEC
AAFVPTVAEKKAPGSVIKGDANVFIFPSLEAGNIGYKIAQRLGNFEAVGPILQGLNAPVN
CEECCCHHCCCCCCCEEECCCCEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCHH
DLSRGCNTDDVYNLTLITAAQAVNK
HHHCCCCCCCEEEEEEEEHHHHHCC
>Mature Secondary Structure 
SDLFTTIKGQVTGKNVRIVLPEGTDERIVGAAARLQKENIVKPILLGNKAEIEAKAKEI
CCHHEEHCCEEECCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHH
GVSVEGIAIHEPATDPLFDELVAAFVERRKGKATEEAARKMLVDPNYFGTMLVYTGKAEG
CCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEEEECCCCC
LVSGAAHSTGDTVRPALQIIKTKPGVSKVAGAMIMVRGEERYLFSDVAINIAPVAADLAE
EEECCCCCCCCHHHHHHHHHHCCCCHHHHCCEEEEEECCCEEEEEEEEEEEEHHHHHHHH
NAIVSAETAEIFGIDPRVAMLSFSTKGSAKSDETEKVVEATALAKEKAPELTLDGEFQFD
CCEEECCCCEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCEEEC
AAFVPTVAEKKAPGSVIKGDANVFIFPSLEAGNIGYKIAQRLGNFEAVGPILQGLNAPVN
CEECCCHHCCCCCCCEEECCCCEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCHH
DLSRGCNTDDVYNLTLITAAQAVNK
HHHCCCCCCCEEEEEEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 9298659 [H]