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The map label for this gene is murE

Identifier: 226224644

GI number: 226224644

Start: 2119467

End: 2120942

Strand: Reverse

Name: murE

Synonym: Lm4b_02059

Alternate gene names: 226224644

Gene position: 2120942-2119467 (Counterclockwise)

Preceding gene: 226224645

Following gene: 226224643

Centisome position: 72.82

GC content: 40.31

Gene sequence:

>1476_bases
ATGAAGTTAAATGAACTAATGCAAGCTATCCCGGTTTTTACTGGCGAGGCAAGTGAAACAATTGAAATTAGCCATATCGC
CCAAGATAGTAGAAAAGTAAAACCAGGGACGTTGTTTATTTGTATAGATGGAGAATTGGTGGATGGACATCAATTTGCAT
CACGTGCAGTAGAACTAGGAGCGGTAGCAATTATCGCGGAAAAACAATTAGATGTATCGATTCCTGTCATTTATGTAAGA
GATTCAAAACGTGCTATGGCGATGTTAGCCGATTACTTTTATGGATCTCCAACCCAAGCATTAAAGCTCGTTGGGATTAC
TGGGACAAATGGGAAGACGACCGTGAGCCACTTAGTGGAACAAATTGTGCGCGAAAATGGCGAGCAAACAGGTTTGATTG
GCACGATGTATCGCAAAATTGGCGACCAAATTTTAGAAACAAAAAATACAACGCCAGATAGCCTCACTTTACAAGAAACA
TTCCGGGACATGTTACTTAGTGGAGTGAGCACAGCTGTGATGGAAGTATCCTCGCATGCACTCGTTCAAGGTCGCGTATA
TGGTTCAGATTACGATGTTGCTGTATTTATGAATTTGTCTCAAGATCATTTGGATTATCATCATACAATGGAAGAATACG
CCAATGCAAAAAGCTTATTGTTTGCGCAACTAGGCAACAGCTACCATACAAGTAACCCAAAAATTGCTGTGTTAAACGCG
GATGACGAGGAAAGCGTGCGTATGCAAAAAGCAACAGCTGCTCATGTAATCACTTTTGGGATAAAACAACAAGCTGACTT
CAAAGCAAGTAATATCAGAATTACTAGCCATGGTTCTACATTTGATTTACGGACACCAATTGGTAATTTTACATTGAAAA
TAAAAATGATTGGAAACTTTAGTGTTTATAACGTATTGGCTGCGATTGCAACTAGCTTTGCGCTACATATCCCTATGGAA
AAAGCAATTAAAACAGTGGAAAGCATACCGGGTGTCAAAGGCCGTTTTGAACTTGTGCATGCAGGGCAAGAGTTCCCTGT
TATAGTTGATTACGCCCATACTCCAGACGGCTTGCTAAATGTATTAGAAACAATTGCTGAGTTTGCAGAAAAACGCGTTT
TTGTCGTTGTTGGTTGTGGCGGGGACCGTGATAAAGGAAAACGTCCACAAATGGCAAAAATCGCTGTAGATTATGCAACA
AATCCTATTTTTACATCAGATAATCCGCGGAGTGAAAATCCGCGTGCGATTATTGAAGATATGATTCAAGGTGTTCCATA
TAGTGATTCTTATGTGGTTCACGAAAACCGCCGAGATGCGATTCGTTTTGCAGTAAATGAAGCAGAAGCAGGGGATGTTA
TTCTGATTGCTGGAAAAGGTCATGAAGATTATCAAGTGATTGGTGACGAGGTTATTGATTTTGATGACCGAGTAGAAGCC
CGAATAGCTATTGAAAAAAAACTTGGACTCGCATAA

Upstream 100 bases:

>100_bases
TCGTGGTAGATATAGTTTGTTGTTTTTGCTAAGATAGAAAAGGATTCTTTTTGGTTTTAGCAAAAATAACAGAAAGAATA
AGTTTGAGGAGATGGCAAGA

Downstream 100 bases:

>100_bases
AATATGAAGTGAAGTGCTTTGACAAACAGATTGGGGAGAATTTATTGTGTCTTTATACATGTTAGTATCAACATTTGCAG
TTGCTTTTATCATTACGGTG

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MKLNELMQAIPVFTGEASETIEISHIAQDSRKVKPGTLFICIDGELVDGHQFASRAVELGAVAIIAEKQLDVSIPVIYVR
DSKRAMAMLADYFYGSPTQALKLVGITGTNGKTTVSHLVEQIVRENGEQTGLIGTMYRKIGDQILETKNTTPDSLTLQET
FRDMLLSGVSTAVMEVSSHALVQGRVYGSDYDVAVFMNLSQDHLDYHHTMEEYANAKSLLFAQLGNSYHTSNPKIAVLNA
DDEESVRMQKATAAHVITFGIKQQADFKASNIRITSHGSTFDLRTPIGNFTLKIKMIGNFSVYNVLAAIATSFALHIPME
KAIKTVESIPGVKGRFELVHAGQEFPVIVDYAHTPDGLLNVLETIAEFAEKRVFVVVGCGGDRDKGKRPQMAKIAVDYAT
NPIFTSDNPRSENPRAIIEDMIQGVPYSDSYVVHENRRDAIRFAVNEAEAGDVILIAGKGHEDYQVIGDEVIDFDDRVEA
RIAIEKKLGLA

Sequences:

>Translated_491_residues
MKLNELMQAIPVFTGEASETIEISHIAQDSRKVKPGTLFICIDGELVDGHQFASRAVELGAVAIIAEKQLDVSIPVIYVR
DSKRAMAMLADYFYGSPTQALKLVGITGTNGKTTVSHLVEQIVRENGEQTGLIGTMYRKIGDQILETKNTTPDSLTLQET
FRDMLLSGVSTAVMEVSSHALVQGRVYGSDYDVAVFMNLSQDHLDYHHTMEEYANAKSLLFAQLGNSYHTSNPKIAVLNA
DDEESVRMQKATAAHVITFGIKQQADFKASNIRITSHGSTFDLRTPIGNFTLKIKMIGNFSVYNVLAAIATSFALHIPME
KAIKTVESIPGVKGRFELVHAGQEFPVIVDYAHTPDGLLNVLETIAEFAEKRVFVVVGCGGDRDKGKRPQMAKIAVDYAT
NPIFTSDNPRSENPRAIIEDMIQGVPYSDSYVVHENRRDAIRFAVNEAEAGDVILIAGKGHEDYQVIGDEVIDFDDRVEA
RIAIEKKLGLA
>Mature_491_residues
MKLNELMQAIPVFTGEASETIEISHIAQDSRKVKPGTLFICIDGELVDGHQFASRAVELGAVAIIAEKQLDVSIPVIYVR
DSKRAMAMLADYFYGSPTQALKLVGITGTNGKTTVSHLVEQIVRENGEQTGLIGTMYRKIGDQILETKNTTPDSLTLQET
FRDMLLSGVSTAVMEVSSHALVQGRVYGSDYDVAVFMNLSQDHLDYHHTMEEYANAKSLLFAQLGNSYHTSNPKIAVLNA
DDEESVRMQKATAAHVITFGIKQQADFKASNIRITSHGSTFDLRTPIGNFTLKIKMIGNFSVYNVLAAIATSFALHIPME
KAIKTVESIPGVKGRFELVHAGQEFPVIVDYAHTPDGLLNVLETIAEFAEKRVFVVVGCGGDRDKGKRPQMAKIAVDYAT
NPIFTSDNPRSENPRAIIEDMIQGVPYSDSYVVHENRRDAIRFAVNEAEAGDVILIAGKGHEDYQVIGDEVIDFDDRVEA
RIAIEKKLGLA

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=466, Percent_Identity=37.5536480686695, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1786274, Length=378, Percent_Identity=26.4550264550265, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 53953; Mature: 53953

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNELMQAIPVFTGEASETIEISHIAQDSRKVKPGTLFICIDGELVDGHQFASRAVELG
CCHHHHHHHHCCCCCCCCCEEEEEEHHCCCCCCCCCEEEEEECCEEECCHHHHHHHHHHC
AVAIIAEKQLDVSIPVIYVRDSKRAMAMLADYFYGSPTQALKLVGITGTNGKTTVSHLVE
EEEEEEECCCCCEEEEEEEECCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHH
QIVRENGEQTGLIGTMYRKIGDQILETKNTTPDSLTLQETFRDMLLSGVSTAVMEVSSHA
HHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCE
LVQGRVYGSDYDVAVFMNLSQDHLDYHHTMEEYANAKSLLFAQLGNSYHTSNPKIAVLNA
EEEEEEECCCCCEEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHCCCEECCCCEEEEEEC
DDEESVRMQKATAAHVITFGIKQQADFKASNIRITSHGSTFDLRTPIGNFTLKIKMIGNF
CCHHHHHHHHHHHEEEEEEECCCCCCCCCCCEEEEECCCEEEEECCCCCEEEEEEEECCC
SVYNVLAAIATSFALHIPMEKAIKTVESIPGVKGRFELVHAGQEFPVIVDYAHTPDGLLN
HHHHHHHHHHHHHEEECCHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEECCCCHHHHH
VLETIAEFAEKRVFVVVGCGGDRDKGKRPQMAKIAVDYATNPIFTSDNPRSENPRAIIED
HHHHHHHHHCCEEEEEEECCCCCCCCCCCCEEEEEEEECCCCEECCCCCCCCCCHHHHHH
MIQGVPYSDSYVVHENRRDAIRFAVNEAEAGDVILIAGKGHEDYQVIGDEVIDFDDRVEA
HHCCCCCCCCEEEECCCCCEEEEEECCCCCCCEEEEECCCCCCHHHHCHHHCCCCCCCEE
RIAIEKKLGLA
EEEEEHHCCCC
>Mature Secondary Structure
MKLNELMQAIPVFTGEASETIEISHIAQDSRKVKPGTLFICIDGELVDGHQFASRAVELG
CCHHHHHHHHCCCCCCCCCEEEEEEHHCCCCCCCCCEEEEEECCEEECCHHHHHHHHHHC
AVAIIAEKQLDVSIPVIYVRDSKRAMAMLADYFYGSPTQALKLVGITGTNGKTTVSHLVE
EEEEEEECCCCCEEEEEEEECCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHH
QIVRENGEQTGLIGTMYRKIGDQILETKNTTPDSLTLQETFRDMLLSGVSTAVMEVSSHA
HHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCE
LVQGRVYGSDYDVAVFMNLSQDHLDYHHTMEEYANAKSLLFAQLGNSYHTSNPKIAVLNA
EEEEEEECCCCCEEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHCCCEECCCCEEEEEEC
DDEESVRMQKATAAHVITFGIKQQADFKASNIRITSHGSTFDLRTPIGNFTLKIKMIGNF
CCHHHHHHHHHHHEEEEEEECCCCCCCCCCCEEEEECCCEEEEECCCCCEEEEEEEECCC
SVYNVLAAIATSFALHIPMEKAIKTVESIPGVKGRFELVHAGQEFPVIVDYAHTPDGLLN
HHHHHHHHHHHHHEEECCHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEECCCCHHHHH
VLETIAEFAEKRVFVVVGCGGDRDKGKRPQMAKIAVDYATNPIFTSDNPRSENPRAIIED
HHHHHHHHHCCEEEEEEECCCCCCCCCCCCEEEEEEEECCCCEECCCCCCCCCCHHHHHH
MIQGVPYSDSYVVHENRRDAIRFAVNEAEAGDVILIAGKGHEDYQVIGDEVIDFDDRVEA
HHCCCCCCCCEEEECCCCCEEEEEECCCCCCCEEEEECCCCCCHHHHCHHHCCCCCCCEE
RIAIEKKLGLA
EEEEEHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]