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The map label for this gene is pbpB

Identifier: 226224645

GI number: 226224645

Start: 2121117

End: 2123372

Strand: Reverse

Name: pbpB

Synonym: Lm4b_02060

Alternate gene names: 226224645

Gene position: 2123372-2121117 (Counterclockwise)

Preceding gene: 226224646

Following gene: 226224644

Centisome position: 72.9

GC content: 38.08

Gene sequence:

>2256_bases
ATGAAACGGCGTATAGGTAACATGAGAAGGGGAGCTCTTGTGCTTTTTATCTTTTTCACAATTCTCTTTCTCTTGGTTTC
TGGCCGTTTTCTTTATTTGCAAATTACGGGAGAAGCAAATGGGGTACCTCTTGCAGCAAAAGCATCTAAACAACATTTGA
AAAGCAGTGTTCTCGAAGCAAAGCGTGGCTCCATTTTAGATCGTAAAGGAGAAGTGCTAGCGGAGGATACGGCTTCTTAC
ACGGTTGCAGCAGTAGTTAGCGATAAACTTTCCAAAACAACGAAAAATCCAATGCGTGTTGTTGACGAAGAAAAAACGGC
TCGAATTCTTGCCAAGTATATTCCTATGGATGAATCTGATATTCTAGATAAACTCAATGAAAAAGGAAAATACCAAGTCG
AATTCGGCGCTGCCGGTAAAAATATTTCTACAGAAACAAAAGCAAAAATTGACAAAGAAAATTTACCAGGTATTGAATTT
ACTCGTCAATCAGAACGTTTCTATCCTAATGGAACGTTTGCAACACAATTAATTGGTTTCGCCCAACAAGAAGAAGAAAA
GAATACTACAGTTTTAGAAGGTAAAATGGGTATTGAATCCCGTTACAATGATATTTTGACAGGCAAGAATGGCAAAATTG
ATTACAGTACAGACAGAATGGGCTATATTTTGCCTAACTCTAAAAACAAAGTAACGGAAGCAAAAGACGGCAAAGATATT
ACGCTAACGCTAGATAAAAAAATTCAAACCTTTTTAGAAGATACGATGACAACTGTGGATGCAAAGTACAAACCTAAAAA
CATGATGGCAGTTGTAGCTGATCCGAAAACAGGTGAAATTTTGGCTATTAGTCAACGTCCTTCTTTCAACCCTGCTGATC
GATCTACTATTACTGGAAACAGCTCCAGTATTTGGCAAGATTTACCTGTTGAGTATGCTTATGAGCCAGGTTCGGTAATG
AAAATTATTTCTCTTGCATCTGCTATTGATACGAACGCATATAATGCCAATGAATATTATCAATCGGGTTCATACAAAGT
AGGAGATATCGCAATTCATGACCATAATAACGGTAATGGTTGGGGGTCTATTACGTACCGTGAAGGGGTAGAACGCTCTA
GTAACGTTGCATTTGCCAAACTTTTAAACAAAATGGGGACCGATACGTTTAAAACATATTTAGACAAATTTGGTTTTGGT
AAGAAAACAGGTATTGCTTTACCTAATGAAACAAGCGGGAAAATTTTATATAACTATCCAATTGAGAAAGTAACAACCGT
TTTCGGCCAAGGAACAACCGTTTCAATGATGCAAATGATTCAAGCTGCTTCGGCTATCGCAAGTGATGGCACGATGAAAG
AACCATATGTTGTTTCAAGTGTCAAAGATCCAAACACAGGCGAAGAAACCGATACTAAAACAAAAATTGCTGGAAAACCA
ATTAGTGCAGAAACAGCGAAGAAAACGCGTGACGAATTAAAAAACGTTATTAGCGGCGAACATGGTACTGGTAAATTATA
TGCGATTCCGGGATACGATGTAGCTGGTAAAACCGGTACATCACAAATTCCAGATCCGAAAACTGGTAAGTATATGACTG
GAGCAGATAATTATATTTTCTCTTTCTTAGGAATGGCACCAGCGGATAATCCGGAGTTAGTAATCTATGTGACAATGCAA
CAACCACAATTGTCCGGCAGTCAGACTGGCGGAGAAGCAGTCTCAGAAGTTTTTAATCCTGTAATGAAAAACAGTCTACA
ATATATGAACATCAAACCAGGCGATGCAGAAAAACTAGCTTCCGAAAAAGTACCAGTCTTAGCAGGAAAAACAATAGATG
AAGCTAAAAAAATAGTGAAAGATAAAGGATTAGAACCAATTATAGTCGGCAACGGCAAAAAAATTGTCCAACAACTTCCA
CAAGCGAACGCCGAAATCATGCAAGGTCAAAAAGTTATTTTAATGACAGATGGTGACATGACTGCCCCTGACATGGTAAC
TTGGTCGAAAGACGATGCGCTAAAAGTTTCTGAAATAACTGGTATTCCGTTTGAGTTTAGTGGAAATGGTTACGTGAAGA
GCCAAAGTGTATCTGCGGGTAGCGTAATTAATAGTGAAACGAAAATGAAAATAACTCTCGCCCCACCAGAAGAAATAAGT
GCATTTAATCAAAATCACGATCAAGATACAGCAGAAAAAAATAAAAAAGCAACTGACATCCAAGAGAATGCCGGTATCGG
TGATTTGCTCAATTAA

Upstream 100 bases:

>100_bases
TAGAAGTAAAAGATTTAGGGAGATATGAACGTATTTTGAAAATCGCTAAAGAAAAAGGGCTAAAACTTGATGGCGATAAT
GTGAAAGTGGTAGATGGTCA

Downstream 100 bases:

>100_bases
TAATAGGAAGATAGAAGTATGTCAGCTTTACAAAGTGGCATACTTCTAACTTTAAATGCAATCTGTGTCTTTTGTCGTGG
TAGATATAGTTTGTTGTTTT

Product: penicillin-binding protein 2B

Products: NA

Alternate protein names: PBP-2B; Penicillin-binding protein B [H]

Number of amino acids: Translated: 751; Mature: 751

Protein sequence:

>751_residues
MKRRIGNMRRGALVLFIFFTILFLLVSGRFLYLQITGEANGVPLAAKASKQHLKSSVLEAKRGSILDRKGEVLAEDTASY
TVAAVVSDKLSKTTKNPMRVVDEEKTARILAKYIPMDESDILDKLNEKGKYQVEFGAAGKNISTETKAKIDKENLPGIEF
TRQSERFYPNGTFATQLIGFAQQEEEKNTTVLEGKMGIESRYNDILTGKNGKIDYSTDRMGYILPNSKNKVTEAKDGKDI
TLTLDKKIQTFLEDTMTTVDAKYKPKNMMAVVADPKTGEILAISQRPSFNPADRSTITGNSSSIWQDLPVEYAYEPGSVM
KIISLASAIDTNAYNANEYYQSGSYKVGDIAIHDHNNGNGWGSITYREGVERSSNVAFAKLLNKMGTDTFKTYLDKFGFG
KKTGIALPNETSGKILYNYPIEKVTTVFGQGTTVSMMQMIQAASAIASDGTMKEPYVVSSVKDPNTGEETDTKTKIAGKP
ISAETAKKTRDELKNVISGEHGTGKLYAIPGYDVAGKTGTSQIPDPKTGKYMTGADNYIFSFLGMAPADNPELVIYVTMQ
QPQLSGSQTGGEAVSEVFNPVMKNSLQYMNIKPGDAEKLASEKVPVLAGKTIDEAKKIVKDKGLEPIIVGNGKKIVQQLP
QANAEIMQGQKVILMTDGDMTAPDMVTWSKDDALKVSEITGIPFEFSGNGYVKSQSVSAGSVINSETKMKITLAPPEEIS
AFNQNHDQDTAEKNKKATDIQENAGIGDLLN

Sequences:

>Translated_751_residues
MKRRIGNMRRGALVLFIFFTILFLLVSGRFLYLQITGEANGVPLAAKASKQHLKSSVLEAKRGSILDRKGEVLAEDTASY
TVAAVVSDKLSKTTKNPMRVVDEEKTARILAKYIPMDESDILDKLNEKGKYQVEFGAAGKNISTETKAKIDKENLPGIEF
TRQSERFYPNGTFATQLIGFAQQEEEKNTTVLEGKMGIESRYNDILTGKNGKIDYSTDRMGYILPNSKNKVTEAKDGKDI
TLTLDKKIQTFLEDTMTTVDAKYKPKNMMAVVADPKTGEILAISQRPSFNPADRSTITGNSSSIWQDLPVEYAYEPGSVM
KIISLASAIDTNAYNANEYYQSGSYKVGDIAIHDHNNGNGWGSITYREGVERSSNVAFAKLLNKMGTDTFKTYLDKFGFG
KKTGIALPNETSGKILYNYPIEKVTTVFGQGTTVSMMQMIQAASAIASDGTMKEPYVVSSVKDPNTGEETDTKTKIAGKP
ISAETAKKTRDELKNVISGEHGTGKLYAIPGYDVAGKTGTSQIPDPKTGKYMTGADNYIFSFLGMAPADNPELVIYVTMQ
QPQLSGSQTGGEAVSEVFNPVMKNSLQYMNIKPGDAEKLASEKVPVLAGKTIDEAKKIVKDKGLEPIIVGNGKKIVQQLP
QANAEIMQGQKVILMTDGDMTAPDMVTWSKDDALKVSEITGIPFEFSGNGYVKSQSVSAGSVINSETKMKITLAPPEEIS
AFNQNHDQDTAEKNKKATDIQENAGIGDLLN
>Mature_751_residues
MKRRIGNMRRGALVLFIFFTILFLLVSGRFLYLQITGEANGVPLAAKASKQHLKSSVLEAKRGSILDRKGEVLAEDTASY
TVAAVVSDKLSKTTKNPMRVVDEEKTARILAKYIPMDESDILDKLNEKGKYQVEFGAAGKNISTETKAKIDKENLPGIEF
TRQSERFYPNGTFATQLIGFAQQEEEKNTTVLEGKMGIESRYNDILTGKNGKIDYSTDRMGYILPNSKNKVTEAKDGKDI
TLTLDKKIQTFLEDTMTTVDAKYKPKNMMAVVADPKTGEILAISQRPSFNPADRSTITGNSSSIWQDLPVEYAYEPGSVM
KIISLASAIDTNAYNANEYYQSGSYKVGDIAIHDHNNGNGWGSITYREGVERSSNVAFAKLLNKMGTDTFKTYLDKFGFG
KKTGIALPNETSGKILYNYPIEKVTTVFGQGTTVSMMQMIQAASAIASDGTMKEPYVVSSVKDPNTGEETDTKTKIAGKP
ISAETAKKTRDELKNVISGEHGTGKLYAIPGYDVAGKTGTSQIPDPKTGKYMTGADNYIFSFLGMAPADNPELVIYVTMQ
QPQLSGSQTGGEAVSEVFNPVMKNSLQYMNIKPGDAEKLASEKVPVLAGKTIDEAKKIVKDKGLEPIIVGNGKKIVQQLP
QANAEIMQGQKVILMTDGDMTAPDMVTWSKDDALKVSEITGIPFEFSGNGYVKSQSVSAGSVINSETKMKITLAPPEEIS
AFNQNHDQDTAEKNKKATDIQENAGIGDLLN

Specific function: Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis. PBP-2B is required for vegetative cell division and sporulation septation. Beta-lactamase inactivates the PBPs by acylating an essential serine residue in the active sit

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PASTA domains [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=594, Percent_Identity=27.2727272727273, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1786854, Length=556, Percent_Identity=24.2805755395683, Blast_Score=131, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005543
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03793 PASTA; PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 81908; Mature: 81908

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRIGNMRRGALVLFIFFTILFLLVSGRFLYLQITGEANGVPLAAKASKQHLKSSVLEA
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEHHHHHHHHHHHHHHH
KRGSILDRKGEVLAEDTASYTVAAVVSDKLSKTTKNPMRVVDEEKTARILAKYIPMDESD
HCCCCCCCCCCEEECCCCCEEEEEHHHHHHHHHCCCCHHHHCCHHHHHHHHHHCCCCHHH
ILDKLNEKGKYQVEFGAAGKNISTETKAKIDKENLPGIEFTRQSERFYPNGTFATQLIGF
HHHHHCCCCCEEEEECCCCCCCCCHHHHHCCCCCCCCCEEECCCCCCCCCCCHHHHHHHH
AQQEEEKNTTVLEGKMGIESRYNDILTGKNGKIDYSTDRMGYILPNSKNKVTEAKDGKDI
HHHHHCCCCEEEECCCCCHHHHHHHCCCCCCCEEECCCCEEEEECCCCCCCCCCCCCCEE
TLTLDKKIQTFLEDTMTTVDAKYKPKNMMAVVADPKTGEILAISQRPSFNPADRSTITGN
EEEEHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCEECCC
SSSIWQDLPVEYAYEPGSVMKIISLASAIDTNAYNANEYYQSGSYKVGDIAIHDHNNGNG
CCHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCCCCHHHHCCCCEEEEEEEEEECCCCCC
WGSITYREGVERSSNVAFAKLLNKMGTDTFKTYLDKFGFGKKTGIALPNETSGKILYNYP
EEEEEEHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECC
IEKVTTVFGQGTTVSMMQMIQAASAIASDGTMKEPYVVSSVKDPNTGEETDTKTKIAGKP
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCEECCCC
ISAETAKKTRDELKNVISGEHGTGKLYAIPGYDVAGKTGTSQIPDPKTGKYMTGADNYIF
CCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEECCCHHHHH
SFLGMAPADNPELVIYVTMQQPQLSGSQTGGEAVSEVFNPVMKNSLQYMNIKPGDAEKLA
HHHCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHH
SEKVPVLAGKTIDEAKKIVKDKGLEPIIVGNGKKIVQQLPQANAEIMQGQKVILMTDGDM
HCCCCEEECCCHHHHHHHHHHCCCCEEEECCCHHHHHHCCCCCHHHHCCCEEEEEECCCC
TAPDMVTWSKDDALKVSEITGIPFEFSGNGYVKSQSVSAGSVINSETKMKITLAPPEEIS
CCCCCEEECCCCCEEEEHCCCCCEEECCCCEEEECCCCCCCEECCCCEEEEEECCHHHHH
AFNQNHDQDTAEKNKKATDIQENAGIGDLLN
HHCCCCCHHHHHHCCCCCCHHHCCCCCCCCC
>Mature Secondary Structure
MKRRIGNMRRGALVLFIFFTILFLLVSGRFLYLQITGEANGVPLAAKASKQHLKSSVLEA
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEHHHHHHHHHHHHHHH
KRGSILDRKGEVLAEDTASYTVAAVVSDKLSKTTKNPMRVVDEEKTARILAKYIPMDESD
HCCCCCCCCCCEEECCCCCEEEEEHHHHHHHHHCCCCHHHHCCHHHHHHHHHHCCCCHHH
ILDKLNEKGKYQVEFGAAGKNISTETKAKIDKENLPGIEFTRQSERFYPNGTFATQLIGF
HHHHHCCCCCEEEEECCCCCCCCCHHHHHCCCCCCCCCEEECCCCCCCCCCCHHHHHHHH
AQQEEEKNTTVLEGKMGIESRYNDILTGKNGKIDYSTDRMGYILPNSKNKVTEAKDGKDI
HHHHHCCCCEEEECCCCCHHHHHHHCCCCCCCEEECCCCEEEEECCCCCCCCCCCCCCEE
TLTLDKKIQTFLEDTMTTVDAKYKPKNMMAVVADPKTGEILAISQRPSFNPADRSTITGN
EEEEHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCEECCC
SSSIWQDLPVEYAYEPGSVMKIISLASAIDTNAYNANEYYQSGSYKVGDIAIHDHNNGNG
CCHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCCCCHHHHCCCCEEEEEEEEEECCCCCC
WGSITYREGVERSSNVAFAKLLNKMGTDTFKTYLDKFGFGKKTGIALPNETSGKILYNYP
EEEEEEHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECC
IEKVTTVFGQGTTVSMMQMIQAASAIASDGTMKEPYVVSSVKDPNTGEETDTKTKIAGKP
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCEECCCC
ISAETAKKTRDELKNVISGEHGTGKLYAIPGYDVAGKTGTSQIPDPKTGKYMTGADNYIF
CCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEECCCHHHHH
SFLGMAPADNPELVIYVTMQQPQLSGSQTGGEAVSEVFNPVMKNSLQYMNIKPGDAEKLA
HHHCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHH
SEKVPVLAGKTIDEAKKIVKDKGLEPIIVGNGKKIVQQLPQANAEIMQGQKVILMTDGDM
HCCCCEEECCCHHHHHHHHHHCCCCEEEECCCHHHHHHCCCCCHHHHCCCEEEEEECCCC
TAPDMVTWSKDDALKVSEITGIPFEFSGNGYVKSQSVSAGSVINSETKMKITLAPPEEIS
CCCCCEEECCCCCEEEEHCCCCCEEECCCCEEEECCCCCCCEECCCCEEEEEECCHHHHH
AFNQNHDQDTAEKNKKATDIQENAGIGDLLN
HHCCCCCHHHHHHCCCCCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8244929; 9384377; 8289242 [H]