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The map label for this gene is frlB [H]

Identifier: 226224596

GI number: 226224596

Start: 2059929

End: 2060924

Strand: Reverse

Name: frlB [H]

Synonym: Lm4b_02010

Alternate gene names: 226224596

Gene position: 2060924-2059929 (Counterclockwise)

Preceding gene: 226224597

Following gene: 226224595

Centisome position: 70.76

GC content: 38.65

Gene sequence:

>996_bases
GTGCTAAAATTTGATGAACAAAAAGTGAGAGAAAACATGGAAGGAGCGCTAAAATTACGCCCGCAAATTAATGAAGTAGT
TGACCAAATACATGACCGCGGCTTTAGTAATATTTGCTGGCTAGGAATTGGCGGTACATACGCGTCCGCCATGCAAGCAG
TGGTGCATATGAAAGAAAAAACGGCATTAGAAACTTTTTATGAAAATGCTGCTGTGTTTCTAACGACAGGCAATAAACGT
GTTACTAAAGATACCCTTGTAGTCATTTCATCGGTAACTGGAAGTACGCAAGAAGTGGTAGATGCTGTGAAAAAATGTAA
TGAAATTGGCGCAACGGTATTTGGGTTTATTGATAAAGCAGAAGCGGAACTTGCGACTTTAGTAGATCACTTAATCTCCT
ATCCGTTAAATGAACAATTAAAATTCTTCATGGTAGCAGATCGTTTTATGTATTTAGCTGGCGAATTTGAAGATTACGAT
GCGTTCTATCAAGAAATGGATCAACATTTTGCAAAAGGGATTGTCGAAGTAGAAAAAGCAGCGGATAAATTTGGTCAAGA
ATTTGCGCTAAAACATCATCAAGACGACATTCATTATTTCGTTGGTGCTGGGAACCAGTGGGGTGCGACCTATTCTTACG
CAATGTGTTATTGGGAAGAGCAACACTGGATTAAAACAAAATCAATTGAGTCTCACGAGTTTTTCCATGGCATGTTTGAA
ATTGTAGAACGCGATACACCAGTTACCATTTATGTGACAGAAGATAGCCAGCGTTCTTTAAGCGAGCGAGTAGTTAATTT
CATTCCGCAAATCTGTGCCAATTATACAGTCATTGATGCAAAAGATTACGATATGCCGGGTATTTCCGCTAAATTCAGAG
GGGCGTTATCACCATTTATTATCCACGCAGTAAATAATCGCATTGACGTACACGTGGAAAAAATTAACTGTCACCCAATG
GAAATCAGAAGATATTATCGTCAGTTAGACTATTAA

Upstream 100 bases:

>100_bases
ATTTCCAAGAAAAAAGGAATTAATTTAGAAGTATCCGCTTTTCCGGAGTTTGACCAAATAACGAAATCCAAAATTTAATC
AAAGAAAGTAGGAATGAATA

Downstream 100 bases:

>100_bases
AAAGGCGGTTTGAAAAATGAAAGCAATTTTTGTTTGTACTCATGGAAATGCCGCAAAAGAACTCATTCAATCTGCAGAAA
TGATTTGCGGTAAACAAGAA

Product: opine catabolism protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MLKFDEQKVRENMEGALKLRPQINEVVDQIHDRGFSNICWLGIGGTYASAMQAVVHMKEKTALETFYENAAVFLTTGNKR
VTKDTLVVISSVTGSTQEVVDAVKKCNEIGATVFGFIDKAEAELATLVDHLISYPLNEQLKFFMVADRFMYLAGEFEDYD
AFYQEMDQHFAKGIVEVEKAADKFGQEFALKHHQDDIHYFVGAGNQWGATYSYAMCYWEEQHWIKTKSIESHEFFHGMFE
IVERDTPVTIYVTEDSQRSLSERVVNFIPQICANYTVIDAKDYDMPGISAKFRGALSPFIIHAVNNRIDVHVEKINCHPM
EIRRYYRQLDY

Sequences:

>Translated_331_residues
MLKFDEQKVRENMEGALKLRPQINEVVDQIHDRGFSNICWLGIGGTYASAMQAVVHMKEKTALETFYENAAVFLTTGNKR
VTKDTLVVISSVTGSTQEVVDAVKKCNEIGATVFGFIDKAEAELATLVDHLISYPLNEQLKFFMVADRFMYLAGEFEDYD
AFYQEMDQHFAKGIVEVEKAADKFGQEFALKHHQDDIHYFVGAGNQWGATYSYAMCYWEEQHWIKTKSIESHEFFHGMFE
IVERDTPVTIYVTEDSQRSLSERVVNFIPQICANYTVIDAKDYDMPGISAKFRGALSPFIIHAVNNRIDVHVEKINCHPM
EIRRYYRQLDY
>Mature_331_residues
MLKFDEQKVRENMEGALKLRPQINEVVDQIHDRGFSNICWLGIGGTYASAMQAVVHMKEKTALETFYENAAVFLTTGNKR
VTKDTLVVISSVTGSTQEVVDAVKKCNEIGATVFGFIDKAEAELATLVDHLISYPLNEQLKFFMVADRFMYLAGEFEDYD
AFYQEMDQHFAKGIVEVEKAADKFGQEFALKHHQDDIHYFVGAGNQWGATYSYAMCYWEEQHWIKTKSIESHEFFHGMFE
IVERDTPVTIYVTEDSQRSLSERVVNFIPQICANYTVIDAKDYDMPGISAKFRGALSPFIIHAVNNRIDVHVEKINCHPM
EIRRYYRQLDY

Specific function: Catalyzes the conversion of a range of fructosamine 6- phosphates to glucose 6-phosphate and a free amino acid [H]

COG id: COG2222

COG function: function code M; Predicted phosphosugar isomerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 SIS domains [H]

Homologues:

Organism=Escherichia coli, GI87082251, Length=316, Percent_Identity=23.1012658227848, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001347 [H]

Pfam domain/function: PF01380 SIS [H]

EC number: NA

Molecular weight: Translated: 38104; Mature: 38104

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKFDEQKVRENMEGALKLRPQINEVVDQIHDRGFSNICWLGIGGTYASAMQAVVHMKEK
CCCCCHHHHHHCCCCCEEECCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHH
TALETFYENAAVFLTTGNKRVTKDTLVVISSVTGSTQEVVDAVKKCNEIGATVFGFIDKA
HHHHHHHCCCEEEEEECCCEECHHHEEEEECCCCCHHHHHHHHHHHHHHCHHHHHHHHHH
EAELATLVDHLISYPLNEQLKFFMVADRFMYLAGEFEDYDAFYQEMDQHFAKGIVEVEKA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
ADKFGQEFALKHHQDDIHYFVGAGNQWGATYSYAMCYWEEQHWIKTKSIESHEFFHGMFE
HHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEEEECCCCCEECCCCCHHHHHHHHHH
IVERDTPVTIYVTEDSQRSLSERVVNFIPQICANYTVIDAKDYDMPGISAKFRGALSPFI
HHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHCCCCHHH
IHAVNNRIDVHVEKINCHPMEIRRYYRQLDY
HHHHCCEEEEEEEEECCCHHHHHHHHHHCCC
>Mature Secondary Structure
MLKFDEQKVRENMEGALKLRPQINEVVDQIHDRGFSNICWLGIGGTYASAMQAVVHMKEK
CCCCCHHHHHHCCCCCEEECCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHH
TALETFYENAAVFLTTGNKRVTKDTLVVISSVTGSTQEVVDAVKKCNEIGATVFGFIDKA
HHHHHHHCCCEEEEEECCCEECHHHEEEEECCCCCHHHHHHHHHHHHHHCHHHHHHHHHH
EAELATLVDHLISYPLNEQLKFFMVADRFMYLAGEFEDYDAFYQEMDQHFAKGIVEVEKA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
ADKFGQEFALKHHQDDIHYFVGAGNQWGATYSYAMCYWEEQHWIKTKSIESHEFFHGMFE
HHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEEEECCCCCEECCCCCHHHHHHHHHH
IVERDTPVTIYVTEDSQRSLSERVVNFIPQICANYTVIDAKDYDMPGISAKFRGALSPFI
HHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHCCCCHHH
IHAVNNRIDVHVEKINCHPMEIRRYYRQLDY
HHHHCCEEEEEEEEECCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]