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The map label for this gene is purK

Identifier: 226224377

GI number: 226224377

Start: 1832791

End: 1833915

Strand: Reverse

Name: purK

Synonym: Lm4b_01788

Alternate gene names: 226224377

Gene position: 1833915-1832791 (Counterclockwise)

Preceding gene: 226224378

Following gene: 226224376

Centisome position: 62.96

GC content: 37.69

Gene sequence:

>1125_bases
TTGGATAAAAAATTTTTACTGACAAATAGTACTATTGGTATTATTGGTGGCGGACAACTTGGACGTATGCTTGCTCTTGC
GGCGAAAGCGATGGGGTATCGTATTATTGTTCTTGATCCTACCGCTGATTGCCCAGCTGCTCAAGTAAGTGATGAACAAA
TTATTGCTGACTATGATGATAAAGTAGCATTACGCGAACTTTCCGAAAAAGCAGATGTAGTTACATATGAATTTGAAAAT
ATCGACTATGATGCTTTGAAAATGACGCAAAACTTAGTATCTGTCCCGCAAGGATCGGAATTGCTTTCGATTACACAGGA
TAGGATTTTGGAAAAAGCTTATCTGGAATCAGCTAATATTAACATTGCGCCGTATGCGGTTATAGTGGAACAAGATGAAA
TTGAAAATGAAATTAAAAGTATTGGATATCCTGCTGTTTTAAAAACGGCACAAGGTGGATACGACGGGAAAGGGCAAGTG
GTTATTCATGATGCAAGGGATATTGAAACAGCAGCGAGATTGTTGCGATACGGCTCTTGTGTACTAGAAGCTTGGATTCC
TTTTGAAAAAGAGATTTCTGTGGTTGTCGCTCGTAATTTAGATGGGCAAATAGAAACTTTTCCAGTTGCAGAAAATGTAC
ATGTAAATAATATTTTGCATACAACTACGGCTCCTGCAGATGTGGCAGATGACGTGCATGAGGAAGCGGAAGAAATTGCG
AGAAAGTTAGCAGATGTACTTCAATTATGTGGTGTTTTAGCAGTAGAAATGTTTGTGACAAAATCAGGGGCTATTTATGT
AAATGAACTTGCTCCAAGACCTCATAATTCTGGGCATTTTACAATTGAAGCTTGTTCTATTTCTCAGTTTACGCAGCATA
TTCGAGCGATTGTTGGATTGCCACTTGTAAAACCAGAACTCTTAAAACCAGCCTTAATGATTAATATTTTAGGGCAACAT
GTCGATGCAGTGAATAAACAAATGGTGAATTATCCACATTGGTTTGTACATTATTATGGAAAAAAAGAAGCGAAAATCAA
TCGCAAAATGGGTCATGTTACTGTGCTGACTGATGAACCAAAAGCGGTTATACAAGATTTAGAAGAAACGCAAATTTGGA
AGTAA

Upstream 100 bases:

>100_bases
GCGGCGCAAATTTTATCCATAACGGACGATGCAATTACTAACAGATTACAAAAAAGACGTGCTACACTAGAAGAAACAGT
TCTAGAAAGTAGTGATTATC

Downstream 100 bases:

>100_bases
CTGGAGGAATTTAAAAGGATGTTAGAACGTTATACTCGTAAAGAAATGGGCAACATTTGGACAGAACAAAATCGTTATCA
GGCTTGGCTAGAAGTGGAGA

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MDKKFLLTNSTIGIIGGGQLGRMLALAAKAMGYRIIVLDPTADCPAAQVSDEQIIADYDDKVALRELSEKADVVTYEFEN
IDYDALKMTQNLVSVPQGSELLSITQDRILEKAYLESANINIAPYAVIVEQDEIENEIKSIGYPAVLKTAQGGYDGKGQV
VIHDARDIETAARLLRYGSCVLEAWIPFEKEISVVVARNLDGQIETFPVAENVHVNNILHTTTAPADVADDVHEEAEEIA
RKLADVLQLCGVLAVEMFVTKSGAIYVNELAPRPHNSGHFTIEACSISQFTQHIRAIVGLPLVKPELLKPALMINILGQH
VDAVNKQMVNYPHWFVHYYGKKEAKINRKMGHVTVLTDEPKAVIQDLEETQIWK

Sequences:

>Translated_374_residues
MDKKFLLTNSTIGIIGGGQLGRMLALAAKAMGYRIIVLDPTADCPAAQVSDEQIIADYDDKVALRELSEKADVVTYEFEN
IDYDALKMTQNLVSVPQGSELLSITQDRILEKAYLESANINIAPYAVIVEQDEIENEIKSIGYPAVLKTAQGGYDGKGQV
VIHDARDIETAARLLRYGSCVLEAWIPFEKEISVVVARNLDGQIETFPVAENVHVNNILHTTTAPADVADDVHEEAEEIA
RKLADVLQLCGVLAVEMFVTKSGAIYVNELAPRPHNSGHFTIEACSISQFTQHIRAIVGLPLVKPELLKPALMINILGQH
VDAVNKQMVNYPHWFVHYYGKKEAKINRKMGHVTVLTDEPKAVIQDLEETQIWK
>Mature_374_residues
MDKKFLLTNSTIGIIGGGQLGRMLALAAKAMGYRIIVLDPTADCPAAQVSDEQIIADYDDKVALRELSEKADVVTYEFEN
IDYDALKMTQNLVSVPQGSELLSITQDRILEKAYLESANINIAPYAVIVEQDEIENEIKSIGYPAVLKTAQGGYDGKGQV
VIHDARDIETAARLLRYGSCVLEAWIPFEKEISVVVARNLDGQIETFPVAENVHVNNILHTTTAPADVADDVHEEAEEIA
RKLADVLQLCGVLAVEMFVTKSGAIYVNELAPRPHNSGHFTIEACSISQFTQHIRAIVGLPLVKPELLKPALMINILGQH
VDAVNKQMVNYPHWFVHYYGKKEAKINRKMGHVTVLTDEPKAVIQDLEETQIWK

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=359, Percent_Identity=33.1476323119777, Blast_Score=174, Evalue=6e-45,
Organism=Escherichia coli, GI1788155, Length=352, Percent_Identity=25.8522727272727, Blast_Score=112, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6324702, Length=371, Percent_Identity=33.9622641509434, Blast_Score=223, Evalue=5e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006115
- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp; PF03446 NAD_binding_2 [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 41398; Mature: 41398

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKKFLLTNSTIGIIGGGQLGRMLALAAKAMGYRIIVLDPTADCPAAQVSDEQIIADYDD
CCCEEEEECCEEEEEECCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEEECCCC
KVALRELSEKADVVTYEFENIDYDALKMTQNLVSVPQGSELLSITQDRILEKAYLESANI
HHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCC
NIAPYAVIVEQDEIENEIKSIGYPAVLKTAQGGYDGKGQVVIHDARDIETAARLLRYGSC
CEEEEEEEEECHHHHHHHHHCCCCHHEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHH
VLEAWIPFEKEISVVVARNLDGQIETFPVAENVHVNNILHTTTAPADVADDVHEEAEEIA
HHHHCCCCCCCEEEEEEECCCCCEEEECCCCCCEECCEEECCCCCHHHHHHHHHHHHHHH
RKLADVLQLCGVLAVEMFVTKSGAIYVNELAPRPHNSGHFTIEACSISQFTQHIRAIVGL
HHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEECCHHHHHHHHHHHHCC
PLVKPELLKPALMINILGQHVDAVNKQMVNYPHWFVHYYGKKEAKINRKMGHVTVLTDEP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHCCCCEEEEEECCC
KAVIQDLEETQIWK
HHHHHHHHHHCCCC
>Mature Secondary Structure
MDKKFLLTNSTIGIIGGGQLGRMLALAAKAMGYRIIVLDPTADCPAAQVSDEQIIADYDD
CCCEEEEECCEEEEEECCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEEECCCC
KVALRELSEKADVVTYEFENIDYDALKMTQNLVSVPQGSELLSITQDRILEKAYLESANI
HHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCC
NIAPYAVIVEQDEIENEIKSIGYPAVLKTAQGGYDGKGQVVIHDARDIETAARLLRYGSC
CEEEEEEEEECHHHHHHHHHCCCCHHEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHH
VLEAWIPFEKEISVVVARNLDGQIETFPVAENVHVNNILHTTTAPADVADDVHEEAEEIA
HHHHCCCCCCCEEEEEEECCCCCEEEECCCCCCEECCEEECCCCCHHHHHHHHHHHHHHH
RKLADVLQLCGVLAVEMFVTKSGAIYVNELAPRPHNSGHFTIEACSISQFTQHIRAIVGL
HHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEECCHHHHHHHHHHHHCC
PLVKPELLKPALMINILGQHVDAVNKQMVNYPHWFVHYYGKKEAKINRKMGHVTVLTDEP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHCCCCEEEEEECCC
KAVIQDLEETQIWK
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]