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The map label for this gene is purF

Identifier: 226224371

GI number: 226224371

Start: 1826118

End: 1827545

Strand: Reverse

Name: purF

Synonym: Lm4b_01782

Alternate gene names: 226224371

Gene position: 1827545-1826118 (Counterclockwise)

Preceding gene: 226224372

Following gene: 226224370

Centisome position: 62.74

GC content: 41.6

Gene sequence:

>1428_bases
ATGCTTGCTGAAGTAAAAGGACTTAACGAAGAATGTGGTATTTTTGGTATTTGGGATCATCCTAATGCAGCGGAAATCAC
CTATTATGGGTTGCATAGTTTACAACATCGCGGTCAAGAAGGTGCCGGAATTGTTTCGACAGACGGTGAAACACTAAAAG
GTCACCGAAATCTCGGCCTTTTAGCAGACGTATTTAAACATGGTGAACTAGACGATTTAAAAGGTAAAGCAGCCATTGGC
CACGTTCGATATGCGACAGCCGGTCAAAAAAACTTAGGAAACGTACAACCATTTTTATTTCACTTTCATAGTTCTTCTTT
AGCACTTGCGCATAATGGAAATTTGGTTAATGCGAAAAGTTTACGCCGTGAACTAGAAGAAGAAGGCGCTATTTTTCAAA
CGAGTTCGGATACAGAAGTGTTAGCGCATTTAATTAAACGTAGCCATACAGGTGATTTTGTGGAAGATTTAAAAGTTGCT
TTGAACAAGGTTAAGGGTGGCTTTGCGTACATGCTTCTGACGGAAGACACGATGTATGCGGCGCTTGACCCGAATGGTTT
TAGACCGCTTTCGATAGGTCGCATTGGTGATTCTTACGTAGTTGCTTCGGAAACATGTGCCTTTGAAACGGTTGGCGCTG
AGTTTGTTCGCGATGTGGAGCCAGGTGAACTAATTATTATTAATGATGACGGGCTTCGAATTGAAAAATTCACGGAAAAT
GTGACGCATTCGATTTGCAGTATGGAATACATTTATTTTGCGCGACCTGACTCTAATATTGCTGGAGTTAATGTCCACTC
GGCAAGAAAACGTTCTGGGAAAAGATTAGCAAAAGAGGCTTTTATTGATGCAGATGTGGTTACAGGTGTACCAGATTCCA
GTATTTCTGCAGCGATTGGTTATGCGGAGGAAGCTGGTCTTCCTTACGAACTTGGTCTCATCAAAAATAGATATGTGGCA
AGAACTTTTATCCAGCCATCACAAGAACTTAGGGAACAAGGCGTTAGAATGAAACTTTCTGCCGTGCGCGGGGTTGTGGA
AGGAAAACGCGTTGTCATGATTGATGATTCGATTGTTCGCGGGACGACAAGTAAACGCATTGTCCAGCTTTTACGTGAAG
CAGGAGCGGCGGAAGTTCATGTAAGAATCGCTTCTCCACCACTTGCTTATCCTTGTTTTTATGGAATTGATATCCAAACA
CGAAATGAATTAATCGCTTCTAATTATTCTGTTGATGAAATTTGTCGGATTATTGGGGCTGATTCTTTAGAGTATTTGAG
TGAAGAAGGTTTAGTAGATTCCATTGGCAGACCTTATCCGAACGAACCTTACGGGGGACTTTGTATGGCTTATTTCAACG
GCGATTACCCAACCCCTTTATATGATTATGAAGCGGAATATTTAGCGAGCTTAGAAGCAGAAAAATAA

Upstream 100 bases:

>100_bases
GTGTAGTAACCGAGGATGATACGATTCGTGTGAAACATAAGGAAGATCAAGTTACAGCGAAAACAACGGAATTACGATCC
ATTTGGGAAGGGGCTATTCC

Downstream 100 bases:

>100_bases
TGAGGAGTGACAAATCGAATGGCAGAAAATGCATATAGTAAAGCAGGCGTGGACGTAGAAGCTGGCTATCAAGTTGTAGA
ACGTATCAAAAAACATGTGG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MLAEVKGLNEECGIFGIWDHPNAAEITYYGLHSLQHRGQEGAGIVSTDGETLKGHRNLGLLADVFKHGELDDLKGKAAIG
HVRYATAGQKNLGNVQPFLFHFHSSSLALAHNGNLVNAKSLRRELEEEGAIFQTSSDTEVLAHLIKRSHTGDFVEDLKVA
LNKVKGGFAYMLLTEDTMYAALDPNGFRPLSIGRIGDSYVVASETCAFETVGAEFVRDVEPGELIIINDDGLRIEKFTEN
VTHSICSMEYIYFARPDSNIAGVNVHSARKRSGKRLAKEAFIDADVVTGVPDSSISAAIGYAEEAGLPYELGLIKNRYVA
RTFIQPSQELREQGVRMKLSAVRGVVEGKRVVMIDDSIVRGTTSKRIVQLLREAGAAEVHVRIASPPLAYPCFYGIDIQT
RNELIASNYSVDEICRIIGADSLEYLSEEGLVDSIGRPYPNEPYGGLCMAYFNGDYPTPLYDYEAEYLASLEAEK

Sequences:

>Translated_475_residues
MLAEVKGLNEECGIFGIWDHPNAAEITYYGLHSLQHRGQEGAGIVSTDGETLKGHRNLGLLADVFKHGELDDLKGKAAIG
HVRYATAGQKNLGNVQPFLFHFHSSSLALAHNGNLVNAKSLRRELEEEGAIFQTSSDTEVLAHLIKRSHTGDFVEDLKVA
LNKVKGGFAYMLLTEDTMYAALDPNGFRPLSIGRIGDSYVVASETCAFETVGAEFVRDVEPGELIIINDDGLRIEKFTEN
VTHSICSMEYIYFARPDSNIAGVNVHSARKRSGKRLAKEAFIDADVVTGVPDSSISAAIGYAEEAGLPYELGLIKNRYVA
RTFIQPSQELREQGVRMKLSAVRGVVEGKRVVMIDDSIVRGTTSKRIVQLLREAGAAEVHVRIASPPLAYPCFYGIDIQT
RNELIASNYSVDEICRIIGADSLEYLSEEGLVDSIGRPYPNEPYGGLCMAYFNGDYPTPLYDYEAEYLASLEAEK
>Mature_475_residues
MLAEVKGLNEECGIFGIWDHPNAAEITYYGLHSLQHRGQEGAGIVSTDGETLKGHRNLGLLADVFKHGELDDLKGKAAIG
HVRYATAGQKNLGNVQPFLFHFHSSSLALAHNGNLVNAKSLRRELEEEGAIFQTSSDTEVLAHLIKRSHTGDFVEDLKVA
LNKVKGGFAYMLLTEDTMYAALDPNGFRPLSIGRIGDSYVVASETCAFETVGAEFVRDVEPGELIIINDDGLRIEKFTEN
VTHSICSMEYIYFARPDSNIAGVNVHSARKRSGKRLAKEAFIDADVVTGVPDSSISAAIGYAEEAGLPYELGLIKNRYVA
RTFIQPSQELREQGVRMKLSAVRGVVEGKRVVMIDDSIVRGTTSKRIVQLLREAGAAEVHVRIASPPLAYPCFYGIDIQT
RNELIASNYSVDEICRIIGADSLEYLSEEGLVDSIGRPYPNEPYGGLCMAYFNGDYPTPLYDYEAEYLASLEAEK

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=469, Percent_Identity=42.6439232409382, Blast_Score=363, Evalue=1e-100,
Organism=Escherichia coli, GI1788651, Length=484, Percent_Identity=39.6694214876033, Blast_Score=305, Evalue=3e-84,
Organism=Escherichia coli, GI1790167, Length=215, Percent_Identity=31.6279069767442, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17554892, Length=485, Percent_Identity=40.2061855670103, Blast_Score=360, Evalue=1e-99,
Organism=Saccharomyces cerevisiae, GI6323958, Length=495, Percent_Identity=35.7575757575758, Blast_Score=250, Evalue=3e-67,
Organism=Drosophila melanogaster, GI24659598, Length=489, Percent_Identity=42.7402862985685, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24659604, Length=457, Percent_Identity=43.9824945295405, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI28573187, Length=497, Percent_Identity=41.4486921529175, Blast_Score=369, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 52149; Mature: 52149

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAEVKGLNEECGIFGIWDHPNAAEITYYGLHSLQHRGQEGAGIVSTDGETLKGHRNLGL
CCCCCCCCCCCCCEEEEECCCCCCEEEEEEHHHHHHCCCCCCEEEECCCCHHCCCCCCCH
LADVFKHGELDDLKGKAAIGHVRYATAGQKNLGNVQPFLFHFHSSSLALAHNGNLVNAKS
HHHHHHCCCCCCCCCCEEECEEEEECCCCCCCCCCCEEEEEEECCCEEEEECCCEECHHH
LRRELEEEGAIFQTSSDTEVLAHLIKRSHTGDFVEDLKVALNKVKGGFAYMLLTEDTMYA
HHHHHHHCCCEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCEEE
ALDPNGFRPLSIGRIGDSYVVASETCAFETVGAEFVRDVEPGELIIINDDGLRIEKFTEN
EECCCCCCEEEECCCCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEECCCCCEEHHHHHH
VTHSICSMEYIYFARPDSNIAGVNVHSARKRSGKRLAKEAFIDADVVTGVPDSSISAAIG
HHHHHHCEEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHCCCHHCCCCCCCCHHHHCC
YAEEAGLPYELGLIKNRYVARTFIQPSQELREQGVRMKLSAVRGVVEGKRVVMIDDSIVR
CHHHCCCCEEEHHHCCCHHHHHHCCCHHHHHHCCCEEEHHHHHHHHCCCEEEEECCCHHC
GTTSKRIVQLLREAGAAEVHVRIASPPLAYPCFYGIDIQTRNELIASNYSVDEICRIIGA
CCCHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEECEECCCCHHHHCCCCHHHHHHHHCC
DSLEYLSEEGLVDSIGRPYPNEPYGGLCMAYFNGDYPTPLYDYEAEYLASLEAEK
CHHHHHHHCCCHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHCCCCC
>Mature Secondary Structure
MLAEVKGLNEECGIFGIWDHPNAAEITYYGLHSLQHRGQEGAGIVSTDGETLKGHRNLGL
CCCCCCCCCCCCCEEEEECCCCCCEEEEEEHHHHHHCCCCCCEEEECCCCHHCCCCCCCH
LADVFKHGELDDLKGKAAIGHVRYATAGQKNLGNVQPFLFHFHSSSLALAHNGNLVNAKS
HHHHHHCCCCCCCCCCEEECEEEEECCCCCCCCCCCEEEEEEECCCEEEEECCCEECHHH
LRRELEEEGAIFQTSSDTEVLAHLIKRSHTGDFVEDLKVALNKVKGGFAYMLLTEDTMYA
HHHHHHHCCCEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCEEE
ALDPNGFRPLSIGRIGDSYVVASETCAFETVGAEFVRDVEPGELIIINDDGLRIEKFTEN
EECCCCCCEEEECCCCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEECCCCCEEHHHHHH
VTHSICSMEYIYFARPDSNIAGVNVHSARKRSGKRLAKEAFIDADVVTGVPDSSISAAIG
HHHHHHCEEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHCCCHHCCCCCCCCHHHHCC
YAEEAGLPYELGLIKNRYVARTFIQPSQELREQGVRMKLSAVRGVVEGKRVVMIDDSIVR
CHHHCCCCEEEHHHCCCHHHHHHCCCHHHHHHCCCEEEHHHHHHHHCCCEEEEECCCHHC
GTTSKRIVQLLREAGAAEVHVRIASPPLAYPCFYGIDIQTRNELIASNYSVDEICRIIGA
CCCHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEECEECCCCHHHHCCCCHHHHHHHHCC
DSLEYLSEEGLVDSIGRPYPNEPYGGLCMAYFNGDYPTPLYDYEAEYLASLEAEK
CHHHHHHHCCCHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]