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The map label for this gene is cobD

Identifier: 226223770

GI number: 226223770

Start: 1178437

End: 1179522

Strand: Direct

Name: cobD

Synonym: Lm4b_01174

Alternate gene names: 226223770

Gene position: 1178437-1179522 (Clockwise)

Preceding gene: 226223769

Following gene: 226223771

Centisome position: 40.46

GC content: 39.13

Gene sequence:

>1086_bases
GTGAAAATAACGACGGCTTCTCACGGTGGCAACTATAATGAACTAGCAAAACAACACGGATTAACGAAAGAAATGGTGCT
CGATTTCAGTGCGAATATTAATCCATTAGGAGTCCCAGCTAGTTTGAAACAAACGATAACAACAAATTTGGACAAACTGG
TAGAATATCCAGAACCAGATTACTTGGCGCTCCGTGCGCGAATTGCCTCGTTTCATCAACTCGACCTAGCGAATGTAATC
CCTGGAAACGGGGCGACAGAGCTGATTTTCGGAATAGCAAAAGTAACAAAGGCGCAAAAAGTACTCTTACTTGCGCCTAC
TTTTGCGGAATACGAACGTGCTTTTTTTGATGCCGAAATTGTTTATGCTGAGTTAACGAAAGAAACCAATTTTGCTGCGG
CGCAAATCGTACTAGAAATGCTTGAACAAGATACGGATATCGAAGCTGTTTGTTTGTGCAACCCCAATAATCCAACAGGA
CAACTCATCGCGCAACAAGAAATGATAAAAATTGCTGATTTATGTGAAAAGCGAAACATATATTTAATTATCGATGAGGC
TTTTATGGACTTTTTAGAAGAAAATGAAACAATTTCGATGATCAACTATTTAGAAAAATTTCCGCATTTAGCAATTATTC
GCGCCTTTACGAAGTTTTTCGCGATTCCAGGCTTAAGACTCGGTTATTTGCTGACAAAAAATGACTTATTAGCCGAAGCA
CTCATGCAAATGCGCGAACCCTGGTCGATTAATACATTTGCTGACTTAGCAGGGCAAATGCTATTAGAGGATGAAGCCTA
TATTAGACAAACATTCGATTGGATTAATGCGGAACGAGACTTTTTATACCGAGGCTTAAGTAAATTTTCAGCGCTTACCG
TGTACCGACCAAGTGTAAATTACATATTTTTCCACCTCGAAAAACCGTTTGATTTACGAAAAAAATTGCTTTTAAAAGGG
ATTTTTATCCGAAGTTGCGCTAATTACCGAGGTCTCACGGAGAATTATTACCGAGTGGCAGTGAAAAGTAGAGAAGATAA
TCGCCAACTCTTAACCGCACTTGAGGTGATTTTTAGTGGTAATTAA

Upstream 100 bases:

>100_bases
GCGGTCAAAGTGTGCATTATTCCAACCAATGAAGAACTAATGATTGCTCGTGATGTAGAAAAGTATGCAAAACAAACGAT
AAGTTAAGGAGCGATTTGAT

Downstream 100 bases:

>100_bases
AGCGAGGTGTCCGGCATCTTGTGGCGAACTTTTGCAAGGCTGGATTTTAGGCGGAGAGAAGCTAATTTCATACCCAATCA
ACTGGTATTCCGAAGTCACT

Product: threonine-phosphate decarboxylase

Products: NA

Alternate protein names: L-threonine-O-3-phosphate decarboxylase [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MKITTASHGGNYNELAKQHGLTKEMVLDFSANINPLGVPASLKQTITTNLDKLVEYPEPDYLALRARIASFHQLDLANVI
PGNGATELIFGIAKVTKAQKVLLLAPTFAEYERAFFDAEIVYAELTKETNFAAAQIVLEMLEQDTDIEAVCLCNPNNPTG
QLIAQQEMIKIADLCEKRNIYLIIDEAFMDFLEENETISMINYLEKFPHLAIIRAFTKFFAIPGLRLGYLLTKNDLLAEA
LMQMREPWSINTFADLAGQMLLEDEAYIRQTFDWINAERDFLYRGLSKFSALTVYRPSVNYIFFHLEKPFDLRKKLLLKG
IFIRSCANYRGLTENYYRVAVKSREDNRQLLTALEVIFSGN

Sequences:

>Translated_361_residues
MKITTASHGGNYNELAKQHGLTKEMVLDFSANINPLGVPASLKQTITTNLDKLVEYPEPDYLALRARIASFHQLDLANVI
PGNGATELIFGIAKVTKAQKVLLLAPTFAEYERAFFDAEIVYAELTKETNFAAAQIVLEMLEQDTDIEAVCLCNPNNPTG
QLIAQQEMIKIADLCEKRNIYLIIDEAFMDFLEENETISMINYLEKFPHLAIIRAFTKFFAIPGLRLGYLLTKNDLLAEA
LMQMREPWSINTFADLAGQMLLEDEAYIRQTFDWINAERDFLYRGLSKFSALTVYRPSVNYIFFHLEKPFDLRKKLLLKG
IFIRSCANYRGLTENYYRVAVKSREDNRQLLTALEVIFSGN
>Mature_361_residues
MKITTASHGGNYNELAKQHGLTKEMVLDFSANINPLGVPASLKQTITTNLDKLVEYPEPDYLALRARIASFHQLDLANVI
PGNGATELIFGIAKVTKAQKVLLLAPTFAEYERAFFDAEIVYAELTKETNFAAAQIVLEMLEQDTDIEAVCLCNPNNPTG
QLIAQQEMIKIADLCEKRNIYLIIDEAFMDFLEENETISMINYLEKFPHLAIIRAFTKFFAIPGLRLGYLLTKNDLLAEA
LMQMREPWSINTFADLAGQMLLEDEAYIRQTFDWINAERDFLYRGLSKFSALTVYRPSVNYIFFHLEKPFDLRKKLLLKG
IFIRSCANYRGLTENYYRVAVKSREDNRQLLTALEVIFSGN

Specific function: Decarboxylates L-threonine-O-3-phosphate to yield (R)-1- amino-2-propanol O-2-phosphate, the precursor for the linkage between the nucleotide loop and the corrin ring in cobalamin [H]

COG id: COG0079

COG function: function code E; Histidinol-phosphate/aromatic aminotransferase and cobyric acid decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI149944424, Length=260, Percent_Identity=25.7692307692308, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1788332, Length=336, Percent_Identity=28.2738095238095, Blast_Score=109, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6322075, Length=362, Percent_Identity=25.6906077348066, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004839
- InterPro:   IPR005860
- InterPro:   IPR004838
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =4.1.1.81 [H]

Molecular weight: Translated: 41171; Mature: 41171

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITTASHGGNYNELAKQHGLTKEMVLDFSANINPLGVPASLKQTITTNLDKLVEYPEPD
CEEECCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHCHHHHHCCCCCC
YLALRARIASFHQLDLANVIPGNGATELIFGIAKVTKAQKVLLLAPTFAEYERAFFDAEI
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCEEEEECCCHHHHHHHHHHHHH
VYAELTKETNFAAAQIVLEMLEQDTDIEAVCLCNPNNPTGQLIAQQEMIKIADLCEKRNI
HEEEHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCE
YLIIDEAFMDFLEENETISMINYLEKFPHLAIIRAFTKFFAIPGLRLGYLLTKNDLLAEA
EEEEHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHH
LMQMREPWSINTFADLAGQMLLEDEAYIRQTFDWINAERDFLYRGLSKFSALTVYRPSVN
HHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHEEEEECCCCC
YIFFHLEKPFDLRKKLLLKGIFIRSCANYRGLTENYYRVAVKSREDNRQLLTALEVIFSG
EEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCC
N
C
>Mature Secondary Structure
MKITTASHGGNYNELAKQHGLTKEMVLDFSANINPLGVPASLKQTITTNLDKLVEYPEPD
CEEECCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHCHHHHHCCCCCC
YLALRARIASFHQLDLANVIPGNGATELIFGIAKVTKAQKVLLLAPTFAEYERAFFDAEI
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCEEEEECCCHHHHHHHHHHHHH
VYAELTKETNFAAAQIVLEMLEQDTDIEAVCLCNPNNPTGQLIAQQEMIKIADLCEKRNI
HEEEHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCE
YLIIDEAFMDFLEENETISMINYLEKFPHLAIIRAFTKFFAIPGLRLGYLLTKNDLLAEA
EEEEHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHH
LMQMREPWSINTFADLAGQMLLEDEAYIRQTFDWINAERDFLYRGLSKFSALTVYRPSVN
HHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHEEEEECCCCC
YIFFHLEKPFDLRKKLLLKGIFIRSCANYRGLTENYYRVAVKSREDNRQLLTALEVIFSG
EEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCC
N
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]