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The map label for this gene is AckA2

Identifier: 226223769

GI number: 226223769

Start: 1177230

End: 1178423

Strand: Direct

Name: AckA2

Synonym: Lm4b_01173

Alternate gene names: 226223769

Gene position: 1177230-1178423 (Clockwise)

Preceding gene: 226223768

Following gene: 226223770

Centisome position: 40.42

GC content: 40.12

Gene sequence:

>1194_bases
ATGCACAAAATTATGGCGATAAACGCAGGGAGTTCTTCACTGAAATTCCAAATTTTTACGATGCCAGGAGAAGAAGTTTT
AGTTAAGGGCTTAATTGAAAGAATCGGTCTACCAGACGCCATTTTCAACATGTCCTTTCAAAATGAAAAAATCAAAGAAA
TACGAGCAATCAATAACCACGGGGAAGCAGTCGAGATTTTACTGGAGCAGCTGAAGGCGCATCAAGTAATCAATGATCTG
AGCGAAATTACAGGGGTCGGACATCGTGTTGCGCATGGCGGAGAAGATTTTGTTACGTCCTGTGTTGTAACAGATGAAGT
GGTGAAAGGGATTGAAGCTGTGACAAACCTTGCGCCGCTACATAATCCAGCGAATATCATCGGAATCAAAACTTTTCGTG
AATTGCTGCCAAATGCGGTTTCTGTTGCAGTATTTGATACCGCATTCCATCAAACGATTCCAGAAGAAAACTTTTTATAT
GCGCTTCCTTATGAACTTTATGAAAAACATCATATTCGAAAATACGGTTTTCATGGAACGAGTCATAAATATGTGGCTGG
AAAAGCAGCAGAAGTTCTCGAAAAACCTTTAGAAAAATTAAAAATTATTTCTTGTCATTTAGGCAATGGAGCGAGTGTTT
GTGCGATTGAAGCTGGGAAATCAGTGAATACTTCCATGGGCTTTACACCAAATGCTGGCTTGATGATGGGAACACGTTCT
GGTACAATTGACGCGACAATCATCCCGTATTTAGTGGATGAATTAGGCTATAGCTTGGATGAAGTGATGCATATGATGTC
TAGTGAATCAGGTGTCCTTGGCGTATCGGGAATTTCAAGTGATTTCAGAGATATCGAAATTGCAGCCAAAGAAGGTAATT
CACGCGCGCTATTAACACTTCGTATGTTTACTGGCCAAATATGCAACTACATCGGTGCTTATGCCTCCGCAATGAACGGT
TGCGACGCATTATTATTTACAGCGGGTGTGGGTGAAAATTCGCCATTAATCCGCCAAATGGTAACAGAACAGCTAAGTTA
TCTTGGTGTAACATGCCATGTGACAAAAAATAATGCGGGTGATATGATAATTAGCAATGATGATGAAGCGGTCAAAGTGT
GCATTATTCCAACCAATGAAGAACTAATGATTGCTCGTGATGTAGAAAAGTATGCAAAACAAACGATAAGTTAA

Upstream 100 bases:

>100_bases
TCTTAGCCATTGGACTATTTAACATTTTATTATAAAAAAGCATGGAGTAAACAAGAATGGCTTTGTTTACTCCTTTAAAA
ATGAGAAAGGACTGAGCTAT

Downstream 100 bases:

>100_bases
GGAGCGATTTGATGTGAAAATAACGACGGCTTCTCACGGTGGCAACTATAATGAACTAGCAAAACAACACGGATTAACGA
AAGAAATGGTGCTCGATTTC

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase 1

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MHKIMAINAGSSSLKFQIFTMPGEEVLVKGLIERIGLPDAIFNMSFQNEKIKEIRAINNHGEAVEILLEQLKAHQVINDL
SEITGVGHRVAHGGEDFVTSCVVTDEVVKGIEAVTNLAPLHNPANIIGIKTFRELLPNAVSVAVFDTAFHQTIPEENFLY
ALPYELYEKHHIRKYGFHGTSHKYVAGKAAEVLEKPLEKLKIISCHLGNGASVCAIEAGKSVNTSMGFTPNAGLMMGTRS
GTIDATIIPYLVDELGYSLDEVMHMMSSESGVLGVSGISSDFRDIEIAAKEGNSRALLTLRMFTGQICNYIGAYASAMNG
CDALLFTAGVGENSPLIRQMVTEQLSYLGVTCHVTKNNAGDMIISNDDEAVKVCIIPTNEELMIARDVEKYAKQTIS

Sequences:

>Translated_397_residues
MHKIMAINAGSSSLKFQIFTMPGEEVLVKGLIERIGLPDAIFNMSFQNEKIKEIRAINNHGEAVEILLEQLKAHQVINDL
SEITGVGHRVAHGGEDFVTSCVVTDEVVKGIEAVTNLAPLHNPANIIGIKTFRELLPNAVSVAVFDTAFHQTIPEENFLY
ALPYELYEKHHIRKYGFHGTSHKYVAGKAAEVLEKPLEKLKIISCHLGNGASVCAIEAGKSVNTSMGFTPNAGLMMGTRS
GTIDATIIPYLVDELGYSLDEVMHMMSSESGVLGVSGISSDFRDIEIAAKEGNSRALLTLRMFTGQICNYIGAYASAMNG
CDALLFTAGVGENSPLIRQMVTEQLSYLGVTCHVTKNNAGDMIISNDDEAVKVCIIPTNEELMIARDVEKYAKQTIS
>Mature_397_residues
MHKIMAINAGSSSLKFQIFTMPGEEVLVKGLIERIGLPDAIFNMSFQNEKIKEIRAINNHGEAVEILLEQLKAHQVINDL
SEITGVGHRVAHGGEDFVTSCVVTDEVVKGIEAVTNLAPLHNPANIIGIKTFRELLPNAVSVAVFDTAFHQTIPEENFLY
ALPYELYEKHHIRKYGFHGTSHKYVAGKAAEVLEKPLEKLKIISCHLGNGASVCAIEAGKSVNTSMGFTPNAGLMMGTRS
GTIDATIIPYLVDELGYSLDEVMHMMSSESGVLGVSGISSDFRDIEIAAKEGNSRALLTLRMFTGQICNYIGAYASAMNG
CDALLFTAGVGENSPLIRQMVTEQLSYLGVTCHVTKNNAGDMIISNDDEAVKVCIIPTNEELMIARDVEKYAKQTIS

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family

Homologues:

Organism=Escherichia coli, GI1788633, Length=399, Percent_Identity=44.3609022556391, Blast_Score=317, Evalue=8e-88,
Organism=Escherichia coli, GI145693184, Length=388, Percent_Identity=41.2371134020619, Blast_Score=311, Evalue=6e-86,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ACKA1_LISMF (Q720R2)

Other databases:

- EMBL:   AE017262
- RefSeq:   YP_013775.1
- ProteinModelPortal:   Q720R2
- SMR:   Q720R2
- STRING:   Q720R2
- GeneID:   2798341
- GenomeReviews:   AE017262_GR
- KEGG:   lmf:LMOf2365_1176
- TIGR:   LMOf2365_1176
- eggNOG:   COG0282
- HOGENOM:   HBG285863
- OMA:   WRYYAEL
- ProtClustDB:   CLSK2393564
- BioCyc:   LMON265669:LMOF2365_1176-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00020
- InterPro:   IPR000890
- InterPro:   IPR004372
- PANTHER:   PTHR21060
- PANTHER:   PTHR21060:SF11
- PIRSF:   PIRSF000722
- PRINTS:   PR00471
- TIGRFAMs:   TIGR00016

Pfam domain/function: PF00871 Acetate_kinase

EC number: =2.7.2.1

Molecular weight: Translated: 43150; Mature: 43150

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS01075 ACETATE_KINASE_1; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKIMAINAGSSSLKFQIFTMPGEEVLVKGLIERIGLPDAIFNMSFQNEKIKEIRAINNH
CCEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHCCC
GEAVEILLEQLKAHQVINDLSEITGVGHRVAHGGEDFVTSCVVTDEVVKGIEAVTNLAPL
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
HNPANIIGIKTFRELLPNAVSVAVFDTAFHQTIPEENFLYALPYELYEKHHIRKYGFHGT
CCCCCEEEHHHHHHHCCCHHEEEEHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHCCCCC
SHKYVAGKAAEVLEKPLEKLKIISCHLGNGASVCAIEAGKSVNTSMGFTPNAGLMMGTRS
CCCEECCHHHHHHHHHHHHHEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCEEEECCC
GTIDATIIPYLVDELGYSLDEVMHMMSSESGVLGVSGISSDFRDIEIAAKEGNSRALLTL
CCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCCCCCCEEEEEEECCCCEEEEEE
RMFTGQICNYIGAYASAMNGCDALLFTAGVGENSPLIRQMVTEQLSYLGVTCHVTKNNAG
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCEEEEEEECCCCC
DMIISNDDEAVKVCIIPTNEELMIARDVEKYAKQTIS
CEEEECCCCEEEEEEEECCCCEEEHHHHHHHHHHHCC
>Mature Secondary Structure
MHKIMAINAGSSSLKFQIFTMPGEEVLVKGLIERIGLPDAIFNMSFQNEKIKEIRAINNH
CCEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHCCC
GEAVEILLEQLKAHQVINDLSEITGVGHRVAHGGEDFVTSCVVTDEVVKGIEAVTNLAPL
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
HNPANIIGIKTFRELLPNAVSVAVFDTAFHQTIPEENFLYALPYELYEKHHIRKYGFHGT
CCCCCEEEHHHHHHHCCCHHEEEEHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHCCCCC
SHKYVAGKAAEVLEKPLEKLKIISCHLGNGASVCAIEAGKSVNTSMGFTPNAGLMMGTRS
CCCEECCHHHHHHHHHHHHHEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCEEEECCC
GTIDATIIPYLVDELGYSLDEVMHMMSSESGVLGVSGISSDFRDIEIAAKEGNSRALLTL
CCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCCCCCCEEEEEEECCCCEEEEEE
RMFTGQICNYIGAYASAMNGCDALLFTAGVGENSPLIRQMVTEQLSYLGVTCHVTKNNAG
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCEEEEEEECCCCC
DMIISNDDEAVKVCIIPTNEELMIARDVEKYAKQTIS
CEEEECCCCEEEEEEEECCCCEEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA