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The map label for this gene is glpF

Identifier: 226223768

GI number: 226223768

Start: 1176460

End: 1177164

Strand: Direct

Name: glpF

Synonym: Lm4b_01172

Alternate gene names: 226223768

Gene position: 1176460-1177164 (Clockwise)

Preceding gene: 226223767

Following gene: 226223769

Centisome position: 40.39

GC content: 40.14

Gene sequence:

>705_bases
ATGTCAGCATATTTGGCGGAATTTATTGGAACGATGGTTTTAATTATGTTTGGGAACGGCCTTTTAGCAGGATTGACATT
AAATAAATCATTATCTCAAGGAGCAAACTGGGTAGTTGTTACATTTGGTTGGGGTTTTGCGGTAATGATAGGGATTTATG
TCGCAGGAGCATATAGCGGGGCGCATTTAAATCCAGCTGTAACTATTGCGCTTGCAGTTGGAGGATCATTCCCTTGGGCA
GATGTGGTGCCATATATTATCGCGCAAATCGCCGGAGCATTTGTCGGAGCATCCATCGTTATTTTACACTACTATCCGCA
TTTTAAAGCGACTCCCCCAGAAATTGATACACATGGTATTTTTTCCACAGGACCAGCGATTAGAAATACACCTTTCAATT
TAATCAGTGAAATTATCGCAACATTTGCTTTCATTTTTGGTTTACTTATGATTGGAGCGAACAGTTTTACAGATGGTTTA
AATCCATTAATCCTTGGCTTTCTAGTAGTTGCCATCGGAATGAGTTTTGGACCAACGACAGGTTATGCGATTAACCCAGC
TCGTGATTTAGGACCAAGACTTGCTTACTTTTTACTACCAGTACCAAATAAAAGTGGGTCAGACTGGCGTTACGCTTGGA
TTCCAATTGTCGGACCAATCATTGGTGGTCTCTTAGCCATTGGACTATTTAACATTTTATTATAA

Upstream 100 bases:

>100_bases
CGCTTGCTTACCAACTAGTCCTACGACACCCTCACATCAAGAACTAAAAGAAATTTTAGAACAAATAATTTAGAATTTAC
ATTAAAAAGGAGGATAAACT

Downstream 100 bases:

>100_bases
AAAAGCATGGAGTAAACAAGAATGGCTTTGTTTACTCCTTTAAAAATGAGAAAGGACTGAGCTATATGCACAAAATTATG
GCGATAAACGCAGGGAGTTC

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MSAYLAEFIGTMVLIMFGNGLLAGLTLNKSLSQGANWVVVTFGWGFAVMIGIYVAGAYSGAHLNPAVTIALAVGGSFPWA
DVVPYIIAQIAGAFVGASIVILHYYPHFKATPPEIDTHGIFSTGPAIRNTPFNLISEIIATFAFIFGLLMIGANSFTDGL
NPLILGFLVVAIGMSFGPTTGYAINPARDLGPRLAYFLLPVPNKSGSDWRYAWIPIVGPIIGGLLAIGLFNILL

Sequences:

>Translated_234_residues
MSAYLAEFIGTMVLIMFGNGLLAGLTLNKSLSQGANWVVVTFGWGFAVMIGIYVAGAYSGAHLNPAVTIALAVGGSFPWA
DVVPYIIAQIAGAFVGASIVILHYYPHFKATPPEIDTHGIFSTGPAIRNTPFNLISEIIATFAFIFGLLMIGANSFTDGL
NPLILGFLVVAIGMSFGPTTGYAINPARDLGPRLAYFLLPVPNKSGSDWRYAWIPIVGPIIGGLLAIGLFNILL
>Mature_233_residues
SAYLAEFIGTMVLIMFGNGLLAGLTLNKSLSQGANWVVVTFGWGFAVMIGIYVAGAYSGAHLNPAVTIALAVGGSFPWAD
VVPYIIAQIAGAFVGASIVILHYYPHFKATPPEIDTHGIFSTGPAIRNTPFNLISEIIATFAFIFGLLMIGANSFTDGLN
PLILGFLVVAIGMSFGPTTGYAINPARDLGPRLAYFLLPVPNKSGSDWRYAWIPIVGPIIGGLLAIGLFNILL

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=248, Percent_Identity=36.2903225806452, Blast_Score=126, Evalue=1e-29,
Organism=Homo sapiens, GI4502187, Length=245, Percent_Identity=35.5102040816327, Blast_Score=116, Evalue=1e-26,
Organism=Homo sapiens, GI22538420, Length=245, Percent_Identity=33.8775510204082, Blast_Score=114, Evalue=6e-26,
Organism=Homo sapiens, GI157266307, Length=248, Percent_Identity=35.4838709677419, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI4755125, Length=243, Percent_Identity=31.2757201646091, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI4502181, Length=243, Percent_Identity=31.2757201646091, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI310133356, Length=220, Percent_Identity=31.3636363636364, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI310114181, Length=220, Percent_Identity=31.3636363636364, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1790362, Length=243, Percent_Identity=37.8600823045267, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1787101, Length=196, Percent_Identity=32.1428571428571, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71992966, Length=248, Percent_Identity=31.8548387096774, Blast_Score=113, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI71994009, Length=232, Percent_Identity=31.0344827586207, Blast_Score=110, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17531431, Length=244, Percent_Identity=32.7868852459016, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17531429, Length=244, Percent_Identity=32.7868852459016, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17533613, Length=232, Percent_Identity=33.1896551724138, Blast_Score=103, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI32564052, Length=232, Percent_Identity=33.1896551724138, Blast_Score=103, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17544068, Length=249, Percent_Identity=33.3333333333333, Blast_Score=98, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71992961, Length=238, Percent_Identity=31.0924369747899, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17558372, Length=238, Percent_Identity=27.7310924369748, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71993722, Length=237, Percent_Identity=27.4261603375527, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6321054, Length=210, Percent_Identity=40, Blast_Score=126, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6322985, Length=284, Percent_Identity=28.169014084507, Blast_Score=89, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24652747, Length=226, Percent_Identity=29.646017699115, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI45551084, Length=226, Percent_Identity=29.646017699115, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 24708; Mature: 24577

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAYLAEFIGTMVLIMFGNGLLAGLTLNKSLSQGANWVVVTFGWGFAVMIGIYVAGAYSG
CHHHHHHHHHHHHHHHHCCCEEEEEEECHHHHCCCCEEEEEECHHHHHHHHHHHHHCCCC
AHLNPAVTIALAVGGSFPWADVVPYIIAQIAGAFVGASIVILHYYPHFKATPPEIDTHGI
CCCCHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCC
FSTGPAIRNTPFNLISEIIATFAFIFGLLMIGANSFTDGLNPLILGFLVVAIGMSFGPTT
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
GYAINPARDLGPRLAYFLLPVPNKSGSDWRYAWIPIVGPIIGGLLAIGLFNILL
CCEECCHHHHCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SAYLAEFIGTMVLIMFGNGLLAGLTLNKSLSQGANWVVVTFGWGFAVMIGIYVAGAYSG
HHHHHHHHHHHHHHHHCCCEEEEEEECHHHHCCCCEEEEEECHHHHHHHHHHHHHCCCC
AHLNPAVTIALAVGGSFPWADVVPYIIAQIAGAFVGASIVILHYYPHFKATPPEIDTHGI
CCCCHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCC
FSTGPAIRNTPFNLISEIIATFAFIFGLLMIGANSFTDGLNPLILGFLVVAIGMSFGPTT
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
GYAINPARDLGPRLAYFLLPVPNKSGSDWRYAWIPIVGPIIGGLLAIGLFNILL
CCEECCHHHHCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8436953; 9579061; 9384377; 2127799 [H]