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The map label for this gene is adh1 [H]

Identifier: 226223767

GI number: 226223767

Start: 1175314

End: 1176432

Strand: Direct

Name: adh1 [H]

Synonym: Lm4b_01171

Alternate gene names: 226223767

Gene position: 1175314-1176432 (Clockwise)

Preceding gene: 226223766

Following gene: 226223768

Centisome position: 40.35

GC content: 40.3

Gene sequence:

>1119_bases
ATGAACAGCTTTCAAATTAAAACAAAAGTAGCTTTTGGTACGAATAGTTTACAAGTTTTAAAAGAAATTAAAAATAAAAA
TGTCTGGATTATCTGTGACCGTTTTTTGGCTGATGGGGAAGGGCTTCAAGGTTTAATTGGACAATTAGATACATCCAATA
ATGTGCATATTTTTACCGATGTCGTACCTGATCCACCTATTTCCAAAGTAGCTGGAGGGGTTAGTGAGGCAGGCAAAATC
CAACCGCAAGTCATGATTGCTTTTGGTGGTGGTTCAGCGATTGATACGGCTAAAGGAATCTACTATTTCGCCAAACGTTT
GGAAAAAATCAATATCAGTACTTTTATTGCAATTCCAACCACCAGCGGTACGGGTTCAGAAGTTACAGCCGCAACCGTTA
TCACTGATCCAACAACGAAAATTAAATATCCGCTTTTCTTAGATGAACTAATTCCGGATATGGCTATTTTGGATGCGCAA
CTGGTTGTTACGGTACCTCCAGCTATTACAGCGAACACAGGGATGGACGTCTTAACACACGCAATTGAAGCTTATGTCTC
TAAAGATGCAAGCGATTATACCGATGCTCTTGGTGAAAAAAGTGTGCAATTAACGTTACGTTTCTTAACGAGCTGTTATG
ATGATGGTCGTAATTTAGCGAATCGAGAAAAAATGCATAATGCTTCTACTATGGCTGGAATGGCTTTTAACTGCGCCAAT
TTAGGCTTGAATCACAGTATTGCGCATCAACTTGGTGCACAGTTCCACGTACCACACGGGCTAGCCAATGCGATCTTACT
AGATGCAGTGATTCGTTTTAACGCCTTTAAAAATCGTGAAACAGAACAAAAATATGCAGAAATGGCACGCATTTGTGGCA
TTGCTTCCAGATCAGACTCGAACGAAACAGCTGTCCGCTTACTTCGCGAAAGAATTGTAGCGATGATGGAACATATGCAA
ATGCCGCGCACATTGACAGACGCCGGTGTAGCAAAAGAAAAAGTCTATGCAAAAATGGACGAAATTGCAACGAATGCACT
GAAAGACGCTTGCTTACCAACTAGTCCTACGACACCCTCACATCAAGAACTAAAAGAAATTTTAGAACAAATAATTTAG

Upstream 100 bases:

>100_bases
GCCTACCGGAGAAGGAACCACTACAGCGCGCCATTTTGCTAGACGTCGCCGTTGTGTTTTAACAGATGGTTTTTCGATTC
GTTAAGAGGAGGCAAACTGA

Downstream 100 bases:

>100_bases
AATTTACATTAAAAAGGAGGATAAACTATGTCAGCATATTTGGCGGAATTTATTGGAACGATGGTTTTAATTATGTTTGG
GAACGGCCTTTTAGCAGGAT

Product: NADPH-dependent butanol dehydrogenase

Products: Acetyl-CoA; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: BDH [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MNSFQIKTKVAFGTNSLQVLKEIKNKNVWIICDRFLADGEGLQGLIGQLDTSNNVHIFTDVVPDPPISKVAGGVSEAGKI
QPQVMIAFGGGSAIDTAKGIYYFAKRLEKINISTFIAIPTTSGTGSEVTAATVITDPTTKIKYPLFLDELIPDMAILDAQ
LVVTVPPAITANTGMDVLTHAIEAYVSKDASDYTDALGEKSVQLTLRFLTSCYDDGRNLANREKMHNASTMAGMAFNCAN
LGLNHSIAHQLGAQFHVPHGLANAILLDAVIRFNAFKNRETEQKYAEMARICGIASRSDSNETAVRLLRERIVAMMEHMQ
MPRTLTDAGVAKEKVYAKMDEIATNALKDACLPTSPTTPSHQELKEILEQII

Sequences:

>Translated_372_residues
MNSFQIKTKVAFGTNSLQVLKEIKNKNVWIICDRFLADGEGLQGLIGQLDTSNNVHIFTDVVPDPPISKVAGGVSEAGKI
QPQVMIAFGGGSAIDTAKGIYYFAKRLEKINISTFIAIPTTSGTGSEVTAATVITDPTTKIKYPLFLDELIPDMAILDAQ
LVVTVPPAITANTGMDVLTHAIEAYVSKDASDYTDALGEKSVQLTLRFLTSCYDDGRNLANREKMHNASTMAGMAFNCAN
LGLNHSIAHQLGAQFHVPHGLANAILLDAVIRFNAFKNRETEQKYAEMARICGIASRSDSNETAVRLLRERIVAMMEHMQ
MPRTLTDAGVAKEKVYAKMDEIATNALKDACLPTSPTTPSHQELKEILEQII
>Mature_372_residues
MNSFQIKTKVAFGTNSLQVLKEIKNKNVWIICDRFLADGEGLQGLIGQLDTSNNVHIFTDVVPDPPISKVAGGVSEAGKI
QPQVMIAFGGGSAIDTAKGIYYFAKRLEKINISTFIAIPTTSGTGSEVTAATVITDPTTKIKYPLFLDELIPDMAILDAQ
LVVTVPPAITANTGMDVLTHAIEAYVSKDASDYTDALGEKSVQLTLRFLTSCYDDGRNLANREKMHNASTMAGMAFNCAN
LGLNHSIAHQLGAQFHVPHGLANAILLDAVIRFNAFKNRETEQKYAEMARICGIASRSDSNETAVRLLRERIVAMMEHMQ
MPRTLTDAGVAKEKVYAKMDEIATNALKDACLPTSPTTPSHQELKEILEQII

Specific function: This enzyme has activity using butanol and ethanol as substrates [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=369, Percent_Identity=23.3062330623306, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1787493, Length=383, Percent_Identity=36.0313315926893, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI87082107, Length=359, Percent_Identity=35.933147632312, Blast_Score=187, Evalue=7e-49,
Organism=Escherichia coli, GI48994951, Length=369, Percent_Identity=34.4173441734417, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1789163, Length=384, Percent_Identity=32.2916666666667, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1789386, Length=379, Percent_Identity=25.065963060686, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17537053, Length=379, Percent_Identity=23.7467018469657, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6321181, Length=376, Percent_Identity=34.3085106382979, Blast_Score=182, Evalue=8e-47,
Organism=Drosophila melanogaster, GI24657991, Length=424, Percent_Identity=23.5849056603774, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.1.1; 1.2.1.10 [C]

Molecular weight: Translated: 40444; Mature: 40444

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSFQIKTKVAFGTNSLQVLKEIKNKNVWIICDRFLADGEGLQGLIGQLDTSNNVHIFTD
CCCEEEEEEEEECCCHHHHHHHHHCCCEEEEEHHHHCCCCCHHHHHHCCCCCCCEEEEEE
VVPDPPISKVAGGVSEAGKIQPQVMIAFGGGSAIDTAKGIYYFAKRLEKINISTFIAIPT
CCCCCCHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEC
TSGTGSEVTAATVITDPTTKIKYPLFLDELIPDMAILDAQLVVTVPPAITANTGMDVLTH
CCCCCCCEEEEEEEECCCCCEECCHHHHHHCCCHHHHCCEEEEEECCCCCCCCCHHHHHH
AIEAYVSKDASDYTDALGEKSVQLTLRFLTSCYDDGRNLANREKMHNASTMAGMAFNCAN
HHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LGLNHSIAHQLGAQFHVPHGLANAILLDAVIRFNAFKNRETEQKYAEMARICGIASRSDS
CCCCHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
NETAVRLLRERIVAMMEHMQMPRTLTDAGVAKEKVYAKMDEIATNALKDACLPTSPTTPS
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
HQELKEILEQII
HHHHHHHHHHHC
>Mature Secondary Structure
MNSFQIKTKVAFGTNSLQVLKEIKNKNVWIICDRFLADGEGLQGLIGQLDTSNNVHIFTD
CCCEEEEEEEEECCCHHHHHHHHHCCCEEEEEHHHHCCCCCHHHHHHCCCCCCCEEEEEE
VVPDPPISKVAGGVSEAGKIQPQVMIAFGGGSAIDTAKGIYYFAKRLEKINISTFIAIPT
CCCCCCHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEC
TSGTGSEVTAATVITDPTTKIKYPLFLDELIPDMAILDAQLVVTVPPAITANTGMDVLTH
CCCCCCCEEEEEEEECCCCCEECCHHHHHHCCCHHHHCCEEEEEECCCCCCCCCHHHHHH
AIEAYVSKDASDYTDALGEKSVQLTLRFLTSCYDDGRNLANREKMHNASTMAGMAFNCAN
HHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LGLNHSIAHQLGAQFHVPHGLANAILLDAVIRFNAFKNRETEQKYAEMARICGIASRSDS
CCCCHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
NETAVRLLRERIVAMMEHMQMPRTLTDAGVAKEKVYAKMDEIATNALKDACLPTSPTTPS
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
HQELKEILEQII
HHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 1000 [C]

Specific activity: NA

Km value (mM): 0.15 {ethanol}} 7.05 {acetaldehyde}} 0.1 {acetaldehyde}} [C]

Substrates: Acetaldehyde; Coenzyme A; Nicotinamide adenine dinucleotide [C]

Specific reaction: Acetaldehyde + Coenzyme A + Nicotinamide adenine dinucleotide --> Acetyl-CoA + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: 8-Hydroxy quinoline-5-sulfonic acid; Dipicolinic acid; EDTA; Ethanol; GSH; Heptafluorbutanol; Imidazole; Iodoacetamide; Iodoacetate; Isobutyramide EDTA; NAD+; NADH; NEM; o-Phenanthroline of Zn2+; PCMB; Pyrazole; Substituted pyrazoles 4-iodo-; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2673928 [H]