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The map label for this gene is thiF [H]

Identifier: 226223667

GI number: 226223667

Start: 1076898

End: 1077896

Strand: Reverse

Name: thiF [H]

Synonym: Lm4b_01069

Alternate gene names: 226223667

Gene position: 1077896-1076898 (Counterclockwise)

Preceding gene: 226223669

Following gene: 226223666

Centisome position: 37.01

GC content: 37.84

Gene sequence:

>999_bases
TTGGAACGTTATGATAGACAGATGCGTGTTAAGAACATCGGAAAAGTTGGTCAAAAAAAGTTACTCACGAAAACGATTTT
GATTGTTGGCGTTGGCGCGATTGGTTCATATGCCGCAGAAATTTGTGCACGAATGGGCTTTGGAAAGTTAATTTTAATTG
ATCGTGACTATGTGGAGCTAAGTAACTTGCAGCGCCAATCGCTTTTCACGGAACAAGATGCATTAGACAAACAAGCAAAA
GCGTATGCGGCATCCAAAGCGTTACAATTAATTAATAGCGATATTGAAATCGAATATATAGTTGATGATGCGAACGCAAC
AAGTTTAACGCCTTATGCCGGAGCAATTGATTACATACTTGATTGTACAGATAATTTTATGACGCGCGACTTTTTAAATC
AGTTTTGTTTCAGCCATCAAATTCCTTGGATTTTCACCTCGTGTGCTGGAAATTATGCGAATCTCATGCCAATTATTCCA
CCCGACTCTGCTTGTTTACACTGTTTACTTGGTGATTTTCCGCAAACCAACGCAGCAAGTTGCGATATTATCGGTGTGGA
TGGTGCGCTGATTCCGATTGTCGCGGGCATGCAAGTTTCTTTACTTACACAAATGATAATCAATCCTGATTTCAAATCAA
ATACCTACTATCAACTAGATAATTGGCAATTTTCTTTCCGGTCTCTTGAAGTCAAGAAACGCCCCGATTGCTCTGGTTGC
GCAACTGGAAAAATGATTTCAGAAGTGCCTTTTTCCGAGCAAACAGTCGCTCTTTGCGGAAGAGATACAGTACAATTTCG
TCTGCCAAATAAAAGCAATTATCACGAAATTAAACAAATGCTGACGGAACAAAAAATTATTTATAATGAAAATCCCGCTT
TACTCAGTTTCCAATACGAAAAATTTCAATTTGTTATTTTCAAAAATGGACGCGTGTTACTTCATGGGACAGAAAATATA
ACAGAAGCGAAAAAAACATATCACTTATTTTTCAATTAA

Upstream 100 bases:

>100_bases
AAATGATGCCATACTAAGATTTCAAGAATAAATTAAAACCTTACAAGTAAGCGCTTTATGTGTTTTTCTGGTAAAATAAG
ATTGGAAAGAAGGTGGAAAA

Downstream 100 bases:

>100_bases
AGGAGGCAGTTATGGAACAAAAAACATTTATTAAGGTAACTTGTAGTATTTTAACGATTAGTGATACAAGGAATTTGGAT
ACGGATACTAGTGGTCAACT

Product: molybdopterin biosynthesis protein MoeB

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MERYDRQMRVKNIGKVGQKKLLTKTILIVGVGAIGSYAAEICARMGFGKLILIDRDYVELSNLQRQSLFTEQDALDKQAK
AYAASKALQLINSDIEIEYIVDDANATSLTPYAGAIDYILDCTDNFMTRDFLNQFCFSHQIPWIFTSCAGNYANLMPIIP
PDSACLHCLLGDFPQTNAASCDIIGVDGALIPIVAGMQVSLLTQMIINPDFKSNTYYQLDNWQFSFRSLEVKKRPDCSGC
ATGKMISEVPFSEQTVALCGRDTVQFRLPNKSNYHEIKQMLTEQKIIYNENPALLSFQYEKFQFVIFKNGRVLLHGTENI
TEAKKTYHLFFN

Sequences:

>Translated_332_residues
MERYDRQMRVKNIGKVGQKKLLTKTILIVGVGAIGSYAAEICARMGFGKLILIDRDYVELSNLQRQSLFTEQDALDKQAK
AYAASKALQLINSDIEIEYIVDDANATSLTPYAGAIDYILDCTDNFMTRDFLNQFCFSHQIPWIFTSCAGNYANLMPIIP
PDSACLHCLLGDFPQTNAASCDIIGVDGALIPIVAGMQVSLLTQMIINPDFKSNTYYQLDNWQFSFRSLEVKKRPDCSGC
ATGKMISEVPFSEQTVALCGRDTVQFRLPNKSNYHEIKQMLTEQKIIYNENPALLSFQYEKFQFVIFKNGRVLLHGTENI
TEAKKTYHLFFN
>Mature_332_residues
MERYDRQMRVKNIGKVGQKKLLTKTILIVGVGAIGSYAAEICARMGFGKLILIDRDYVELSNLQRQSLFTEQDALDKQAK
AYAASKALQLINSDIEIEYIVDDANATSLTPYAGAIDYILDCTDNFMTRDFLNQFCFSHQIPWIFTSCAGNYANLMPIIP
PDSACLHCLLGDFPQTNAASCDIIGVDGALIPIVAGMQVSLLTQMIINPDFKSNTYYQLDNWQFSFRSLEVKKRPDCSGC
ATGKMISEVPFSEQTVALCGRDTVQFRLPNKSNYHEIKQMLTEQKIIYNENPALLSFQYEKFQFVIFKNGRVLLHGTENI
TEAKKTYHLFFN

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=146, Percent_Identity=39.7260273972603, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI13376212, Length=259, Percent_Identity=30.5019305019305, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI38327032, Length=255, Percent_Identity=30.1960784313725, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI87082356, Length=242, Percent_Identity=32.2314049586777, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1787048, Length=246, Percent_Identity=32.520325203252, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1789177, Length=127, Percent_Identity=30.7086614173228, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17540406, Length=310, Percent_Identity=28.7096774193548, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI193203301, Length=167, Percent_Identity=26.9461077844311, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI86565431, Length=193, Percent_Identity=27.979274611399, Blast_Score=68, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI86565433, Length=193, Percent_Identity=27.979274611399, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17508819, Length=182, Percent_Identity=32.4175824175824, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320598, Length=178, Percent_Identity=31.4606741573034, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321903, Length=262, Percent_Identity=28.2442748091603, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6322639, Length=202, Percent_Identity=26.7326732673267, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24582879, Length=268, Percent_Identity=31.3432835820896, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24641311, Length=220, Percent_Identity=31.3636363636364, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI28573937, Length=192, Percent_Identity=27.0833333333333, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 37497; Mature: 37497

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERYDRQMRVKNIGKVGQKKLLTKTILIVGVGAIGSYAAEICARMGFGKLILIDRDYVEL
CCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHCCCCEEEEEECCHHHH
SNLQRQSLFTEQDALDKQAKAYAASKALQLINSDIEIEYIVDDANATSLTPYAGAIDYIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHH
DCTDNFMTRDFLNQFCFSHQIPWIFTSCAGNYANLMPIIPPDSACLHCLLGDFPQTNAAS
HCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEECCCCHHHHHHHHCCCCCCCCCC
CDIIGVDGALIPIVAGMQVSLLTQMIINPDFKSNTYYQLDNWQFSFRSLEVKKRPDCSGC
EEEEECCCHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEECCCEEEEEEEEECCCCCCCCC
ATGKMISEVPFSEQTVALCGRDTVQFRLPNKSNYHEIKQMLTEQKIIYNENPALLSFQYE
CCCCHHHCCCCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHCCEEECCCCCEEEEEEC
KFQFVIFKNGRVLLHGTENITEAKKTYHLFFN
EEEEEEEECCEEEEECCCCHHHHHHEEEEEEC
>Mature Secondary Structure
MERYDRQMRVKNIGKVGQKKLLTKTILIVGVGAIGSYAAEICARMGFGKLILIDRDYVEL
CCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHCCCCEEEEEECCHHHH
SNLQRQSLFTEQDALDKQAKAYAASKALQLINSDIEIEYIVDDANATSLTPYAGAIDYIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHH
DCTDNFMTRDFLNQFCFSHQIPWIFTSCAGNYANLMPIIPPDSACLHCLLGDFPQTNAAS
HCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEECCCCHHHHHHHHCCCCCCCCCC
CDIIGVDGALIPIVAGMQVSLLTQMIINPDFKSNTYYQLDNWQFSFRSLEVKKRPDCSGC
EEEEECCCHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEECCCEEEEEEEEECCCCCCCCC
ATGKMISEVPFSEQTVALCGRDTVQFRLPNKSNYHEIKQMLTEQKIIYNENPALLSFQYE
CCCCHHHCCCCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHCCEEECCCCCEEEEEEC
KFQFVIFKNGRVLLHGTENITEAKKTYHLFFN
EEEEEEEECCEEEEECCCCHHHHHHEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]