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The map label for this gene is def

Identifier: 226223669

GI number: 226223669

Start: 1078631

End: 1079182

Strand: Reverse

Name: def

Synonym: Lm4b_01071

Alternate gene names: 226223669

Gene position: 1079182-1078631 (Counterclockwise)

Preceding gene: 226223678

Following gene: 226223667

Centisome position: 37.05

GC content: 39.86

Gene sequence:

>552_bases
ATGCTTACAATGGACGATATTGTACGAGAAGGTCATCCAGCACTTCGAGAAGTTGCGACAGAAGTGACTTTCCCGCTTTC
AGATGAAGAAAAAAAATTAGGACGCGAGATGCTCGAATTCCTGATTAACAGTCAAGATGAAGAGTTAGCAGAAAAATATG
GGTTGCGTGGTGGCGTTGGAATTGCAGCTCCACAACTCGCTGTGACTAAACGTTTCCTAGCTATTCATGTACATGATGAA
AAAGATCGTCTTTATAGTTATGTGCTTTATAATCCAAAAATTCGTAGCCATTCTGTTCAGCAAGCTTGTCTTTCTGGTGG
TGAAGGTTGTCTTTCTGTTGACCGAGAAGTTCCAGGGTATGTGGTCCGAAGTGAACGTGTAACCATTGATGCCTTTGACG
AAAATGGTACACCGCTTAAATTACGTTTCAAAGGTTATCCAGCGATTGTTATTCAACACGAAATCGATCATTTGAACGGT
ATTATGTTTTATGATCATATTAATAAAGAAAATCCATCATACTTGCCACCAGATGTGGATGTGTTTGGTTAA

Upstream 100 bases:

>100_bases
AAACACTCTTTTTTTCGAGTCTAGTAATCTTTCATTCCTTTTTTTTCGTTCTTTTGTTAAAATAAAGATAAGTTATTAAA
GAACAGGAGAGAATCAAATC

Downstream 100 bases:

>100_bases
ATTGAATAAATCCCCGCAGGAATTAAGATCCTGCGGGGATTTTTGACTTTATGAGTGACCACCAACTGTTTCTATTTTTG
TATCAAGCTGTCTGAATTTA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MLTMDDIVREGHPALREVATEVTFPLSDEEKKLGREMLEFLINSQDEELAEKYGLRGGVGIAAPQLAVTKRFLAIHVHDE
KDRLYSYVLYNPKIRSHSVQQACLSGGEGCLSVDREVPGYVVRSERVTIDAFDENGTPLKLRFKGYPAIVIQHEIDHLNG
IMFYDHINKENPSYLPPDVDVFG

Sequences:

>Translated_183_residues
MLTMDDIVREGHPALREVATEVTFPLSDEEKKLGREMLEFLINSQDEELAEKYGLRGGVGIAAPQLAVTKRFLAIHVHDE
KDRLYSYVLYNPKIRSHSVQQACLSGGEGCLSVDREVPGYVVRSERVTIDAFDENGTPLKLRFKGYPAIVIQHEIDHLNG
IMFYDHINKENPSYLPPDVDVFG
>Mature_183_residues
MLTMDDIVREGHPALREVATEVTFPLSDEEKKLGREMLEFLINSQDEELAEKYGLRGGVGIAAPQLAVTKRFLAIHVHDE
KDRLYSYVLYNPKIRSHSVQQACLSGGEGCLSVDREVPGYVVRSERVTIDAFDENGTPLKLRFKGYPAIVIQHEIDHLNG
IMFYDHINKENPSYLPPDVDVFG

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=111, Percent_Identity=38.7387387387387, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 20672; Mature: 20672

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTMDDIVREGHPALREVATEVTFPLSDEEKKLGREMLEFLINSQDEELAEKYGLRGGVG
CCCHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCC
IAAPQLAVTKRFLAIHVHDEKDRLYSYVLYNPKIRSHSVQQACLSGGEGCLSVDREVPGY
CCCCHHHHEEEEEEEEEECCHHHEEEEEEECCCCCCHHHHHHHHCCCCCEEEECCCCCCE
VVRSERVTIDAFDENGTPLKLRFKGYPAIVIQHEIDHLNGIMFYDHINKENPSYLPPDVD
EEECCEEEEEEECCCCCEEEEEECCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCC
VFG
CCC
>Mature Secondary Structure
MLTMDDIVREGHPALREVATEVTFPLSDEEKKLGREMLEFLINSQDEELAEKYGLRGGVG
CCCHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCC
IAAPQLAVTKRFLAIHVHDEKDRLYSYVLYNPKIRSHSVQQACLSGGEGCLSVDREVPGY
CCCCHHHHEEEEEEEEEECCHHHEEEEEEECCCCCCHHHHHHHHCCCCCEEEECCCCCCE
VVRSERVTIDAFDENGTPLKLRFKGYPAIVIQHEIDHLNGIMFYDHINKENPSYLPPDVD
EEECCEEEEEEECCCCCEEEEEECCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCC
VFG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]