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The map label for this gene is nagB [H]

Identifier: 226223575

GI number: 226223575

Start: 991301

End: 992005

Strand: Direct

Name: nagB [H]

Synonym: Lm4b_00977

Alternate gene names: 226223575

Gene position: 991301-992005 (Clockwise)

Preceding gene: 226223574

Following gene: 226223576

Centisome position: 34.03

GC content: 36.74

Gene sequence:

>705_bases
ATGCAACTTATCACAACAGAAAACAAATTAGCAGGATCCAAAAAAGCCTTAGAAATCATTGAAAAAGGCATTACATCAGG
TGAAGTTAACACACTTGGTCTAGCAACTGGTAGCACACCAGAAACACTTTATGCAGAGCTTGTAAAAAGCGATGTTGACA
CAAAAAATGTCACAACAACGAACTTAGATGAATATGTAGGTCTTGCGGCAAACGATCCAAACAGTTATCATTACTATATG
AATGAATTGTTATTCTCTAAAAAAGCATTTAAAGAAAGTTTCTTACCTAACGGAGAGGCGACGGATGCAGAAGCAGAATG
CGCACGTTATGAAGAAATTTTATCTGAGCACCCAATTGATATTCAAGTGCTTGGAATTGGAACAAACGGCCATATTGGTT
TTAACGAACCAGGAACTCCTTTTGATTCGTTAACACATAAAGTTGTTTTAACAGATTCCACTCGTGAAGCTAATAAACGC
TTTTTCGAAAGAGAAGAAGATGTTCCAACACATGCTTATTCTATGGGAATTAAATCCATCATGAATGCGAAGAAAATTAT
CCTACTTGCTTTTGGTGAAAACAAAGCACAAGCAATCAAAGAAACTATTAAAGGACCTGTAGATGTGAATTGTCCTGCTT
CTGTACTTCAAAATCATCCAGATGTTACTGTGATTCTTGACAACGAGGCGGCGTCACTTCTATAA

Upstream 100 bases:

>100_bases
GGCTGGTAAGGATGCAGATTTCAACTTACTTGACGAAGATTTACACATTACAGCAACTTATTCATTCGGAAAAAAACATT
CTTGAGAGGAAGATAATAAA

Downstream 100 bases:

>100_bases
GAAGAGGGCGGGAAAATGATCGATAAACAATCAGGAATACCGATTTACATTCAGATTCAAAGTGAAATTAAAAAGAAAAT
GGAAGATGGTGTTTGGAAAG

Product: glucosamine-6-phosphate isomerase

Products: NA

Alternate protein names: GlcN6P deaminase; GNPDA; Glucosamine-6-phosphate isomerase [H]

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MQLITTENKLAGSKKALEIIEKGITSGEVNTLGLATGSTPETLYAELVKSDVDTKNVTTTNLDEYVGLAANDPNSYHYYM
NELLFSKKAFKESFLPNGEATDAEAECARYEEILSEHPIDIQVLGIGTNGHIGFNEPGTPFDSLTHKVVLTDSTREANKR
FFEREEDVPTHAYSMGIKSIMNAKKIILLAFGENKAQAIKETIKGPVDVNCPASVLQNHPDVTVILDNEAASLL

Sequences:

>Translated_234_residues
MQLITTENKLAGSKKALEIIEKGITSGEVNTLGLATGSTPETLYAELVKSDVDTKNVTTTNLDEYVGLAANDPNSYHYYM
NELLFSKKAFKESFLPNGEATDAEAECARYEEILSEHPIDIQVLGIGTNGHIGFNEPGTPFDSLTHKVVLTDSTREANKR
FFEREEDVPTHAYSMGIKSIMNAKKIILLAFGENKAQAIKETIKGPVDVNCPASVLQNHPDVTVILDNEAASLL
>Mature_234_residues
MQLITTENKLAGSKKALEIIEKGITSGEVNTLGLATGSTPETLYAELVKSDVDTKNVTTTNLDEYVGLAANDPNSYHYYM
NELLFSKKAFKESFLPNGEATDAEAECARYEEILSEHPIDIQVLGIGTNGHIGFNEPGTPFDSLTHKVVLTDSTREANKR
FFEREEDVPTHAYSMGIKSIMNAKKIILLAFGENKAQAIKETIKGPVDVNCPASVLQNHPDVTVILDNEAASLL

Specific function: Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion [H]

COG id: COG0363

COG function: function code G; 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily [H]

Homologues:

Organism=Homo sapiens, GI13027378, Length=243, Percent_Identity=36.2139917695473, Blast_Score=132, Evalue=2e-31,
Organism=Homo sapiens, GI19923881, Length=243, Percent_Identity=34.9794238683128, Blast_Score=127, Evalue=7e-30,
Organism=Escherichia coli, GI1786893, Length=205, Percent_Identity=38.5365853658537, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1789530, Length=227, Percent_Identity=29.0748898678414, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI48994958, Length=231, Percent_Identity=29.4372294372294, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17554876, Length=242, Percent_Identity=34.7107438016529, Blast_Score=130, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24581960, Length=243, Percent_Identity=34.5679012345679, Blast_Score=133, Evalue=8e-32,
Organism=Drosophila melanogaster, GI19920764, Length=243, Percent_Identity=34.5679012345679, Blast_Score=133, Evalue=8e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006148
- InterPro:   IPR004547
- InterPro:   IPR018321 [H]

Pfam domain/function: PF01182 Glucosamine_iso [H]

EC number: =3.5.99.6 [H]

Molecular weight: Translated: 25562; Mature: 25562

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS01161 GLC_GALNAC_ISOMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLITTENKLAGSKKALEIIEKGITSGEVNTLGLATGSTPETLYAELVKSDVDTKNVTTT
CCEECCCHHHCCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCEEC
NLDEYVGLAANDPNSYHYYMNELLFSKKAFKESFLPNGEATDAEAECARYEEILSEHPID
CHHHHCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCE
IQVLGIGTNGHIGFNEPGTPFDSLTHKVVLTDSTREANKRFFEREEDVPTHAYSMGIKSI
EEEEEECCCCCCCCCCCCCCHHHHCEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHH
MNAKKIILLAFGENKAQAIKETIKGPVDVNCPASVLQNHPDVTVILDNEAASLL
HCCCEEEEEEECCCHHHHHHHHHCCCCCCCCCHHHHCCCCCEEEEECCCHHHCC
>Mature Secondary Structure
MQLITTENKLAGSKKALEIIEKGITSGEVNTLGLATGSTPETLYAELVKSDVDTKNVTTT
CCEECCCHHHCCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCEEC
NLDEYVGLAANDPNSYHYYMNELLFSKKAFKESFLPNGEATDAEAECARYEEILSEHPID
CHHHHCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCE
IQVLGIGTNGHIGFNEPGTPFDSLTHKVVLTDSTREANKRFFEREEDVPTHAYSMGIKSI
EEEEEECCCCCCCCCCCCCCHHHHCEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHH
MNAKKIILLAFGENKAQAIKETIKGPVDVNCPASVLQNHPDVTVILDNEAASLL
HCCCEEEEEEECCCHHHHHHHHHCCCCCCCCCHHHHCCCCCEEEEECCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]