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The map label for this gene is nagB [H]

Identifier: 226223496

GI number: 226223496

Start: 916790

End: 917518

Strand: Direct

Name: nagB [H]

Synonym: Lm4b_00895

Alternate gene names: 226223496

Gene position: 916790-917518 (Clockwise)

Preceding gene: 226223495

Following gene: 226223497

Centisome position: 31.48

GC content: 40.05

Gene sequence:

>729_bases
ATGAAATTAATTATTAATGAAAATGAACAACTCGTGGCGCAAACAGTGAGCCAAAGAATCATTGAATTGGTAAAAGAAAA
ACCTGCAAGTTTAATTTGTATCGCTGGTGGGGACACGCCTTTACTAACAATAGAAGCGCTTATTAAAGCGAATCAAACGG
GCGAAGTAGATTTTAGTGAGACGCAATTTGTTGGTTTGGATGAATGGGTTGGCCTTGGACGCGAAACAAAAGGTAGCTGT
ATCCAAACACTCTATGATGCATTTTTCGACCGTTTGAAAAATGTTTCTAGTGAACAAATTTGCTTTTTCGATGGGAAAGC
AACGAGCTTAACGGATGAATGCGCTCGTGTAGACAAGTTTATCGACGACCGTGGCGGAATGGATTTTATTTTGCTGGGGA
TTGGACTTAATGGGCATATTGGCTTCAACGAGCCATTTGTGCCAGTAGATGTGAACTGTCATGTCGTGGAACTTGATGAT
GTAACGAAACGCGTCATGAGCAAATATTTTGATACAGATTTGCCACTAACACACGGAATTTCTCTTGGTATGCAACAAAT
TTTAGCGGCGAAAGAAATATATTTAGTAGCAACTGGCGAGAAAAAAATTGATATCGTGAAACAAGTAGTGGAAAAAGAAC
CGACAGTAGCGATTCCTGCAACACTCGTAAAAGATTCGAATACAACACTTGTAGTCGATAAAATTGCCGCGAGTGGGGTG
GAAGCATAA

Upstream 100 bases:

>100_bases
ATGAGAAGAGCTTGATTAAAGAAGAAGAAGTTGTATCAGAACGCCAAGCTGCGCACGATGCACTAGACTTGGATTTCTAA
TAAGAAAGGTTGATTTTAAA

Downstream 100 bases:

>100_bases
TGGAAAAAATTATCGTTAACGGCAGAAAAAATGGACAAAAAATCGAGATAGCTTGCTCTAATTTAGTGATGGGTGCAGGA
GATTTCTTACGTGCAGATAA

Product: NagB protein (glucosamine-6-phosphate isomerase)

Products: NA

Alternate protein names: GlcN6P deaminase; GNPDA; Glucosamine-6-phosphate isomerase [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MKLIINENEQLVAQTVSQRIIELVKEKPASLICIAGGDTPLLTIEALIKANQTGEVDFSETQFVGLDEWVGLGRETKGSC
IQTLYDAFFDRLKNVSSEQICFFDGKATSLTDECARVDKFIDDRGGMDFILLGIGLNGHIGFNEPFVPVDVNCHVVELDD
VTKRVMSKYFDTDLPLTHGISLGMQQILAAKEIYLVATGEKKIDIVKQVVEKEPTVAIPATLVKDSNTTLVVDKIAASGV
EA

Sequences:

>Translated_242_residues
MKLIINENEQLVAQTVSQRIIELVKEKPASLICIAGGDTPLLTIEALIKANQTGEVDFSETQFVGLDEWVGLGRETKGSC
IQTLYDAFFDRLKNVSSEQICFFDGKATSLTDECARVDKFIDDRGGMDFILLGIGLNGHIGFNEPFVPVDVNCHVVELDD
VTKRVMSKYFDTDLPLTHGISLGMQQILAAKEIYLVATGEKKIDIVKQVVEKEPTVAIPATLVKDSNTTLVVDKIAASGV
EA
>Mature_242_residues
MKLIINENEQLVAQTVSQRIIELVKEKPASLICIAGGDTPLLTIEALIKANQTGEVDFSETQFVGLDEWVGLGRETKGSC
IQTLYDAFFDRLKNVSSEQICFFDGKATSLTDECARVDKFIDDRGGMDFILLGIGLNGHIGFNEPFVPVDVNCHVVELDD
VTKRVMSKYFDTDLPLTHGISLGMQQILAAKEIYLVATGEKKIDIVKQVVEKEPTVAIPATLVKDSNTTLVVDKIAASGV
EA

Specific function: Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion [H]

COG id: COG0363

COG function: function code G; 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily [H]

Homologues:

Organism=Homo sapiens, GI19923881, Length=219, Percent_Identity=27.8538812785388, Blast_Score=94, Evalue=8e-20,
Organism=Homo sapiens, GI13027378, Length=221, Percent_Identity=29.4117647058824, Blast_Score=89, Evalue=2e-18,
Organism=Escherichia coli, GI1789530, Length=217, Percent_Identity=34.5622119815668, Blast_Score=137, Evalue=5e-34,
Organism=Escherichia coli, GI1786893, Length=218, Percent_Identity=26.1467889908257, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI48994958, Length=241, Percent_Identity=24.4813278008299, Blast_Score=61, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17554876, Length=217, Percent_Identity=28.110599078341, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24581960, Length=244, Percent_Identity=25, Blast_Score=97, Evalue=9e-21,
Organism=Drosophila melanogaster, GI19920764, Length=244, Percent_Identity=25, Blast_Score=97, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006148
- InterPro:   IPR004547
- InterPro:   IPR018321 [H]

Pfam domain/function: PF01182 Glucosamine_iso [H]

EC number: =3.5.99.6 [H]

Molecular weight: Translated: 26465; Mature: 26465

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIINENEQLVAQTVSQRIIELVKEKPASLICIAGGDTPLLTIEALIKANQTGEVDFSE
CEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCC
TQFVGLDEWVGLGRETKGSCIQTLYDAFFDRLKNVSSEQICFFDGKATSLTDECARVDKF
CEEECCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHH
IDDRGGMDFILLGIGLNGHIGFNEPFVPVDVNCHVVELDDVTKRVMSKYFDTDLPLTHGI
HHCCCCCEEEEEEECCCCCCCCCCCEEEEECCEEEEEHHHHHHHHHHHHCCCCCCHHHHH
SLGMQQILAAKEIYLVATGEKKIDIVKQVVEKEPTVAIPATLVKDSNTTLVVDKIAASGV
HHHHHHHHHHCEEEEEEECCHHHHHHHHHHHCCCCEEECCEEEECCCCEEEEEEHHHCCC
EA
CC
>Mature Secondary Structure
MKLIINENEQLVAQTVSQRIIELVKEKPASLICIAGGDTPLLTIEALIKANQTGEVDFSE
CEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCC
TQFVGLDEWVGLGRETKGSCIQTLYDAFFDRLKNVSSEQICFFDGKATSLTDECARVDKF
CEEECCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHH
IDDRGGMDFILLGIGLNGHIGFNEPFVPVDVNCHVVELDDVTKRVMSKYFDTDLPLTHGI
HHCCCCCEEEEEEECCCCCCCCCCCEEEEECCEEEEEHHHHHHHHHHHHCCCCCCHHHHH
SLGMQQILAAKEIYLVATGEKKIDIVKQVVEKEPTVAIPATLVKDSNTTLVVDKIAASGV
HHHHHHHHHHCEEEEEEECCHHHHHHHHHHHCCCCEEECCEEEECCCCEEEEEEHHHCCC
EA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA