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The map label for this gene is lplA [H]

Identifier: 226223382

GI number: 226223382

Start: 789346

End: 790335

Strand: Direct

Name: lplA [H]

Synonym: Lm4b_00780

Alternate gene names: 226223382

Gene position: 789346-790335 (Clockwise)

Preceding gene: 226223381

Following gene: 226223383

Centisome position: 27.1

GC content: 37.88

Gene sequence:

>990_bases
GTGATTTATTTAGATAATGAAGATGTACTCGATCAAGCGTACAACTTTGCGATGGAAGAATATGCGCTTCGTTCTTTAGA
TGAAAATGAAACGTATTTTATGTTTTACCGGATGAAGCCGACGATTATTGTTGGTAAAAATCAAAACACGCTGGAAGAAA
TCAACCATGCTTTTGTCAAAGAACATCATATCGACGTTCTCCGCCGTTTATCTGGTGGGGGAGCTGTCTATAACGACGAA
GGCAATATTAGCTTTAGCATGATCACAAAAGATGATGGGAATAGTTTTCAAAATTTCGCGAAGTTCACAGAACCAGTGAT
TCGAGCGCTCCGCAAGCTTGGTGTAAATGCTGAACTGAGTGGTCGAAATGATATTGAAGTGAATGGCAAAAAGATAAGTG
GCAATGCGCAATTTGCAACAAAAGGTCGACTATATAGCCATGGGACTTTACTTTTCGATGTCGATTTATCCATGCTCGAA
AAAGCATTACAAGTGGATCCAGAGAAATACTTATCCAAGGGTGTTAAATCTGTGCGCAGTCGTGTAACAACAATTCGCGA
ACATTTAGCAGAAGATATCGATATTCTAACCTTTAAACAAATTCTACTGGAATCTATTTTTGAAACGAAAGATATCCCAC
GTTATACTTTTACGGAAGCTGACAAGCAAGGAATCGAAAAATTACGCACAGAACGTTACCGGAATTGGGACTGGACGTAT
GGTAAATCACCAAAAGCCACTATAAAACGCAAAAAAAGATTTCCAGCTGGAACTATTGAATTCCAAGTTTCACTAGAAAA
AGGTCAAGTAAAAGAAGCGACCATTTATGGGGACTTTTTTGGTACAGAGGATGTGGCTGAATTAGCTGGGAAAATAATCG
GCTGCCGTTTTGAACGTAAATCTATTCAAAATGCTTGGCAAGAAATAAATGCGAAAGACTATTTTGGTGGAATTGAAAAA
GAAGCGATTTTAGATATGTTATTTGAATAA

Upstream 100 bases:

>100_bases
TGAGCCGCGGCTTAGGAAATGTCACGAAACTACCGCGCTTATTTAATGACCCAGAAATCATTCGAATGACACTTAGAGCA
AAAGGGGATGCATAGGAAAA

Downstream 100 bases:

>100_bases
AGTTTAAAACTCTGAGATATAGATTCTCAGAGTTTTTTTTAATGCCTAAAGAAAGCGATTTCAATTTTATGTTGACAGAG
ATTAAATATATATAGTATTA

Product: lipoate-protein ligase

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MIYLDNEDVLDQAYNFAMEEYALRSLDENETYFMFYRMKPTIIVGKNQNTLEEINHAFVKEHHIDVLRRLSGGGAVYNDE
GNISFSMITKDDGNSFQNFAKFTEPVIRALRKLGVNAELSGRNDIEVNGKKISGNAQFATKGRLYSHGTLLFDVDLSMLE
KALQVDPEKYLSKGVKSVRSRVTTIREHLAEDIDILTFKQILLESIFETKDIPRYTFTEADKQGIEKLRTERYRNWDWTY
GKSPKATIKRKKRFPAGTIEFQVSLEKGQVKEATIYGDFFGTEDVAELAGKIIGCRFERKSIQNAWQEINAKDYFGGIEK
EAILDMLFE

Sequences:

>Translated_329_residues
MIYLDNEDVLDQAYNFAMEEYALRSLDENETYFMFYRMKPTIIVGKNQNTLEEINHAFVKEHHIDVLRRLSGGGAVYNDE
GNISFSMITKDDGNSFQNFAKFTEPVIRALRKLGVNAELSGRNDIEVNGKKISGNAQFATKGRLYSHGTLLFDVDLSMLE
KALQVDPEKYLSKGVKSVRSRVTTIREHLAEDIDILTFKQILLESIFETKDIPRYTFTEADKQGIEKLRTERYRNWDWTY
GKSPKATIKRKKRFPAGTIEFQVSLEKGQVKEATIYGDFFGTEDVAELAGKIIGCRFERKSIQNAWQEINAKDYFGGIEK
EAILDMLFE
>Mature_329_residues
MIYLDNEDVLDQAYNFAMEEYALRSLDENETYFMFYRMKPTIIVGKNQNTLEEINHAFVKEHHIDVLRRLSGGGAVYNDE
GNISFSMITKDDGNSFQNFAKFTEPVIRALRKLGVNAELSGRNDIEVNGKKISGNAQFATKGRLYSHGTLLFDVDLSMLE
KALQVDPEKYLSKGVKSVRSRVTTIREHLAEDIDILTFKQILLESIFETKDIPRYTFTEADKQGIEKLRTERYRNWDWTY
GKSPKATIKRKKRFPAGTIEFQVSLEKGQVKEATIYGDFFGTEDVAELAGKIIGCRFERKSIQNAWQEINAKDYFGGIEK
EAILDMLFE

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=252, Percent_Identity=27.3809523809524, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI21729884, Length=252, Percent_Identity=27.3809523809524, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI21729882, Length=252, Percent_Identity=27.3809523809524, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI21729880, Length=252, Percent_Identity=27.3809523809524, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI21729878, Length=252, Percent_Identity=27.3809523809524, Blast_Score=88, Evalue=9e-18,
Organism=Escherichia coli, GI1790846, Length=326, Percent_Identity=32.2085889570552, Blast_Score=162, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI71981909, Length=249, Percent_Identity=28.1124497991968, Blast_Score=103, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6322415, Length=284, Percent_Identity=27.8169014084507, Blast_Score=96, Evalue=7e-21,
Organism=Drosophila melanogaster, GI28573464, Length=286, Percent_Identity=29.3706293706294, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 37846; Mature: 37846

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYLDNEDVLDQAYNFAMEEYALRSLDENETYFMFYRMKPTIIVGKNQNTLEEINHAFVK
CEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCEEEEECCCCHHHHHHHHHHH
EHHIDVLRRLSGGGAVYNDEGNISFSMITKDDGNSFQNFAKFTEPVIRALRKLGVNAELS
HHHHHHHHHHCCCCEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEC
GRNDIEVNGKKISGNAQFATKGRLYSHGTLLFDVDLSMLEKALQVDPEKYLSKGVKSVRS
CCCCEEECCEEECCCCEEEECCEEEECCEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHH
RVTTIREHLAEDIDILTFKQILLESIFETKDIPRYTFTEADKQGIEKLRTERYRNWDWTY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
GKSPKATIKRKKRFPAGTIEFQVSLEKGQVKEATIYGDFFGTEDVAELAGKIIGCRFERK
CCCCHHHHHHHHCCCCCEEEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHCCCHHHH
SIQNAWQEINAKDYFGGIEKEAILDMLFE
HHHHHHHHCCCHHHCCCCCHHHHHHHHCC
>Mature Secondary Structure
MIYLDNEDVLDQAYNFAMEEYALRSLDENETYFMFYRMKPTIIVGKNQNTLEEINHAFVK
CEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCEEEEECCCCHHHHHHHHHHH
EHHIDVLRRLSGGGAVYNDEGNISFSMITKDDGNSFQNFAKFTEPVIRALRKLGVNAELS
HHHHHHHHHHCCCCEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEC
GRNDIEVNGKKISGNAQFATKGRLYSHGTLLFDVDLSMLEKALQVDPEKYLSKGVKSVRS
CCCCEEECCEEECCCCEEEECCEEEECCEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHH
RVTTIREHLAEDIDILTFKQILLESIFETKDIPRYTFTEADKQGIEKLRTERYRNWDWTY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
GKSPKATIKRKKRFPAGTIEFQVSLEKGQVKEATIYGDFFGTEDVAELAGKIIGCRFERK
CCCCHHHHHHHHCCCCCEEEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHCCCHHHH
SIQNAWQEINAKDYFGGIEKEAILDMLFE
HHHHHHHHCCCHHHCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]