Click here to switch to the map view.

The map label for this gene is fliM

Identifier: 226223330

GI number: 226223330

Start: 740506

End: 741498

Strand: Direct

Name: fliM

Synonym: Lm4b_00727

Alternate gene names: 226223330

Gene position: 740506-741498 (Clockwise)

Preceding gene: 226223329

Following gene: 226223331

Centisome position: 25.42

GC content: 41.09

Gene sequence:

>993_bases
ATGAGCGATAAATTAAGTCAAGAACAAATTGACGCCCTGCTTTCCCAAATAAGTGAAGGTAAGGTAGTTGATGAAAGTAC
GGAAATTGGCGACTTTGGGCGCTTTCATCCCTATGACTTTCATAAACCAGAGAAATTTGGCGCAGAACACCTCGAAAGCT
TAAAGACGATTGCATCCGCTTTTACGAAAAAAAGCATGGAGTTTGTTTCCCAGCGTATCCGGATTCCAATTCATACCGAA
GCAACCCTTGCAGATCAAGTTTCCTTTGCGAGTGGTTATATCGAAACAATGCCGAACGATAGCTACATTTTCTGCATTAT
CGACCTGGGTAATCCGGAGCTCGGTCAAATTATTATCGAACTCGATTTAGCCTATATCATTTATATTCATGAATGCTTAT
CTGGCGGGAATCCAAAACGAAAATTGAGCGAGCGCAGACTTTTATCTGTTTTTGAAGAGCTGACGTTGAAATCTATTTTA
GAAAAATTCTGCGAAGCACTCAAAGACAGTTTTAAATCAGTGCATCCAATCTCACCAGAAATCGTTAATATCGAAACTAA
CCCAGCACTTCTACGTGTCACATCACCGAACGACATGATGGCGCTTGTCAGTGTTGATATTAAATCAGAGTTTTGGATTA
GTACAATGCGCATTGGCGTGCCGTTTTTCTCGGTGGAAGAGATTATGAATAAGCTGGAAAACGTTGTGGAATATACGTTT
GATAAACGGCGGAACTTTGATGCGGAAGTCGAGCAAGAATTGCATCAAGTTGAGAAAGAAGCGCGCATACGTGTAGGTGA
AATTAAAACAACGTGGAAAGAACTGAATAAGCTTGAAGTAGGCGACGTGCTTCTGACAGAAACTCATATACGTGACACAC
TCAAAGGCTACGTCACTGAAAAATGGAAATTTGAATGTTATATGGGAAAAAGTGGTAACCAAAAAGCCGTTAAATTTATG
CGTCATACAGGGCGGACAGAGCAGGAGAGGTAG

Upstream 100 bases:

>100_bases
TCGGTTACGGTGAGATTTTGACAAAAGACGGCAAGATGTTTGTCAAAATAACGAAATTAGGAGAGGGAAGTTCTTCATGA
GAGCTAGGGAGTGACAAAAA

Downstream 100 bases:

>100_bases
AAGTGGAGCAATTACTAGAGAAAAATATCACGCAAATGGAACTAGATGTGATAGGAGAAATCGCCAATATTTCGTTTGGC
TCAGCTTCTACGGTCTTATC

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MSDKLSQEQIDALLSQISEGKVVDESTEIGDFGRFHPYDFHKPEKFGAEHLESLKTIASAFTKKSMEFVSQRIRIPIHTE
ATLADQVSFASGYIETMPNDSYIFCIIDLGNPELGQIIIELDLAYIIYIHECLSGGNPKRKLSERRLLSVFEELTLKSIL
EKFCEALKDSFKSVHPISPEIVNIETNPALLRVTSPNDMMALVSVDIKSEFWISTMRIGVPFFSVEEIMNKLENVVEYTF
DKRRNFDAEVEQELHQVEKEARIRVGEIKTTWKELNKLEVGDVLLTETHIRDTLKGYVTEKWKFECYMGKSGNQKAVKFM
RHTGRTEQER

Sequences:

>Translated_330_residues
MSDKLSQEQIDALLSQISEGKVVDESTEIGDFGRFHPYDFHKPEKFGAEHLESLKTIASAFTKKSMEFVSQRIRIPIHTE
ATLADQVSFASGYIETMPNDSYIFCIIDLGNPELGQIIIELDLAYIIYIHECLSGGNPKRKLSERRLLSVFEELTLKSIL
EKFCEALKDSFKSVHPISPEIVNIETNPALLRVTSPNDMMALVSVDIKSEFWISTMRIGVPFFSVEEIMNKLENVVEYTF
DKRRNFDAEVEQELHQVEKEARIRVGEIKTTWKELNKLEVGDVLLTETHIRDTLKGYVTEKWKFECYMGKSGNQKAVKFM
RHTGRTEQER
>Mature_329_residues
SDKLSQEQIDALLSQISEGKVVDESTEIGDFGRFHPYDFHKPEKFGAEHLESLKTIASAFTKKSMEFVSQRIRIPIHTEA
TLADQVSFASGYIETMPNDSYIFCIIDLGNPELGQIIIELDLAYIIYIHECLSGGNPKRKLSERRLLSVFEELTLKSILE
KFCEALKDSFKSVHPISPEIVNIETNPALLRVTSPNDMMALVSVDIKSEFWISTMRIGVPFFSVEEIMNKLENVVEYTFD
KRRNFDAEVEQELHQVEKEARIRVGEIKTTWKELNKLEVGDVLLTETHIRDTLKGYVTEKWKFECYMGKSGNQKAVKFMR
HTGRTEQER

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Potential). Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

None

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 37968; Mature: 37837

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKLSQEQIDALLSQISEGKVVDESTEIGDFGRFHPYDFHKPEKFGAEHLESLKTIASA
CCCCCCHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
FTKKSMEFVSQRIRIPIHTEATLADQVSFASGYIETMPNDSYIFCIIDLGNPELGQIIIE
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHCCCCCCEEEEEEECCCCCHHHHHHH
LDLAYIIYIHECLSGGNPKRKLSERRLLSVFEELTLKSILEKFCEALKDSFKSVHPISPE
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
IVNIETNPALLRVTSPNDMMALVSVDIKSEFWISTMRIGVPFFSVEEIMNKLENVVEYTF
EEEEECCCEEEEEECCCCEEEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
DKRRNFDAEVEQELHQVEKEARIRVGEIKTTWKELNKLEVGDVLLTETHIRDTLKGYVTE
HHHCCCCHHHHHHHHHHHHHHCEEHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHCC
KWKFECYMGKSGNQKAVKFMRHTGRTEQER
CEEEEEEECCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SDKLSQEQIDALLSQISEGKVVDESTEIGDFGRFHPYDFHKPEKFGAEHLESLKTIASA
CCCCCHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
FTKKSMEFVSQRIRIPIHTEATLADQVSFASGYIETMPNDSYIFCIIDLGNPELGQIIIE
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHCCCCCCEEEEEEECCCCCHHHHHHH
LDLAYIIYIHECLSGGNPKRKLSERRLLSVFEELTLKSILEKFCEALKDSFKSVHPISPE
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
IVNIETNPALLRVTSPNDMMALVSVDIKSEFWISTMRIGVPFFSVEEIMNKLENVVEYTF
EEEEECCCEEEEEECCCCEEEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
DKRRNFDAEVEQELHQVEKEARIRVGEIKTTWKELNKLEVGDVLLTETHIRDTLKGYVTE
HHHCCCCHHHHHHHHHHHHHHCEEHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHCC
KWKFECYMGKSGNQKAVKFMRHTGRTEQER
CEEEEEEECCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8755894; 9665876; 8529894 [H]