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The map label for this gene is hisF

Identifier: 226223192

GI number: 226223192

Start: 605109

End: 605864

Strand: Reverse

Name: hisF

Synonym: Lm4b_00589

Alternate gene names: 226223192

Gene position: 605864-605109 (Counterclockwise)

Preceding gene: 226223193

Following gene: 226223191

Centisome position: 20.8

GC content: 44.97

Gene sequence:

>756_bases
TTGCTTACTAAAAGAATCATCCCATGTCTTGATGTCACAGCAGGTCGAGTCGTCAAAGGTGTTAATTTTGTTTCCTTAAC
AGATGTCGGGGACCCTGTCGAAATTGCTAAAGCTTATAATGAAGCTGGTGCCGATGAGCTCGTCTTTCTTGATATCACGG
CAACTGTCGAACTCCGTCAAACCATGATAGATGTTGTGGAACGAACTGCTGAGCAAGTTTTTATTCCACTCACAGTTGGT
GGGGGTATCAGTAGTGTTTCTGACATGAAAGAACTTCTTCAAGCCGGAGCCGACAAGATTTCGCTTAATTCCGCCGCCAT
CAAACGACCAGAACTCATCCAAGAAGGAGCAGCAAAATTCGGAAACCAATGTATCGTTGTCGCAATTGATGCAAAATGGA
ATGGTACAAATTGGAGCGTCTTCACGAGAGGCGGTCGAAATGATACCGGACTTGATGCTATTGAATGGGCTAAAAAAGCT
GTACAACTTGGTGCTGGTGAAATCCTACTAACAAGTATGGACGGCGATGGAACCAAAAACGGTTACGATATTCCGCTCAC
AAAAGCAATTTCAGCAGCAGTTTCCGTCCCAGTCATTGCTTCAGGCGGTTGCGGGAATGCCGCACATATGGCCGAGGTTT
TTGAAAAAACAAAGGCAACCGCCGCTCTCGCTGCAAGCATTTTCCACTACGGTGAACTAAGCATCAAAAATGTCAAAACA
ACCTTACTCGAAAAAGGAGTGAATATTCGTCCATGA

Upstream 100 bases:

>100_bases
TTGGAAGCACTCGCCCAACTCGGCTTATATGGAGCAATTGCCGGAAAAGCCCTTTATAATCATGATATTTCCATGTCAGA
TATTGTAGAGGTGGAACAAA

Downstream 100 bases:

>100_bases
CTTCTGTAGATTTTTCTAAAGGTCTTGTCCCAACTATTATCCTAGACGATCAAAATGGCGACGTTTTAATGCTCGCTTAT
ATGAACGAAGAAAGTTATCA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MLTKRIIPCLDVTAGRVVKGVNFVSLTDVGDPVEIAKAYNEAGADELVFLDITATVELRQTMIDVVERTAEQVFIPLTVG
GGISSVSDMKELLQAGADKISLNSAAIKRPELIQEGAAKFGNQCIVVAIDAKWNGTNWSVFTRGGRNDTGLDAIEWAKKA
VQLGAGEILLTSMDGDGTKNGYDIPLTKAISAAVSVPVIASGGCGNAAHMAEVFEKTKATAALAASIFHYGELSIKNVKT
TLLEKGVNIRP

Sequences:

>Translated_251_residues
MLTKRIIPCLDVTAGRVVKGVNFVSLTDVGDPVEIAKAYNEAGADELVFLDITATVELRQTMIDVVERTAEQVFIPLTVG
GGISSVSDMKELLQAGADKISLNSAAIKRPELIQEGAAKFGNQCIVVAIDAKWNGTNWSVFTRGGRNDTGLDAIEWAKKA
VQLGAGEILLTSMDGDGTKNGYDIPLTKAISAAVSVPVIASGGCGNAAHMAEVFEKTKATAALAASIFHYGELSIKNVKT
TLLEKGVNIRP
>Mature_251_residues
MLTKRIIPCLDVTAGRVVKGVNFVSLTDVGDPVEIAKAYNEAGADELVFLDITATVELRQTMIDVVERTAEQVFIPLTVG
GGISSVSDMKELLQAGADKISLNSAAIKRPELIQEGAAKFGNQCIVVAIDAKWNGTNWSVFTRGGRNDTGLDAIEWAKKA
VQLGAGEILLTSMDGDGTKNGYDIPLTKAISAAVSVPVIASGGCGNAAHMAEVFEKTKATAALAASIFHYGELSIKNVKT
TLLEKGVNIRP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=256, Percent_Identity=44.53125, Blast_Score=203, Evalue=7e-54,
Organism=Escherichia coli, GI87082028, Length=206, Percent_Identity=27.6699029126214, Blast_Score=78, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6319725, Length=313, Percent_Identity=34.8242811501597, Blast_Score=161, Evalue=9e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 26447; Mature: 26447

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTKRIIPCLDVTAGRVVKGVNFVSLTDVGDPVEIAKAYNEAGADELVFLDITATVELRQ
CCCCCCCCHHCCCHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCCCEEEEEEEHHHHHHH
TMIDVVERTAEQVFIPLTVGGGISSVSDMKELLQAGADKISLNSAAIKRPELIQEGAAKF
HHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCCEEECCHHHCCCHHHHHHHHHHH
GNQCIVVAIDAKWNGTNWSVFTRGGRNDTGLDAIEWAKKAVQLGAGEILLTSMDGDGTKN
CCCEEEEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCC
GYDIPLTKAISAAVSVPVIASGGCGNAAHMAEVFEKTKATAALAASIFHYGELSIKNVKT
CCCCCHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEHHHHHH
TLLEKGVNIRP
HHHHCCCCCCC
>Mature Secondary Structure
MLTKRIIPCLDVTAGRVVKGVNFVSLTDVGDPVEIAKAYNEAGADELVFLDITATVELRQ
CCCCCCCCHHCCCHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCCCEEEEEEEHHHHHHH
TMIDVVERTAEQVFIPLTVGGGISSVSDMKELLQAGADKISLNSAAIKRPELIQEGAAKF
HHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCCEEECCHHHCCCHHHHHHHHHHH
GNQCIVVAIDAKWNGTNWSVFTRGGRNDTGLDAIEWAKKAVQLGAGEILLTSMDGDGTKN
CCCEEEEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCC
GYDIPLTKAISAAVSVPVIASGGCGNAAHMAEVFEKTKATAALAASIFHYGELSIKNVKT
CCCCCHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEHHHHHH
TLLEKGVNIRP
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]