Click here to switch to the map view.

The map label for this gene is hisI

Identifier: 226223191

GI number: 226223191

Start: 604795

End: 605112

Strand: Reverse

Name: hisI

Synonym: Lm4b_00588

Alternate gene names: 226223191

Gene position: 605112-604795 (Counterclockwise)

Preceding gene: 226223192

Following gene: 226223190

Centisome position: 20.78

GC content: 39.31

Gene sequence:

>318_bases
ATGACTTCTGTAGATTTTTCTAAAGGTCTTGTCCCAACTATTATCCTAGACGATCAAAATGGCGACGTTTTAATGCTCGC
TTATATGAACGAAGAAAGTTATCAAAAAACCCTTGAAACTGGCTATACTTGGTTTTTTTCGCGCTCCAGAAACGAACTTT
GGAATAAAGGCGCGACAAGCGGACATACTCAAAAAGTAAAACAAATCTGGACCGATTGTGATAACGACACATTACTTATC
CGCGTAACCCAAATCGGTCCAGCCTGCCACACCGGCAAAAAAAGCTGCTTTTTCAATCTTATAAAGGAGGATTTTTAA

Upstream 100 bases:

>100_bases
AGGCAACCGCCGCTCTCGCTGCAAGCATTTTCCACTACGGTGAACTAAGCATCAAAAATGTCAAAACAACCTTACTCGAA
AAAGGAGTGAATATTCGTCC

Downstream 100 bases:

>100_bases
ATGTTAAATGACCTATATGAAGAAATTAAACTGCGTAAAACTCAACCAAGAGAAGGCTCCTATACCAACTATTTGTTCGA
TAAAGGCCTTGATAAAATTC

Product: phosphoribosyl-AMP cyclohydrolase

Products: NA

Alternate protein names: PRA-CH [H]

Number of amino acids: Translated: 105; Mature: 104

Protein sequence:

>105_residues
MTSVDFSKGLVPTIILDDQNGDVLMLAYMNEESYQKTLETGYTWFFSRSRNELWNKGATSGHTQKVKQIWTDCDNDTLLI
RVTQIGPACHTGKKSCFFNLIKEDF

Sequences:

>Translated_105_residues
MTSVDFSKGLVPTIILDDQNGDVLMLAYMNEESYQKTLETGYTWFFSRSRNELWNKGATSGHTQKVKQIWTDCDNDTLLI
RVTQIGPACHTGKKSCFFNLIKEDF
>Mature_104_residues
TSVDFSKGLVPTIILDDQNGDVLMLAYMNEESYQKTLETGYTWFFSRSRNELWNKGATSGHTQKVKQIWTDCDNDTLLIR
VTQIGPACHTGKKSCFFNLIKEDF

Specific function: Histidine biosynthesis; second step. Histidine biosynthesis; third step. [C]

COG id: COG0139

COG function: function code E; Phosphoribosyl-AMP cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PRA-CH family [H]

Homologues:

Organism=Escherichia coli, GI2367128, Length=89, Percent_Identity=53.9325842696629, Blast_Score=104, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6319819, Length=94, Percent_Identity=43.6170212765958, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002496
- ProDom:   PD002610 [H]

Pfam domain/function: PF01502 PRA-CH [H]

EC number: =3.5.4.19 [H]

Molecular weight: Translated: 12034; Mature: 11903

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSVDFSKGLVPTIILDDQNGDVLMLAYMNEESYQKTLETGYTWFFSRSRNELWNKGATS
CCCCCCCCCCCEEEEEECCCCCEEEEEEECCHHHHHHHHCCCEEEEECCCHHHHCCCCCC
GHTQKVKQIWTDCDNDTLLIRVTQIGPACHTGKKSCFFNLIKEDF
CHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TSVDFSKGLVPTIILDDQNGDVLMLAYMNEESYQKTLETGYTWFFSRSRNELWNKGATS
CCCCCCCCCCEEEEEECCCCCEEEEEEECCHHHHHHHHCCCEEEEECCCHHHHCCCCCC
GHTQKVKQIWTDCDNDTLLIRVTQIGPACHTGKKSCFFNLIKEDF
CHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]