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The map label for this gene is cocE [H]

Identifier: 226223119

GI number: 226223119

Start: 527022

End: 528782

Strand: Reverse

Name: cocE [H]

Synonym: Lm4b_00516

Alternate gene names: 226223119

Gene position: 528782-527022 (Counterclockwise)

Preceding gene: 226223137

Following gene: 226223118

Centisome position: 18.15

GC content: 38.5

Gene sequence:

>1761_bases
ATGACTGCAAAGATAACAGAAAAAATGCTTTTCAGCCCTTCTTTTGAATACGTGGATAATGGCATCGAGCATGGAGTGCT
AAGTCCATTCGAAACAAAAGAAATCATTTTAGAAAAAGGACAACAAATTGCTGAAGGTTTCAAACCACTGGATTGTTCCA
TTAAAATGTTAAAAGATGTACCAGTTAAGATGCGTGACGGTATAACCATTTATACCGATATTTATTTACCAATAACAGAA
GAAAAAGTTCCTACTCTTATCGCCTGGAGCCCTTATGGAAAAAGTGCTGGAACAGCTCCTCGATACAAAAATCTATTTAA
TATGCTAGGAATGGGGAATGCCTGGAATTCTGGCCTTACTAAATTTGAAGCACCTGATCCAGCATACTGGTGCGCACATG
GATACGCTGTCTGTAATCCTGACATGCGTGGCATTGCTCATAGTGAGGGTAATACTACAATGATTGGCTCTCAAGAAGCT
GAAGATGGCTATGATTTAATTGAATGGTTAGCTAAGCAATCTTGGAGTAATGGAAAAACAGCTCTAACAGGAACATCTTA
TTTAGCCTTTTCCCAGTGGTATATTGCAGCTGAGCAGCCCCCTCACCTAACATGTATCAATCCTACTGAAGGATTAGCAG
ATGGTTATCGTGACTTAGCTTTTATTGGAGGCATTCCAGATCCTAATTTTATTGAGCGTTTGCAAATCAACCATGTCAGT
GCTAAAAATTCGCAAAGAGAAGATTTAACAAAGGAAATGGAAGCATATCCTTTAGCTGATAGTGCTATTTGGAAAGACAA
AGTTGCAGATCCAAGTAAAATTACAATACCAGCTTTTGTTATTGCAAGTTATTCTAATACGCTTCACACAATGGGTACAT
TTCGTTCTTGGCGTTCACTTGGTTCTAAAGAAAAATGGCTTCGTATTCATGATCGCCAAGAATGGCCATATTACTATGAC
GAAAATAACGTAGAAGAATTGCGACGCTTTTTTGATTATTATTTACTTGGCAAGGAAAATAATTGGACAGATACACCAAC
TGTGCGCTATGCATTAATTGACTTTCAAGGTGGCAATCAAACTGACTTACCATCCGAAGTTTTTCCACCTACCAATAGTG
AAAACAAACGTTATTATATGAATGGCCTATTACGTACTTTACAAGAAACATCTACTTCAGATGATTTTAAAGTATCCTAT
ACTACTGAAAACTTGCCAGGGAGAGCATCGTTCCAAATGACGTTTGACAAAGAAACGCATTTTGTTGGTTATCCAAAAGC
AAAATTATTTATGGAAGTAGATGGATACGATGACTTGGATGTTTTTGTTTGGCTTCAAAAGCTAGATAAATTCGGCAACG
TACTAAGTGAATTTGTCGTGCCAAATCACGGGGCTGCACTTCAGGATTTCACACAAGAAGGGGCATCTGCTTTACGCTAT
AAAGGCGCTTGGGGAAGACTTCGCGCATCCATGCGACAACTAGATTCTCAAGTTTCAACAGATGAAATTCCTGCTTATAG
CTTTGACAAAGTAGAAAAACTCTCTTCCGGAGAAATTGTAGAACTAGACATTATTTTGAGTCCCATTGGTCTAACTTATA
AAGCAGGTGAAACACTCAGAGTAGTCGTGAGTAGCAAAGATGAACTTGGCTCAGTAATGCCAGGAACGCCCGGATGCACA
CCTAATAATAAAGGTATGCATATCCTTCATACGAGTAAAGAGCACCCATCCTATATTCAGCTACCAATAATCAACAAATA
G

Upstream 100 bases:

>100_bases
TAGGAGAGATTATCCAAAATTACAATTGTACAGACCTCGCGATACTACCTATACTTAAAAGTGTTAAAACTTATTAAAAA
AATAATCGGAGGTAATTTAT

Downstream 100 bases:

>100_bases
AAAAGCTTGTCAATGGAGTCTAATAACTTTATACTAAAGCTACTATTTACAATCTTTCATTGAATGGAGCAATTATGAAG
TTATCCAATTTGAAATACTT

Product: acylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 586; Mature: 585

Protein sequence:

>586_residues
MTAKITEKMLFSPSFEYVDNGIEHGVLSPFETKEIILEKGQQIAEGFKPLDCSIKMLKDVPVKMRDGITIYTDIYLPITE
EKVPTLIAWSPYGKSAGTAPRYKNLFNMLGMGNAWNSGLTKFEAPDPAYWCAHGYAVCNPDMRGIAHSEGNTTMIGSQEA
EDGYDLIEWLAKQSWSNGKTALTGTSYLAFSQWYIAAEQPPHLTCINPTEGLADGYRDLAFIGGIPDPNFIERLQINHVS
AKNSQREDLTKEMEAYPLADSAIWKDKVADPSKITIPAFVIASYSNTLHTMGTFRSWRSLGSKEKWLRIHDRQEWPYYYD
ENNVEELRRFFDYYLLGKENNWTDTPTVRYALIDFQGGNQTDLPSEVFPPTNSENKRYYMNGLLRTLQETSTSDDFKVSY
TTENLPGRASFQMTFDKETHFVGYPKAKLFMEVDGYDDLDVFVWLQKLDKFGNVLSEFVVPNHGAALQDFTQEGASALRY
KGAWGRLRASMRQLDSQVSTDEIPAYSFDKVEKLSSGEIVELDIILSPIGLTYKAGETLRVVVSSKDELGSVMPGTPGCT
PNNKGMHILHTSKEHPSYIQLPIINK

Sequences:

>Translated_586_residues
MTAKITEKMLFSPSFEYVDNGIEHGVLSPFETKEIILEKGQQIAEGFKPLDCSIKMLKDVPVKMRDGITIYTDIYLPITE
EKVPTLIAWSPYGKSAGTAPRYKNLFNMLGMGNAWNSGLTKFEAPDPAYWCAHGYAVCNPDMRGIAHSEGNTTMIGSQEA
EDGYDLIEWLAKQSWSNGKTALTGTSYLAFSQWYIAAEQPPHLTCINPTEGLADGYRDLAFIGGIPDPNFIERLQINHVS
AKNSQREDLTKEMEAYPLADSAIWKDKVADPSKITIPAFVIASYSNTLHTMGTFRSWRSLGSKEKWLRIHDRQEWPYYYD
ENNVEELRRFFDYYLLGKENNWTDTPTVRYALIDFQGGNQTDLPSEVFPPTNSENKRYYMNGLLRTLQETSTSDDFKVSY
TTENLPGRASFQMTFDKETHFVGYPKAKLFMEVDGYDDLDVFVWLQKLDKFGNVLSEFVVPNHGAALQDFTQEGASALRY
KGAWGRLRASMRQLDSQVSTDEIPAYSFDKVEKLSSGEIVELDIILSPIGLTYKAGETLRVVVSSKDELGSVMPGTPGCT
PNNKGMHILHTSKEHPSYIQLPIINK
>Mature_585_residues
TAKITEKMLFSPSFEYVDNGIEHGVLSPFETKEIILEKGQQIAEGFKPLDCSIKMLKDVPVKMRDGITIYTDIYLPITEE
KVPTLIAWSPYGKSAGTAPRYKNLFNMLGMGNAWNSGLTKFEAPDPAYWCAHGYAVCNPDMRGIAHSEGNTTMIGSQEAE
DGYDLIEWLAKQSWSNGKTALTGTSYLAFSQWYIAAEQPPHLTCINPTEGLADGYRDLAFIGGIPDPNFIERLQINHVSA
KNSQREDLTKEMEAYPLADSAIWKDKVADPSKITIPAFVIASYSNTLHTMGTFRSWRSLGSKEKWLRIHDRQEWPYYYDE
NNVEELRRFFDYYLLGKENNWTDTPTVRYALIDFQGGNQTDLPSEVFPPTNSENKRYYMNGLLRTLQETSTSDDFKVSYT
TENLPGRASFQMTFDKETHFVGYPKAKLFMEVDGYDDLDVFVWLQKLDKFGNVLSEFVVPNHGAALQDFTQEGASALRYK
GAWGRLRASMRQLDSQVSTDEIPAYSFDKVEKLSSGEIVELDIILSPIGLTYKAGETLRVVVSSKDELGSVMPGTPGCTP
NNKGMHILHTSKEHPSYIQLPIINK

Specific function: Hydrolyzes cocaine to benzoate and ecgonine methyl ester, endowing the bacteria with the ability to utilize cocaine as a sole source of carbon and energy for growth, as this bacterium lives in the rhizosphere of coca plants [H]

COG id: COG2936

COG function: function code R; Predicted acyl esterases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha/beta hydrolase family. Carboxylesterase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005674
- InterPro:   IPR008979
- InterPro:   IPR013736
- InterPro:   IPR000383 [H]

Pfam domain/function: PF02129 Peptidase_S15; PF08530 PepX_C [H]

EC number: NA

Molecular weight: Translated: 66134; Mature: 66003

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKITEKMLFSPSFEYVDNGIEHGVLSPFETKEIILEKGQQIAEGFKPLDCSIKMLKDV
CCCCHHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHCCCCCCCCHHHHHHCC
PVKMRDGITIYTDIYLPITEEKVPTLIAWSPYGKSAGTAPRYKNLFNMLGMGNAWNSGLT
CEEHHCCEEEEEEEEEEECCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCC
KFEAPDPAYWCAHGYAVCNPDMRGIAHSEGNTTMIGSQEAEDGYDLIEWLAKQSWSNGKT
CCCCCCCCEECCCCEEEECCCCCCCEECCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCE
ALTGTSYLAFSQWYIAAEQPPHLTCINPTEGLADGYRDLAFIGGIPDPNFIERLQINHVS
EEECCCEEEEEEEEEEECCCCCEEEECCCHHHHHHHHHHHEECCCCCCHHHHHHEEEEEC
AKNSQREDLTKEMEAYPLADSAIWKDKVADPSKITIPAFVIASYSNTLHTMGTFRSWRSL
CCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEHEEEEECCCCHHHHHHHHHHHHHC
GSKEKWLRIHDRQEWPYYYDENNVEELRRFFDYYLLGKENNWTDTPTVRYALIDFQGGNQ
CCCCCEEEEECCCCCCEEECCCCHHHHHHHHHHEEECCCCCCCCCCEEEEEEEEECCCCC
TDLPSEVFPPTNSENKRYYMNGLLRTLQETSTSDDFKVSYTTENLPGRASFQMTFDKETH
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEECCCCE
FVGYPKAKLFMEVDGYDDLDVFVWLQKLDKFGNVLSEFVVPNHGAALQDFTQEGASALRY
EECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEE
KGAWGRLRASMRQLDSQVSTDEIPAYSFDKVEKLSSGEIVELDIILSPIGLTYKAGETLR
CCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCEEEEEEEEECCCCEEECCCEEE
VVVSSKDELGSVMPGTPGCTPNNKGMHILHTSKEHPSYIQLPIINK
EEEECCHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCC
>Mature Secondary Structure 
TAKITEKMLFSPSFEYVDNGIEHGVLSPFETKEIILEKGQQIAEGFKPLDCSIKMLKDV
CCCHHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHCCCCCCCCHHHHHHCC
PVKMRDGITIYTDIYLPITEEKVPTLIAWSPYGKSAGTAPRYKNLFNMLGMGNAWNSGLT
CEEHHCCEEEEEEEEEEECCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCC
KFEAPDPAYWCAHGYAVCNPDMRGIAHSEGNTTMIGSQEAEDGYDLIEWLAKQSWSNGKT
CCCCCCCCEECCCCEEEECCCCCCCEECCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCE
ALTGTSYLAFSQWYIAAEQPPHLTCINPTEGLADGYRDLAFIGGIPDPNFIERLQINHVS
EEECCCEEEEEEEEEEECCCCCEEEECCCHHHHHHHHHHHEECCCCCCHHHHHHEEEEEC
AKNSQREDLTKEMEAYPLADSAIWKDKVADPSKITIPAFVIASYSNTLHTMGTFRSWRSL
CCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEHEEEEECCCCHHHHHHHHHHHHHC
GSKEKWLRIHDRQEWPYYYDENNVEELRRFFDYYLLGKENNWTDTPTVRYALIDFQGGNQ
CCCCCEEEEECCCCCCEEECCCCHHHHHHHHHHEEECCCCCCCCCCEEEEEEEEECCCCC
TDLPSEVFPPTNSENKRYYMNGLLRTLQETSTSDDFKVSYTTENLPGRASFQMTFDKETH
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEECCCCE
FVGYPKAKLFMEVDGYDDLDVFVWLQKLDKFGNVLSEFVVPNHGAALQDFTQEGASALRY
EECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEE
KGAWGRLRASMRQLDSQVSTDEIPAYSFDKVEKLSSGEIVELDIILSPIGLTYKAGETLR
CCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCEEEEEEEEECCCCEEECCCEEE
VVVSSKDELGSVMPGTPGCTPNNKGMHILHTSKEHPSYIQLPIINK
EEEECCHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10698749; 11742345; 12369817 [H]