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The map label for this gene is benM [H]

Identifier: 226223118

GI number: 226223118

Start: 526039

End: 526947

Strand: Reverse

Name: benM [H]

Synonym: Lm4b_00515

Alternate gene names: 226223118

Gene position: 526947-526039 (Counterclockwise)

Preceding gene: 226223119

Following gene: 226223114

Centisome position: 18.09

GC content: 32.23

Gene sequence:

>909_bases
ATGAAGTTATCCAATTTGAAATACTTTGTTGATGTGGCTATGGAAGGAAGTTTTACCCGAGCATCTGAAAAATTATTTAT
TTCACAGCCTACCCTTAGTAGACGAATAAAAGAATTAGAAACAGAATTAGGTGTAGAACTGTTTATTAGGAATCGTCATT
CGCTGGAGTTATCAAGTGCAGGTCAACAATTTTTAATAGAAGTAAATGATATTTTAAATCGAGTCAACAAGCTATCCCAT
ATGTTTGATAATCAAAAAACAGCAGATGAAGCTGGACAATTATTAAAGATTGGTTATCTCTCGAATTTCAATATGAACGC
AATGTATGAGCTGCTCGAATCATTCAAGCAAAGTCATCCACATGTTCAATTCTCATTAAAACCGGATACACCAATGAATT
TAGCTGAAGGTCTTTCTAATGGGCAATATGATTTAGTTTTTAATTTATCCGCTTATTTCCAGCCGAACATGTCTATAGAA
GAAGTATTATTTATAAAAAATCATTTGCAAATTGCTATACCCGCGGACCATCGATTCCGGAAAAAGAAAAAAGTATATTT
TAATGATTTGAAGCAAGAAATAGTTATTTTGCTCGAAAGAAAACAGTCTCCCATCATTGTAGACTATGTTGTTAGTCAAT
GTACTAAACATGGTTTTAATTTAAAAGCGAATTCGTATGTAAAAGATCTGGATGAAGGACTAGCAATGACATCTGTTGGA
GAAGGATTAGCCTTTTTGTATTCCGGCATGAATGATGGAACATTAGAAGATAAGTACAATATTAAAATTATGGATATTCA
AGAAGAACGGAATGACCAGAATATTGTCGCAGCCATTAATAAACAGAGCGAAAATACACTGCTTCAAGAATTATTTTCGT
TTATAAAAAGTAGTCTATTAACAAAATAA

Upstream 100 bases:

>100_bases
TTCAGCTACCAATAATCAACAAATAGAAAAGCTTGTCAATGGAGTCTAATAACTTTATACTAAAGCTACTATTTACAATC
TTTCATTGAATGGAGCAATT

Downstream 100 bases:

>100_bases
TTCATTCTAGATAAAAAAAGTAGCAAGTTTCTTTTCATCAAAGAAACTTGCTACTTTTTTAGTATTTATTAAAATTGTTT
GTGCAGTAAATCAAGGACAT

Product: transcription regulator LysR

Products: NA

Alternate protein names: Ben and cat operon transcriptional regulator [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MKLSNLKYFVDVAMEGSFTRASEKLFISQPTLSRRIKELETELGVELFIRNRHSLELSSAGQQFLIEVNDILNRVNKLSH
MFDNQKTADEAGQLLKIGYLSNFNMNAMYELLESFKQSHPHVQFSLKPDTPMNLAEGLSNGQYDLVFNLSAYFQPNMSIE
EVLFIKNHLQIAIPADHRFRKKKKVYFNDLKQEIVILLERKQSPIIVDYVVSQCTKHGFNLKANSYVKDLDEGLAMTSVG
EGLAFLYSGMNDGTLEDKYNIKIMDIQEERNDQNIVAAINKQSENTLLQELFSFIKSSLLTK

Sequences:

>Translated_302_residues
MKLSNLKYFVDVAMEGSFTRASEKLFISQPTLSRRIKELETELGVELFIRNRHSLELSSAGQQFLIEVNDILNRVNKLSH
MFDNQKTADEAGQLLKIGYLSNFNMNAMYELLESFKQSHPHVQFSLKPDTPMNLAEGLSNGQYDLVFNLSAYFQPNMSIE
EVLFIKNHLQIAIPADHRFRKKKKVYFNDLKQEIVILLERKQSPIIVDYVVSQCTKHGFNLKANSYVKDLDEGLAMTSVG
EGLAFLYSGMNDGTLEDKYNIKIMDIQEERNDQNIVAAINKQSENTLLQELFSFIKSSLLTK
>Mature_302_residues
MKLSNLKYFVDVAMEGSFTRASEKLFISQPTLSRRIKELETELGVELFIRNRHSLELSSAGQQFLIEVNDILNRVNKLSH
MFDNQKTADEAGQLLKIGYLSNFNMNAMYELLESFKQSHPHVQFSLKPDTPMNLAEGLSNGQYDLVFNLSAYFQPNMSIE
EVLFIKNHLQIAIPADHRFRKKKKVYFNDLKQEIVILLERKQSPIIVDYVVSQCTKHGFNLKANSYVKDLDEGLAMTSVG
EGLAFLYSGMNDGTLEDKYNIKIMDIQEERNDQNIVAAINKQSENTLLQELFSFIKSSLLTK

Specific function: Positive regulator of the ben and cat genes for benzoate degradation. BenM is necessary for ben gene expression but not for expression of the cat genes, which can be regulated by CatM. Binds to the inducers cis,cis-muconate and benzoate [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI2367136, Length=266, Percent_Identity=27.8195488721804, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788887, Length=185, Percent_Identity=29.7297297297297, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1787879, Length=269, Percent_Identity=26.3940520446097, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787601, Length=240, Percent_Identity=27.5, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790399, Length=246, Percent_Identity=25.2032520325203, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787530, Length=199, Percent_Identity=25.6281407035176, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787806, Length=147, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI157672245, Length=258, Percent_Identity=24.8062015503876, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87081904, Length=200, Percent_Identity=25.5, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788296, Length=182, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789173, Length=120, Percent_Identity=31.6666666666667, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786401, Length=193, Percent_Identity=27.979274611399, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786713, Length=149, Percent_Identity=28.8590604026846, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790208, Length=152, Percent_Identity=30.2631578947368, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1788748, Length=142, Percent_Identity=28.169014084507, Blast_Score=61, Evalue=9e-11,
Organism=Escherichia coli, GI145693105, Length=189, Percent_Identity=24.3386243386243, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34636; Mature: 34636

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSNLKYFVDVAMEGSFTRASEKLFISQPTLSRRIKELETELGVELFIRNRHSLELSSA
CCCCCEEEEEEEEECCCCCCCCCEEEEECCHHHHHHHHHHHHCCEEEEEECCCCEEHHCC
GQQFLIEVNDILNRVNKLSHMFDNQKTADEAGQLLKIGYLSNFNMNAMYELLESFKQSHP
CHHEEEEHHHHHHHHHHHHHHHCCCCCHHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCC
HVQFSLKPDTPMNLAEGLSNGQYDLVFNLSAYFQPNMSIEEVLFIKNHLQIAIPADHRFR
CEEEEECCCCCHHHHHHCCCCCEEEEEEEEEEECCCCCHHHHHEEECCEEEEECCHHHHH
KKKKVYFNDLKQEIVILLERKQSPIIVDYVVSQCTKHGFNLKANSYVKDLDEGLAMTSVG
HHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHCCCCCCCHHHHHHHHHCHHHHHHC
EGLAFLYSGMNDGTLEDKYNIKIMDIQEERNDQNIVAAINKQSENTLLQELFSFIKSSLL
CCHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHC
TK
CC
>Mature Secondary Structure
MKLSNLKYFVDVAMEGSFTRASEKLFISQPTLSRRIKELETELGVELFIRNRHSLELSSA
CCCCCEEEEEEEEECCCCCCCCCEEEEECCHHHHHHHHHHHHCCEEEEEECCCCEEHHCC
GQQFLIEVNDILNRVNKLSHMFDNQKTADEAGQLLKIGYLSNFNMNAMYELLESFKQSHP
CHHEEEEHHHHHHHHHHHHHHHCCCCCHHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCC
HVQFSLKPDTPMNLAEGLSNGQYDLVFNLSAYFQPNMSIEEVLFIKNHLQIAIPADHRFR
CEEEEECCCCCHHHHHHCCCCCEEEEEEEEEEECCCCCHHHHHEEECCEEEEECCHHHHH
KKKKVYFNDLKQEIVILLERKQSPIIVDYVVSQCTKHGFNLKANSYVKDLDEGLAMTSVG
HHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHCCCCCCCHHHHHHHHHCHHHHHHC
EGLAFLYSGMNDGTLEDKYNIKIMDIQEERNDQNIVAAINKQSENTLLQELFSFIKSSLL
CCHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHC
TK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9573203 [H]