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The map label for this gene is thiD [H]

Identifier: 226222944

GI number: 226222944

Start: 340005

End: 340808

Strand: Direct

Name: thiD [H]

Synonym: Lm4b_00336

Alternate gene names: 226222944

Gene position: 340005-340808 (Clockwise)

Preceding gene: 226222943

Following gene: 226222945

Centisome position: 11.67

GC content: 45.65

Gene sequence:

>804_bases
ATGATTTTCAAACAAGTATTAACGATAGCTGGTTCAGATTCTGGCGGCGGCGCGGGGATACAAGCGGATATCAAGACATT
TCAAGAGCGTAAAACCTTTGGCATGTCCGTCATCACGGCAATCACGGCACAAAACACGCTAGGTGTAAAAGCAGTGCATA
AAATCCCGGTAGAAATGATCCGCGAACAGTGCGACGCGATTGCAGAGGATTTTCAAGTGAGCGCCGTGAAAACTGGAATG
CTAGCAGATGCAGAAATTATCCGGGAAGTGGCGCGGAATATCCGTTTGCACAACTTCCCAAATATCGTCATTGATCCTGT
GATGATTGCAAAAGGTGGAACTGCTTTGCTTGAAAACGAGGCGACCCAAGTGTTGAAAGACGAGCTTTTGCCACTTGGTA
CGATCATTACGCCGAATATTCCAGAAGCGGAAGAAATTCTTGGTGAAAAAATCACAACCAAGGCGGAAATTGAACAAGCC
GCTAAGAAAATTTTTGATTTAGGTGTAAAAGCGGTTGTTATTAAAGGTGGGCATAGCGAAATGAGTGAGGCGGCTGATTT
TTACTATGATGGCGAAACGACAAAATGGCTGACGAGCGAGCGCTTTGATACGCCGCATACGCACGGGACTGGTTGCACTT
TTTCGGCATGTATTGCAGCTGAACTTGCGAAAGGGAATTCGCTTTTGGATAGTGTCATGGTTGCGAAAGAATTTATCACA
AGTGCGATTAAATATCCGCTTGGAATTGGTCACGGTCATGGTCCAACCAACCATTTTGCCTACCGATTGGAGGATGGGAA
ATGA

Upstream 100 bases:

>100_bases
TTCCAGGTTCATTTCGACCACAATTTTTAGATGCTCTTGCTAGTTGGTCGGTCGAAAAAACGCGGGCCAAAGCCAAAATC
CAAGAAAGTGGTGAACACAA

Downstream 100 bases:

>100_bases
GAGCTGAACTAGCTGTATATTTTATCGCTGGAACGCAAGATATTGTTCGTGGAACGTTGCCAGGTGTGTTAGAAGAAGCA
CTGAAAGCCGGAATTACTTG

Product: phosphomethylpyrimidine kinase (ThiD)

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MIFKQVLTIAGSDSGGGAGIQADIKTFQERKTFGMSVITAITAQNTLGVKAVHKIPVEMIREQCDAIAEDFQVSAVKTGM
LADAEIIREVARNIRLHNFPNIVIDPVMIAKGGTALLENEATQVLKDELLPLGTIITPNIPEAEEILGEKITTKAEIEQA
AKKIFDLGVKAVVIKGGHSEMSEAADFYYDGETTKWLTSERFDTPHTHGTGCTFSACIAAELAKGNSLLDSVMVAKEFIT
SAIKYPLGIGHGHGPTNHFAYRLEDGK

Sequences:

>Translated_267_residues
MIFKQVLTIAGSDSGGGAGIQADIKTFQERKTFGMSVITAITAQNTLGVKAVHKIPVEMIREQCDAIAEDFQVSAVKTGM
LADAEIIREVARNIRLHNFPNIVIDPVMIAKGGTALLENEATQVLKDELLPLGTIITPNIPEAEEILGEKITTKAEIEQA
AKKIFDLGVKAVVIKGGHSEMSEAADFYYDGETTKWLTSERFDTPHTHGTGCTFSACIAAELAKGNSLLDSVMVAKEFIT
SAIKYPLGIGHGHGPTNHFAYRLEDGK
>Mature_267_residues
MIFKQVLTIAGSDSGGGAGIQADIKTFQERKTFGMSVITAITAQNTLGVKAVHKIPVEMIREQCDAIAEDFQVSAVKTGM
LADAEIIREVARNIRLHNFPNIVIDPVMIAKGGTALLENEATQVLKDELLPLGTIITPNIPEAEEILGEKITTKAEIEQA
AKKIFDLGVKAVVIKGGHSEMSEAADFYYDGETTKWLTSERFDTPHTHGTGCTFSACIAAELAKGNSLLDSVMVAKEFIT
SAIKYPLGIGHGHGPTNHFAYRLEDGK

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=256, Percent_Identity=40.625, Blast_Score=202, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6325378, Length=269, Percent_Identity=33.457249070632, Blast_Score=141, Evalue=9e-35,
Organism=Saccharomyces cerevisiae, GI6324997, Length=268, Percent_Identity=33.5820895522388, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6324517, Length=268, Percent_Identity=33.2089552238806, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 28769; Mature: 28769

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFKQVLTIAGSDSGGGAGIQADIKTFQERKTFGMSVITAITAQNTLGVKAVHKIPVEMI
CCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
REQCDAIAEDFQVSAVKTGMLADAEIIREVARNIRLHNFPNIVIDPVMIAKGGTALLENE
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCEEECCEEEECCCCHHHHHH
ATQVLKDELLPLGTIITPNIPEAEEILGEKITTKAEIEQAAKKIFDLGVKAVVIKGGHSE
HHHHHHHHCCCCCCEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEECCCHH
MSEAADFYYDGETTKWLTSERFDTPHTHGTGCTFSACIAAELAKGNSLLDSVMVAKEFIT
HHHHHHEEECCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHH
SAIKYPLGIGHGHGPTNHFAYRLEDGK
HHHHCCCCCCCCCCCCCCEEEEECCCC
>Mature Secondary Structure
MIFKQVLTIAGSDSGGGAGIQADIKTFQERKTFGMSVITAITAQNTLGVKAVHKIPVEMI
CCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
REQCDAIAEDFQVSAVKTGMLADAEIIREVARNIRLHNFPNIVIDPVMIAKGGTALLENE
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCEEECCEEEECCCCHHHHHH
ATQVLKDELLPLGTIITPNIPEAEEILGEKITTKAEIEQAAKKIFDLGVKAVVIKGGHSE
HHHHHHHHCCCCCCEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEECCCHH
MSEAADFYYDGETTKWLTSERFDTPHTHGTGCTFSACIAAELAKGNSLLDSVMVAKEFIT
HHHHHHEEECCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHH
SAIKYPLGIGHGHGPTNHFAYRLEDGK
HHHHCCCCCCCCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]