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The map label for this gene is thiE

Identifier: 226222945

GI number: 226222945

Start: 340805

End: 341449

Strand: Direct

Name: thiE

Synonym: Lm4b_00337

Alternate gene names: 226222945

Gene position: 340805-341449 (Clockwise)

Preceding gene: 226222944

Following gene: 226222948

Centisome position: 11.7

GC content: 46.36

Gene sequence:

>645_bases
ATGAGAGCTGAACTAGCTGTATATTTTATCGCTGGAACGCAAGATATTGTTCGTGGAACGTTGCCAGGTGTGTTAGAAGA
AGCACTGAAAGCCGGAATTACTTGTTTCCAATATCGCGAAAAAGGAGCAGGCTCACTCCAAACTGCTTCCGAAAGAAAAG
AAATGGCGCTAGAATGCCAACAATTATGCGCAAAATATCAAGTGCCATTCATTATTAACGATGATGTCGCCCTAGCCCTC
GAAATCGGCGCGGACGGCATCCATGTCGGGCAAAACGACGAGGAAATTCGCCAAGTTATCGCGAGTTGCGCTGGAAAAAT
GAAAATCGGACTTTCGGTTCATTCGGTTAGTGAAGCGGAGGAAGCAGAACGACTTGGTGCGGTGGATTATATTGGTGTAG
GGCCCATTTTTCCAACGATTTCAAAAGCGGATGCAGAGCCAGTGAGTGGAACGGCAATTTTGGAAGAAATTCGCCGGGCT
GGAATTAAGCTACCAATTGTCGGGATTGGCGGAATTAATGAAACAAATTCAGCTGAGGTTCTCACAGCAGGTGCGGACGG
GGTATCGGTTATTTCAGCGATAACTCGGTCGGATGATTGCTACTCCGTTATCAAGCAATTAAAAAACCCAGGTTACCCCT
CTTAA

Upstream 100 bases:

>100_bases
TTGCGAAAGAATTTATCACAAGTGCGATTAAATATCCGCTTGGAATTGGTCACGGTCATGGTCCAACCAACCATTTTGCC
TACCGATTGGAGGATGGGAA

Downstream 100 bases:

>100_bases
AAAGGGCAAACCTGGGTTTCTCATTTTTATAAATCTTCACCATTGGTAGCAATAACGTTCTTGTACCAATCAAAACTTTT
CTTCTTATAACGGTTTAGCG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MRAELAVYFIAGTQDIVRGTLPGVLEEALKAGITCFQYREKGAGSLQTASERKEMALECQQLCAKYQVPFIINDDVALAL
EIGADGIHVGQNDEEIRQVIASCAGKMKIGLSVHSVSEAEEAERLGAVDYIGVGPIFPTISKADAEPVSGTAILEEIRRA
GIKLPIVGIGGINETNSAEVLTAGADGVSVISAITRSDDCYSVIKQLKNPGYPS

Sequences:

>Translated_214_residues
MRAELAVYFIAGTQDIVRGTLPGVLEEALKAGITCFQYREKGAGSLQTASERKEMALECQQLCAKYQVPFIINDDVALAL
EIGADGIHVGQNDEEIRQVIASCAGKMKIGLSVHSVSEAEEAERLGAVDYIGVGPIFPTISKADAEPVSGTAILEEIRRA
GIKLPIVGIGGINETNSAEVLTAGADGVSVISAITRSDDCYSVIKQLKNPGYPS
>Mature_214_residues
MRAELAVYFIAGTQDIVRGTLPGVLEEALKAGITCFQYREKGAGSLQTASERKEMALECQQLCAKYQVPFIINDDVALAL
EIGADGIHVGQNDEEIRQVIASCAGKMKIGLSVHSVSEAEEAERLGAVDYIGVGPIFPTISKADAEPVSGTAILEEIRRA
GIKLPIVGIGGINETNSAEVLTAGADGVSVISAITRSDDCYSVIKQLKNPGYPS

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=192, Percent_Identity=30.7291666666667, Blast_Score=72, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6325042, Length=224, Percent_Identity=35.2678571428571, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 22595; Mature: 22595

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAELAVYFIAGTQDIVRGTLPGVLEEALKAGITCFQYREKGAGSLQTASERKEMALECQ
CCCEEEEEEEECCHHHHHHCCHHHHHHHHHHCCHHEEHHHCCCCCCHHHHHHHHHHHHHH
QLCAKYQVPFIINDDVALALEIGADGIHVGQNDEEIRQVIASCAGKMKIGLSVHSVSEAE
HHHHHCCCCEEECCCEEEEEEECCCCEECCCCHHHHHHHHHHHCCCEEECEEEHHHHHHH
EAERLGAVDYIGVGPIFPTISKADAEPVSGTAILEEIRRAGIKLPIVGIGGINETNSAEV
HHHHCCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCEE
LTAGADGVSVISAITRSDDCYSVIKQLKNPGYPS
EEECCCHHHHHHHHHCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRAELAVYFIAGTQDIVRGTLPGVLEEALKAGITCFQYREKGAGSLQTASERKEMALECQ
CCCEEEEEEEECCHHHHHHCCHHHHHHHHHHCCHHEEHHHCCCCCCHHHHHHHHHHHHHH
QLCAKYQVPFIINDDVALALEIGADGIHVGQNDEEIRQVIASCAGKMKIGLSVHSVSEAE
HHHHHCCCCEEECCCEEEEEEECCCCEECCCCHHHHHHHHHHHCCCEEECEEEHHHHHHH
EAERLGAVDYIGVGPIFPTISKADAEPVSGTAILEEIRRAGIKLPIVGIGGINETNSAEV
HHHHCCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCEE
LTAGADGVSVISAITRSDDCYSVIKQLKNPGYPS
EEECCCHHHHHHHHHCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]