Click here to switch to the map view.

The map label for this gene is xasA [C]

Identifier: 226222682

GI number: 226222682

Start: 47986

End: 49371

Strand: Direct

Name: xasA [C]

Synonym: Lm4b_00046

Alternate gene names: 226222682

Gene position: 47986-49371 (Clockwise)

Preceding gene: 226222681

Following gene: 226222683

Centisome position: 1.65

GC content: 37.3

Gene sequence:

>1386_bases
TTGGAAAAAAAGAAGAAATTCAGATTATTTGATGCGGTTCTTATGGCTGTATGTGTCGTTTTAGTTGTGGAATCTGCCGC
GCCAGCAGCGGCGATAGGATCATCTCAATTTTTCTGGTGGATTGCGTTACTTATTTTATTTTTCTTACCATATGGACTTG
TTTCGGCAGAACTTGGGACAACCTATGATGATGAAGGCGGAATCTATGATTGGGTAAAACGGGCATTTGGCCGAAAATGG
GGTGCGCGTGTCGCTTGGTTATACTGGATTAATTATCCGATTTGGATGGCGAGTTTAGCGGTATTGTTTGTAGAAGTTAT
TACGCAAATTTTCCCGGTTTCATTTGGGACGCCGGTTTCGATTTTGATTCAGCTCATTTTTGTGTGGATTGTTGTTATTA
TTAGTTGTTATCCAGTGAGTGATAGCAAATGGATTCTTAATATCGCAGCTTTCTGTAAAGTGGCAATTATGCTTTGCCTT
GGTGTACTTGGAATTTATTTTGCGATGACGAAGGGATTGGCGAATGATTTCTCTGGAAAAGCATTACTTCCAACTTTTGA
TTTAGAGAGTTTGTCATTTATTTCAGTCATTTTATTTAATTTCTTAGGTTTTGAAGTGGTAACAACGCTTGCGAGTGACA
TGGAAAATCCTAAAAAACAAATTCCTCAAGCAATCATTTATGGTGGGGTTTTGATTGCATTCTTCTATTTACTAGCAGCT
TTCGGAATGGGGGCAGCAATTCCGACTTCTGAACTTTCAACATCCGGTGGTCTTATTGATAGCTTTATTATGCTGATTGG
CGGAGTTAACCCATTTGTTATTATTATTGGGATTATGTTTATGTATACATTAGTAGCAAATTTAGTCTCTTGGGCACTAG
GCGTGAATTATGTAGCAATGTATGCAGCGAAAAACAAAGATTTACCAGCTGTTTTTGGAAAAACAAATCCGAAAAATGAT
ATGCCAACGGGTACTAGTATTTTAAATGGGATTGTAGCTTCTGTATTAATCGTTGCGGCACCACTTATTCCAAATGAAAA
TATTTTCTGGGCATTCTTTGCATTAAATGTAGTTGCTTTACTAGGTTCATATATCCTAATGTTTCCAGCATTTTTGAAAT
TACGTAAGGTGGATCCGGACCGAGAACGTCCATTTAAAGTGCCAGGTGGCAAAATCTTGCTTTACTTAATGACTTTCGTA
CCAATGATTTTACTCATTATTACTTTGATTTTCTCTGCAGTACCATTAAATGGTTCAAGTGCAGAATTAAATGAAAAAAT
CCCAATCTTAATTGGAACCGTTGTAGCGGTGATTGTTGGTGAAATATGTATTTGGCTTGCTGGGAAAAGAAAAGACAAAG
GAGAGATAGGAAATGAGAACGATTGA

Upstream 100 bases:

>100_bases
ATTACTTAGAAATGGCGCTGGCTTATAAGATACCAAAATAAGGAGGAGAAGAGAGCGCTGGCCGTTTTCTTCCCCCCACT
TAAAAAGAGGAGTGGGCATT

Downstream 100 bases:

>100_bases
TAGTTCTTCTAAAAAAGATGGATTTAGAATGCCTGGGGAATTTGAAAAACACGCTGGTTGCTATATTATTTGGCCAGAAC
GACCAGATAATTGGCGCTTA

Product: amino acid transporter

Products: L-glutamate [Cytoplasm]; 4-aminobutyrate [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MEKKKKFRLFDAVLMAVCVVLVVESAAPAAAIGSSQFFWWIALLILFFLPYGLVSAELGTTYDDEGGIYDWVKRAFGRKW
GARVAWLYWINYPIWMASLAVLFVEVITQIFPVSFGTPVSILIQLIFVWIVVIISCYPVSDSKWILNIAAFCKVAIMLCL
GVLGIYFAMTKGLANDFSGKALLPTFDLESLSFISVILFNFLGFEVVTTLASDMENPKKQIPQAIIYGGVLIAFFYLLAA
FGMGAAIPTSELSTSGGLIDSFIMLIGGVNPFVIIIGIMFMYTLVANLVSWALGVNYVAMYAAKNKDLPAVFGKTNPKND
MPTGTSILNGIVASVLIVAAPLIPNENIFWAFFALNVVALLGSYILMFPAFLKLRKVDPDRERPFKVPGGKILLYLMTFV
PMILLIITLIFSAVPLNGSSAELNEKIPILIGTVVAVIVGEICIWLAGKRKDKGEIGNEND

Sequences:

>Translated_461_residues
MEKKKKFRLFDAVLMAVCVVLVVESAAPAAAIGSSQFFWWIALLILFFLPYGLVSAELGTTYDDEGGIYDWVKRAFGRKW
GARVAWLYWINYPIWMASLAVLFVEVITQIFPVSFGTPVSILIQLIFVWIVVIISCYPVSDSKWILNIAAFCKVAIMLCL
GVLGIYFAMTKGLANDFSGKALLPTFDLESLSFISVILFNFLGFEVVTTLASDMENPKKQIPQAIIYGGVLIAFFYLLAA
FGMGAAIPTSELSTSGGLIDSFIMLIGGVNPFVIIIGIMFMYTLVANLVSWALGVNYVAMYAAKNKDLPAVFGKTNPKND
MPTGTSILNGIVASVLIVAAPLIPNENIFWAFFALNVVALLGSYILMFPAFLKLRKVDPDRERPFKVPGGKILLYLMTFV
PMILLIITLIFSAVPLNGSSAELNEKIPILIGTVVAVIVGEICIWLAGKRKDKGEIGNEND
>Mature_461_residues
MEKKKKFRLFDAVLMAVCVVLVVESAAPAAAIGSSQFFWWIALLILFFLPYGLVSAELGTTYDDEGGIYDWVKRAFGRKW
GARVAWLYWINYPIWMASLAVLFVEVITQIFPVSFGTPVSILIQLIFVWIVVIISCYPVSDSKWILNIAAFCKVAIMLCL
GVLGIYFAMTKGLANDFSGKALLPTFDLESLSFISVILFNFLGFEVVTTLASDMENPKKQIPQAIIYGGVLIAFFYLLAA
FGMGAAIPTSELSTSGGLIDSFIMLIGGVNPFVIIIGIMFMYTLVANLVSWALGVNYVAMYAAKNKDLPAVFGKTNPKND
MPTGTSILNGIVASVLIVAAPLIPNENIFWAFFALNVVALLGSYILMFPAFLKLRKVDPDRERPFKVPGGKILLYLMTFV
PMILLIITLIFSAVPLNGSSAELNEKIPILIGTVVAVIVGEICIWLAGKRKDKGEIGNEND

Specific function: Probable Amino Acid Transporter. Involved In Extreme Acid Resistance. [C]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Homo sapiens, GI186910308, Length=422, Percent_Identity=22.2748815165877, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI186910306, Length=422, Percent_Identity=22.2748815165877, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI186910304, Length=422, Percent_Identity=22.2748815165877, Blast_Score=70, Evalue=6e-12,
Organism=Escherichia coli, GI1787768, Length=440, Percent_Identity=25.2272727272727, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI87082380, Length=272, Percent_Identity=26.1029411764706, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI87081798, Length=420, Percent_Identity=21.6666666666667, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI2367353, Length=367, Percent_Identity=23.1607629427793, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 50536; Mature: 50536

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure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CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-aminobutyrate [Cytoplasm]; L-glutamate [Periplasm] [C]

Specific reaction: 4-aminobutyrate [Cytoplasm] + L-glutamate [Periplasm] = L-glutamate [Cytoplasm] + 4-aminobutyrate [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8366052 [H]