Click here to switch to the map view.

The map label for this gene is ptcA

Identifier: 226222681

GI number: 226222681

Start: 46888

End: 47913

Strand: Direct

Name: ptcA

Synonym: Lm4b_00045

Alternate gene names: 226222681

Gene position: 46888-47913 (Clockwise)

Preceding gene: 226222680

Following gene: 226222682

Centisome position: 1.61

GC content: 37.91

Gene sequence:

>1026_bases
ATGAACAAAAAAAGAGATTTTATCGACACTAAAGATTTTTCTAAAGAAGAAATACTATTTATGATAGAAATCGGTCGCAA
AATGAAAGAATCTATTAAAAACGGTCATTACCCACAACTTTTAAAACATAAGACGCTTGGGATGATTTTTGAACAATCAT
CAACAAGAACTCGTGTATCTTTTGAAACAGCAATGACACAGCTTGGTGGTCATGCGCAATATTTAGCTCCTGGACAAATT
CAGCTCGGCGGACATGAATCAGTAGGCGATACAGCCAAAGTTCTTTCTAGGCTTGTAGATATTTTGATGGCGCGTGTTGA
ACGTCATCAAACGGTAGTAGAACTTGCAAACACAGCTGCTATTCCGGTTATTAATGGGATGAGTGATTACAACCATCCTA
CACAAGAATTAGGTGATGCAATTACTATGTTCGAACACCTTCCAAAAGGAAAAAAGATTGAAGACTGCAAAATTGTCTTT
GTAGGAGATGCTACTCAAGTATGTGCATCTACAATGTTTATGGCAACAAAACTTGGAATGGATTTCGTTCAATTTGGACC
AAAAGGATTTCAATTACGTGAAGAACATTTGAAAATCGCTGAAGAAAATTGCAAAGTATCTGGCGGGAGCTATTTAATTA
CAGAAGATGTGGACATTGCGTTAAAAGACGCAGATTTCATTTATACAGATGTTTGGTATGGCCTATACGAAGCGGAACTT
TCCGAAGAAGAGCGAATGAAAACTTTTTATCCTAAATATCAAGTGAATAAAGAATTGATCAGTAAAGCGGCTCCACATGT
GAAGTTTATGCACTGTTTACCCGCAACTCGTGGGGAAGAGGTTACGGACGAAGTGCTTGATGCTCCATACTCAGTTGTTA
TTGACGAAGCTGAAAATCGTTTAACAGCGATGAGAGCACTGCTTGTATACTTTATGAATCCGTATGTGAGAGAAGCGGGT
TTTGCAGTTGCGGAAAAATATGATGCAGAACTAGAGTTATTACTTAGAAATGGCGCTGGCTTATAA

Upstream 100 bases:

>100_bases
TCAAAAACAATCTCTTTTGTACTATGATGAGCATGTGGCAAGGAAATACTCGAGTATGCCCCAGAAAAGGTTTAGAAAAT
AAATGGTTGAGGTGATAGAA

Downstream 100 bases:

>100_bases
GATACCAAAATAAGGAGGAGAAGAGAGCGCTGGCCGTTTTCTTCCCCCCACTTAAAAAGAGGAGTGGGCATTTTGGAAAA
AAAGAAGAAATTCAGATTAT

Product: putrescine carbamoyltransferase

Products: NA

Alternate protein names: PTC; PTCase; Putrescine transcarbamoylase; Putrescine transcarbamylase

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MNKKRDFIDTKDFSKEEILFMIEIGRKMKESIKNGHYPQLLKHKTLGMIFEQSSTRTRVSFETAMTQLGGHAQYLAPGQI
QLGGHESVGDTAKVLSRLVDILMARVERHQTVVELANTAAIPVINGMSDYNHPTQELGDAITMFEHLPKGKKIEDCKIVF
VGDATQVCASTMFMATKLGMDFVQFGPKGFQLREEHLKIAEENCKVSGGSYLITEDVDIALKDADFIYTDVWYGLYEAEL
SEEERMKTFYPKYQVNKELISKAAPHVKFMHCLPATRGEEVTDEVLDAPYSVVIDEAENRLTAMRALLVYFMNPYVREAG
FAVAEKYDAELELLLRNGAGL

Sequences:

>Translated_341_residues
MNKKRDFIDTKDFSKEEILFMIEIGRKMKESIKNGHYPQLLKHKTLGMIFEQSSTRTRVSFETAMTQLGGHAQYLAPGQI
QLGGHESVGDTAKVLSRLVDILMARVERHQTVVELANTAAIPVINGMSDYNHPTQELGDAITMFEHLPKGKKIEDCKIVF
VGDATQVCASTMFMATKLGMDFVQFGPKGFQLREEHLKIAEENCKVSGGSYLITEDVDIALKDADFIYTDVWYGLYEAEL
SEEERMKTFYPKYQVNKELISKAAPHVKFMHCLPATRGEEVTDEVLDAPYSVVIDEAENRLTAMRALLVYFMNPYVREAG
FAVAEKYDAELELLLRNGAGL
>Mature_341_residues
MNKKRDFIDTKDFSKEEILFMIEIGRKMKESIKNGHYPQLLKHKTLGMIFEQSSTRTRVSFETAMTQLGGHAQYLAPGQI
QLGGHESVGDTAKVLSRLVDILMARVERHQTVVELANTAAIPVINGMSDYNHPTQELGDAITMFEHLPKGKKIEDCKIVF
VGDATQVCASTMFMATKLGMDFVQFGPKGFQLREEHLKIAEENCKVSGGSYLITEDVDIALKDADFIYTDVWYGLYEAEL
SEEERMKTFYPKYQVNKELISKAAPHVKFMHCLPATRGEEVTDEVLDAPYSVVIDEAENRLTAMRALLVYFMNPYVREAG
FAVAEKYDAELELLLRNGAGL

Specific function: Catalyzes the phosphorolysis of N-carbamoylputrescine to form carbamoyl phosphate and putrescine. Is involved in the degradation pathway of the polyamine agmatine

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family. PTCase subfamily

Homologues:

Organism=Homo sapiens, GI38788445, Length=314, Percent_Identity=38.8535031847134, Blast_Score=209, Evalue=4e-54,
Organism=Escherichia coli, GI1786469, Length=326, Percent_Identity=37.1165644171779, Blast_Score=205, Evalue=3e-54,
Organism=Escherichia coli, GI1790703, Length=329, Percent_Identity=37.0820668693009, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI48994908, Length=315, Percent_Identity=24.4444444444444, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI2367364, Length=318, Percent_Identity=24.8427672955975, Blast_Score=88, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI193204318, Length=161, Percent_Identity=30.4347826086957, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322373, Length=326, Percent_Identity=33.4355828220859, Blast_Score=171, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6322331, Length=294, Percent_Identity=24.1496598639456, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTC_LISMC (C1KV77)

Other databases:

- EMBL:   FM242711
- RefSeq:   YP_002756788.1
- ProteinModelPortal:   C1KV77
- SMR:   C1KV77
- GeneID:   7702045
- GenomeReviews:   FM242711_GR
- KEGG:   lmc:Lm4b_00045
- OMA:   GGHETIE
- ProtClustDB:   PRK02255
- HAMAP:   MF_02102
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.6

Molecular weight: Translated: 38470; Mature: 38470

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 105-105 BINDING 132-132

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKRDFIDTKDFSKEEILFMIEIGRKMKESIKNGHYPQLLKHKTLGMIFEQSSTRTRVS
CCCCCCCCCCCCCCHHHEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEECCCCCEEEH
FETAMTQLGGHAQYLAPGQIQLGGHESVGDTAKVLSRLVDILMARVERHQTVVELANTAA
HHHHHHHHCCCCEEECCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IPVINGMSDYNHPTQELGDAITMFEHLPKGKKIEDCKIVFVGDATQVCASTMFMATKLGM
CCEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCC
DFVQFGPKGFQLREEHLKIAEENCKVSGGSYLITEDVDIALKDADFIYTDVWYGLYEAEL
HHHHHCCCCCHHHHHHHHHHHHCCEECCCCEEEECCCEEEEECCCEEHHHHHHHHHHHHC
SEEERMKTFYPKYQVNKELISKAAPHVKFMHCLPATRGEEVTDEVLDAPYSVVIDEAENR
CHHHHHHHHCCCHHCCHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHCCCCEEEEEHHHHH
LTAMRALLVYFMNPYVREAGFAVAEKYDAELELLLRNGAGL
HHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHCCCCC
>Mature Secondary Structure
MNKKRDFIDTKDFSKEEILFMIEIGRKMKESIKNGHYPQLLKHKTLGMIFEQSSTRTRVS
CCCCCCCCCCCCCCHHHEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEECCCCCEEEH
FETAMTQLGGHAQYLAPGQIQLGGHESVGDTAKVLSRLVDILMARVERHQTVVELANTAA
HHHHHHHHCCCCEEECCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IPVINGMSDYNHPTQELGDAITMFEHLPKGKKIEDCKIVFVGDATQVCASTMFMATKLGM
CCEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCC
DFVQFGPKGFQLREEHLKIAEENCKVSGGSYLITEDVDIALKDADFIYTDVWYGLYEAEL
HHHHHCCCCCHHHHHHHHHHHHCCEECCCCEEEECCCEEEEECCCEEHHHHHHHHHHHHC
SEEERMKTFYPKYQVNKELISKAAPHVKFMHCLPATRGEEVTDEVLDAPYSVVIDEAENR
CHHHHHHHHCCCHHCCHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHCCCCEEEEEHHHHH
LTAMRALLVYFMNPYVREAGFAVAEKYDAELELLLRNGAGL
HHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA