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The map label for this gene is cls1 [H]

Identifier: 226222647

GI number: 226222647

Start: 10727

End: 12241

Strand: Direct

Name: cls1 [H]

Synonym: Lm4b_00008

Alternate gene names: 226222647

Gene position: 10727-12241 (Clockwise)

Preceding gene: 226222646

Following gene: 226222648

Centisome position: 0.37

GC content: 37.49

Gene sequence:

>1515_bases
ATGCGCAAACTGATTCAATTTCTGTTTATAGCAGTAGTTTTATTTTTAGTGGAATACTTTTTAATCAATCAAGCAGCTAT
ATTATTCTTAGTAACAAGTGGACTTATCCAATTATGCGGATTAATTATTGTGATTCGGCTACTACTCTTTGATCAACGGA
ATACTAGCTCAAAAGTCGCCTGGATTGCGGTTATTTTCATTTTACCTGTACTTGGAACGATTAGTTACCTTGTGTTTGGT
AGAAATCCAGCAACTAGAAAATTCAGCACAGCTCAAGTAATGGAAAAAGCTAAATTAATTAATGCGATTCACGCAATTCC
AAACAACACTAATGAAAAATTACCACGACTATCTAAAAGAATTGCGCATTTAACGTCCATAGAGCCAATTAAGGGGAATA
AAATCGAAATATTAACAAATGGCGAAGAAACTTTTCCAGTGCTCTTAGACGCGCTTAGAAAGGCTGAAAACCACATTCAT
ATTCAATACTATATTTTCAAAACGGATGAAATTTCTACAAAGATTCGTGATATTTTGGTAGAAAAAGCAAAAGCAGGCGT
TGAAGTTAGATTTATGTTTGATGGGCTTGGATCAAGCAAACTGGGTAAAGCCTTTTTAGCTCCTTTGAAAGAAGCTGGAG
TTAGTATTCACGCATTTGACCCAATTACCTCTCCCTGGATTGTAAGAACAGCCAATTTAAGAAATCACCGTAAAATTGTC
GTGATTGATGGTCAAATTGGCTTTACAGGCGGGCTTAATATTGGAGAAGAATATCGTTCTAATACACCAGATTTCCGTGT
TTGGCGCGATACACATATTAAAATCACAGGCCAAGCTGTTATCGAACTTCAAGAATCTTTTCTAAATGATTGGGTCTATA
TGGAAAACCAAGCAGGTGCTGCAGATCAATTTATTAGTGAAGCTGGATTACAACAATATTTTTCACCAGTTGATGTGGGT
GACGAATGGGCGCAAGTGATTTACGGCGGACCATATGATAAAGAAAAATGGGTTCGCGATTCGATGCTTGATTTAATTGA
TTCCGCTAAAGAATCTGTTTGGATTGTGTCGCCTTACTTTGTTCCAGATGAGGAGTCGCTTGCAGTTATTCGCCGGGTTG
CGATGAGCGGTGTTGACGTGCGAGTTATTATTCCAGGCAAAGGTGATCGCGGGATTTCATTCCACGGAAGTAATGCGTAT
GTGAAAACAATGATTGAAGCAGGCGCGAAAATGTATGCTTATGCCGATGATTCTTTTGTTCATGCGAAGGCAATGCTAGT
GGACGGGACGCGTGCAGCTATAGGTACTGCCAATTTTGACGTGCGTAGTTTTAGATTGAATCATGAATTAATGGTATTCT
TATATGATGAAAGCGAGGCTATGCATCATTTAAAACGTGATTTTGAGAAAGATTTTGAAGATAGCCGACTTTTTACGATG
AAAGATATGGAAAACAAACCATTATTGACTCGTATAAAAGAAGTTCTATCTAGTTTACTATCACCAATTTTATAA

Upstream 100 bases:

>100_bases
AGAACGATTTTCCAGCTTTTTTCCAGTATAAAAAGCGCTTTTTGGCTATAATTTATGTATAATATCCTTAATACGTCTTA
AAAAGGGAAGAGTTGAACAA

Downstream 100 bases:

>100_bases
TTTAGGAGTGGAAAAATGAGTGGAGATTTAAAACTTAGACCGCTTGAACGAGAAGATTTAAAATTTGTTCACCGGTTAAA
TAATGACGCGAAAATAATGT

Product: cardiolipin synthase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase 1 [H]

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MRKLIQFLFIAVVLFLVEYFLINQAAILFLVTSGLIQLCGLIIVIRLLLFDQRNTSSKVAWIAVIFILPVLGTISYLVFG
RNPATRKFSTAQVMEKAKLINAIHAIPNNTNEKLPRLSKRIAHLTSIEPIKGNKIEILTNGEETFPVLLDALRKAENHIH
IQYYIFKTDEISTKIRDILVEKAKAGVEVRFMFDGLGSSKLGKAFLAPLKEAGVSIHAFDPITSPWIVRTANLRNHRKIV
VIDGQIGFTGGLNIGEEYRSNTPDFRVWRDTHIKITGQAVIELQESFLNDWVYMENQAGAADQFISEAGLQQYFSPVDVG
DEWAQVIYGGPYDKEKWVRDSMLDLIDSAKESVWIVSPYFVPDEESLAVIRRVAMSGVDVRVIIPGKGDRGISFHGSNAY
VKTMIEAGAKMYAYADDSFVHAKAMLVDGTRAAIGTANFDVRSFRLNHELMVFLYDESEAMHHLKRDFEKDFEDSRLFTM
KDMENKPLLTRIKEVLSSLLSPIL

Sequences:

>Translated_504_residues
MRKLIQFLFIAVVLFLVEYFLINQAAILFLVTSGLIQLCGLIIVIRLLLFDQRNTSSKVAWIAVIFILPVLGTISYLVFG
RNPATRKFSTAQVMEKAKLINAIHAIPNNTNEKLPRLSKRIAHLTSIEPIKGNKIEILTNGEETFPVLLDALRKAENHIH
IQYYIFKTDEISTKIRDILVEKAKAGVEVRFMFDGLGSSKLGKAFLAPLKEAGVSIHAFDPITSPWIVRTANLRNHRKIV
VIDGQIGFTGGLNIGEEYRSNTPDFRVWRDTHIKITGQAVIELQESFLNDWVYMENQAGAADQFISEAGLQQYFSPVDVG
DEWAQVIYGGPYDKEKWVRDSMLDLIDSAKESVWIVSPYFVPDEESLAVIRRVAMSGVDVRVIIPGKGDRGISFHGSNAY
VKTMIEAGAKMYAYADDSFVHAKAMLVDGTRAAIGTANFDVRSFRLNHELMVFLYDESEAMHHLKRDFEKDFEDSRLFTM
KDMENKPLLTRIKEVLSSLLSPIL
>Mature_504_residues
MRKLIQFLFIAVVLFLVEYFLINQAAILFLVTSGLIQLCGLIIVIRLLLFDQRNTSSKVAWIAVIFILPVLGTISYLVFG
RNPATRKFSTAQVMEKAKLINAIHAIPNNTNEKLPRLSKRIAHLTSIEPIKGNKIEILTNGEETFPVLLDALRKAENHIH
IQYYIFKTDEISTKIRDILVEKAKAGVEVRFMFDGLGSSKLGKAFLAPLKEAGVSIHAFDPITSPWIVRTANLRNHRKIV
VIDGQIGFTGGLNIGEEYRSNTPDFRVWRDTHIKITGQAVIELQESFLNDWVYMENQAGAADQFISEAGLQQYFSPVDVG
DEWAQVIYGGPYDKEKWVRDSMLDLIDSAKESVWIVSPYFVPDEESLAVIRRVAMSGVDVRVIIPGKGDRGISFHGSNAY
VKTMIEAGAKMYAYADDSFVHAKAMLVDGTRAAIGTANFDVRSFRLNHELMVFLYDESEAMHHLKRDFEKDFEDSRLFTM
KDMENKPLLTRIKEVLSSLLSPIL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=512, Percent_Identity=26.7578125, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1787007, Length=328, Percent_Identity=28.0487804878049, Blast_Score=131, Evalue=8e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 56908; Mature: 56908

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLIQFLFIAVVLFLVEYFLINQAAILFLVTSGLIQLCGLIIVIRLLLFDQRNTSSKVA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
WIAVIFILPVLGTISYLVFGRNPATRKFSTAQVMEKAKLINAIHAIPNNTNEKLPRLSKR
HHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
IAHLTSIEPIKGNKIEILTNGEETFPVLLDALRKAENHIHIQYYIFKTDEISTKIRDILV
HHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEEEEECCHHHHHHHHHHH
EKAKAGVEVRFMFDGLGSSKLGKAFLAPLKEAGVSIHAFDPITSPWIVRTANLRNHRKIV
HHHHCCEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCEEEEECCCCCCCEEE
VIDGQIGFTGGLNIGEEYRSNTPDFRVWRDTHIKITGQAVIELQESFLNDWVYMENQAGA
EEECCCCCCCCCCCCHHHCCCCCCEEEEECCEEEEEHHHHHHHHHHHHCCCEEEECCCCH
ADQFISEAGLQQYFSPVDVGDEWAQVIYGGPYDKEKWVRDSMLDLIDSAKESVWIVSPYF
HHHHHHHHHHHHHCCCCCCCCHHHHHEECCCCCHHHHHHHHHHHHHHCCCCCEEEEECEE
VPDEESLAVIRRVAMSGVDVRVIIPGKGDRGISFHGSNAYVKTMIEAGAKMYAYADDSFV
CCCCHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCCCHHHHHHHHCCCEEEEECCCCEE
HAKAMLVDGTRAAIGTANFDVRSFRLNHELMVFLYDESEAMHHLKRDFEKDFEDSRLFTM
EEEEEEECCCHHEEECCCCCEEEEEECCEEEEEEECCHHHHHHHHHHHHCCCCCCEEEEE
KDMENKPLLTRIKEVLSSLLSPIL
CCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRKLIQFLFIAVVLFLVEYFLINQAAILFLVTSGLIQLCGLIIVIRLLLFDQRNTSSKVA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
WIAVIFILPVLGTISYLVFGRNPATRKFSTAQVMEKAKLINAIHAIPNNTNEKLPRLSKR
HHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
IAHLTSIEPIKGNKIEILTNGEETFPVLLDALRKAENHIHIQYYIFKTDEISTKIRDILV
HHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEEEEECCHHHHHHHHHHH
EKAKAGVEVRFMFDGLGSSKLGKAFLAPLKEAGVSIHAFDPITSPWIVRTANLRNHRKIV
HHHHCCEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCEEEEECCCCCCCEEE
VIDGQIGFTGGLNIGEEYRSNTPDFRVWRDTHIKITGQAVIELQESFLNDWVYMENQAGA
EEECCCCCCCCCCCCHHHCCCCCCEEEEECCEEEEEHHHHHHHHHHHHCCCEEEECCCCH
ADQFISEAGLQQYFSPVDVGDEWAQVIYGGPYDKEKWVRDSMLDLIDSAKESVWIVSPYF
HHHHHHHHHHHHHCCCCCCCCHHHHHEECCCCCHHHHHHHHHHHHHHCCCCCEEEEECEE
VPDEESLAVIRRVAMSGVDVRVIIPGKGDRGISFHGSNAYVKTMIEAGAKMYAYADDSFV
CCCCHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCCCHHHHHHHHCCCEEEEECCCCEE
HAKAMLVDGTRAAIGTANFDVRSFRLNHELMVFLYDESEAMHHLKRDFEKDFEDSRLFTM
EEEEEEECCCHHEEECCCCCEEEEEECCEEEEEEECCHHHHHHHHHHHHCCCCCCEEEEE
KDMENKPLLTRIKEVLSSLLSPIL
CCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]