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The map label for this gene is gyrA

Identifier: 226222646

GI number: 226222646

Start: 8065

End: 10593

Strand: Direct

Name: gyrA

Synonym: Lm4b_00007

Alternate gene names: 226222646

Gene position: 8065-10593 (Clockwise)

Preceding gene: 226222645

Following gene: 226222647

Centisome position: 0.28

GC content: 40.09

Gene sequence:

>2529_bases
ATGGCAGAAACACCAAATCAACGAATAACAGAGATAAACTTAAATAAAGAAATGCGGACATCATTCCTGGACTATGCGAT
GAGTGTAATTGTTGCCCGTGCCCTACCAGACGTTCGTGACGGATTAAAACCAGTTCATCGTCGTATTCTATATGCGATGA
ATGACTTAGGTATGACTTCTGATAAGGCCTATAAAAAATCAGCTCGTATCGTTGGTGAAGTAATCGGTAAGTATCACCCC
CACGGCGATACAGCGGTTTATTTTACAATGGTTCGGATGGCGCAAGATTTTAGTTACCGTAATATGCTAGTTGATGGACA
TGGTAACTTTGGTTCGGTTGATGGCGATATGGCGGCAGCGATGCGTTATACAGAAGCACGTATGTCAAAAATTTCGATGG
AACTTCTTCGCGATATTAATAAAGATACAATTGATTATGCTGATAACTACGATGGTTCTGAACGTGAGCCAGTCATTTTA
CCAGCGCGTTTCCCTAACTTACTTGTCAATGGTTCGTCAGGTATCGCAGTTGGTATGGCAACAAACATTCCTACCCACCA
TCTTGGTGAAGTTATCGACGGCGTTTTAGCGCTTAGTCATGATCCGGATATTACTATTCGTGATTTAATGGAATATATCC
CTGGCCCTGACTTCCCTACTGCTGGGATGATTATGGGGCGTAGCGGAATCCGTCGCGCTTACGAAAGTGGCCGTGGTTCG
ATTACTGTTCGTGGTCGTGTCGATATTGAAGAAAAGAAAAATGGTAAAGAAACAATCGTTATTACCGAAATTCCTTACCA
AGTAAATAAAGCGCGCCTAGTTGAGCGTATTGCCGAACTAGCTCGTGAGAAGAAAATCGACGGTATCACTTCCCTAAACG
ATGAATCTGACCGTTCTGGAATGCGCATTGTTATTGAAGTTCGTCGTGATATTAGTGCAAGTGTTATCGTGAATAATTTA
TTCAAAATGACAGCACTTCAAACTACTTTTGGTATTAATATGCTCGCACTTGTCGACAATCATCCAAAAGTACTTAATTT
AAAAGAAATTCTTTATCATTATTTAGAGCATCAAAAAGTAGTTATTCGTCGCCGTACGGAATTTGAGCTTCGTAAAGCAG
AAGCGCGCGCTCACATTTTAGAAGGTTTACGAATTGCACTAGATAATATTGACGCGATTATTAAATTAATTCGTGGATCA
AAAACTTCCGATGTTGCTAAAGAAGGCTTGATGACACAATTCAACCTTTCGGACAAACAAGCGCAAGCCATTTTAGACAT
GCGTTTGCAACGTTTAACAGGTTTGGAACGCGAAAAAATTGAAGAAGAATACCAAAACTTAGTGGCATTAATTAATGATT
TAAAAGCCATTTTAGCTGATGATGAGCGTATTCTTGAAATTATTCGTGAAGAATTAGAAGAAATCAAAGTTAAATACGCG
GATAAACGTCGTACAGAAATCTTGGCTGGTGATTTAGTAAGCCTTGAAGATGAAGACTTAATCCCTGAAGAAGAAGTGGC
GATTACACTAACTAAACGTGGCTATATTAAACGTTTACCATTATCAACTTATCGTAGCCAACGTCGTGGTGGTCGTGGTA
TCCAAGGTATGTCTACTCATGAAGATGATTTCGTAGAACATCTTGTTGCAACGAGCACGCATGATACGTTACTATTCTTC
ACTAACACAGGTAAAGTTTACCGTTCGAAAGGTTATGAAGTACCTGAATACGGTCGTACCGCCAAAGGTATCCCAATCAT
CAACTTACTTGGAATCGAAAGCCAAGAACAAGTGAATGCCGTGATAAATCTATCCGAATTCACTGATGATAGCTACCTAT
TCTTTACTACTAAACACGGTGTCGTGAAGCGTACAACCCTTTCTCAATTTGCGAAAATTCGTCAAAGTGGTCTTCGTGCA
GTTGAACTTCGCGAAAACGATGAACTAATCTCTGTTCAGATGACAGATGGTAGCAAAAACATGATTATCGCAACGAAACA
TGGACAATCTATCTACTTCCCAGAAGAAAATATTCGCGTAATGGGCCGTACAGCTGCTGGTGTTCGTGGTATTAGACTTC
GTGAAGACGATGAAGTTATCGGTATGGAAGTCCTTGAGGATGATGAAAAAGTTCTCGTTGTAACGGAAAAAGGATACGGT
AAACAAACACCGGCTTCCCAGTACCCGCTTCGTAATCGCGGTGGTATGGGTGTTAAAACCGTTACAATCACAGAGAAAAA
TGGTAACTTAGTCGCAATGAAAACAGTGACTGGTGAAGAAGACTTAATGCTAATGACAGTAAGTGGCGTATTAATTCGTT
TCGAAATTGATACAGTATCACAAACAGGTCGTAGCGCAATGGGTGTCAAACTAATTCGTCTTGATGAAGATGAAAAAGTA
GCTACTGTTGCAAAAGTGCCAAAAGAAGAAGATGAAGTTGAGCTTGAGGAAGAAATCGATGAAACATTAATCACACAAGT
TCCTGATGAAAGTTTTGAAGATGCTCCTGGAAGCGATATAGAAGAATAA

Upstream 100 bases:

>100_bases
GTTTAATAAATATTTCCCGGGTAATAATTGGGTTATTAATTGTTTTTAAAAGGTTTGAACTAGATTCAGCCTTACTTTTG
AAAGGGAGGTTTCTCTAACA

Downstream 100 bases:

>100_bases
AATCAAGCTGGAGATGACTCTAATTTGCGAATTAGAACGATTTTCCAGCTTTTTTCCAGTATAAAAAGCGCTTTTTGGCT
ATAATTTATGTATAATATCC

Product: DNA gyrase subunit A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 842; Mature: 841

Protein sequence:

>842_residues
MAETPNQRITEINLNKEMRTSFLDYAMSVIVARALPDVRDGLKPVHRRILYAMNDLGMTSDKAYKKSARIVGEVIGKYHP
HGDTAVYFTMVRMAQDFSYRNMLVDGHGNFGSVDGDMAAAMRYTEARMSKISMELLRDINKDTIDYADNYDGSEREPVIL
PARFPNLLVNGSSGIAVGMATNIPTHHLGEVIDGVLALSHDPDITIRDLMEYIPGPDFPTAGMIMGRSGIRRAYESGRGS
ITVRGRVDIEEKKNGKETIVITEIPYQVNKARLVERIAELAREKKIDGITSLNDESDRSGMRIVIEVRRDISASVIVNNL
FKMTALQTTFGINMLALVDNHPKVLNLKEILYHYLEHQKVVIRRRTEFELRKAEARAHILEGLRIALDNIDAIIKLIRGS
KTSDVAKEGLMTQFNLSDKQAQAILDMRLQRLTGLEREKIEEEYQNLVALINDLKAILADDERILEIIREELEEIKVKYA
DKRRTEILAGDLVSLEDEDLIPEEEVAITLTKRGYIKRLPLSTYRSQRRGGRGIQGMSTHEDDFVEHLVATSTHDTLLFF
TNTGKVYRSKGYEVPEYGRTAKGIPIINLLGIESQEQVNAVINLSEFTDDSYLFFTTKHGVVKRTTLSQFAKIRQSGLRA
VELRENDELISVQMTDGSKNMIIATKHGQSIYFPEENIRVMGRTAAGVRGIRLREDDEVIGMEVLEDDEKVLVVTEKGYG
KQTPASQYPLRNRGGMGVKTVTITEKNGNLVAMKTVTGEEDLMLMTVSGVLIRFEIDTVSQTGRSAMGVKLIRLDEDEKV
ATVAKVPKEEDEVELEEEIDETLITQVPDESFEDAPGSDIEE

Sequences:

>Translated_842_residues
MAETPNQRITEINLNKEMRTSFLDYAMSVIVARALPDVRDGLKPVHRRILYAMNDLGMTSDKAYKKSARIVGEVIGKYHP
HGDTAVYFTMVRMAQDFSYRNMLVDGHGNFGSVDGDMAAAMRYTEARMSKISMELLRDINKDTIDYADNYDGSEREPVIL
PARFPNLLVNGSSGIAVGMATNIPTHHLGEVIDGVLALSHDPDITIRDLMEYIPGPDFPTAGMIMGRSGIRRAYESGRGS
ITVRGRVDIEEKKNGKETIVITEIPYQVNKARLVERIAELAREKKIDGITSLNDESDRSGMRIVIEVRRDISASVIVNNL
FKMTALQTTFGINMLALVDNHPKVLNLKEILYHYLEHQKVVIRRRTEFELRKAEARAHILEGLRIALDNIDAIIKLIRGS
KTSDVAKEGLMTQFNLSDKQAQAILDMRLQRLTGLEREKIEEEYQNLVALINDLKAILADDERILEIIREELEEIKVKYA
DKRRTEILAGDLVSLEDEDLIPEEEVAITLTKRGYIKRLPLSTYRSQRRGGRGIQGMSTHEDDFVEHLVATSTHDTLLFF
TNTGKVYRSKGYEVPEYGRTAKGIPIINLLGIESQEQVNAVINLSEFTDDSYLFFTTKHGVVKRTTLSQFAKIRQSGLRA
VELRENDELISVQMTDGSKNMIIATKHGQSIYFPEENIRVMGRTAAGVRGIRLREDDEVIGMEVLEDDEKVLVVTEKGYG
KQTPASQYPLRNRGGMGVKTVTITEKNGNLVAMKTVTGEEDLMLMTVSGVLIRFEIDTVSQTGRSAMGVKLIRLDEDEKV
ATVAKVPKEEDEVELEEEIDETLITQVPDESFEDAPGSDIEE
>Mature_841_residues
AETPNQRITEINLNKEMRTSFLDYAMSVIVARALPDVRDGLKPVHRRILYAMNDLGMTSDKAYKKSARIVGEVIGKYHPH
GDTAVYFTMVRMAQDFSYRNMLVDGHGNFGSVDGDMAAAMRYTEARMSKISMELLRDINKDTIDYADNYDGSEREPVILP
ARFPNLLVNGSSGIAVGMATNIPTHHLGEVIDGVLALSHDPDITIRDLMEYIPGPDFPTAGMIMGRSGIRRAYESGRGSI
TVRGRVDIEEKKNGKETIVITEIPYQVNKARLVERIAELAREKKIDGITSLNDESDRSGMRIVIEVRRDISASVIVNNLF
KMTALQTTFGINMLALVDNHPKVLNLKEILYHYLEHQKVVIRRRTEFELRKAEARAHILEGLRIALDNIDAIIKLIRGSK
TSDVAKEGLMTQFNLSDKQAQAILDMRLQRLTGLEREKIEEEYQNLVALINDLKAILADDERILEIIREELEEIKVKYAD
KRRTEILAGDLVSLEDEDLIPEEEVAITLTKRGYIKRLPLSTYRSQRRGGRGIQGMSTHEDDFVEHLVATSTHDTLLFFT
NTGKVYRSKGYEVPEYGRTAKGIPIINLLGIESQEQVNAVINLSEFTDDSYLFFTTKHGVVKRTTLSQFAKIRQSGLRAV
ELRENDELISVQMTDGSKNMIIATKHGQSIYFPEENIRVMGRTAAGVRGIRLREDDEVIGMEVLEDDEKVLVVTEKGYGK
QTPASQYPLRNRGGMGVKTVTITEKNGNLVAMKTVTGEEDLMLMTVSGVLIRFEIDTVSQTGRSAMGVKLIRLDEDEKVA
TVAKVPKEEDEVELEEEIDETLITQVPDESFEDAPGSDIEE

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0188

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the topoisomerase gyrA/parC subunit family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=511, Percent_Identity=25.440313111546, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI19913406, Length=319, Percent_Identity=26.0188087774295, Blast_Score=78, Evalue=3e-14,
Organism=Escherichia coli, GI1788562, Length=843, Percent_Identity=50.1779359430605, Blast_Score=853, Evalue=0.0,
Organism=Escherichia coli, GI1789396, Length=670, Percent_Identity=37.1641791044776, Blast_Score=422, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17535065, Length=199, Percent_Identity=30.1507537688442, Blast_Score=75, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI212645845, Length=190, Percent_Identity=28.9473684210526, Blast_Score=70, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=176, Percent_Identity=30.1136363636364, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI17136538, Length=213, Percent_Identity=28.169014084507, Blast_Score=75, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020899
- InterPro:   IPR005743
- InterPro:   IPR006691
- InterPro:   IPR002205
- InterPro:   IPR013758
- InterPro:   IPR013757
- InterPro:   IPR013760 [H]

Pfam domain/function: PF03989 DNA_gyraseA_C; PF00521 DNA_topoisoIV [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 94705; Mature: 94574

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETPNQRITEINLNKEMRTSFLDYAMSVIVARALPDVRDGLKPVHRRILYAMNDLGMTS
CCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCC
DKAYKKSARIVGEVIGKYHPHGDTAVYFTMVRMAQDFSYRNMLVDGHGNFGSVDGDMAAA
CHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHH
MRYTEARMSKISMELLRDINKDTIDYADNYDGSEREPVILPARFPNLLVNGSSGIAVGMA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCEEEECCCCEEEEEE
TNIPTHHLGEVIDGVLALSHDPDITIRDLMEYIPGPDFPTAGMIMGRSGIRRAYESGRGS
CCCCHHHHHHHHHHHHEECCCCCCCHHHHHHHCCCCCCCCCCEEECCHHHHHHHHCCCCE
ITVRGRVDIEEKKNGKETIVITEIPYQVNKARLVERIAELAREKKIDGITSLNDESDRSG
EEEEEEEECHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
MRIVIEVRRDISASVIVNNLFKMTALQTTFGINMLALVDNHPKVLNLKEILYHYLEHQKV
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEECHHHHHHHHHHCCCE
VIRRRTEFELRKAEARAHILEGLRIALDNIDAIIKLIRGSKTSDVAKEGLMTQFNLSDKQ
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEECCCHHH
AQAILDMRLQRLTGLEREKIEEEYQNLVALINDLKAILADDERILEIIREELEEIKVKYA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
DKRRTEILAGDLVSLEDEDLIPEEEVAITLTKRGYIKRLPLSTYRSQRRGGRGIQGMSTH
CCHHHHHHCCCEECCCCCCCCCCCCEEEEEECCCCEEECCHHHHHHHHCCCCCCCCCCCC
EDDFVEHLVATSTHDTLLFFTNTGKVYRSKGYEVPEYGRTAKGIPIINLLGIESQEQVNA
HHHHHHHHHHCCCCCEEEEEECCCCEEECCCCCCCCCCCCCCCCCEEEEECCCCHHHHHH
VINLSEFTDDSYLFFTTKHGVVKRTTLSQFAKIRQSGLRAVELRENDELISVQMTDGSKN
EEEHHHCCCCCEEEEEECCCCEEHHHHHHHHHHHHCCCEEEEEECCCCEEEEEEECCCCC
MIIATKHGQSIYFPEENIRVMGRTAAGVRGIRLREDDEVIGMEVLEDDEKVLVVTEKGYG
EEEEEECCCEEECCCCCCEEEEECCCCCCCEEECCCCCEECHHEECCCCEEEEEEECCCC
KQTPASQYPLRNRGGMGVKTVTITEKNGNLVAMKTVTGEEDLMLMTVSGVLIRFEIDTVS
CCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEEECCCCCEEEEEECCEEEEEEECHHH
QTGRSAMGVKLIRLDEDEKVATVAKVPKEEDEVELEEEIDETLITQVPDESFEDAPGSDI
HCCCHHHCEEEEEECCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
EE
CC
>Mature Secondary Structure 
AETPNQRITEINLNKEMRTSFLDYAMSVIVARALPDVRDGLKPVHRRILYAMNDLGMTS
CCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCC
DKAYKKSARIVGEVIGKYHPHGDTAVYFTMVRMAQDFSYRNMLVDGHGNFGSVDGDMAAA
CHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHH
MRYTEARMSKISMELLRDINKDTIDYADNYDGSEREPVILPARFPNLLVNGSSGIAVGMA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCEEEECCCCEEEEEE
TNIPTHHLGEVIDGVLALSHDPDITIRDLMEYIPGPDFPTAGMIMGRSGIRRAYESGRGS
CCCCHHHHHHHHHHHHEECCCCCCCHHHHHHHCCCCCCCCCCEEECCHHHHHHHHCCCCE
ITVRGRVDIEEKKNGKETIVITEIPYQVNKARLVERIAELAREKKIDGITSLNDESDRSG
EEEEEEEECHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
MRIVIEVRRDISASVIVNNLFKMTALQTTFGINMLALVDNHPKVLNLKEILYHYLEHQKV
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEECHHHHHHHHHHCCCE
VIRRRTEFELRKAEARAHILEGLRIALDNIDAIIKLIRGSKTSDVAKEGLMTQFNLSDKQ
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEECCCHHH
AQAILDMRLQRLTGLEREKIEEEYQNLVALINDLKAILADDERILEIIREELEEIKVKYA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
DKRRTEILAGDLVSLEDEDLIPEEEVAITLTKRGYIKRLPLSTYRSQRRGGRGIQGMSTH
CCHHHHHHCCCEECCCCCCCCCCCCEEEEEECCCCEEECCHHHHHHHHCCCCCCCCCCCC
EDDFVEHLVATSTHDTLLFFTNTGKVYRSKGYEVPEYGRTAKGIPIINLLGIESQEQVNA
HHHHHHHHHHCCCCCEEEEEECCCCEEECCCCCCCCCCCCCCCCCEEEEECCCCHHHHHH
VINLSEFTDDSYLFFTTKHGVVKRTTLSQFAKIRQSGLRAVELRENDELISVQMTDGSKN
EEEHHHCCCCCEEEEEECCCCEEHHHHHHHHHHHHCCCEEEEEECCCCEEEEEEECCCCC
MIIATKHGQSIYFPEENIRVMGRTAAGVRGIRLREDDEVIGMEVLEDDEKVLVVTEKGYG
EEEEEECCCEEECCCCCCEEEEECCCCCCCEEECCCCCEECHHEECCCCEEEEEEECCCC
KQTPASQYPLRNRGGMGVKTVTITEKNGNLVAMKTVTGEEDLMLMTVSGVLIRFEIDTVS
CCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEEECCCCCEEEEEECCEEEEEEECHHH
QTGRSAMGVKLIRLDEDEKVATVAKVPKEEDEVELEEEIDETLITQVPDESFEDAPGSDI
HCCCHHHCEEEEEECCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
EE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377 [H]