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The map label for this gene is gyrB

Identifier: 226222645

GI number: 226222645

Start: 6030

End: 7970

Strand: Direct

Name: gyrB

Synonym: Lm4b_00006

Alternate gene names: 226222645

Gene position: 6030-7970 (Clockwise)

Preceding gene: 226222644

Following gene: 226222646

Centisome position: 0.21

GC content: 40.24

Gene sequence:

>1941_bases
ATGTCAGAAGAAAATATTACAAATGTACAAGAAAATGCTTCAGATTATAACGAAGATCAAATACAAGTACTGGAAGGTCT
AGAGGCAGTAAGAAAAAGACCTGGTATGTACATTGGTTCAACTAGTCAACGCGGACTCCATCACCTGGTATGGGAAATTG
TTGATAACGCAATTGATGAAGCACTTGCTGGTTTTTGTACAGAAATTGAAATTACAATCGAAGCTGATAACAGCATTACT
GTTCGTGATAACGGACGTGGAATTCCTACTGGGATTAACGAAAAAATTGGTCGTCCAACAGTAGAAGTTATCTTTACCGT
TCTACATGCTGGTGGTAAATTTGGCGGCGGCGGATATAAAGTATCTGGCGGACTTCACGGAGTTGGTGCATCGGTAGTTA
ATGCCCTTTCCACATCTCTTGAGGTATACGTTCACCGTGAAGGTCAAAAATATTACCAACGCTTTGAACGTGGTGATGTA
GTAATGGATATGGAAGAACAAGGCGAAACGGATTACCGTGGAACAATTGTTCACTTTACGCCAGATCCACAAATTTTCAC
AGAAACAACGGAATTTGATTTTGATACACTTCGTACTCGTACGCGCGAACTTGCTTTCTTGAATCGTGGTTTAACGATTT
CAATTGAAGACAAGCGCGAAGAACACAAAGTTCGTAAAGATTTCCACTATGAAGGCGGAATTCGTTCTTACGTGGAGCAT
TTAAATAAAGCAAAAGACGTTATCCATGAGCCACCAATTTATTTGGAAGGTGAACGCGATGACATTATGGTTGAGATTTC
CATGCAATATAATACTGGATTCTCAAGCAATATCATTTCATTCGCAAATAACATTCATACGTATGAAGGCGGGACTCACG
AATCTGGATTTAAAACGGCGTTAACACGTGTTATTAATGACTACGCGCGTCGTAATAAATTGTTCAAAGATAGTGATGAT
AACCTTTCCGGTGAAGATGTTCGTGAAGGTTTAACAGCAATCATTTCCATCAAACACCCAGATCCACAGTTTGAAGGACA
AACAAAAACAAAACTTGGAAATTCAGAAGCTCGTTCAATCACGGATAAGCTGTTTTCTGAGGCTTTAAATAAATTTATGA
TGGAAAACCCAGATGTTGCTAAAAAAATCGTTGAGAAGGGCGTTGTAGCTTCTCGTGCACGTCTGGCTGCTAAGCGCGCG
CGTGAAGTTGCTCGTAAAAGCAGTGGGTTAGAAATTTCTAGCTTGCCAGGTAAACTAGCTGACTGTTCTTCCCGTAACCC
TGAAATCAGCGAACTTTACATCGTTGAGGGTGACTCAGCTGGTGGTTCAGCTAAACAAGGTCGTGACCGTCTATTCCAAG
CTATTTTGCCGATTCGTGGTAAAATTTTGAACGTGGAGAAAGCGCGTTTAGACCGAATTTTAGCTAACGAAGAAATTCGA
ACTATTTTTACGGCTATGGGAACTGGTTTTGGTGGAGATTTCGATGTTTCTAAATCTCGTTACCACAAATTAATTATTAT
GACTGATGCCGATGTTGATGGTGCACATATTCGTACACTACTTCTTACGCTATTTTATCGTTATATGCGCCCACTGCTTG
ATGCTGGTTATATCTATATTGCACAACCACCGCTTTATCAAATTAAGCACGGTAAACAAATAGAATATGTATATAGCGAC
GGACAGTTAGAAGACTATCTAGCTTCCCTTGACGGAGACACTAAATACAGCATTCAACGATATAAAGGTCTTGGAGAAAT
GAACCCAGAACAACTTTGGGATACAACCATGAATCCGGAACACCGTACACTACTTCAAGTCAATATCAAAGACGCTATTG
ATGCCGATGAAACTTTTGAAATGTTGATGGGTGACCGCGTGGAACCTCGTCGTAAATTCATCGAAGACAACGCGCAATAC
GTTAAAAACTTGGATGTTTAA

Upstream 100 bases:

>100_bases
CAAACAAGCAACAACTTTCTATGTAGAAAAAGGTACGGTAAAAAAATCCTAATCTAGGTTAATATAATTTGATGAAAAAG
AAAGTGCGGTGGAATAATTA

Downstream 100 bases:

>100_bases
TAAATATTTCCCGGGTAATAATTGGGTTATTAATTGTTTTTAAAAGGTTTGAACTAGATTCAGCCTTACTTTTGAAAGGG
AGGTTTCTCTAACAATGGCA

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 646; Mature: 645

Protein sequence:

>646_residues
MSEENITNVQENASDYNEDQIQVLEGLEAVRKRPGMYIGSTSQRGLHHLVWEIVDNAIDEALAGFCTEIEITIEADNSIT
VRDNGRGIPTGINEKIGRPTVEVIFTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSTSLEVYVHREGQKYYQRFERGDV
VMDMEEQGETDYRGTIVHFTPDPQIFTETTEFDFDTLRTRTRELAFLNRGLTISIEDKREEHKVRKDFHYEGGIRSYVEH
LNKAKDVIHEPPIYLEGERDDIMVEISMQYNTGFSSNIISFANNIHTYEGGTHESGFKTALTRVINDYARRNKLFKDSDD
NLSGEDVREGLTAIISIKHPDPQFEGQTKTKLGNSEARSITDKLFSEALNKFMMENPDVAKKIVEKGVVASRARLAAKRA
REVARKSSGLEISSLPGKLADCSSRNPEISELYIVEGDSAGGSAKQGRDRLFQAILPIRGKILNVEKARLDRILANEEIR
TIFTAMGTGFGGDFDVSKSRYHKLIIMTDADVDGAHIRTLLLTLFYRYMRPLLDAGYIYIAQPPLYQIKHGKQIEYVYSD
GQLEDYLASLDGDTKYSIQRYKGLGEMNPEQLWDTTMNPEHRTLLQVNIKDAIDADETFEMLMGDRVEPRRKFIEDNAQY
VKNLDV

Sequences:

>Translated_646_residues
MSEENITNVQENASDYNEDQIQVLEGLEAVRKRPGMYIGSTSQRGLHHLVWEIVDNAIDEALAGFCTEIEITIEADNSIT
VRDNGRGIPTGINEKIGRPTVEVIFTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSTSLEVYVHREGQKYYQRFERGDV
VMDMEEQGETDYRGTIVHFTPDPQIFTETTEFDFDTLRTRTRELAFLNRGLTISIEDKREEHKVRKDFHYEGGIRSYVEH
LNKAKDVIHEPPIYLEGERDDIMVEISMQYNTGFSSNIISFANNIHTYEGGTHESGFKTALTRVINDYARRNKLFKDSDD
NLSGEDVREGLTAIISIKHPDPQFEGQTKTKLGNSEARSITDKLFSEALNKFMMENPDVAKKIVEKGVVASRARLAAKRA
REVARKSSGLEISSLPGKLADCSSRNPEISELYIVEGDSAGGSAKQGRDRLFQAILPIRGKILNVEKARLDRILANEEIR
TIFTAMGTGFGGDFDVSKSRYHKLIIMTDADVDGAHIRTLLLTLFYRYMRPLLDAGYIYIAQPPLYQIKHGKQIEYVYSD
GQLEDYLASLDGDTKYSIQRYKGLGEMNPEQLWDTTMNPEHRTLLQVNIKDAIDADETFEMLMGDRVEPRRKFIEDNAQY
VKNLDV
>Mature_645_residues
SEENITNVQENASDYNEDQIQVLEGLEAVRKRPGMYIGSTSQRGLHHLVWEIVDNAIDEALAGFCTEIEITIEADNSITV
RDNGRGIPTGINEKIGRPTVEVIFTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSTSLEVYVHREGQKYYQRFERGDVV
MDMEEQGETDYRGTIVHFTPDPQIFTETTEFDFDTLRTRTRELAFLNRGLTISIEDKREEHKVRKDFHYEGGIRSYVEHL
NKAKDVIHEPPIYLEGERDDIMVEISMQYNTGFSSNIISFANNIHTYEGGTHESGFKTALTRVINDYARRNKLFKDSDDN
LSGEDVREGLTAIISIKHPDPQFEGQTKTKLGNSEARSITDKLFSEALNKFMMENPDVAKKIVEKGVVASRARLAAKRAR
EVARKSSGLEISSLPGKLADCSSRNPEISELYIVEGDSAGGSAKQGRDRLFQAILPIRGKILNVEKARLDRILANEEIRT
IFTAMGTGFGGDFDVSKSRYHKLIIMTDADVDGAHIRTLLLTLFYRYMRPLLDAGYIYIAQPPLYQIKHGKQIEYVYSDG
QLEDYLASLDGDTKYSIQRYKGLGEMNPEQLWDTTMNPEHRTLLQVNIKDAIDADETFEMLMGDRVEPRRKFIEDNAQYV
KNLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=622, Percent_Identity=27.8135048231511, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI19913406, Length=647, Percent_Identity=26.1205564142195, Blast_Score=139, Evalue=1e-32,
Organism=Escherichia coli, GI48994957, Length=572, Percent_Identity=53.6713286713287, Blast_Score=593, Evalue=1e-171,
Organism=Escherichia coli, GI1789408, Length=631, Percent_Identity=39.4611727416799, Blast_Score=423, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17535065, Length=616, Percent_Identity=26.2987012987013, Blast_Score=138, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI212645845, Length=594, Percent_Identity=23.9057239057239, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI212645657, Length=209, Percent_Identity=27.2727272727273, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324241, Length=624, Percent_Identity=27.5641025641026, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI17136538, Length=677, Percent_Identity=25.5539143279173, Blast_Score=133, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 72815; Mature: 72684

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEENITNVQENASDYNEDQIQVLEGLEAVRKRPGMYIGSTSQRGLHHLVWEIVDNAIDE
CCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHH
ALAGFCTEIEITIEADNSITVRDNGRGIPTGINEKIGRPTVEVIFTVLHAGGKFGGGGYK
HHHCCEEEEEEEEECCCEEEEEECCCCCCCCCHHHCCCHHHHHHHHHHHCCCCCCCCCEE
VSGGLHGVGASVVNALSTSLEVYVHREGQKYYQRFERGDVVMDMEEQGETDYRGTIVHFT
ECCCCCHHHHHHHHHHHHCEEEEEEECCHHHHHHHHCCCEEEEHHHCCCCCCCCEEEEEC
PDPQIFTETTEFDFDTLRTRTRELAFLNRGLTISIEDKREEHKVRKDFHYEGGIRSYVEH
CCCCEEECCCCCCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCCCHHHHHHH
LNKAKDVIHEPPIYLEGERDDIMVEISMQYNTGFSSNIISFANNIHTYEGGTHESGFKTA
HHHHHHHHCCCCEEEECCCCCEEEEEEEEECCCCCHHHHHHHHCCEECCCCCCCCHHHHH
LTRVINDYARRNKLFKDSDDNLSGEDVREGLTAIISIKHPDPQFEGQTKTKLGNSEARSI
HHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCEEEEEECCCCCCCCCCHHHCCCCHHHHHH
TDKLFSEALNKFMMENPDVAKKIVEKGVVASRARLAAKRAREVARKSSGLEISSLPGKLA
HHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHH
DCSSRNPEISELYIVEGDSAGGSAKQGRDRLFQAILPIRGKILNVEKARLDRILANEEIR
CCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHH
TIFTAMGTGFGGDFDVSKSRYHKLIIMTDADVDGAHIRTLLLTLFYRYMRPLLDAGYIYI
HHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEE
AQPPLYQIKHGKQIEYVYSDGQLEDYLASLDGDTKYSIQRYKGLGEMNPEQLWDTTMNPE
ECCCCCCCCCCCEEEEEECCCCHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHCCCCCCC
HRTLLQVNIKDAIDADETFEMLMGDRVEPRRKFIEDNAQYVKNLDV
CCEEEEEECHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SEENITNVQENASDYNEDQIQVLEGLEAVRKRPGMYIGSTSQRGLHHLVWEIVDNAIDE
CCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHH
ALAGFCTEIEITIEADNSITVRDNGRGIPTGINEKIGRPTVEVIFTVLHAGGKFGGGGYK
HHHCCEEEEEEEEECCCEEEEEECCCCCCCCCHHHCCCHHHHHHHHHHHCCCCCCCCCEE
VSGGLHGVGASVVNALSTSLEVYVHREGQKYYQRFERGDVVMDMEEQGETDYRGTIVHFT
ECCCCCHHHHHHHHHHHHCEEEEEEECCHHHHHHHHCCCEEEEHHHCCCCCCCCEEEEEC
PDPQIFTETTEFDFDTLRTRTRELAFLNRGLTISIEDKREEHKVRKDFHYEGGIRSYVEH
CCCCEEECCCCCCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCCCHHHHHHH
LNKAKDVIHEPPIYLEGERDDIMVEISMQYNTGFSSNIISFANNIHTYEGGTHESGFKTA
HHHHHHHHCCCCEEEECCCCCEEEEEEEEECCCCCHHHHHHHHCCEECCCCCCCCHHHHH
LTRVINDYARRNKLFKDSDDNLSGEDVREGLTAIISIKHPDPQFEGQTKTKLGNSEARSI
HHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCEEEEEECCCCCCCCCCHHHCCCCHHHHHH
TDKLFSEALNKFMMENPDVAKKIVEKGVVASRARLAAKRAREVARKSSGLEISSLPGKLA
HHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHH
DCSSRNPEISELYIVEGDSAGGSAKQGRDRLFQAILPIRGKILNVEKARLDRILANEEIR
CCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHH
TIFTAMGTGFGGDFDVSKSRYHKLIIMTDADVDGAHIRTLLLTLFYRYMRPLLDAGYIYI
HHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEE
AQPPLYQIKHGKQIEYVYSDGQLEDYLASLDGDTKYSIQRYKGLGEMNPEQLWDTTMNPE
ECCCCCCCCCCCEEEEEECCCCHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHCCCCCCC
HRTLLQVNIKDAIDADETFEMLMGDRVEPRRKFIEDNAQYVKNLDV
CCEEEEEECHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377 [H]