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The map label for this gene is recF

Identifier: 226222644

GI number: 226222644

Start: 4869

End: 5981

Strand: Direct

Name: recF

Synonym: Lm4b_00005

Alternate gene names: 226222644

Gene position: 4869-5981 (Clockwise)

Preceding gene: 226222643

Following gene: 226222645

Centisome position: 0.17

GC content: 39.17

Gene sequence:

>1113_bases
ATGCATTTAGAAAGTATTGTTTTAAGAAATTTCCGGAATTACGAAAACTTAGAACTAGAATTTTCCCCATCTGTTAATGT
TTTTCTTGGTGAGAATGCACAAGGTAAAACCAACCTTTTAGAAGCCGTATTGATGCTTGCACTTGCAAAGTCACATCGGA
CAACGAATGATAAAGATTTTATTATGTGGGAAAAAGAAGAAGCCAAGATGGAAGGGCGAATAGCAAAGCACGGACAATCA
GTGCCTTTAGAGCTTGCTATCACCCAAAAAGGTAAGCGAGCTAAAGTAAATCATTTGGAACAAAAGAAACTTAGCCAATA
TGTCGGTAACTTGAATGTGGTTATTTTTGCCCCAGAAGATTTATCGCTTGTGAAGGGCGCTCCAGGAATTAGACGTCGGT
TTTTGAATATGGAAATTGGACAAATGCAGCCGATTTATTTGCATAATCTAAGTGAATACCAACGAATATTGCAACAACGG
AACCAATATTTGAAGATGTTGCAAATGAAACGAAAAGTAGATCCAATATTACTCGACATTTTGACTGAACAGTTCGCAGA
TGTCGCCATTAATTTGACGAAAAGACGAGCTGATTTCATTCAAAAACTAGAGGCATATGCGGCGCCAATTCATCACCAAA
TTTCCCGAGGACTTGAGACGCTGAAAATTGAGTATAAAGCTTCCATCACGCTAAATGGGGACGATCCCGAAGTGTGGAAA
GCAGATTTGCTCCAAAAAATGGAATCAATCAAACAAAGAGAAATCGACCGTGGTGTCACGCTTATTGGACCACATCGGGA
TGATTCTCTGTTTTATATTAATGGGCAAAATGTGCAAGATTTTGGTTCGCAAGGTCAGCAACGGACAACGGCACTTTCTA
TAAAATTAGCAGAAATTGACCTAATCCATGAAGAAACTGGCGAATATCCGGTTCTTCTTCTCGATGATGTGCTTAGTGAA
CTCGATGATTATCGTCAATCGCATTTGCTCGGAGCTATTGAAGGAAAAGTACAAACCTTTGTAACGACAACAAGTACGAG
CGGGATCGACCATGAAACGCTCAAACAAGCAACAACTTTCTATGTAGAAAAAGGTACGGTAAAAAAATCCTAA

Upstream 100 bases:

>100_bases
CGGAGAGCAAGACAACCGCCGGGGGAAAAAGCTTCGTAATGGCGATGTTATCTTAGTTCCCGGTGTTGGAAAAGTGAAAA
TCGAGCAAGGAAAATAACAG

Downstream 100 bases:

>100_bases
TCTAGGTTAATATAATTTGATGAAAAAGAAAGTGCGGTGGAATAATTAATGTCAGAAGAAAATATTACAAATGTACAAGA
AAATGCTTCAGATTATAACG

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MHLESIVLRNFRNYENLELEFSPSVNVFLGENAQGKTNLLEAVLMLALAKSHRTTNDKDFIMWEKEEAKMEGRIAKHGQS
VPLELAITQKGKRAKVNHLEQKKLSQYVGNLNVVIFAPEDLSLVKGAPGIRRRFLNMEIGQMQPIYLHNLSEYQRILQQR
NQYLKMLQMKRKVDPILLDILTEQFADVAINLTKRRADFIQKLEAYAAPIHHQISRGLETLKIEYKASITLNGDDPEVWK
ADLLQKMESIKQREIDRGVTLIGPHRDDSLFYINGQNVQDFGSQGQQRTTALSIKLAEIDLIHEETGEYPVLLLDDVLSE
LDDYRQSHLLGAIEGKVQTFVTTTSTSGIDHETLKQATTFYVEKGTVKKS

Sequences:

>Translated_370_residues
MHLESIVLRNFRNYENLELEFSPSVNVFLGENAQGKTNLLEAVLMLALAKSHRTTNDKDFIMWEKEEAKMEGRIAKHGQS
VPLELAITQKGKRAKVNHLEQKKLSQYVGNLNVVIFAPEDLSLVKGAPGIRRRFLNMEIGQMQPIYLHNLSEYQRILQQR
NQYLKMLQMKRKVDPILLDILTEQFADVAINLTKRRADFIQKLEAYAAPIHHQISRGLETLKIEYKASITLNGDDPEVWK
ADLLQKMESIKQREIDRGVTLIGPHRDDSLFYINGQNVQDFGSQGQQRTTALSIKLAEIDLIHEETGEYPVLLLDDVLSE
LDDYRQSHLLGAIEGKVQTFVTTTSTSGIDHETLKQATTFYVEKGTVKKS
>Mature_370_residues
MHLESIVLRNFRNYENLELEFSPSVNVFLGENAQGKTNLLEAVLMLALAKSHRTTNDKDFIMWEKEEAKMEGRIAKHGQS
VPLELAITQKGKRAKVNHLEQKKLSQYVGNLNVVIFAPEDLSLVKGAPGIRRRFLNMEIGQMQPIYLHNLSEYQRILQQR
NQYLKMLQMKRKVDPILLDILTEQFADVAINLTKRRADFIQKLEAYAAPIHHQISRGLETLKIEYKASITLNGDDPEVWK
ADLLQKMESIKQREIDRGVTLIGPHRDDSLFYINGQNVQDFGSQGQQRTTALSIKLAEIDLIHEETGEYPVLLLDDVLSE
LDDYRQSHLLGAIEGKVQTFVTTTSTSGIDHETLKQATTFYVEKGTVKKS

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=372, Percent_Identity=25.5376344086022, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 42256; Mature: 42256

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS00617 RECF_1 ; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLESIVLRNFRNYENLELEFSPSVNVFLGENAQGKTNLLEAVLMLALAKSHRTTNDKDF
CCHHHHHHHHCCCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCE
IMWEKEEAKMEGRIAKHGQSVPLELAITQKGKRAKVNHLEQKKLSQYVGNLNVVIFAPED
EEEEHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCC
LSLVKGAPGIRRRFLNMEIGQMQPIYLHNLSEYQRILQQRNQYLKMLQMKRKVDPILLDI
CHHHCCCCHHHHHHHCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LTEQFADVAINLTKRRADFIQKLEAYAAPIHHQISRGLETLKIEYKASITLNGDDPEVWK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEEEEEEEECCCCCHHHH
ADLLQKMESIKQREIDRGVTLIGPHRDDSLFYINGQNVQDFGSQGQQRTTALSIKLAEID
HHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEECCCHHHHHCCCCCCEEEEEEEEEHEE
LIHEETGEYPVLLLDDVLSELDDYRQSHLLGAIEGKVQTFVTTTSTSGIDHETLKQATTF
EEHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHE
YVEKGTVKKS
EEECCCCCCC
>Mature Secondary Structure
MHLESIVLRNFRNYENLELEFSPSVNVFLGENAQGKTNLLEAVLMLALAKSHRTTNDKDF
CCHHHHHHHHCCCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCE
IMWEKEEAKMEGRIAKHGQSVPLELAITQKGKRAKVNHLEQKKLSQYVGNLNVVIFAPED
EEEEHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCC
LSLVKGAPGIRRRFLNMEIGQMQPIYLHNLSEYQRILQQRNQYLKMLQMKRKVDPILLDI
CHHHCCCCHHHHHHHCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LTEQFADVAINLTKRRADFIQKLEAYAAPIHHQISRGLETLKIEYKASITLNGDDPEVWK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEEEEEEEECCCCCHHHH
ADLLQKMESIKQREIDRGVTLIGPHRDDSLFYINGQNVQDFGSQGQQRTTALSIKLAEID
HHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEECCCHHHHHCCCCCCEEEEEEEEEHEE
LIHEETGEYPVLLLDDVLSELDDYRQSHLLGAIEGKVQTFVTTTSTSGIDHETLKQATTF
EEHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHE
YVEKGTVKKS
EEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]