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The map label for this gene is entE [H]

Identifier: 225685883

GI number: 225685883

Start: 12098

End: 13717

Strand: Reverse

Name: entE [H]

Synonym: BMEA_B0017

Alternate gene names: 225685883

Gene position: 13717-12098 (Counterclockwise)

Preceding gene: 225685884

Following gene: 225685882

Centisome position: 1.16

GC content: 61.05

Gene sequence:

>1620_bases
ATGACCATCGAATTTACCCATTGGCCACCGGCACGCGAACGCCTCTATCGCGAAAAGGGCTACTGGATCGACAAACCCCT
CACCCGCGCACTGGAAGAACAGGCCGCCATGCGGCCCGATGCGCCCGCTATCCTCTGCGGCGAGCGGCGTTTCACCTATG
CCGAACTCGACCGTCTGTCTTCCAACCTGGCCTCCCGGCTTGCGGCAGCCGGCATAGGCAAAGGCGACACGGCACTGGTG
CAACTGCCCAATATCGCCGAATTCTACATCGTGTTCTTCGCACTCATGAAAGCGGGCATCGCGCCCGTCAATGCGCTTTT
CAGCCATCGCAAGCTGGAGCTTGGCGCCTATGCCAGCCAGATCGAACCACGGCTGCTGATCGGCTCACGCCAGCATGAAC
TCTTCATGGATGACGCCTTTGCCCGCGACCTTGGCAAAAATCTTTCCGCACCGCTGCTAACACTTTTTGCAGGCGAGGCC
GACCCTGCGTCGAGCCTCGACCACTGGATCGCCACGCCTGCGGACAAAGCCGTTCCCTTCTCGCCAACCGGCGCTGGCGA
AGTCGCCTTTTTCCAGCTTTCCGGCGGCAGCACCGGCACGCCGAAACTCATCCCGCGCACGCATAATGATTATGATTACA
GCGTGCGCGCCAGCGCGGAAATCTGCGCGCTGACACCGCAGACCCGTTTTCTCTGCGCCCTGCCCACCGCGCATAATTAT
CCAATGAGTTCGCCCGGCGCGCTCGGCGTTTTCCATGCGGGCGGCTGTGTCGTCATGGCCCCCAACCCGGAACCGCTCAA
CTGCTTCTCCATCATCGAACGTCATGGCGTAAACATGGTGGCACTGGTGCCTCCGGCAGTGGCGCTCTGGCTACAGGCAG
CCCCCGATCATCTGGCAGCACTCAGCTCGCTGAAACTCGTGCAGGTGGGTGGCGCAAGCTTTGCGGAGGCGCTTGCCCGG
CAGGTGCCACAGGTTCTGGGCTGCAAGCTGCAACAGGTCTTCGGCATGGCCGAGGGGCTGGTCAACTATACCCGCCTTGA
CGACCCGGACGAGATCGTCTTCACCACCCAGGGCCGTCCGATCAGCCCGGACGATGAAATCAGGATCGTCGATGAGGATG
GCGAACCCGTGGCCGAAGGCCAGCCAGGAATGCTGGCCACGCGCGGGCCTTACACGTTCTGCGGCTATTATCGCAGCCCC
GAACATAATGCGCAGGTCTTCGACAGTGAAGGCTTTTATTATTCGGGCGACGTGGTCCAGCGCACGCCGGAAGGCTATCT
GCGCGTCGTGGGCCGCGTGAAGGACCAGATTAATCGCGGCGGCGAGAAAGTTGCCGCCGAGGAGATTGAAAACCTAATCC
TCCTCCATCCCGATGTGACGCACGCCGCACTTGTCGCCATGCAGGATGAATTGCTGGGCGAGAAAAGCTGCGCTTTCATC
GTCTCGCGCAATCCCGATCTCAAGCCGCCTGTTCTGCGGCGTCACCTGCTGGCGCTGGGCGTTGCCGAATACAAGCTTCC
AGACCGCATCAGGCTCATTGAAACCATGCCGCTCACCGCAGTTGGCAAGATCGACAAAAAGCATCTGCGCAAGCTTTCAA
GCCCATCCTCCCCCGGTTAG

Upstream 100 bases:

>100_bases
GCCTCTCCATGTCTTTGTTTCTACGCATTATCCGACGCAGAGCCGCTTCGCATTGCGGGCTCGAAAACTGCACTCGTTTT
TATTTCAAGCAGAACAAGTC

Downstream 100 bases:

>100_bases
CTCGCGTTTTACTCCAGTCGGATTACGCTTCAAACCCACAAAGGCAAGACCATGGCCATTCCCAAAATCGCCGATTATCC
AATGCCGCAAAAGGCAGGCT

Product: enterobactin synthase subunit E

Products: NA

Alternate protein names: Enterochelin synthase E; 2,3-dihydroxybenzoate-AMP ligase; Dihydroxybenzoic acid-activating enzyme; S-dihydroxybenzoyltransferase [H]

Number of amino acids: Translated: 539; Mature: 538

Protein sequence:

>539_residues
MTIEFTHWPPARERLYREKGYWIDKPLTRALEEQAAMRPDAPAILCGERRFTYAELDRLSSNLASRLAAAGIGKGDTALV
QLPNIAEFYIVFFALMKAGIAPVNALFSHRKLELGAYASQIEPRLLIGSRQHELFMDDAFARDLGKNLSAPLLTLFAGEA
DPASSLDHWIATPADKAVPFSPTGAGEVAFFQLSGGSTGTPKLIPRTHNDYDYSVRASAEICALTPQTRFLCALPTAHNY
PMSSPGALGVFHAGGCVVMAPNPEPLNCFSIIERHGVNMVALVPPAVALWLQAAPDHLAALSSLKLVQVGGASFAEALAR
QVPQVLGCKLQQVFGMAEGLVNYTRLDDPDEIVFTTQGRPISPDDEIRIVDEDGEPVAEGQPGMLATRGPYTFCGYYRSP
EHNAQVFDSEGFYYSGDVVQRTPEGYLRVVGRVKDQINRGGEKVAAEEIENLILLHPDVTHAALVAMQDELLGEKSCAFI
VSRNPDLKPPVLRRHLLALGVAEYKLPDRIRLIETMPLTAVGKIDKKHLRKLSSPSSPG

Sequences:

>Translated_539_residues
MTIEFTHWPPARERLYREKGYWIDKPLTRALEEQAAMRPDAPAILCGERRFTYAELDRLSSNLASRLAAAGIGKGDTALV
QLPNIAEFYIVFFALMKAGIAPVNALFSHRKLELGAYASQIEPRLLIGSRQHELFMDDAFARDLGKNLSAPLLTLFAGEA
DPASSLDHWIATPADKAVPFSPTGAGEVAFFQLSGGSTGTPKLIPRTHNDYDYSVRASAEICALTPQTRFLCALPTAHNY
PMSSPGALGVFHAGGCVVMAPNPEPLNCFSIIERHGVNMVALVPPAVALWLQAAPDHLAALSSLKLVQVGGASFAEALAR
QVPQVLGCKLQQVFGMAEGLVNYTRLDDPDEIVFTTQGRPISPDDEIRIVDEDGEPVAEGQPGMLATRGPYTFCGYYRSP
EHNAQVFDSEGFYYSGDVVQRTPEGYLRVVGRVKDQINRGGEKVAAEEIENLILLHPDVTHAALVAMQDELLGEKSCAFI
VSRNPDLKPPVLRRHLLALGVAEYKLPDRIRLIETMPLTAVGKIDKKHLRKLSSPSSPG
>Mature_538_residues
TIEFTHWPPARERLYREKGYWIDKPLTRALEEQAAMRPDAPAILCGERRFTYAELDRLSSNLASRLAAAGIGKGDTALVQ
LPNIAEFYIVFFALMKAGIAPVNALFSHRKLELGAYASQIEPRLLIGSRQHELFMDDAFARDLGKNLSAPLLTLFAGEAD
PASSLDHWIATPADKAVPFSPTGAGEVAFFQLSGGSTGTPKLIPRTHNDYDYSVRASAEICALTPQTRFLCALPTAHNYP
MSSPGALGVFHAGGCVVMAPNPEPLNCFSIIERHGVNMVALVPPAVALWLQAAPDHLAALSSLKLVQVGGASFAEALARQ
VPQVLGCKLQQVFGMAEGLVNYTRLDDPDEIVFTTQGRPISPDDEIRIVDEDGEPVAEGQPGMLATRGPYTFCGYYRSPE
HNAQVFDSEGFYYSGDVVQRTPEGYLRVVGRVKDQINRGGEKVAAEEIENLILLHPDVTHAALVAMQDELLGEKSCAFIV
SRNPDLKPPVLRRHLLALGVAEYKLPDRIRLIETMPLTAVGKIDKKHLRKLSSPSSPG

Specific function: Activates the carboxylate group of 2,3-dihydroxy- benzoate (2,3-DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate. Then, catalyzes the acylation of holo-EntB with 2,3-DHB adenylate, preparing that molecule for amide bond formation with

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. EntE subfamily [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=543, Percent_Identity=23.0202578268877, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI38505220, Length=524, Percent_Identity=23.2824427480916, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI42544132, Length=542, Percent_Identity=22.8782287822878, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI58082049, Length=538, Percent_Identity=23.7918215613383, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI157311624, Length=533, Percent_Identity=23.4521575984991, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI157311622, Length=533, Percent_Identity=23.4521575984991, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI122937307, Length=534, Percent_Identity=24.3445692883895, Blast_Score=100, Evalue=7e-21,
Organism=Homo sapiens, GI115511026, Length=539, Percent_Identity=24.6753246753247, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI187761345, Length=544, Percent_Identity=24.8161764705882, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI187761343, Length=544, Percent_Identity=24.8161764705882, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI28416953, Length=532, Percent_Identity=25.187969924812, Blast_Score=94, Evalue=5e-19,
Organism=Escherichia coli, GI1786810, Length=536, Percent_Identity=57.8358208955224, Blast_Score=612, Evalue=1e-176,
Organism=Escherichia coli, GI145693145, Length=546, Percent_Identity=32.0512820512821, Blast_Score=225, Evalue=6e-60,
Organism=Escherichia coli, GI1788107, Length=540, Percent_Identity=24.8148148148148, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI221142682, Length=490, Percent_Identity=24.0816326530612, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1790505, Length=534, Percent_Identity=22.2846441947566, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786529, Length=563, Percent_Identity=23.0905861456483, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1786801, Length=462, Percent_Identity=25.5411255411255, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1789201, Length=355, Percent_Identity=25.3521126760563, Blast_Score=78, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17557194, Length=544, Percent_Identity=27.5735294117647, Blast_Score=128, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17538037, Length=540, Percent_Identity=25.9259259259259, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17531443, Length=539, Percent_Identity=23.0055658627087, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17560308, Length=528, Percent_Identity=25.3787878787879, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17559526, Length=562, Percent_Identity=25.0889679715303, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI32563687, Length=511, Percent_Identity=22.1135029354207, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71994703, Length=528, Percent_Identity=24.0530303030303, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71994694, Length=528, Percent_Identity=24.0530303030303, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71994690, Length=528, Percent_Identity=24.0530303030303, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17558820, Length=520, Percent_Identity=21.7307692307692, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17560140, Length=286, Percent_Identity=25.8741258741259, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71985884, Length=398, Percent_Identity=23.1155778894472, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71983001, Length=385, Percent_Identity=24.9350649350649, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71982997, Length=385, Percent_Identity=24.9350649350649, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=359, Percent_Identity=24.5125348189415, Blast_Score=114, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6323182, Length=606, Percent_Identity=23.4323432343234, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6319264, Length=541, Percent_Identity=24.2144177449168, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24581924, Length=534, Percent_Identity=23.9700374531835, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI18859661, Length=510, Percent_Identity=24.1176470588235, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI19922652, Length=504, Percent_Identity=22.2222222222222, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21358303, Length=298, Percent_Identity=24.8322147651007, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI281365686, Length=299, Percent_Identity=26.7558528428094, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24648260, Length=275, Percent_Identity=24.3636363636364, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI21356947, Length=399, Percent_Identity=23.5588972431078, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21355181, Length=498, Percent_Identity=22.8915662650602, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24653035, Length=288, Percent_Identity=25, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24656500, Length=498, Percent_Identity=23.6947791164659, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24666501, Length=359, Percent_Identity=26.7409470752089, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24666497, Length=359, Percent_Identity=26.7409470752089, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24667955, Length=573, Percent_Identity=23.0366492146597, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI62472339, Length=547, Percent_Identity=23.400365630713, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281366413, Length=359, Percent_Identity=26.7409470752089, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI161076582, Length=245, Percent_Identity=26.1224489795918, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24648257, Length=273, Percent_Identity=24.1758241758242, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24667959, Length=379, Percent_Identity=24.5382585751979, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =2.7.7.58 [H]

Molecular weight: Translated: 58757; Mature: 58626

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00605 ATPASE_C ; PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIEFTHWPPARERLYREKGYWIDKPLTRALEEQAAMRPDAPAILCGERRFTYAELDRLS
CEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHH
SNLASRLAAAGIGKGDTALVQLPNIAEFYIVFFALMKAGIAPVNALFSHRKLELGAYASQ
HHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHCCCEEHHHHHHH
IEPRLLIGSRQHELFMDDAFARDLGKNLSAPLLTLFAGEADPASSLDHWIATPADKAVPF
CCCEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCC
SPTGAGEVAFFQLSGGSTGTPKLIPRTHNDYDYSVRASAEICALTPQTRFLCALPTAHNY
CCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCCCEEEECCCCCCC
PMSSPGALGVFHAGGCVVMAPNPEPLNCFSIIERHGVNMVALVPPAVALWLQAAPDHLAA
CCCCCCCEEEEECCCEEEECCCCCCCHHHHHHHHCCCCEEEECCHHHHHHHHCCCHHHHH
LSSLKLVQVGGASFAEALARQVPQVLGCKLQQVFGMAEGLVNYTRLDDPDEIVFTTQGRP
HHHCEEEEECCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCC
ISPDDEIRIVDEDGEPVAEGQPGMLATRGPYTFCGYYRSPEHNAQVFDSEGFYYSGDVVQ
CCCCCCEEEECCCCCCCCCCCCCEEEECCCCEEEECCCCCCCCCEEEECCCCEEECCHHC
RTPEGYLRVVGRVKDQINRGGEKVAAEEIENLILLHPDVTHAALVAMQDELLGEKSCAFI
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCEEEECCCHHHHHHHHHHHHHCCCCCEEEE
VSRNPDLKPPVLRRHLLALGVAEYKLPDRIRLIETMPLTAVGKIDKKHLRKLSSPSSPG
EECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure 
TIEFTHWPPARERLYREKGYWIDKPLTRALEEQAAMRPDAPAILCGERRFTYAELDRLS
EEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHH
SNLASRLAAAGIGKGDTALVQLPNIAEFYIVFFALMKAGIAPVNALFSHRKLELGAYASQ
HHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHCCCEEHHHHHHH
IEPRLLIGSRQHELFMDDAFARDLGKNLSAPLLTLFAGEADPASSLDHWIATPADKAVPF
CCCEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCC
SPTGAGEVAFFQLSGGSTGTPKLIPRTHNDYDYSVRASAEICALTPQTRFLCALPTAHNY
CCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCCCEEEECCCCCCC
PMSSPGALGVFHAGGCVVMAPNPEPLNCFSIIERHGVNMVALVPPAVALWLQAAPDHLAA
CCCCCCCEEEEECCCEEEECCCCCCCHHHHHHHHCCCCEEEECCHHHHHHHHCCCHHHHH
LSSLKLVQVGGASFAEALARQVPQVLGCKLQQVFGMAEGLVNYTRLDDPDEIVFTTQGRP
HHHCEEEEECCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCC
ISPDDEIRIVDEDGEPVAEGQPGMLATRGPYTFCGYYRSPEHNAQVFDSEGFYYSGDVVQ
CCCCCCEEEECCCCCCCCCCCCCEEEECCCCEEEECCCCCCCCCEEEECCCCEEECCHHC
RTPEGYLRVVGRVKDQINRGGEKVAAEEIENLILLHPDVTHAALVAMQDELLGEKSCAFI
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCEEEECCCHHHHHHHHHHHHHCCCCCEEEE
VSRNPDLKPPVLRRHLLALGVAEYKLPDRIRLIETMPLTAVGKIDKKHLRKLSSPSSPG
EECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]