Click here to switch to the map view.

The map label for this gene is entB [H]

Identifier: 225685882

GI number: 225685882

Start: 11174

End: 12046

Strand: Reverse

Name: entB [H]

Synonym: BMEA_B0016

Alternate gene names: 225685882

Gene position: 12046-11174 (Counterclockwise)

Preceding gene: 225685883

Following gene: 225685881

Centisome position: 1.02

GC content: 60.71

Gene sequence:

>873_bases
ATGGCCATTCCCAAAATCGCCGATTATCCAATGCCGCAAAAGGCAGGCTTTCCCGCCAACAAGACGAACTGGCAACCCGA
TCCCGCCCGCGCGGTGCTGCTGATCCACGACATGCAGCGCTATTTCCTGCGCTTTTACGACGCAAACGGCCCGCTGATGG
GCGATCTTATCGGCAATCTCGTTCGCCTGCGCAACTGGGCGCATGAAAACGGCGTGCCGGTGGTCTATACGGCGCAGCCG
CACGAGCAGCCAGCAGGCTACCGCGCCCTTCTCAACGATATGTGGGGGCCGGGCTTGCCCGCCGCCGCGCCGGAACAGCA
GCTCATCGTGGACAAACTGACGCCCGGCGCCACCGATACGGTGCTGGCCAAATGGCGCTACAGCGCCTTCCAGCGGTCCG
ACCTTCTGCAACGCATGAAAGACTGGAACCGCGACCAATTGATTATCGGCGGCATCTACGCCCATATCGGTTGCATGGTG
ACGGCCATCGATGCCTTCATGAACGACATCCAGCCTTTCATGGTGGGCGATGCGGTTGCCGACTTCTCCGAGGAAGAACA
TCGCCTTGCGCTGAAATATGTCGCGACCCGCTGCGGCGCGGTCATCGACACGGCCAGCCTTATCAGAAAGCCCGCCACTG
AAACCACCCGCGACTGGCTGCAAAAGCGGGTTCTGGAACTGATCGAGGATGAGACGGAACTCGACCCGCAGGAAAACCTC
ATCCTCTACGGCCTCGATTCGCTACAGGTGATGAAGCTTTCCGCCGAACTGAAAGAGCGCGGCGTGGCCGTGGGCTTTGA
TGATCTTGCCCGCGTGCCAACCCTTGCGGCCTGGTGGGACCTGATCGAAACAAAGCAGAAGGCAGCCGCCTGA

Upstream 100 bases:

>100_bases
CGACAAAAAGCATCTGCGCAAGCTTTCAAGCCCATCCTCCCCCGGTTAGCTCGCGTTTTACTCCAGTCGGATTACGCTTC
AAACCCACAAAGGCAAGACC

Downstream 100 bases:

>100_bases
TGGTCGAGGCAAAATCCTATCCGGCAAGCTGGCGCTTTGCCGGAAAACGGGTGATCGTCACCGGCGCCGCACAAGGCATC
GGCCGCCGTGTGGCGGAGCA

Product: putative isochorismatase

Products: NA

Alternate protein names: 2,3 dihydro-2,3 dihydroxybenzoate synthase; Enterobactin synthase component B; Enterochelin synthase B; Isochorismate lyase [H]

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MAIPKIADYPMPQKAGFPANKTNWQPDPARAVLLIHDMQRYFLRFYDANGPLMGDLIGNLVRLRNWAHENGVPVVYTAQP
HEQPAGYRALLNDMWGPGLPAAAPEQQLIVDKLTPGATDTVLAKWRYSAFQRSDLLQRMKDWNRDQLIIGGIYAHIGCMV
TAIDAFMNDIQPFMVGDAVADFSEEEHRLALKYVATRCGAVIDTASLIRKPATETTRDWLQKRVLELIEDETELDPQENL
ILYGLDSLQVMKLSAELKERGVAVGFDDLARVPTLAAWWDLIETKQKAAA

Sequences:

>Translated_290_residues
MAIPKIADYPMPQKAGFPANKTNWQPDPARAVLLIHDMQRYFLRFYDANGPLMGDLIGNLVRLRNWAHENGVPVVYTAQP
HEQPAGYRALLNDMWGPGLPAAAPEQQLIVDKLTPGATDTVLAKWRYSAFQRSDLLQRMKDWNRDQLIIGGIYAHIGCMV
TAIDAFMNDIQPFMVGDAVADFSEEEHRLALKYVATRCGAVIDTASLIRKPATETTRDWLQKRVLELIEDETELDPQENL
ILYGLDSLQVMKLSAELKERGVAVGFDDLARVPTLAAWWDLIETKQKAAA
>Mature_289_residues
AIPKIADYPMPQKAGFPANKTNWQPDPARAVLLIHDMQRYFLRFYDANGPLMGDLIGNLVRLRNWAHENGVPVVYTAQPH
EQPAGYRALLNDMWGPGLPAAAPEQQLIVDKLTPGATDTVLAKWRYSAFQRSDLLQRMKDWNRDQLIIGGIYAHIGCMVT
AIDAFMNDIQPFMVGDAVADFSEEEHRLALKYVATRCGAVIDTASLIRKPATETTRDWLQKRVLELIEDETELDPQENLI
LYGLDSLQVMKLSAELKERGVAVGFDDLARVPTLAAWWDLIETKQKAAA

Specific function: Required for production of 2,3-DHB. Also serves as an aryl carrier protein and plays a role in enterobactin assembly [H]

COG id: COG1535

COG function: function code Q; Isochorismate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 acyl carrier domain [H]

Homologues:

Organism=Escherichia coli, GI1786811, Length=287, Percent_Identity=51.219512195122, Blast_Score=291, Evalue=5e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009081
- InterPro:   IPR016291
- InterPro:   IPR000868
- InterPro:   IPR006163 [H]

Pfam domain/function: PF00857 Isochorismatase; PF00550 PP-binding [H]

EC number: =3.3.2.1 [H]

Molecular weight: Translated: 32587; Mature: 32456

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS50075 ACP_DOMAIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIPKIADYPMPQKAGFPANKTNWQPDPARAVLLIHDMQRYFLRFYDANGPLMGDLIGNL
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VRLRNWAHENGVPVVYTAQPHEQPAGYRALLNDMWGPGLPAAAPEQQLIVDKLTPGATDT
HHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCHH
VLAKWRYSAFQRSDLLQRMKDWNRDQLIIGGIYAHIGCMVTAIDAFMNDIQPFMVGDAVA
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
DFSEEEHRLALKYVATRCGAVIDTASLIRKPATETTRDWLQKRVLELIEDETELDPQENL
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHCCCCCCCE
ILYGLDSLQVMKLSAELKERGVAVGFDDLARVPTLAAWWDLIETKQKAAA
EEEECCCHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AIPKIADYPMPQKAGFPANKTNWQPDPARAVLLIHDMQRYFLRFYDANGPLMGDLIGNL
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VRLRNWAHENGVPVVYTAQPHEQPAGYRALLNDMWGPGLPAAAPEQQLIVDKLTPGATDT
HHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCHH
VLAKWRYSAFQRSDLLQRMKDWNRDQLIIGGIYAHIGCMVTAIDAFMNDIQPFMVGDAVA
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
DFSEEEHRLALKYVATRCGAVIDTASLIRKPATETTRDWLQKRVLELIEDETELDPQENL
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHCCCCCCCE
ILYGLDSLQVMKLSAELKERGVAVGFDDLARVPTLAAWWDLIETKQKAAA
EEEECCCHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]