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The map label for this gene is pyrG [H]

Identifier: 225630105

GI number: 225630105

Start: 298055

End: 299659

Strand: Direct

Name: pyrG [H]

Synonym: WRi_002850

Alternate gene names: 225630105

Gene position: 298055-299659 (Clockwise)

Preceding gene: 225630104

Following gene: 225630106

Centisome position: 20.61

GC content: 37.88

Gene sequence:

>1605_bases
ATGAAGGAAGCTAAATTTATCTTTGTCACAGGTGGAGTTGTGTCATCGCTTGGTAAAGGCTTAGTTGCTTCGAGTGTTGG
TGCGCTTCTTCAAGCTCACGGTTTTAAGATCCGTATCAGAAAACTTGACCCGTATCTCAACATTGATCCTGGGACAATGA
ACCCAACTCAGCACGGAGAGGTATTTGTTACCGAAGATGGTGCTGAAACTGATTTGGATCTTGGGCATTATGAGCGTTTT
ACTGGAATTAAAGCAACAAAAGATGACAATATAACAACTGGTAAGATATATCATGAGTTATTGAAGAAAGAGAGGCGTGG
TGATTATCTGGGCAAAACTGTGCAAGTCATTCCTCATGTAACAGATTTGATCAAATCGTTCATTTTTAATGGTACGGAAG
GTTTAGATTTTGTAATATGTGAAATAGGCGGAACTGTAGGTGACATTGAAAGCCAACCATTTTTGGAGGCTATACGCCAA
GTTAACTACACATTAGGAAAACAAAGAGTTATCCTTATTCACTTAACTTTAATACCATATCTTACCGCAGCGCAAGAATT
AAAGACAAAGCCAACGCAGCATTCAGTTCGAGAGTTAAATTCCGCGGGGTTACAACCAGATATTATATTATGCCGCAGTG
AGAAAGAAATTTTCGATAATCAAAGAGAGAAAATAGCTAAGCTTTGCAATGTTTCTTTATCCAATGTGATACCTGCCCCT
GATGTGAGTCATATATATGAGTTACCGGTGTTGTATAGCCAATGTGGGCTTGATACGCAAATTTTGGAGCACTTTCATTT
AAGTAAGCCAAAACCAAGCTTGACTGAATGGGATCAAATAGTGCATTCCATAAGGCACCCAACACAGGAAGTTACTGTAT
CTATAGTGGGAAAATACACCGAATTCCCTGATGCATACAAATCACTGGTTGAAGCATTAAATCATGGTGCGATTAGTAAT
AAAGTCAAAGTAAAGATAAATTGGGTTAACTCAAGAGAGAAGGAAGAAAAACCTATAAATGAAAAACTTATAGGAGAGAA
ATTACAGAATTCTCATGCAATCCTTGTTCCAGGGGGGTTTGGTGATGACGGAGTAGAGGGTAAAATATTAGCAATAAATT
ATGCCCGTACAAATAATATCCCATTTTTCGGAATTTGCCTTGGTATGCAGCTTGCAATTATTGAATTTGCTCGTAATGTT
GTTAAGCTTGAAGATGCACACTCTGAAGAATTTCGTAACTGTAAACATCCAATCGTTAAGTTAGCTGGCGATCAAGATGA
CGATCTTGGTGGAACCATGAGACTTGGGGCATACAAATGTAATATAAATGCAAACTCTAAAATGATGGATGCATATAGTA
ACACTACTATTTCAGAAAGACACAGGCATAGATACATAATCAATTCAGATTATAAAGATGATTTGGAAAAAAATGGGCTG
CTATGCAGTGGCATATCAGAAGATGGAACGTGCATAGAGGCAGTGGAGTTAGAGAGTCATCCGTGGTTTATTGGTGTGCA
GTTTCATCCAGAGTTTCAATCAAAGCCGTTTTCTCCTCATCCTCTTTTTGTATCATTTATTGAAGCAGCAATTGATAAGA
AATAA

Upstream 100 bases:

>100_bases
GATTATGTTCAAAAGACGTACATAAAAAATCGATCGCAGAAAAAATTATATTAGAAAAAAAGCAAGAAAGTGAATCTACT
TCTGTTCCATTTGAAAATTA

Downstream 100 bases:

>100_bases
GTGCCTAGTGTTTAAGTATCAATATATGGAGCTTGCCATAGAGCAAGCCAAACTCGCTCAAAAAAATGATGAGGTGCCCA
TAGGTGCTGTAATAGTGAGT

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 534; Mature: 534

Protein sequence:

>534_residues
MKEAKFIFVTGGVVSSLGKGLVASSVGALLQAHGFKIRIRKLDPYLNIDPGTMNPTQHGEVFVTEDGAETDLDLGHYERF
TGIKATKDDNITTGKIYHELLKKERRGDYLGKTVQVIPHVTDLIKSFIFNGTEGLDFVICEIGGTVGDIESQPFLEAIRQ
VNYTLGKQRVILIHLTLIPYLTAAQELKTKPTQHSVRELNSAGLQPDIILCRSEKEIFDNQREKIAKLCNVSLSNVIPAP
DVSHIYELPVLYSQCGLDTQILEHFHLSKPKPSLTEWDQIVHSIRHPTQEVTVSIVGKYTEFPDAYKSLVEALNHGAISN
KVKVKINWVNSREKEEKPINEKLIGEKLQNSHAILVPGGFGDDGVEGKILAINYARTNNIPFFGICLGMQLAIIEFARNV
VKLEDAHSEEFRNCKHPIVKLAGDQDDDLGGTMRLGAYKCNINANSKMMDAYSNTTISERHRHRYIINSDYKDDLEKNGL
LCSGISEDGTCIEAVELESHPWFIGVQFHPEFQSKPFSPHPLFVSFIEAAIDKK

Sequences:

>Translated_534_residues
MKEAKFIFVTGGVVSSLGKGLVASSVGALLQAHGFKIRIRKLDPYLNIDPGTMNPTQHGEVFVTEDGAETDLDLGHYERF
TGIKATKDDNITTGKIYHELLKKERRGDYLGKTVQVIPHVTDLIKSFIFNGTEGLDFVICEIGGTVGDIESQPFLEAIRQ
VNYTLGKQRVILIHLTLIPYLTAAQELKTKPTQHSVRELNSAGLQPDIILCRSEKEIFDNQREKIAKLCNVSLSNVIPAP
DVSHIYELPVLYSQCGLDTQILEHFHLSKPKPSLTEWDQIVHSIRHPTQEVTVSIVGKYTEFPDAYKSLVEALNHGAISN
KVKVKINWVNSREKEEKPINEKLIGEKLQNSHAILVPGGFGDDGVEGKILAINYARTNNIPFFGICLGMQLAIIEFARNV
VKLEDAHSEEFRNCKHPIVKLAGDQDDDLGGTMRLGAYKCNINANSKMMDAYSNTTISERHRHRYIINSDYKDDLEKNGL
LCSGISEDGTCIEAVELESHPWFIGVQFHPEFQSKPFSPHPLFVSFIEAAIDKK
>Mature_534_residues
MKEAKFIFVTGGVVSSLGKGLVASSVGALLQAHGFKIRIRKLDPYLNIDPGTMNPTQHGEVFVTEDGAETDLDLGHYERF
TGIKATKDDNITTGKIYHELLKKERRGDYLGKTVQVIPHVTDLIKSFIFNGTEGLDFVICEIGGTVGDIESQPFLEAIRQ
VNYTLGKQRVILIHLTLIPYLTAAQELKTKPTQHSVRELNSAGLQPDIILCRSEKEIFDNQREKIAKLCNVSLSNVIPAP
DVSHIYELPVLYSQCGLDTQILEHFHLSKPKPSLTEWDQIVHSIRHPTQEVTVSIVGKYTEFPDAYKSLVEALNHGAISN
KVKVKINWVNSREKEEKPINEKLIGEKLQNSHAILVPGGFGDDGVEGKILAINYARTNNIPFFGICLGMQLAIIEFARNV
VKLEDAHSEEFRNCKHPIVKLAGDQDDDLGGTMRLGAYKCNINANSKMMDAYSNTTISERHRHRYIINSDYKDDLEKNGL
LCSGISEDGTCIEAVELESHPWFIGVQFHPEFQSKPFSPHPLFVSFIEAAIDKK

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=549, Percent_Identity=44.08014571949, Blast_Score=476, Evalue=1e-134,
Organism=Homo sapiens, GI28559085, Length=546, Percent_Identity=44.3223443223443, Blast_Score=459, Evalue=1e-129,
Organism=Homo sapiens, GI28559083, Length=546, Percent_Identity=44.3223443223443, Blast_Score=459, Evalue=1e-129,
Organism=Homo sapiens, GI221316689, Length=546, Percent_Identity=44.3223443223443, Blast_Score=459, Evalue=1e-129,
Organism=Escherichia coli, GI1789142, Length=538, Percent_Identity=52.9739776951673, Blast_Score=574, Evalue=1e-165,
Organism=Caenorhabditis elegans, GI25148299, Length=603, Percent_Identity=38.6401326699834, Blast_Score=414, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6319432, Length=562, Percent_Identity=41.2811387900356, Blast_Score=429, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6322563, Length=563, Percent_Identity=40.3197158081705, Blast_Score=422, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24664469, Length=549, Percent_Identity=43.3515482695811, Blast_Score=457, Evalue=1e-129,
Organism=Drosophila melanogaster, GI21357815, Length=494, Percent_Identity=42.1052631578947, Blast_Score=392, Evalue=1e-109,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 59672; Mature: 59672

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEAKFIFVTGGVVSSLGKGLVASSVGALLQAHGFKIRIRKLDPYLNIDPGTMNPTQHGE
CCCCCEEEEECHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCE
VFVTEDGAETDLDLGHYERFTGIKATKDDNITTGKIYHELLKKERRGDYLGKTVQVIPHV
EEEECCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHCHHHHHHHHH
TDLIKSFIFNGTEGLDFVICEIGGTVGDIESQPFLEAIRQVNYTLGKQRVILIHLTLIPY
HHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEHHHHH
LTAAQELKTKPTQHSVRELNSAGLQPDIILCRSEKEIFDNQREKIAKLCNVSLSNVIPAP
HHHHHHHHCCCHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHCCHHHHCCCCC
DVSHIYELPVLYSQCGLDTQILEHFHLSKPKPSLTEWDQIVHSIRHPTQEVTVSIVGKYT
CHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHEEEEEEECCC
EFPDAYKSLVEALNHGAISNKVKVKINWVNSREKEEKPINEKLIGEKLQNSHAILVPGGF
CCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEECCCC
GDDGVEGKILAINYARTNNIPFFGICLGMQLAIIEFARNVVKLEDAHSEEFRNCKHPIVK
CCCCCCCEEEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEE
LAGDQDDDLGGTMRLGAYKCNINANSKMMDAYSNTTISERHRHRYIINSDYKDDLEKNGL
ECCCCCCCCCCEEEEEEEEEECCCCCHHHHHHCCCCHHHHHCCEEEECCCCHHHHHHCCE
LCSGISEDGTCIEAVELESHPWFIGVQFHPEFQSKPFSPHPLFVSFIEAAIDKK
EEECCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKEAKFIFVTGGVVSSLGKGLVASSVGALLQAHGFKIRIRKLDPYLNIDPGTMNPTQHGE
CCCCCEEEEECHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCE
VFVTEDGAETDLDLGHYERFTGIKATKDDNITTGKIYHELLKKERRGDYLGKTVQVIPHV
EEEECCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHCHHHHHHHHH
TDLIKSFIFNGTEGLDFVICEIGGTVGDIESQPFLEAIRQVNYTLGKQRVILIHLTLIPY
HHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEHHHHH
LTAAQELKTKPTQHSVRELNSAGLQPDIILCRSEKEIFDNQREKIAKLCNVSLSNVIPAP
HHHHHHHHCCCHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHCCHHHHCCCCC
DVSHIYELPVLYSQCGLDTQILEHFHLSKPKPSLTEWDQIVHSIRHPTQEVTVSIVGKYT
CHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHEEEEEEECCC
EFPDAYKSLVEALNHGAISNKVKVKINWVNSREKEEKPINEKLIGEKLQNSHAILVPGGF
CCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEECCCC
GDDGVEGKILAINYARTNNIPFFGICLGMQLAIIEFARNVVKLEDAHSEEFRNCKHPIVK
CCCCCCCEEEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEE
LAGDQDDDLGGTMRLGAYKCNINANSKMMDAYSNTTISERHRHRYIINSDYKDDLEKNGL
ECCCCCCCCCCEEEEEEEEEECCCCCHHHHHHCCCCHHHHHCCEEEECCCCHHHHHHCCE
LCSGISEDGTCIEAVELESHPWFIGVQFHPEFQSKPFSPHPLFVSFIEAAIDKK
EEECCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA